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The map label for this gene is rpoN [H]

Identifier: 73540061

GI number: 73540061

Start: 384623

End: 386104

Strand: Reverse

Name: rpoN [H]

Synonym: Reut_A0355

Alternate gene names: 73540061

Gene position: 386104-384623 (Counterclockwise)

Preceding gene: 73540062

Following gene: 73540060

Centisome position: 10.14

GC content: 60.53

Gene sequence:

>1482_bases
ATGAAACCGTCGCTCCAGCTCCGCCTTTCGCAGCATCTGGCACTCACGCCGCAACTGCAGCAGTCGATCCGACTGCTGCA
ACTCTCAACGCTGGAACTGCAACAGGAAGTCGAGCAGGCGCTCACGGAGAACCCGCTGCTGGAGCGCGAAAACGACTGGA
TCGAAAGTCCGCTGCGCGTAGCGGCAGATGGCTCGGTCAACCTGCAAAGTGCCCCGGCTCCCGCTCCTGCCGAACCGCAG
GGCAATGGCGAAGCGAATGCCGACAGCGCACGCGGCAGCGATGACGAGAACTACACCGACAGCGGCGGTGACGACTACGG
CAGCGACTGGAGCCTCGACGATTTCGCGCGGCGCCCCCAGGGCGAGGACGACGAAAAGACGCCCATGCAGCTGCGCGATG
CCGAGCCCACGCTGCGCGAACATCTGATGGAACAGCTCACGCCGCTGAAGATTTCGCTGCGCGACAAGGGGCTGGCGATC
TTCCTGATCGAATCGCTCGATGACGACGGCTACCTGAGCACGTCGCTCGACGAGATCTGCTCGGAACTGCCCGAGGAACT
CGAATTCGAAGTCGACGAAGTCCAGGCCATGCTCACGCTGCTGCAGAGCTTCGACCCGGCGGGCGTGGGCGCGCGCAATG
CAGCCGAATGCCTGAGCCTGCAATTGCGCCGGCTGCAGCACCCGCAGCGCGAACTGGCGCTGTCGATCGTCCAGCATCAT
CTTGAATTGCTTGCGGTGCGCGACTACACGCGCCTGAAAAAAGCGCTGCAGGTGGACGAAGCGGCGCTAAAAGCCGCGCA
TGAACTGATCCGTTCGCTCGCGCCGTATCCCGGGCATGCGTATAGCCGGCCTGAAGCGGATTTCGTAGTGCCCGACGTGT
TCGTGCGCAAGAGCGGCAGCAGCTGGATTGCACAGCTGAACCCGGATGTGATGCCCCGCCTGCGCATCAACGACATGTAC
GCGCAGATCCTGCGCGGAGCGAAGGGGGAATCCGGCGCTGCCGGGCTGCAGCAAAAGCTGCAGGAGGCACGTTGGCTGAT
CAAAAATATTCAGCAAAGGTTCGACACAATCCTGCGAGTTTCACAGGCAATTGTCGAACGTCAGAAGAACTTTTTCACGC
ACGGCGAAATCGCGATGCGCCCCTTGGTTTTGCGGGAGATTGCCGATACACTGGGATTACACGAGTCAACCATATCCCGG
GTGACGACCAATAAATACATGGCAACGCCGATGGGTACTTTCGAACTGAAGTACTTCTTCGGTAGCCACGTGTCCACCGA
AACCGGTGGCGCGGCTTCATCAACGGCGATCCGCGCCTTGATCAAGCAACTTATAGGAGCCGAAGACCCGAGGAGTCCTC
TTTCCGACAGCCGAATTGCCGAATTGCTGGGTGAACAAGGCTTCGTTGTCGCGCGCCGTACCGTCGCCAAGTATCGCGAA
GCCCTGAAGATCCCCGCTGTCAATCTCCGCAAGACTTTGTAG

Upstream 100 bases:

>100_bases
GCGGGGGTGGCACGCGGCCACCGGGAAAGAGACGATCAGGCGACACATGCGATAGCAATTTCCGTTCCAACATCGGAAAG
ATGCGCATAAAATAGGTCGC

Downstream 100 bases:

>100_bases
CCGAGTTGCACCGCCTGCCTGGTGCAGCTCTTTCAGAAGGAGAAGCGCTATGAACTTCAAGATCAGTGGACACCACCTGG
ACATCACGCCTCCGCTGCGT

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWIESPLRVAADGSVNLQSAPAPAPAEPQ
GNGEANADSARGSDDENYTDSGGDDYGSDWSLDDFARRPQGEDDEKTPMQLRDAEPTLREHLMEQLTPLKISLRDKGLAI
FLIESLDDDGYLSTSLDEICSELPEELEFEVDEVQAMLTLLQSFDPAGVGARNAAECLSLQLRRLQHPQRELALSIVQHH
LELLAVRDYTRLKKALQVDEAALKAAHELIRSLAPYPGHAYSRPEADFVVPDVFVRKSGSSWIAQLNPDVMPRLRINDMY
AQILRGAKGESGAAGLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKNFFTHGEIAMRPLVLREIADTLGLHESTISR
VTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEDPRSPLSDSRIAELLGEQGFVVARRTVAKYRE
ALKIPAVNLRKTL

Sequences:

>Translated_493_residues
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWIESPLRVAADGSVNLQSAPAPAPAEPQ
GNGEANADSARGSDDENYTDSGGDDYGSDWSLDDFARRPQGEDDEKTPMQLRDAEPTLREHLMEQLTPLKISLRDKGLAI
FLIESLDDDGYLSTSLDEICSELPEELEFEVDEVQAMLTLLQSFDPAGVGARNAAECLSLQLRRLQHPQRELALSIVQHH
LELLAVRDYTRLKKALQVDEAALKAAHELIRSLAPYPGHAYSRPEADFVVPDVFVRKSGSSWIAQLNPDVMPRLRINDMY
AQILRGAKGESGAAGLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKNFFTHGEIAMRPLVLREIADTLGLHESTISR
VTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEDPRSPLSDSRIAELLGEQGFVVARRTVAKYRE
ALKIPAVNLRKTL
>Mature_493_residues
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWIESPLRVAADGSVNLQSAPAPAPAEPQ
GNGEANADSARGSDDENYTDSGGDDYGSDWSLDDFARRPQGEDDEKTPMQLRDAEPTLREHLMEQLTPLKISLRDKGLAI
FLIESLDDDGYLSTSLDEICSELPEELEFEVDEVQAMLTLLQSFDPAGVGARNAAECLSLQLRRLQHPQRELALSIVQHH
LELLAVRDYTRLKKALQVDEAALKAAHELIRSLAPYPGHAYSRPEADFVVPDVFVRKSGSSWIAQLNPDVMPRLRINDMY
AQILRGAKGESGAAGLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKNFFTHGEIAMRPLVLREIADTLGLHESTISR
VTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEDPRSPLSDSRIAELLGEQGFVVARRTVAKYRE
ALKIPAVNLRKTL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=495, Percent_Identity=46.6666666666667, Blast_Score=401, Evalue=1e-113,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 54915; Mature: 54915

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWIESPLRV
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHE
AADGSVNLQSAPAPAPAEPQGNGEANADSARGSDDENYTDSGGDDYGSDWSLDDFARRPQ
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCC
GEDDEKTPMQLRDAEPTLREHLMEQLTPLKISLRDKGLAIFLIESLDDDGYLSTSLDEIC
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCEEEEEEECCCCCCCCHHHHHHHH
SELPEELEFEVDEVQAMLTLLQSFDPAGVGARNAAECLSLQLRRLQHPQRELALSIVQHH
HHCHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LELLAVRDYTRLKKALQVDEAALKAAHELIRSLAPYPGHAYSRPEADFVVPDVFVRKSGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCHHHHCCCCC
SWIAQLNPDVMPRLRINDMYAQILRGAKGESGAAGLQQKLQEARWLIKNIQQRFDTILRV
CCEECCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SQAIVERQKNFFTHGEIAMRPLVLREIADTLGLHESTISRVTTNKYMATPMGTFELKYFF
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEECCCCCEEEHHHH
GSHVSTETGGAASSTAIRALIKQLIGAEDPRSPLSDSRIAELLGEQGFVVARRTVAKYRE
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
ALKIPAVNLRKTL
HHCCCCCHHHHCC
>Mature Secondary Structure
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWIESPLRV
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHE
AADGSVNLQSAPAPAPAEPQGNGEANADSARGSDDENYTDSGGDDYGSDWSLDDFARRPQ
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCC
GEDDEKTPMQLRDAEPTLREHLMEQLTPLKISLRDKGLAIFLIESLDDDGYLSTSLDEIC
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCEEEEEEECCCCCCCCHHHHHHHH
SELPEELEFEVDEVQAMLTLLQSFDPAGVGARNAAECLSLQLRRLQHPQRELALSIVQHH
HHCHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LELLAVRDYTRLKKALQVDEAALKAAHELIRSLAPYPGHAYSRPEADFVVPDVFVRKSGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCHHHHCCCCC
SWIAQLNPDVMPRLRINDMYAQILRGAKGESGAAGLQQKLQEARWLIKNIQQRFDTILRV
CCEECCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SQAIVERQKNFFTHGEIAMRPLVLREIADTLGLHESTISRVTTNKYMATPMGTFELKYFF
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEECCCCCEEEHHHH
GSHVSTETGGAASSTAIRALIKQLIGAEDPRSPLSDSRIAELLGEQGFVVARRTVAKYRE
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
ALKIPAVNLRKTL
HHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1417413 [H]