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The map label for this gene is paaF [C]

Identifier: 73540353

GI number: 73540353

Start: 696035

End: 696817

Strand: Direct

Name: paaF [C]

Synonym: Reut_A0647

Alternate gene names: 73540353

Gene position: 696035-696817 (Clockwise)

Preceding gene: 73540352

Following gene: 73540354

Centisome position: 18.29

GC content: 66.92

Gene sequence:

>783_bases
ATGAGCGGTATTGTGCTTTTCGCCGTCGAGGGCACGACGGCCACCGTCACGCTGTCGCATTCGGGCAAGCTCAACGCGAT
ATCGGCGCAGATGTGGCAGGCGCTGGCCGACGGCTTTACGCGGCTGTCGGCCGATACTTCGCTGCGCTGCGTGGTGGTGC
GCGGCGCCGACGGCAATTTTGCCGCTGGCGCCGACATTGCCGAGTTTCCAGTGGTGCGTGGCGATGAGGCTGCGGTACGC
CGCTACCACATGGAAATCATCGCTCCGGCGCTGCAAGCCATTGCCAATTGCCTGCACCCGACCGTTGCGAAGATCGAGGG
CGTATGTGTCGGTGGTGGACTGGAGATCGCCTGCCATTGTGACCTGCGCATCGCTGCGGATAACAGCCGCTTCGGAGTGC
CGATCAACCGGCTCGGTTTCCCGATGGCGCCGGGCGAACTGCAAGGCCTGCTGGCGCTCGCCGGTCGCGCCGTCGCGCTC
GAGATCCTGCTTGAGGGCCGTGTCTTCGATGCAGCCGAAGCACGCGACAAAGGCCTGCTGACACGCGTTGTGCCGGCTGG
CGCACTGGCGGCGGAAGTCGACGCCGCCGTGCGCCGCATCACGGCCGGTGCCCCGGCCGCGGCGCGCATCAACAAGGCCA
CGATCCGGCGGCTTTCGCCGCAGGCGCAACCGCTGAATGCCGAGGAGCTCGACGCGCACTTTACCTATGCCGAAGGCGGA
GACCATGCCGAGGGCGTGGCCGCGTTCCTTGCGCGCCGGCCGCCGCAATTCGAAGGGAAGTAG

Upstream 100 bases:

>100_bases
GCCGCAATTCACGCAGGAGCAGTTCGCCCAGTTCCAGCGCAACGAGATCAAGCGCTGGGCGCAAGTGGTGCAACTCTCGG
GCGCCAGGATCGACTGAGCG

Downstream 100 bases:

>100_bases
CGCATTTCGTTGCCAGATTTCCCGATTACAATCGTCCATCGCTCACACGCGCCCGGAGCCCGGATCCGGAAGCTACCGTC
TTCATCCGCACCATGAACGC

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MSGIVLFAVEGTTATVTLSHSGKLNAISAQMWQALADGFTRLSADTSLRCVVVRGADGNFAAGADIAEFPVVRGDEAAVR
RYHMEIIAPALQAIANCLHPTVAKIEGVCVGGGLEIACHCDLRIAADNSRFGVPINRLGFPMAPGELQGLLALAGRAVAL
EILLEGRVFDAAEARDKGLLTRVVPAGALAAEVDAAVRRITAGAPAAARINKATIRRLSPQAQPLNAEELDAHFTYAEGG
DHAEGVAAFLARRPPQFEGK

Sequences:

>Translated_260_residues
MSGIVLFAVEGTTATVTLSHSGKLNAISAQMWQALADGFTRLSADTSLRCVVVRGADGNFAAGADIAEFPVVRGDEAAVR
RYHMEIIAPALQAIANCLHPTVAKIEGVCVGGGLEIACHCDLRIAADNSRFGVPINRLGFPMAPGELQGLLALAGRAVAL
EILLEGRVFDAAEARDKGLLTRVVPAGALAAEVDAAVRRITAGAPAAARINKATIRRLSPQAQPLNAEELDAHFTYAEGG
DHAEGVAAFLARRPPQFEGK
>Mature_259_residues
SGIVLFAVEGTTATVTLSHSGKLNAISAQMWQALADGFTRLSADTSLRCVVVRGADGNFAAGADIAEFPVVRGDEAAVRR
YHMEIIAPALQAIANCLHPTVAKIEGVCVGGGLEIACHCDLRIAADNSRFGVPINRLGFPMAPGELQGLLALAGRAVALE
ILLEGRVFDAAEARDKGLLTRVVPAGALAAEVDAAVRRITAGAPAAARINKATIRRLSPQAQPLNAEELDAHFTYAEGGD
HAEGVAAFLARRPPQFEGK

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=254, Percent_Identity=28.3464566929134, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI70995211, Length=210, Percent_Identity=28.0952380952381, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI194097323, Length=252, Percent_Identity=23.015873015873, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI68989263, Length=176, Percent_Identity=33.5227272727273, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI4502327, Length=249, Percent_Identity=29.3172690763052, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI20127408, Length=183, Percent_Identity=30.0546448087432, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1787659, Length=246, Percent_Identity=29.2682926829268, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787660, Length=268, Percent_Identity=28.7313432835821, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082183, Length=256, Percent_Identity=27.34375, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788597, Length=253, Percent_Identity=29.2490118577075, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI221142681, Length=266, Percent_Identity=26.6917293233083, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788682, Length=205, Percent_Identity=31.219512195122, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1790281, Length=239, Percent_Identity=25.1046025104602, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17554946, Length=252, Percent_Identity=30.5555555555556, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI25145438, Length=246, Percent_Identity=28.0487804878049, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17560910, Length=201, Percent_Identity=29.8507462686567, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=176, Percent_Identity=29.5454545454545, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17536985, Length=216, Percent_Identity=25.9259259259259, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=261, Percent_Identity=24.904214559387, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=263, Percent_Identity=25.4752851711027, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=207, Percent_Identity=26.0869565217391, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17558304, Length=166, Percent_Identity=30.7228915662651, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=210, Percent_Identity=26.6666666666667, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI20129971, Length=247, Percent_Identity=29.1497975708502, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24653477, Length=247, Percent_Identity=29.1497975708502, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24653139, Length=257, Percent_Identity=29.5719844357977, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19922422, Length=262, Percent_Identity=25.5725190839695, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19920382, Length=252, Percent_Identity=26.5873015873016, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24650670, Length=195, Percent_Identity=33.8461538461538, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21357171, Length=186, Percent_Identity=30.1075268817204, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27123; Mature: 26992

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGIVLFAVEGTTATVTLSHSGKLNAISAQMWQALADGFTRLSADTSLRCVVVRGADGNF
CCCEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCC
AAGADIAEFPVVRGDEAAVRRYHMEIIAPALQAIANCLHPTVAKIEGVCVGGGLEIACHC
CCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCEEECCCEEEEEEE
DLRIAADNSRFGVPINRLGFPMAPGELQGLLALAGRAVALEILLEGRVFDAAEARDKGLL
EEEEEECCCCCCCCHHHCCCCCCCHHHHHHHHHHCHHEEEEEEECCCEECCHHHHCCCCE
TRVVPAGALAAEVDAAVRRITAGAPAAARINKATIRRLSPQAQPLNAEELDAHFTYAEGG
EECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCEEEECCCC
DHAEGVAAFLARRPPQFEGK
CCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SGIVLFAVEGTTATVTLSHSGKLNAISAQMWQALADGFTRLSADTSLRCVVVRGADGNF
CCEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCC
AAGADIAEFPVVRGDEAAVRRYHMEIIAPALQAIANCLHPTVAKIEGVCVGGGLEIACHC
CCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCEEECCCEEEEEEE
DLRIAADNSRFGVPINRLGFPMAPGELQGLLALAGRAVALEILLEGRVFDAAEARDKGLL
EEEEEECCCCCCCCHHHCCCCCCCHHHHHHHHHHCHHEEEEEEECCCEECCHHHHCCCCE
TRVVPAGALAAEVDAAVRRITAGAPAAARINKATIRRLSPQAQPLNAEELDAHFTYAEGG
EECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCEEEECCCC
DHAEGVAAFLARRPPQFEGK
CCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA