Click here to switch to the map view.

The map label for this gene is dacB [H]

Identifier: 73540270

GI number: 73540270

Start: 610633

End: 612147

Strand: Reverse

Name: dacB [H]

Synonym: Reut_A0564

Alternate gene names: 73540270

Gene position: 612147-610633 (Counterclockwise)

Preceding gene: 73540271

Following gene: 73540269

Centisome position: 16.08

GC content: 68.91

Gene sequence:

>1515_bases
ATGGCCGCCATGCCAAAAAGAATCACCGTATTTTGTGCGGCAGCCCTGCTGCTCGCCGCCACCCAGCCGCCCGTTCTGGC
CCGCCCGGCCAAGGCGATCAAGACTTCCGCCAGCGCGCGGGCGGATGGTGCGCTCGTACGCGCCGGCTTGCCGGCCCCGG
TCGCCACGGCGCTGCAGCGCGCGCACGTGCCGGCCAGCGCGGCCAGTTTCTATGTCGTGAAAATCGGGGCGCCGCAGGCG
CGCGCAAGCTGGAACGCGCAGCAGCCGATGAACCCCGCTTCGACCATGAAGCTGGTCACCACGTTTGCCGGACTGCAGTT
GCTGGGCCCCGACTACCGCTGGCAGACCTCGCTCTATGCCGACAGCCAGCCCGCCGGCGACGGGACGGTCAACGGCAATG
TCTACCTGCGCGGACGCGGCGACCCGAAGCTGGTGCCGGAGGAAATGGCCAAGCTCGTCGCCACCGCGCGCGCGGCCGGC
GCCACCACGATCAATGGCGACCTGGTGCTTGACCGCAGCTTCTTTGCCGATGGCCTGAACGGGAACGGCACCATCGATGG
CGAAGTCCAGCGCGCGTACAACGTCAGCCCCGACGCACTGCTGTACGCGTTCAAGACGCTGTCGTTCACGCTCACCCCCA
ACGATGCCAGCCAGACCGTGGCGGTCTCGGTCACGCCGGCGCTCGCGCAACTGCGCCTGGACAACCGCCTGACGCTGACC
AATGGCCGCTGCGGCGACTGGCAATCGAGCGCCACGCCGACCGTGGCGCCGCAGGCCGACGGCACGGTGCTGGCGTCGTT
CTCGGGCAACTATTCGAGCGACTGCGGCGAGCACGTGCTCAATATCGCGGCGCTGTCGCATGCCGAGTTCACGTGGGGCG
GCTTCGTGGCCGAGTGGCAGGCCGCGGGCGGCCGTTTCACGCACGTGCCGGCGATGCGCAGCGGCTCCGTCCCGCGCGGC
GCGGTGCTGCTGGCGCGCCACTACGGCCAGCCGCTCGGCGCGATCGTGCGCGACATCAACAAGTATTCGAACAACGTGAT
GGCGCGCCAGCTGTTCCTGACCATCGGCGCGGAGATGGATCGCAGCGGGCCGGCCAGCACCGCGCGCTCCACGCGCGTAG
TGCAGCGCTGGCTGAGCCGCCAGGGCCTGGATATGCAGGGACTGGTGCTCGACAACGGCTCGGGCCTGTCGCGCGACGAA
CGTATCAGCGCCTACGACATGGCGCGGCTGCTGCAGCAGGCGGCCGCGAGCAACGTCGGGCCGACGCTGGTCGATTCGCT
GCCGATCCTGGGCGTCGACGGCACCTTGCGCAACCGGCTCACGCGCGCCAACGCCGCCGGCAATGCTTACCTCAAGACCG
GCACGCTGCAGGACGTACGCGCGCTGGCCGGCTATGTCGATGCGCTCAATGGCGAACGTTACGTGGTGGTGAGCTTTATC
AACCACCCGAACGCCTCACAGGCGCAGGAAGCCCATGATGCGCTGATGCAGTGGGTGTATCGCGGCGCACCCTGA

Upstream 100 bases:

>100_bases
GCGCCTGGCGCCGCCGCGCCCGGAGTAGCCGCAGGCCGGTCTGACTGAACCATCAGCGCCGGCACGGGTGTCATCCGTCA
GCCACCGGCCATGCGTTAAC

Downstream 100 bases:

>100_bases
AGCACCAGCGGCACGGGGATGCGCAGGCCGGCGTGGACGCCGGCCAACGCCGCCCGGGAACTTGTTAAGTATCTACTTAC
CAGCGCCGCGTCCGACCCTA

Product: D-alanyl-D-alanine carboxypeptidase PBP3

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MAAMPKRITVFCAAALLLAATQPPVLARPAKAIKTSASARADGALVRAGLPAPVATALQRAHVPASAASFYVVKIGAPQA
RASWNAQQPMNPASTMKLVTTFAGLQLLGPDYRWQTSLYADSQPAGDGTVNGNVYLRGRGDPKLVPEEMAKLVATARAAG
ATTINGDLVLDRSFFADGLNGNGTIDGEVQRAYNVSPDALLYAFKTLSFTLTPNDASQTVAVSVTPALAQLRLDNRLTLT
NGRCGDWQSSATPTVAPQADGTVLASFSGNYSSDCGEHVLNIAALSHAEFTWGGFVAEWQAAGGRFTHVPAMRSGSVPRG
AVLLARHYGQPLGAIVRDINKYSNNVMARQLFLTIGAEMDRSGPASTARSTRVVQRWLSRQGLDMQGLVLDNGSGLSRDE
RISAYDMARLLQQAAASNVGPTLVDSLPILGVDGTLRNRLTRANAAGNAYLKTGTLQDVRALAGYVDALNGERYVVVSFI
NHPNASQAQEAHDALMQWVYRGAP

Sequences:

>Translated_504_residues
MAAMPKRITVFCAAALLLAATQPPVLARPAKAIKTSASARADGALVRAGLPAPVATALQRAHVPASAASFYVVKIGAPQA
RASWNAQQPMNPASTMKLVTTFAGLQLLGPDYRWQTSLYADSQPAGDGTVNGNVYLRGRGDPKLVPEEMAKLVATARAAG
ATTINGDLVLDRSFFADGLNGNGTIDGEVQRAYNVSPDALLYAFKTLSFTLTPNDASQTVAVSVTPALAQLRLDNRLTLT
NGRCGDWQSSATPTVAPQADGTVLASFSGNYSSDCGEHVLNIAALSHAEFTWGGFVAEWQAAGGRFTHVPAMRSGSVPRG
AVLLARHYGQPLGAIVRDINKYSNNVMARQLFLTIGAEMDRSGPASTARSTRVVQRWLSRQGLDMQGLVLDNGSGLSRDE
RISAYDMARLLQQAAASNVGPTLVDSLPILGVDGTLRNRLTRANAAGNAYLKTGTLQDVRALAGYVDALNGERYVVVSFI
NHPNASQAQEAHDALMQWVYRGAP
>Mature_503_residues
AAMPKRITVFCAAALLLAATQPPVLARPAKAIKTSASARADGALVRAGLPAPVATALQRAHVPASAASFYVVKIGAPQAR
ASWNAQQPMNPASTMKLVTTFAGLQLLGPDYRWQTSLYADSQPAGDGTVNGNVYLRGRGDPKLVPEEMAKLVATARAAGA
TTINGDLVLDRSFFADGLNGNGTIDGEVQRAYNVSPDALLYAFKTLSFTLTPNDASQTVAVSVTPALAQLRLDNRLTLTN
GRCGDWQSSATPTVAPQADGTVLASFSGNYSSDCGEHVLNIAALSHAEFTWGGFVAEWQAAGGRFTHVPAMRSGSVPRGA
VLLARHYGQPLGAIVRDINKYSNNVMARQLFLTIGAEMDRSGPASTARSTRVVQRWLSRQGLDMQGLVLDNGSGLSRDER
ISAYDMARLLQQAAASNVGPTLVDSLPILGVDGTLRNRLTRANAAGNAYLKTGTLQDVRALAGYVDALNGERYVVVSFIN
HPNASQAQEAHDALMQWVYRGAP

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=470, Percent_Identity=27.8723404255319, Blast_Score=133, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 53302; Mature: 53171

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAMPKRITVFCAAALLLAATQPPVLARPAKAIKTSASARADGALVRAGLPAPVATALQR
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCEEECCCCCHHHHHHHH
AHVPASAASFYVVKIGAPQARASWNAQQPMNPASTMKLVTTFAGLQLLGPDYRWQTSLYA
HCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHEECCCCCEEEEEEC
DSQPAGDGTVNGNVYLRGRGDPKLVPEEMAKLVATARAAGATTINGDLVLDRSFFADGLN
CCCCCCCCEECCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEECCCEEEEHHHHHCCCC
GNGTIDGEVQRAYNVSPDALLYAFKTLSFTLTPNDASQTVAVSVTPALAQLRLDNRLTLT
CCCCCCCCHHHCCCCCHHHHHHEEEHEEEEECCCCCCCEEEEEECHHHHHHCCCCEEEEC
NGRCGDWQSSATPTVAPQADGTVLASFSGNYSSDCGEHVLNIAALSHAEFTWGGFVAEWQ
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEHH
AAGGRFTHVPAMRSGSVPRGAVLLARHYGQPLGAIVRDINKYSNNVMARQLFLTIGAEMD
HCCCEEEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RSGPASTARSTRVVQRWLSRQGLDMQGLVLDNGSGLSRDERISAYDMARLLQQAAASNVG
CCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCC
PTLVDSLPILGVDGTLRNRLTRANAAGNAYLKTGTLQDVRALAGYVDALNGERYVVVSFI
CHHHHCCCEEECCHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEEEEEEE
NHPNASQAQEAHDALMQWVYRGAP
CCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AAMPKRITVFCAAALLLAATQPPVLARPAKAIKTSASARADGALVRAGLPAPVATALQR
CCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCEEECCCCCHHHHHHHH
AHVPASAASFYVVKIGAPQARASWNAQQPMNPASTMKLVTTFAGLQLLGPDYRWQTSLYA
HCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHEECCCCCEEEEEEC
DSQPAGDGTVNGNVYLRGRGDPKLVPEEMAKLVATARAAGATTINGDLVLDRSFFADGLN
CCCCCCCCEECCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEECCCEEEEHHHHHCCCC
GNGTIDGEVQRAYNVSPDALLYAFKTLSFTLTPNDASQTVAVSVTPALAQLRLDNRLTLT
CCCCCCCCHHHCCCCCHHHHHHEEEHEEEEECCCCCCCEEEEEECHHHHHHCCCCEEEEC
NGRCGDWQSSATPTVAPQADGTVLASFSGNYSSDCGEHVLNIAALSHAEFTWGGFVAEWQ
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEHH
AAGGRFTHVPAMRSGSVPRGAVLLARHYGQPLGAIVRDINKYSNNVMARQLFLTIGAEMD
HCCCEEEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RSGPASTARSTRVVQRWLSRQGLDMQGLVLDNGSGLSRDERISAYDMARLLQQAAASNVG
CCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCC
PTLVDSLPILGVDGTLRNRLTRANAAGNAYLKTGTLQDVRALAGYVDALNGERYVVVSFI
CHHHHCCCEEECCHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEEEEEEE
NHPNASQAQEAHDALMQWVYRGAP
CCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2040429; 9278503; 2046551 [H]