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The map label for this gene is rsuA [H]

Identifier: 73540410

GI number: 73540410

Start: 765037

End: 765792

Strand: Direct

Name: rsuA [H]

Synonym: Reut_A0704

Alternate gene names: 73540410

Gene position: 765037-765792 (Clockwise)

Preceding gene: 73540408

Following gene: 73540411

Centisome position: 20.1

GC content: 64.15

Gene sequence:

>756_bases
ATGGCCCAGCCCTCCGAAACATTTTCACTGACGATTCAATTTCCCATGACCCTCGACCGCATCCTCCAATCCCAAGGCTT
TGGCACACGCCGCTACTGTGGCGACTTGATCGCTGCGGGGCTGGTGGAGGTCAATGGCGAGCTCTGCGAAGACCCACGTG
CGCAGTTCGAGGTTGACGGACTGCGCCTGACTGTCGATGGTGAGGAATGGTTGGCCTGCACCAAGGCCTACCTGATGCTG
AACAAGCCGGCCGGCTACGAGTGCTCGCAGCGCCCGCGCCACCACCCGAGCGTGTACAACCTGCTCCCGGTGCCGCTGCG
CCAGCGCGAGGTGCAGGCCGTCGGCCGGCTCGACCACGACACCACGGGCTTGCTGTTGCTGACGGACGATGGGCAGTTCA
TCCACGCCCAGACTTCGCCAAAGCGCAAGGTGCCGAAGGTCTACGAGGTGACGACGGCCGAGCCGGTCACGCAAGAACAG
GTGGACGCCCTGCGCACTGGCGTGAAACTCGACGATGAACCGGTGCCGATCGCCGCAGAAGGGTGCGAAATCACTGGCGA
ATGCAGCCTGCGCCTGACGCTGCTGCAAGGCAAGTACCACCAGGTCAAGCGCATGGTGGCCGCTGCCGGCAATCACGTGA
ATGCCTTGCATCGCAGTGCGGTCGGCGGCTTGAGGCTGGATCCAGCGGCGCTGCCAGAAGGTGAGTGGCGCTGGCTGACG
GCCGAGGATCTGGCGGCCTTGACCAGCAAGGACTGA

Upstream 100 bases:

>100_bases
GTCGATGCGGTAGGGTGGGCGAGCGTGGGCTGGTGTGTCTGCGCCTTGCATGGTGCCTTGGTGAAGGTTAGCCGGGCGAT
ACCCTAACATAGCGATACGG

Downstream 100 bases:

>100_bases
GCCACCGTTTGCTGCGCCGCAGCGCGGCCAATTCTTTTCGGGTCGCCTCCCAGTGATTTGGTAAAATGCCACGTATGGCC
AAGTCCGAAGCTCCCATCAA

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MAQPSETFSLTIQFPMTLDRILQSQGFGTRRYCGDLIAAGLVEVNGELCEDPRAQFEVDGLRLTVDGEEWLACTKAYLML
NKPAGYECSQRPRHHPSVYNLLPVPLRQREVQAVGRLDHDTTGLLLLTDDGQFIHAQTSPKRKVPKVYEVTTAEPVTQEQ
VDALRTGVKLDDEPVPIAAEGCEITGECSLRLTLLQGKYHQVKRMVAAAGNHVNALHRSAVGGLRLDPAALPEGEWRWLT
AEDLAALTSKD

Sequences:

>Translated_251_residues
MAQPSETFSLTIQFPMTLDRILQSQGFGTRRYCGDLIAAGLVEVNGELCEDPRAQFEVDGLRLTVDGEEWLACTKAYLML
NKPAGYECSQRPRHHPSVYNLLPVPLRQREVQAVGRLDHDTTGLLLLTDDGQFIHAQTSPKRKVPKVYEVTTAEPVTQEQ
VDALRTGVKLDDEPVPIAAEGCEITGECSLRLTLLQGKYHQVKRMVAAAGNHVNALHRSAVGGLRLDPAALPEGEWRWLT
AEDLAALTSKD
>Mature_250_residues
AQPSETFSLTIQFPMTLDRILQSQGFGTRRYCGDLIAAGLVEVNGELCEDPRAQFEVDGLRLTVDGEEWLACTKAYLMLN
KPAGYECSQRPRHHPSVYNLLPVPLRQREVQAVGRLDHDTTGLLLLTDDGQFIHAQTSPKRKVPKVYEVTTAEPVTQEQV
DALRTGVKLDDEPVPIAAEGCEITGECSLRLTLLQGKYHQVKRMVAAAGNHVNALHRSAVGGLRLDPAALPEGEWRWLTA
EDLAALTSKD

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=238, Percent_Identity=39.4957983193277, Blast_Score=147, Evalue=4e-37,
Organism=Escherichia coli, GI1787524, Length=239, Percent_Identity=33.0543933054393, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1790453, Length=221, Percent_Identity=28.9592760180996, Blast_Score=77, Evalue=7e-16,
Organism=Escherichia coli, GI87081838, Length=149, Percent_Identity=36.241610738255, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 27711; Mature: 27580

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQPSETFSLTIQFPMTLDRILQSQGFGTRRYCGDLIAAGLVEVNGELCEDPRAQFEVDG
CCCCCCEEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHCCCCCCEEEECC
LRLTVDGEEWLACTKAYLMLNKPAGYECSQRPRHHPSVYNLLPVPLRQREVQAVGRLDHD
EEEEECCHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCC
TTGLLLLTDDGQFIHAQTSPKRKVPKVYEVTTAEPVTQEQVDALRTGVKLDDEPVPIAAE
CCEEEEEECCCCEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCEEEC
GCEITGECSLRLTLLQGKYHQVKRMVAAAGNHVNALHRSAVGGLRLDPAALPEGEWRWLT
CCEEECCCEEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHCCCCEECCCCCCCCCEEEEC
AEDLAALTSKD
HHHHHHHHCCC
>Mature Secondary Structure 
AQPSETFSLTIQFPMTLDRILQSQGFGTRRYCGDLIAAGLVEVNGELCEDPRAQFEVDG
CCCCCEEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHCCCCCCEEEECC
LRLTVDGEEWLACTKAYLMLNKPAGYECSQRPRHHPSVYNLLPVPLRQREVQAVGRLDHD
EEEEECCHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCC
TTGLLLLTDDGQFIHAQTSPKRKVPKVYEVTTAEPVTQEQVDALRTGVKLDDEPVPIAAE
CCEEEEEECCCCEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCEEEC
GCEITGECSLRLTLLQGKYHQVKRMVAAAGNHVNALHRSAVGGLRLDPAALPEGEWRWLT
CCEEECCCEEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHCCCCEECCCCCCCCCEEEEC
AEDLAALTSKD
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]