Click here to switch to the map view.

The map label for this gene is aspS [H]

Identifier: 73540145

GI number: 73540145

Start: 482561

End: 484369

Strand: Direct

Name: aspS [H]

Synonym: Reut_A0439

Alternate gene names: 73540145

Gene position: 482561-484369 (Clockwise)

Preceding gene: 73540144

Following gene: 73540146

Centisome position: 12.68

GC content: 63.46

Gene sequence:

>1809_bases
ATGTCCTCCATGCGTACTCACTACTGCGGTCTGGTGACCGAACAACTCTCGGGCCAGGAAGTGGCCCTGACCGGCTGGGT
TCAGCGCCGCCGCGACCATGGCGGCGTGATCTTCATCGACCTGCGCGACCGTGAAGGCCTGGTGCAGGTGGTGTGCGATC
CGGACCGCCCGGAAATGTTCAAGGCCGCCGAAGAGATCCGCAACGAGTACTGCATCCGCATTACCGGCAAGGTGCGTCCG
CGCCCGGCCGGCACCGAGAACACCAACCTGACCTCGGGCAAGATCGAAGTGCTGTGCTACGAGCTGACCGTGCTGAACCC
CTCGGTCACGCCCCCGTTCCAGCTCGACGACGACAACCTGTCGGAAACCACGCGCCTCACGCATCGCGTGCTGGACCTGC
GCCGCCCGCAGATGCAGTACAACCTGCGCCTGCGCTACAAGGTCGCGATGGAAGTGCGCAAGTACCTGGACGCGCAAGGC
TTCATCGACATCGAAACCCCGATGCTCGGCAAGAGCACGCCGGAAGGCGCCCGCGACTATCTGGTGCCGTCGCGCGTGAA
CCCGGGTCACTTCTTCGCGCTGCCGCAATCGCCGCAGATCTTCAAGCAGATGCTGATGGTCTCGGGCTTTGACCGCTACT
ACCAGATCACCAAGTGCTTTCGCGACGAAGACCTGCGCGCTGACCGCCAGCCCGAATTCACGCAGATCGACTGCGAGACC
TCGTTCCTGACCGAGCAGGAAATCCGTGACCTGTTCGAAGACATGATGCGCACGGTGTTCAAGAACGCCATCGATGTCGA
CCTGGATGCCAAGTTCCCGGTGATGGAGTTCCGCGAAGCCATGGCCCGTTTCGGCTCGGACAAGCCGGACCTGCGCGTGA
AGCTCGAATTCACCGAACTGACCGACGTGATGAAGGACGTCGACTTCAAGGTGTTCTCGGGTCCCGCCAACAGCGACAAC
GGCCGCGTGGTCGGCCTGCGCGTGCCGGGCGGCGGCGCGATCTCGCGCGGCGAGATCGATGCCTACACGCAATTTGTCGG
TATCTACGGCGCCAAGGGCCTGGCCTGGGTCAAGGTCAACGAAGTGGCCAAGGGCCGTGACGGCCTGCAGTCGCCGATCG
TCAAGAACCTGCACGACGCGGCAATTACCGAAATCCTGAAGCGCACCGGCGCCCAGGACGGCGACATCATCTTCTTCGGC
GCCGACAAGGCCAAGGTCGTCAACGACGCCATCGGCGCGCTGCGCCTGAAGATCGGCCACTCGGAATTCGGCAAGGCCAG
CGGCCTGTTCGAAGACGTGTGGAAGCCGCTGTGGGTGGTCGATTTCCCGATGTTCGAGTACGACGAGGAAGATGCCCGCT
GGGTGGCCATGCACCACCCGTTCACGAGCCCGAAGGACGAGCACATGCAGTACCTGGAAACCGATCCGGGCAAGTGCATC
GCCAAGGCCTACGACATGGTCCTGAACGGCTGGGAAATGGGTGGCGGCTCGGTCCGTATCTACCGTTCGGATATCCAGAG
CAAGGTGTTCCGTGCGCTGAAGATCAACGACGAAGAGGCGCGTGCCAAGTTCGGTTACCTGCTTGACGCGCTGCAATACG
GCGCGCCCCCGCACGGTGGCCTGGCCTTTGGCCTGGACCGTATCGTCACGATGATGGCCGGAGCCGATTCGATCCGCGAC
GTGATCGCCTTCCCGAAGACCCAGCGCGCCCAGGATCTGCTGACGCAGGCCCCGAGCGCCGTCGACGAGAAGCAACTGCG
CGAGCTGCATATCCGCCTGCGCGCCACCGAGCCGAAGACCACGGTCTGA

Upstream 100 bases:

>100_bases
GGCGCCGGCCGCGAGGCAGAGGTCGAGACGGCCGATCCTTCGCATACCAATTGATCACGGGCCGCCTGGCGGGCGGTCAC
GTATTACCGGGAAATATCCT

Downstream 100 bases:

>100_bases
GCCTGACGGCCCTGGCTGAAGAAAAACCGCGCCGCGTGCGCGGTTTTTTCGTTTATGGATGCCTGCGGACGTTTGCGGAC
GCAAGTGATCCGTGGCTGGC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 602; Mature: 601

Protein sequence:

>602_residues
MSSMRTHYCGLVTEQLSGQEVALTGWVQRRRDHGGVIFIDLRDREGLVQVVCDPDRPEMFKAAEEIRNEYCIRITGKVRP
RPAGTENTNLTSGKIEVLCYELTVLNPSVTPPFQLDDDNLSETTRLTHRVLDLRRPQMQYNLRLRYKVAMEVRKYLDAQG
FIDIETPMLGKSTPEGARDYLVPSRVNPGHFFALPQSPQIFKQMLMVSGFDRYYQITKCFRDEDLRADRQPEFTQIDCET
SFLTEQEIRDLFEDMMRTVFKNAIDVDLDAKFPVMEFREAMARFGSDKPDLRVKLEFTELTDVMKDVDFKVFSGPANSDN
GRVVGLRVPGGGAISRGEIDAYTQFVGIYGAKGLAWVKVNEVAKGRDGLQSPIVKNLHDAAITEILKRTGAQDGDIIFFG
ADKAKVVNDAIGALRLKIGHSEFGKASGLFEDVWKPLWVVDFPMFEYDEEDARWVAMHHPFTSPKDEHMQYLETDPGKCI
AKAYDMVLNGWEMGGGSVRIYRSDIQSKVFRALKINDEEARAKFGYLLDALQYGAPPHGGLAFGLDRIVTMMAGADSIRD
VIAFPKTQRAQDLLTQAPSAVDEKQLRELHIRLRATEPKTTV

Sequences:

>Translated_602_residues
MSSMRTHYCGLVTEQLSGQEVALTGWVQRRRDHGGVIFIDLRDREGLVQVVCDPDRPEMFKAAEEIRNEYCIRITGKVRP
RPAGTENTNLTSGKIEVLCYELTVLNPSVTPPFQLDDDNLSETTRLTHRVLDLRRPQMQYNLRLRYKVAMEVRKYLDAQG
FIDIETPMLGKSTPEGARDYLVPSRVNPGHFFALPQSPQIFKQMLMVSGFDRYYQITKCFRDEDLRADRQPEFTQIDCET
SFLTEQEIRDLFEDMMRTVFKNAIDVDLDAKFPVMEFREAMARFGSDKPDLRVKLEFTELTDVMKDVDFKVFSGPANSDN
GRVVGLRVPGGGAISRGEIDAYTQFVGIYGAKGLAWVKVNEVAKGRDGLQSPIVKNLHDAAITEILKRTGAQDGDIIFFG
ADKAKVVNDAIGALRLKIGHSEFGKASGLFEDVWKPLWVVDFPMFEYDEEDARWVAMHHPFTSPKDEHMQYLETDPGKCI
AKAYDMVLNGWEMGGGSVRIYRSDIQSKVFRALKINDEEARAKFGYLLDALQYGAPPHGGLAFGLDRIVTMMAGADSIRD
VIAFPKTQRAQDLLTQAPSAVDEKQLRELHIRLRATEPKTTV
>Mature_601_residues
SSMRTHYCGLVTEQLSGQEVALTGWVQRRRDHGGVIFIDLRDREGLVQVVCDPDRPEMFKAAEEIRNEYCIRITGKVRPR
PAGTENTNLTSGKIEVLCYELTVLNPSVTPPFQLDDDNLSETTRLTHRVLDLRRPQMQYNLRLRYKVAMEVRKYLDAQGF
IDIETPMLGKSTPEGARDYLVPSRVNPGHFFALPQSPQIFKQMLMVSGFDRYYQITKCFRDEDLRADRQPEFTQIDCETS
FLTEQEIRDLFEDMMRTVFKNAIDVDLDAKFPVMEFREAMARFGSDKPDLRVKLEFTELTDVMKDVDFKVFSGPANSDNG
RVVGLRVPGGGAISRGEIDAYTQFVGIYGAKGLAWVKVNEVAKGRDGLQSPIVKNLHDAAITEILKRTGAQDGDIIFFGA
DKAKVVNDAIGALRLKIGHSEFGKASGLFEDVWKPLWVVDFPMFEYDEEDARWVAMHHPFTSPKDEHMQYLETDPGKCIA
KAYDMVLNGWEMGGGSVRIYRSDIQSKVFRALKINDEEARAKFGYLLDALQYGAPPHGGLAFGLDRIVTMMAGADSIRDV
IAFPKTQRAQDLLTQAPSAVDEKQLRELHIRLRATEPKTTV

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=599, Percent_Identity=38.7312186978297, Blast_Score=421, Evalue=1e-117,
Organism=Homo sapiens, GI45439306, Length=271, Percent_Identity=28.0442804428044, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI194272210, Length=261, Percent_Identity=26.0536398467433, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI5031815, Length=261, Percent_Identity=26.0536398467433, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI1788173, Length=591, Percent_Identity=57.8680203045685, Blast_Score=691, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=278, Percent_Identity=25.1798561151079, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1790571, Length=286, Percent_Identity=24.1258741258741, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI32566633, Length=606, Percent_Identity=33.993399339934, Blast_Score=320, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI71994340, Length=269, Percent_Identity=22.6765799256506, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17535927, Length=269, Percent_Identity=22.6765799256506, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=269, Percent_Identity=22.6765799256506, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17551876, Length=268, Percent_Identity=25.7462686567164, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325153, Length=638, Percent_Identity=33.5423197492163, Blast_Score=291, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6323011, Length=304, Percent_Identity=26.6447368421053, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6321807, Length=248, Percent_Identity=25.8064516129032, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324256, Length=183, Percent_Identity=27.8688524590164, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320242, Length=266, Percent_Identity=23.6842105263158, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24584738, Length=606, Percent_Identity=36.1386138613861, Blast_Score=356, Evalue=3e-98,
Organism=Drosophila melanogaster, GI17136276, Length=275, Percent_Identity=29.0909090909091, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24640849, Length=259, Percent_Identity=26.2548262548263, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24640851, Length=259, Percent_Identity=26.2548262548263, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 68136; Mature: 68004

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSMRTHYCGLVTEQLSGQEVALTGWVQRRRDHGGVIFIDLRDREGLVQVVCDPDRPEMF
CCCCCHHHHHHHHHHCCCCEEEEHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCHHHH
KAAEEIRNEYCIRITGKVRPRPAGTENTNLTSGKIEVLCYELTVLNPSVTPPFQLDDDNL
HHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCEEECCCCC
SETTRLTHRVLDLRRPQMQYNLRLRYKVAMEVRKYLDAQGFIDIETPMLGKSTPEGARDY
HHHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHC
LVPSRVNPGHFFALPQSPQIFKQMLMVSGFDRYYQITKCFRDEDLRADRQPEFTQIDCET
CCCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCH
SFLTEQEIRDLFEDMMRTVFKNAIDVDLDAKFPVMEFREAMARFGSDKPDLRVKLEFTEL
HHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEHHHH
TDVMKDVDFKVFSGPANSDNGRVVGLRVPGGGAISRGEIDAYTQFVGIYGAKGLAWVKVN
HHHHHHCCCEEEECCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEH
EVAKGRDGLQSPIVKNLHDAAITEILKRTGAQDGDIIFFGADKAKVVNDAIGALRLKIGH
HHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHEEEEECC
SEFGKASGLFEDVWKPLWVVDFPMFEYDEEDARWVAMHHPFTSPKDEHMQYLETDPGKCI
CCCCCCCCCHHHHCCCEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCHHH
AKAYDMVLNGWEMGGGSVRIYRSDIQSKVFRALKINDEEARAKFGYLLDALQYGAPPHGG
HHHHHHHHCCEEECCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC
LAFGLDRIVTMMAGADSIRDVIAFPKTQRAQDLLTQAPSAVDEKQLRELHIRLRATEPKT
HHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHEEEEEEECCCCC
TV
CC
>Mature Secondary Structure 
SSMRTHYCGLVTEQLSGQEVALTGWVQRRRDHGGVIFIDLRDREGLVQVVCDPDRPEMF
CCCCHHHHHHHHHHCCCCEEEEHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCHHHH
KAAEEIRNEYCIRITGKVRPRPAGTENTNLTSGKIEVLCYELTVLNPSVTPPFQLDDDNL
HHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCEEECCCCC
SETTRLTHRVLDLRRPQMQYNLRLRYKVAMEVRKYLDAQGFIDIETPMLGKSTPEGARDY
HHHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHC
LVPSRVNPGHFFALPQSPQIFKQMLMVSGFDRYYQITKCFRDEDLRADRQPEFTQIDCET
CCCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCH
SFLTEQEIRDLFEDMMRTVFKNAIDVDLDAKFPVMEFREAMARFGSDKPDLRVKLEFTEL
HHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEHHHH
TDVMKDVDFKVFSGPANSDNGRVVGLRVPGGGAISRGEIDAYTQFVGIYGAKGLAWVKVN
HHHHHHCCCEEEECCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEH
EVAKGRDGLQSPIVKNLHDAAITEILKRTGAQDGDIIFFGADKAKVVNDAIGALRLKIGH
HHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHEEEEECC
SEFGKASGLFEDVWKPLWVVDFPMFEYDEEDARWVAMHHPFTSPKDEHMQYLETDPGKCI
CCCCCCCCCHHHHCCCEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCHHH
AKAYDMVLNGWEMGGGSVRIYRSDIQSKVFRALKINDEEARAKFGYLLDALQYGAPPHGG
HHHHHHHHCCEEECCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC
LAFGLDRIVTMMAGADSIRDVIAFPKTQRAQDLLTQAPSAVDEKQLRELHIRLRATEPKT
HHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHEEEEEEECCCCC
TV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA