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The map label for this gene is yafJ [C]

Identifier: 73540167

GI number: 73540167

Start: 506908

End: 507738

Strand: Reverse

Name: yafJ [C]

Synonym: Reut_A0461

Alternate gene names: 73540167

Gene position: 507738-506908 (Counterclockwise)

Preceding gene: 73540172

Following gene: 73540160

Centisome position: 13.34

GC content: 63.54

Gene sequence:

>831_bases
ATGTGCCAGCTGCTAGGCATGAATTGCGCCACGCCAACCGACGTGACGTTTTCCTTCACCGGCTTCGCCGCCCGCGGCGG
GCTGACCGACCACCACGCCGACGGCTTTGGCGTGGCTTTCTTCGAAGACAAGGCCTGCCGGTTGTTCATCGACAACCAGT
CGGCGGGCACGTCGCCCGTGGCAGACCTGATCAAGCGCTATCCGATCAAGTCGAAGAACGTCATTTCGCATATCCGCAAG
GCCACGCAAGGCACGGTCCTGCTCGAGAACTGTCACCCGTTCATGCGCGAACTGTGGGGACGGCACTGGATCTTCGCCCA
CAACGGCGACCTGCTGAACTTCACGCCATTCCTGTCCGGCGTCTACCAGCCCGTGGGCGACACCGACAGCGAACTCGCGT
TCTGCACGCTGATGCAGGGGCTGCGCAAGCGCTTCCCCGGTTCGCAGCCGCCGCTGAACGAGCTGTGCCATGCGCTAGCG
GACATCACGCGCGAAATCACCACGCACGGTGTGTTCAACTTCCTGCTGTGCAACGGGCAGGCCCTGTTCGCGCACTGCTC
GACGCGGCTGTACTACATCGTGCGGCAATGGCCGTTCTCGACCGCGCACCTGATCGACGCCGACCTGTCGATCGACTTCG
CCCAGGTCACCACGCCGGATGACCGGGTCGCGGTGATCGCCACGCAGCCGCTTACCGACAACGAGACCTGGACGCCCTTC
GAGCCGGGCGAGTTGATCATGTTCATCGACGGACGGCCGGAGATGACGCTGTCAGTGCCAATTCCGCCGGAAGTGCAGGC
GAAGAACGCAGCCAACACGGCCTGTACCTGA

Upstream 100 bases:

>100_bases
AGGCGCCCGGACGCGTGCGCTTGGCAGCGGCCCGGGGGCGCGGGATAATGTCTGCATTCTATTGCAAAGCGGCAGCCCCC
TGCCGATCCGGTGCCCCGCC

Downstream 100 bases:

>100_bases
ATCCAGGCGGGGCCGGAAAGAAAAAGGGACGCCTCGGCGTCCCTTATTTCTCGCAGCGGGGCGCAAGACCTCAGTGCTGC
AGAATCTTCGACAGGAACTG

Product: glutamine amidotransferase, class-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MCQLLGMNCATPTDVTFSFTGFAARGGLTDHHADGFGVAFFEDKACRLFIDNQSAGTSPVADLIKRYPIKSKNVISHIRK
ATQGTVLLENCHPFMRELWGRHWIFAHNGDLLNFTPFLSGVYQPVGDTDSELAFCTLMQGLRKRFPGSQPPLNELCHALA
DITREITTHGVFNFLLCNGQALFAHCSTRLYYIVRQWPFSTAHLIDADLSIDFAQVTTPDDRVAVIATQPLTDNETWTPF
EPGELIMFIDGRPEMTLSVPIPPEVQAKNAANTACT

Sequences:

>Translated_276_residues
MCQLLGMNCATPTDVTFSFTGFAARGGLTDHHADGFGVAFFEDKACRLFIDNQSAGTSPVADLIKRYPIKSKNVISHIRK
ATQGTVLLENCHPFMRELWGRHWIFAHNGDLLNFTPFLSGVYQPVGDTDSELAFCTLMQGLRKRFPGSQPPLNELCHALA
DITREITTHGVFNFLLCNGQALFAHCSTRLYYIVRQWPFSTAHLIDADLSIDFAQVTTPDDRVAVIATQPLTDNETWTPF
EPGELIMFIDGRPEMTLSVPIPPEVQAKNAANTACT
>Mature_276_residues
MCQLLGMNCATPTDVTFSFTGFAARGGLTDHHADGFGVAFFEDKACRLFIDNQSAGTSPVADLIKRYPIKSKNVISHIRK
ATQGTVLLENCHPFMRELWGRHWIFAHNGDLLNFTPFLSGVYQPVGDTDSELAFCTLMQGLRKRFPGSQPPLNELCHALA
DITREITTHGVFNFLLCNGQALFAHCSTRLYYIVRQWPFSTAHLIDADLSIDFAQVTTPDDRVAVIATQPLTDNETWTPF
EPGELIMFIDGRPEMTLSVPIPPEVQAKNAANTACT

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=251, Percent_Identity=50.199203187251, Blast_Score=270, Evalue=6e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 30610; Mature: 30610

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCQLLGMNCATPTDVTFSFTGFAARGGLTDHHADGFGVAFFEDKACRLFIDNQSAGTSPV
CCEEECCCCCCCCCCEEEEECEEECCCCCCCCCCCEEEEEEECCEEEEEEECCCCCCCHH
ADLIKRYPIKSKNVISHIRKATQGTVLLENCHPFMRELWGRHWIFAHNGDLLNFTPFLSG
HHHHHHCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHCCCEEEEEECCCEEEHHHHHHH
VYQPVGDTDSELAFCTLMQGLRKRFPGSQPPLNELCHALADITREITTHGVFNFLLCNGQ
HHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCC
ALFAHCSTRLYYIVRQWPFSTAHLIDADLSIDFAQVTTPDDRVAVIATQPLTDNETWTPF
EEEEHHCCEEEEEEEECCCCCEEEEECCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCC
EPGELIMFIDGRPEMTLSVPIPPEVQAKNAANTACT
CCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MCQLLGMNCATPTDVTFSFTGFAARGGLTDHHADGFGVAFFEDKACRLFIDNQSAGTSPV
CCEEECCCCCCCCCCEEEEECEEECCCCCCCCCCCEEEEEEECCEEEEEEECCCCCCCHH
ADLIKRYPIKSKNVISHIRKATQGTVLLENCHPFMRELWGRHWIFAHNGDLLNFTPFLSG
HHHHHHCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHCCCEEEEEECCCEEEHHHHHHH
VYQPVGDTDSELAFCTLMQGLRKRFPGSQPPLNELCHALADITREITTHGVFNFLLCNGQ
HHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCC
ALFAHCSTRLYYIVRQWPFSTAHLIDADLSIDFAQVTTPDDRVAVIATQPLTDNETWTPF
EEEEHHCCEEEEEEEECCCCCEEEEECCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCC
EPGELIMFIDGRPEMTLSVPIPPEVQAKNAANTACT
CCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]