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The map label for this gene is otsA [H]

Identifier: 73540122

GI number: 73540122

Start: 456389

End: 457780

Strand: Direct

Name: otsA [H]

Synonym: Reut_A0416

Alternate gene names: 73540122

Gene position: 456389-457780 (Clockwise)

Preceding gene: 73540121

Following gene: 73540123

Centisome position: 11.99

GC content: 64.87

Gene sequence:

>1392_bases
ATGCCAAGACTAGTAGCGGTATCCAACCGGGTTGCCGATCCGCGCAACGTTGCCGCGGGCGGCCTCGCCGTGGCCTTGTC
CGAAGCGCTGCGCCAGACTGGCGGGATGTGGTTTGGCTGGAGCGGCAAGGCGCTGGAAACGAACCATGGCGGCACGCCTG
GCGAAGGGGAGCTGCACTTGCAGCAAGCGGGCAATGTCACGCTGGCCACTGTGGACATGTGCCGCGAAGACCATGACGCC
TACTACCTCGGCTACAGCAACGGTGTCCTGTGGCCCGCATTCCACTACCGGCTGGATCTGGCGGACTTCGACTCGAACTA
TCTCAACGGCTACCGTCGCGTGAACCAGCTGTTCGCGCGCAAGCTGGCGCCGCTGCTCAAGCCCGATGACATCATCTGGA
TCCACGACTACCACCTGATCCCTCTAGCGGCGGAATTGCGCGCCATCGGCTGCGGACAGCGCATCGGCTTCTTCCTGCAT
ATCCCGCTGCCGCCACCGCTGATCCTGGCCGCCATCCCGCAGCATGACTGGCTCATGCGCGCGCTGTTCGCGTACGACCT
CATCGGCTTCCAGAGCCACACCGACCTGGAGCATTTCTCGCGCTACGTGCAGAACGAAGCGTTCGCCGAGCCAATGGGCG
AACACCGCTATCGCGCGTTCCATCGCACCGTACGTGCCGAGGCGTTCCCGATCGGCATCGATGTGGAGGAGTTTCGGGAA
CTCGGTCGGGGCGCTGAAGCACAGGAGACCTTCGAGATGATGCGCGCGCAGTACGCGAGCCGCAGGCTGCTGCTCGGCAT
CGACCGACTCGATTACTCCAAGGGCCTGCCGCAGCGGCTCAAGGCCTTCTACCGGCTGCTGGCCGAGTATCCGGAGAACC
GCATGAGCGCGACGCTGGTGCAGATCGCGGCGCCGTCGCGCGAATCGGTAGATGCCTATGCCGACCTGCGCAGCGAGATG
GAACGGCTGAGTGGTGCCATCAACGGTGAATTCGGCGAGCTGGACTGGATGCCGGTGCGCTATATCCATCGCACCACGGC
ACGCAAGAGGCTGCCGGGCTTGTGCCGTGCGAGCCGCGTAGCGCTCGTGACACCGCTGCGCGATGGCATGAATCTCGTGG
CGAAGGAATTCATCGCTGCGCAGGATCGTGAAGACCCTGGCGTGCTCGTGCTGTCGCGCTTTGCGGGCGCGGCCGAGCAG
CTCAAGGAAGCGCTGCTGGTGAACCCCTACGACACGCACGCCACCGCGCAGGCGATCCAGCAGGCATTGCACATGCCGCT
GGCCGAGCGGCAGGCGCGGCATGAGAAGCTGCTGGAGCGCATTCGTACGCAGGACGTGCACTGGTGGAGCCAGGAGTTTC
TGCGCGCGCTGACGGAAACGGACGGCGGCTGA

Upstream 100 bases:

>100_bases
CGGGCTGATCAACGGCGCCACGCGCCTGTCGGCGCCGTGGGACAGCGTGATCTGAGCGGAACGGGACGGGACGGGGCATG
ACAAACACGATCAACCATCT

Downstream 100 bases:

>100_bases
AGGCGCTGGGTTTTGCCTGCCGCTGCTGAAGGAGTGTTGCCATGAGCGAGAAACTGGAATCGCAGACTTGCACGCCTTGC
CGTGGGGGTATTCCTCCGCT

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MPRLVAVSNRVADPRNVAAGGLAVALSEALRQTGGMWFGWSGKALETNHGGTPGEGELHLQQAGNVTLATVDMCREDHDA
YYLGYSNGVLWPAFHYRLDLADFDSNYLNGYRRVNQLFARKLAPLLKPDDIIWIHDYHLIPLAAELRAIGCGQRIGFFLH
IPLPPPLILAAIPQHDWLMRALFAYDLIGFQSHTDLEHFSRYVQNEAFAEPMGEHRYRAFHRTVRAEAFPIGIDVEEFRE
LGRGAEAQETFEMMRAQYASRRLLLGIDRLDYSKGLPQRLKAFYRLLAEYPENRMSATLVQIAAPSRESVDAYADLRSEM
ERLSGAINGEFGELDWMPVRYIHRTTARKRLPGLCRASRVALVTPLRDGMNLVAKEFIAAQDREDPGVLVLSRFAGAAEQ
LKEALLVNPYDTHATAQAIQQALHMPLAERQARHEKLLERIRTQDVHWWSQEFLRALTETDGG

Sequences:

>Translated_463_residues
MPRLVAVSNRVADPRNVAAGGLAVALSEALRQTGGMWFGWSGKALETNHGGTPGEGELHLQQAGNVTLATVDMCREDHDA
YYLGYSNGVLWPAFHYRLDLADFDSNYLNGYRRVNQLFARKLAPLLKPDDIIWIHDYHLIPLAAELRAIGCGQRIGFFLH
IPLPPPLILAAIPQHDWLMRALFAYDLIGFQSHTDLEHFSRYVQNEAFAEPMGEHRYRAFHRTVRAEAFPIGIDVEEFRE
LGRGAEAQETFEMMRAQYASRRLLLGIDRLDYSKGLPQRLKAFYRLLAEYPENRMSATLVQIAAPSRESVDAYADLRSEM
ERLSGAINGEFGELDWMPVRYIHRTTARKRLPGLCRASRVALVTPLRDGMNLVAKEFIAAQDREDPGVLVLSRFAGAAEQ
LKEALLVNPYDTHATAQAIQQALHMPLAERQARHEKLLERIRTQDVHWWSQEFLRALTETDGG
>Mature_462_residues
PRLVAVSNRVADPRNVAAGGLAVALSEALRQTGGMWFGWSGKALETNHGGTPGEGELHLQQAGNVTLATVDMCREDHDAY
YLGYSNGVLWPAFHYRLDLADFDSNYLNGYRRVNQLFARKLAPLLKPDDIIWIHDYHLIPLAAELRAIGCGQRIGFFLHI
PLPPPLILAAIPQHDWLMRALFAYDLIGFQSHTDLEHFSRYVQNEAFAEPMGEHRYRAFHRTVRAEAFPIGIDVEEFREL
GRGAEAQETFEMMRAQYASRRLLLGIDRLDYSKGLPQRLKAFYRLLAEYPENRMSATLVQIAAPSRESVDAYADLRSEME
RLSGAINGEFGELDWMPVRYIHRTTARKRLPGLCRASRVALVTPLRDGMNLVAKEFIAAQDREDPGVLVLSRFAGAAEQL
KEALLVNPYDTHATAQAIQQALHMPLAERQARHEKLLERIRTQDVHWWSQEFLRALTETDGG

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=461, Percent_Identity=46.6377440347072, Blast_Score=389, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI115533560, Length=425, Percent_Identity=29.6470588235294, Blast_Score=175, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI115533558, Length=425, Percent_Identity=29.6470588235294, Blast_Score=175, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI71987012, Length=434, Percent_Identity=28.8018433179723, Blast_Score=163, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6319602, Length=474, Percent_Identity=35.2320675105485, Blast_Score=284, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6320279, Length=436, Percent_Identity=26.605504587156, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6323537, Length=412, Percent_Identity=26.6990291262136, Blast_Score=180, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6323917, Length=373, Percent_Identity=28.4182305630027, Blast_Score=168, Evalue=2e-42,
Organism=Drosophila melanogaster, GI19920676, Length=495, Percent_Identity=37.1717171717172, Blast_Score=294, Evalue=1e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 52424; Mature: 52293

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLVAVSNRVADPRNVAAGGLAVALSEALRQTGGMWFGWSGKALETNHGGTPGEGELHL
CCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCEEECCCCCCCCCCCEEE
QQAGNVTLATVDMCREDHDAYYLGYSNGVLWPAFHYRLDLADFDSNYLNGYRRVNQLFAR
EECCCEEEEEHHHHHCCCCEEEEEECCCCCCCCHHEEEEHHHCCCHHHHHHHHHHHHHHH
KLAPLLKPDDIIWIHDYHLIPLAAELRAIGCGQRIGFFLHIPLPPPLILAAIPQHDWLMR
HHHHHCCCCCEEEEECCEECHHHHHHHHHCCCCCCCEEEECCCCCCEEEEECCCHHHHHH
ALFAYDLIGFQSHTDLEHFSRYVQNEAFAEPMGEHRYRAFHRTVRAEAFPIGIDVEEFRE
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
LGRGAEAQETFEMMRAQYASRRLLLGIDRLDYSKGLPQRLKAFYRLLAEYPENRMSATLV
HCCCCCHHHHHHHHHHHHHCCHHEEEEHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHH
QIAAPSRESVDAYADLRSEMERLSGAINGEFGELDWMPVRYIHRTTARKRLPGLCRASRV
EEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHCCE
ALVTPLRDGMNLVAKEFIAAQDREDPGVLVLSRFAGAAEQLKEALLVNPYDTHATAQAIQ
EEEECCHHHHHHHHHHHHHHCCCCCCCEEEHHHHCCHHHHHHHHHCCCCCCCHHHHHHHH
QALHMPLAERQARHEKLLERIRTQDVHWWSQEFLRALTETDGG
HHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PRLVAVSNRVADPRNVAAGGLAVALSEALRQTGGMWFGWSGKALETNHGGTPGEGELHL
CCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCEEECCCCCCCCCCCEEE
QQAGNVTLATVDMCREDHDAYYLGYSNGVLWPAFHYRLDLADFDSNYLNGYRRVNQLFAR
EECCCEEEEEHHHHHCCCCEEEEEECCCCCCCCHHEEEEHHHCCCHHHHHHHHHHHHHHH
KLAPLLKPDDIIWIHDYHLIPLAAELRAIGCGQRIGFFLHIPLPPPLILAAIPQHDWLMR
HHHHHCCCCCEEEEECCEECHHHHHHHHHCCCCCCCEEEECCCCCCEEEEECCCHHHHHH
ALFAYDLIGFQSHTDLEHFSRYVQNEAFAEPMGEHRYRAFHRTVRAEAFPIGIDVEEFRE
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
LGRGAEAQETFEMMRAQYASRRLLLGIDRLDYSKGLPQRLKAFYRLLAEYPENRMSATLV
HCCCCCHHHHHHHHHHHHHCCHHEEEEHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHH
QIAAPSRESVDAYADLRSEMERLSGAINGEFGELDWMPVRYIHRTTARKRLPGLCRASRV
EEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHCCE
ALVTPLRDGMNLVAKEFIAAQDREDPGVLVLSRFAGAAEQLKEALLVNPYDTHATAQAIQ
EEEECCHHHHHHHHHHHHHHCCCCCCCEEEHHHHCCHHHHHHHHHCCCCCCCHHHHHHHH
QALHMPLAERQARHEKLLERIRTQDVHWWSQEFLRALTETDGG
HHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA