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The map label for this gene is miaB [H]

Identifier: 73540220

GI number: 73540220

Start: 558693

End: 560045

Strand: Reverse

Name: miaB [H]

Synonym: Reut_A0514

Alternate gene names: 73540220

Gene position: 560045-558693 (Counterclockwise)

Preceding gene: 73540221

Following gene: 73540219

Centisome position: 14.71

GC content: 64.01

Gene sequence:

>1353_bases
ATGAAGAAAGTATTTGTGAAGACGTACGGCTGTCAGATGAACGAGTATGACTCGGACAAGATGGTCGACGTCCTGAACGC
CAGCCAGGGGCTGGAACCCACCGACAACGTCGAAGACGCCGACGTGATCCTGTTCAACACCTGCTCGGTGCGCGAGAAAG
CGCAGGAAAAGGTGTTCTCCGAACTGGGCCGCATGAAGGCGCTCAAGGCCGTGAAGCCCGATCTCGTGATCGGCGTGGGC
GGCTGCGTGGCAAGCCAGGAAGGCGCGAGCATCGTCTCGCGCGCGCCGTATGTCGATGTCGTGTTCGGTCCGCAAACCCT
GCACCGCCTGCCCGACCTGATCGCGCGCCGCCAGCGCACCGGCCAGTCGCAGGTCGATATCTCGTTCCCCGAGATCGAGA
AGTTCGACCACCTGCCGCCGGCACGCGTGGAAGGCCCGAGCGCCTTTGTCTCGATCATGGAAGGCTGCTCGAAGTACTGC
AGCTACTGTGTGGTGCCCTACACGCGCGGCGAAGAAGTCTCGCGTCCGTTCGAAGACGTGCTGGCCGAAGTGGCCGGACT
CGCGGAACAGGGTGTGCGTGAAGTCACGCTGCTGGGCCAGAACGTCAACGCGTATCGCGGCGCCATGGGCGGCACCAGCG
AGATCGCCGACTTCGCGCTGCTGATCGAGTACGTGGCAGAGATCCCCGGCATCGAGCGCATCCGCTACACCACGAGCCAC
CCGAAGGAATTCACGAGCCGCCTGGTCGAGCTGTACGGCCGCTGCGACAAGCTCGTCAACCACCTGCACCTGCCGGTGCA
GCACGCGTCTGACCGCGTGCTGATGGCGATGAAGCGTGGCTACAGCGTGCTCGAATACAAGAGCATCATCCGCCGGCTGC
GCATGCTGCGTCCCGACATGTCGATGTCGTCGGACTTCATCGTCGGCTTCCCCGGCGAGACCGATGCGGATTTCGACAAG
CTGATGGCGCTGATCGAGGAAGTCGGCTACGACACCTCGTTCTCGTTCATCTTCAGCCCGCGTCCCGGCACGCCGGCGGC
CAACCTGCACGACGACACGCCGCATGAAGTGAAGCTGCGCCGCCTGCAGCATCTGCAGGCCACCATCGAAGAGAACGTGC
AGCGCATCAGCCGCAATATGGTCGGCTCGGTGCAGCGCATCCTCGTCGAAGGCCCGTCGCGCAAGGACCCGACCGAACTG
CACGGCCGCACCGAGAACAACCGCGTGGTCAACTTCGCGCTGCCGGGCGTGCCGCAGGCCCAGCGCGACCGCATGATCGG
CCAGATGGTCGATGTGTCGATCACTCAGGCGTTCCCGCATTCGCTGCGCGGCGAGATCGTGGTGCGGCAATGA

Upstream 100 bases:

>100_bases
GAAGGCCAAGTCCTGATCCCCGGGAAGTACGGAGGCGTTCGCGCCATCCGTGTTGTTGCAACACCCTGATGCCCCCAGGT
GAACCACTGAGACCGGCACC

Downstream 100 bases:

>100_bases
GCGAAGGCATCCCACGCGCCAAGATGAAGATCGCCACCGCCGAATTCGTTGCCCCGCGCGACGACAACACGCGGCTGCAG
AACCTGTGCGGTCCGCTCGA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MKKVFVKTYGCQMNEYDSDKMVDVLNASQGLEPTDNVEDADVILFNTCSVREKAQEKVFSELGRMKALKAVKPDLVIGVG
GCVASQEGASIVSRAPYVDVVFGPQTLHRLPDLIARRQRTGQSQVDISFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYC
SYCVVPYTRGEEVSRPFEDVLAEVAGLAEQGVREVTLLGQNVNAYRGAMGGTSEIADFALLIEYVAEIPGIERIRYTTSH
PKEFTSRLVELYGRCDKLVNHLHLPVQHASDRVLMAMKRGYSVLEYKSIIRRLRMLRPDMSMSSDFIVGFPGETDADFDK
LMALIEEVGYDTSFSFIFSPRPGTPAANLHDDTPHEVKLRRLQHLQATIEENVQRISRNMVGSVQRILVEGPSRKDPTEL
HGRTENNRVVNFALPGVPQAQRDRMIGQMVDVSITQAFPHSLRGEIVVRQ

Sequences:

>Translated_450_residues
MKKVFVKTYGCQMNEYDSDKMVDVLNASQGLEPTDNVEDADVILFNTCSVREKAQEKVFSELGRMKALKAVKPDLVIGVG
GCVASQEGASIVSRAPYVDVVFGPQTLHRLPDLIARRQRTGQSQVDISFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYC
SYCVVPYTRGEEVSRPFEDVLAEVAGLAEQGVREVTLLGQNVNAYRGAMGGTSEIADFALLIEYVAEIPGIERIRYTTSH
PKEFTSRLVELYGRCDKLVNHLHLPVQHASDRVLMAMKRGYSVLEYKSIIRRLRMLRPDMSMSSDFIVGFPGETDADFDK
LMALIEEVGYDTSFSFIFSPRPGTPAANLHDDTPHEVKLRRLQHLQATIEENVQRISRNMVGSVQRILVEGPSRKDPTEL
HGRTENNRVVNFALPGVPQAQRDRMIGQMVDVSITQAFPHSLRGEIVVRQ
>Mature_450_residues
MKKVFVKTYGCQMNEYDSDKMVDVLNASQGLEPTDNVEDADVILFNTCSVREKAQEKVFSELGRMKALKAVKPDLVIGVG
GCVASQEGASIVSRAPYVDVVFGPQTLHRLPDLIARRQRTGQSQVDISFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYC
SYCVVPYTRGEEVSRPFEDVLAEVAGLAEQGVREVTLLGQNVNAYRGAMGGTSEIADFALLIEYVAEIPGIERIRYTTSH
PKEFTSRLVELYGRCDKLVNHLHLPVQHASDRVLMAMKRGYSVLEYKSIIRRLRMLRPDMSMSSDFIVGFPGETDADFDK
LMALIEEVGYDTSFSFIFSPRPGTPAANLHDDTPHEVKLRRLQHLQATIEENVQRISRNMVGSVQRILVEGPSRKDPTEL
HGRTENNRVVNFALPGVPQAQRDRMIGQMVDVSITQAFPHSLRGEIVVRQ

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=485, Percent_Identity=38.7628865979381, Blast_Score=316, Evalue=2e-86,
Organism=Homo sapiens, GI28872782, Length=485, Percent_Identity=38.7628865979381, Blast_Score=316, Evalue=3e-86,
Organism=Homo sapiens, GI93277076, Length=461, Percent_Identity=28.6334056399132, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1786882, Length=447, Percent_Identity=62.4161073825503, Blast_Score=558, Evalue=1e-160,
Organism=Escherichia coli, GI1787057, Length=427, Percent_Identity=27.4004683840749, Blast_Score=155, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17553146, Length=466, Percent_Identity=34.3347639484979, Blast_Score=266, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI71996771, Length=313, Percent_Identity=29.073482428115, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI21356207, Length=479, Percent_Identity=34.6555323590814, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI19922432, Length=463, Percent_Identity=30.0215982721382, Blast_Score=151, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 50290; Mature: 50290

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVFVKTYGCQMNEYDSDKMVDVLNASQGLEPTDNVEDADVILFNTCSVREKAQEKVFS
CCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHH
ELGRMKALKAVKPDLVIGVGGCVASQEGASIVSRAPYVDVVFGPQTLHRLPDLIARRQRT
HHHHHHHHHHCCCCEEEECCCHHCCCCCHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHC
GQSQVDISFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGEEVSRPFEDV
CCCCEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEECCCCCCCHHCCCHHHH
LAEVAGLAEQGVREVTLLGQNVNAYRGAMGGTSEIADFALLIEYVAEIPGIERIRYTTSH
HHHHHHHHHHCCCEEEHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCC
PKEFTSRLVELYGRCDKLVNHLHLPVQHASDRVLMAMKRGYSVLEYKSIIRRLRMLRPDM
HHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
SMSSDFIVGFPGETDADFDKLMALIEEVGYDTSFSFIFSPRPGTPAANLHDDTPHEVKLR
CCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHH
RLQHLQATIEENVQRISRNMVGSVQRILVEGPSRKDPTELHGRTENNRVVNFALPGVPQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEEEECCCCCHH
QRDRMIGQMVDVSITQAFPHSLRGEIVVRQ
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEC
>Mature Secondary Structure
MKKVFVKTYGCQMNEYDSDKMVDVLNASQGLEPTDNVEDADVILFNTCSVREKAQEKVFS
CCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHH
ELGRMKALKAVKPDLVIGVGGCVASQEGASIVSRAPYVDVVFGPQTLHRLPDLIARRQRT
HHHHHHHHHHCCCCEEEECCCHHCCCCCHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHC
GQSQVDISFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGEEVSRPFEDV
CCCCEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEECCCCCCCHHCCCHHHH
LAEVAGLAEQGVREVTLLGQNVNAYRGAMGGTSEIADFALLIEYVAEIPGIERIRYTTSH
HHHHHHHHHHCCCEEEHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCC
PKEFTSRLVELYGRCDKLVNHLHLPVQHASDRVLMAMKRGYSVLEYKSIIRRLRMLRPDM
HHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
SMSSDFIVGFPGETDADFDKLMALIEEVGYDTSFSFIFSPRPGTPAANLHDDTPHEVKLR
CCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHH
RLQHLQATIEENVQRISRNMVGSVQRILVEGPSRKDPTELHGRTENNRVVNFALPGVPQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEEEECCCCCHH
QRDRMIGQMVDVSITQAFPHSLRGEIVVRQ
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA