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The map label for this gene is proP [H]

Identifier: 73540229

GI number: 73540229

Start: 568706

End: 570403

Strand: Reverse

Name: proP [H]

Synonym: Reut_A0523

Alternate gene names: 73540229

Gene position: 570403-568706 (Counterclockwise)

Preceding gene: 73540230

Following gene: 73540225

Centisome position: 14.98

GC content: 63.84

Gene sequence:

>1698_bases
ATGGCAAACACGCAAAACGTAGCGATGCCCGGCGGAGGCGCGCAGAACGCACCGATGACAACCGAGGAGAAGAAGGTCAT
TCTGGCCTCGTCGCTCGGCACGGTGTTCGAGTGGTATGACTTTTACCTGTACGGCTCGCTGGCGGCAGTGATCGCCAAGC
AGTTCTTCGCCGGCCTGGACCCGACCTCTGCGTTCATCTTCGCCCTGCTGGCATTCGCGGCAGGCTTCATCGTGCGCCCG
TTCGGCGCGCTGGTGTTCGGCCGCCTCGGCGACATGATCGGCCGCAAGTACACCTTCCTGGTGACGATCCTGATCATGGG
CCTGTCGACGTTCATCGTCGGGCTGCTCCCGGGCTACGCCACCATCGGCTGGGCCGCCCCGATCATCCTGATCGCGCTGC
GCATGCTGCAGGGCCTCGCGCTCGGCGGCGAGTACGGCGGTGCCGCCACGTACGTGGCCGAGCATGCGCCGCACGGCAAA
CGCGGTTCCTACACGGCCTGGATCCAGACCACCGCGACGCTGGGCCTGTTCCTGTCGCTGATCGTGATCCTGCTGTGCCG
CGAGCTGACCGGTGCCAACTTCGAAGCCTGGGGCTGGCGTATTCCGTTCCTGGTCTCGATCCTGCTGCTGGCCATGTCGG
TGTACATCCGCCTGTCGATGAGCGAGTCGCCGGCGTTCCAGCGCATGAAGGCCGAGGGCAAGACCTCGAAGGCTCCGCTG
ACCGAAGCGTTCGGCCAGTGGCGCAACCTGAAGATCGTGATCGTGGCGCTGGTGGGCCTGACCGCCGGCCAGGCCGTGGT
CTGGTACACCGGCCAGTTCTACTCGCTGTTCTTCCTGACGCAGGTGCTCAAGGTCGATGCCAAGACGGCCAACCTGCTGA
TCGCCGGCGCGCTGGTGATCGGCACACCGTTCTTCCTGTTCTTCGGCTCGCTGTCGGACAAGATCGGCCGCAAGTGGATC
ATCATGCTGGGCTGCGCGCTCGCCGTGCTGACCTACTTCCCGCTGTTCAAGGCCCTGACGCACTATGCAAACCCGGCGCT
CGAGCGTGCGCAGCAAACCGCGCAGATCGTTGTCACCGCCGACCCGAAGCAGTGCTCGTTCCAGGGCAGCCCGATCGCAC
GTGAAATCGACTTCCGCAGCTCCTGCGATATCGTCAAGCGTACGCTGGCCCAGGCATCGGCAAGCTATGAAGTGGTGCAG
GCTCCGGCCGGCACGGTCGCTTCGGTGAAGATCGGCGACAAGGAGATCAAGGCGTTCGACGCTACCGTCACGGGCGGCCA
CAGCTTCGACGATGCCAGCAAGAAGCAGATCGCCGCGTTCAAGAAGGAGGTCGGCGAGTCCATGGCCGCGGCAGGCTACC
CGACCAAGGCTGATCCGGCGCAGATGAACACCATCATGGTGCTGGTGGTCCTGGTGATTCTGGTGATCTATGTGACGATG
GTGTACGGGCCGATCGCCGCCATGCTGGTGGAAATGTTCCCGACGCGTATCCGCTACTCGTCGATGTCGCTGCCCTACCA
TATCGGCAACGGCTGGTTCGGCGGGCTGCTGCCTACCATCTCGTTCGCGCTGGTCGCCCAGAACGGCAACATCTACTACG
GCCTCTGGTACCCGATCATCATCGCCGCCGTCACCTTCGTGGTCGGTGCCCTGTTCGTGCGTGAAACCAAGGACGTGGAC
ATCTACGCCCACGACTGA

Upstream 100 bases:

>100_bases
CCAGAATCGTTTCAGCTCCGGTGCTGCGGCTCCGCAGCGCGCCATGTCTTGCGGTGCGTCCGCGGCCTCACCGGTGACCC
ATTCAGACGGAGGAGACAAC

Downstream 100 bases:

>100_bases
GCAACAGGTTAAGGCGAGACCGACCCGGTAATCGGTCGCTCCAGCCGGCAGGCCGCAAGGCCTGCCGGTTTTCTTTTCGG
CGCCCTGCAAACATCTTCTC

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 565; Mature: 564

Protein sequence:

>565_residues
MANTQNVAMPGGGAQNAPMTTEEKKVILASSLGTVFEWYDFYLYGSLAAVIAKQFFAGLDPTSAFIFALLAFAAGFIVRP
FGALVFGRLGDMIGRKYTFLVTILIMGLSTFIVGLLPGYATIGWAAPIILIALRMLQGLALGGEYGGAATYVAEHAPHGK
RGSYTAWIQTTATLGLFLSLIVILLCRELTGANFEAWGWRIPFLVSILLLAMSVYIRLSMSESPAFQRMKAEGKTSKAPL
TEAFGQWRNLKIVIVALVGLTAGQAVVWYTGQFYSLFFLTQVLKVDAKTANLLIAGALVIGTPFFLFFGSLSDKIGRKWI
IMLGCALAVLTYFPLFKALTHYANPALERAQQTAQIVVTADPKQCSFQGSPIAREIDFRSSCDIVKRTLAQASASYEVVQ
APAGTVASVKIGDKEIKAFDATVTGGHSFDDASKKQIAAFKKEVGESMAAAGYPTKADPAQMNTIMVLVVLVILVIYVTM
VYGPIAAMLVEMFPTRIRYSSMSLPYHIGNGWFGGLLPTISFALVAQNGNIYYGLWYPIIIAAVTFVVGALFVRETKDVD
IYAHD

Sequences:

>Translated_565_residues
MANTQNVAMPGGGAQNAPMTTEEKKVILASSLGTVFEWYDFYLYGSLAAVIAKQFFAGLDPTSAFIFALLAFAAGFIVRP
FGALVFGRLGDMIGRKYTFLVTILIMGLSTFIVGLLPGYATIGWAAPIILIALRMLQGLALGGEYGGAATYVAEHAPHGK
RGSYTAWIQTTATLGLFLSLIVILLCRELTGANFEAWGWRIPFLVSILLLAMSVYIRLSMSESPAFQRMKAEGKTSKAPL
TEAFGQWRNLKIVIVALVGLTAGQAVVWYTGQFYSLFFLTQVLKVDAKTANLLIAGALVIGTPFFLFFGSLSDKIGRKWI
IMLGCALAVLTYFPLFKALTHYANPALERAQQTAQIVVTADPKQCSFQGSPIAREIDFRSSCDIVKRTLAQASASYEVVQ
APAGTVASVKIGDKEIKAFDATVTGGHSFDDASKKQIAAFKKEVGESMAAAGYPTKADPAQMNTIMVLVVLVILVIYVTM
VYGPIAAMLVEMFPTRIRYSSMSLPYHIGNGWFGGLLPTISFALVAQNGNIYYGLWYPIIIAAVTFVVGALFVRETKDVD
IYAHD
>Mature_564_residues
ANTQNVAMPGGGAQNAPMTTEEKKVILASSLGTVFEWYDFYLYGSLAAVIAKQFFAGLDPTSAFIFALLAFAAGFIVRPF
GALVFGRLGDMIGRKYTFLVTILIMGLSTFIVGLLPGYATIGWAAPIILIALRMLQGLALGGEYGGAATYVAEHAPHGKR
GSYTAWIQTTATLGLFLSLIVILLCRELTGANFEAWGWRIPFLVSILLLAMSVYIRLSMSESPAFQRMKAEGKTSKAPLT
EAFGQWRNLKIVIVALVGLTAGQAVVWYTGQFYSLFFLTQVLKVDAKTANLLIAGALVIGTPFFLFFGSLSDKIGRKWII
MLGCALAVLTYFPLFKALTHYANPALERAQQTAQIVVTADPKQCSFQGSPIAREIDFRSSCDIVKRTLAQASASYEVVQA
PAGTVASVKIGDKEIKAFDATVTGGHSFDDASKKQIAAFKKEVGESMAAAGYPTKADPAQMNTIMVLVVLVILVIYVTMV
YGPIAAMLVEMFPTRIRYSSMSLPYHIGNGWFGGLLPTISFALVAQNGNIYYGLWYPIIIAAVTFVVGALFVRETKDVDI
YAHD

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=325, Percent_Identity=37.8461538461538, Blast_Score=202, Evalue=6e-53,
Organism=Escherichia coli, GI1788292, Length=326, Percent_Identity=35.2760736196319, Blast_Score=192, Evalue=7e-50,
Organism=Escherichia coli, GI1789941, Length=222, Percent_Identity=44.1441441441441, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1788942, Length=336, Percent_Identity=32.1428571428571, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI87082231, Length=230, Percent_Identity=27.3913043478261, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87082404, Length=316, Percent_Identity=24.0506329113924, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 61156; Mature: 61024

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANTQNVAMPGGGAQNAPMTTEEKKVILASSLGTVFEWYDFYLYGSLAAVIAKQFFAGLD
CCCCCCEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PTSAFIFALLAFAAGFIVRPFGALVFGRLGDMIGRKYTFLVTILIMGLSTFIVGLLPGYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHH
TIGWAAPIILIALRMLQGLALGGEYGGAATYVAEHAPHGKRGSYTAWIQTTATLGLFLSL
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHH
IVILLCRELTGANFEAWGWRIPFLVSILLLAMSVYIRLSMSESPAFQRMKAEGKTSKAPL
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCCCCCCH
TEAFGQWRNLKIVIVALVGLTAGQAVVWYTGQFYSLFFLTQVLKVDAKTANLLIAGALVI
HHHHHCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
GTPFFLFFGSLSDKIGRKWIIMLGCALAVLTYFPLFKALTHYANPALERAQQTAQIVVTA
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCEEEEEEE
DPKQCSFQGSPIAREIDFRSSCDIVKRTLAQASASYEVVQAPAGTVASVKIGDKEIKAFD
CCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCEEECCCCCEEEEEECCHHHHHEE
ATVTGGHSFDDASKKQIAAFKKEVGESMAAAGYPTKADPAQMNTIMVLVVLVILVIYVTM
CEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VYGPIAAMLVEMFPTRIRYSSMSLPYHIGNGWFGGLLPTISFALVAQNGNIYYGLWYPII
HHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHEEEEEECCCEEEEHHHHHH
IAAVTFVVGALFVRETKDVDIYAHD
HHHHHHHHHHHHHHCCCCEEEEECC
>Mature Secondary Structure 
ANTQNVAMPGGGAQNAPMTTEEKKVILASSLGTVFEWYDFYLYGSLAAVIAKQFFAGLD
CCCCCEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PTSAFIFALLAFAAGFIVRPFGALVFGRLGDMIGRKYTFLVTILIMGLSTFIVGLLPGYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHH
TIGWAAPIILIALRMLQGLALGGEYGGAATYVAEHAPHGKRGSYTAWIQTTATLGLFLSL
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHH
IVILLCRELTGANFEAWGWRIPFLVSILLLAMSVYIRLSMSESPAFQRMKAEGKTSKAPL
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCCCCCCH
TEAFGQWRNLKIVIVALVGLTAGQAVVWYTGQFYSLFFLTQVLKVDAKTANLLIAGALVI
HHHHHCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
GTPFFLFFGSLSDKIGRKWIIMLGCALAVLTYFPLFKALTHYANPALERAQQTAQIVVTA
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCEEEEEEE
DPKQCSFQGSPIAREIDFRSSCDIVKRTLAQASASYEVVQAPAGTVASVKIGDKEIKAFD
CCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCEEECCCCCEEEEEECCHHHHHEE
ATVTGGHSFDDASKKQIAAFKKEVGESMAAAGYPTKADPAQMNTIMVLVVLVILVIYVTM
CEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VYGPIAAMLVEMFPTRIRYSSMSLPYHIGNGWFGGLLPTISFALVAQNGNIYYGLWYPII
HHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHEEEEEECCCEEEEHHHHHH
IAAVTFVVGALFVRETKDVDIYAHD
HHHHHHHHHHHHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]