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The map label for this gene is lolB

Identifier: 73540050

GI number: 73540050

Start: 372986

End: 373609

Strand: Reverse

Name: lolB

Synonym: Reut_A0344

Alternate gene names: 73540050

Gene position: 373609-372986 (Counterclockwise)

Preceding gene: 73540051

Following gene: 73540049

Centisome position: 9.81

GC content: 70.35

Gene sequence:

>624_bases
ATGCCGACCATGAACCGATCCCGCCGCCTGGCTCTGCTTTGCCTTGGCGCGCCCCTGCTGCTGGCGGCCTGCGCCAGCGT
TGCGCCCTCGCGCGGCTTCGATGCCGGTGAAGGCTCCGCCAGCCGGCACTACACGGGCCGCTTCTCGGCCAACTATGTCC
GCTACGGGCGCGACGAAGGCGTGCAAGGCAGCTTCCGTTGGGAAGAACAGGGCCGCAACGTACGGCTCGACCTGCTGTCG
CCGCTGGGCCAGACACTGGCCGTGGTCACGGCCACGCCATCGGGCGCGACGCTCGACCTGCCTAACAAGCCGCCGCGCAA
TGCGCCCGAAGTCGACAGCTTGCTTGAGGAAGCGCTCGGCTTCGCGCTGCCCGTGGCTGGCATGCGCGACTGGCTGCACG
GTCGGCCCGCACCAGGCCGACCGGCGAAATCCACGCGTGACACCGAGGGTCGACTTGCCACACTCGCCCAGGACGGCTGG
AGCGTGCGCTACGTGGCCTGGCAGGAAGTGCCATCGGCTTCGCAAGCGCCCGCCCCGCGGCCGCGCCGCATCGACCTGGA
ACGCGAAGGCGGCCCCACGCCGCTGGCCGTGAAGCTCGTGATCGATCCGGAGGAAGCGCCTTGA

Upstream 100 bases:

>100_bases
CGCTACAACGTCCTGCTGTCCAAGTAAGCCCGGGCCCGTTCCGGTGAACGGGCCCCGCATCCATAACGAGCCGCCCCGCT
GGGGCGGCGCGCTTTCCTGA

Downstream 100 bases:

>100_bases
CCGCAGCCTTGACCGCGGCACCCACCCTGCCGCCGCCGGAACTGCGCGACTGCCCCGCGCCAGCCAAGCTCAACCTGTTC
CTGCACGTCACGGGCCGGCG

Product: outer membrane lipoprotein LolB

Products: 5-aminolevulinate; CoA; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MPTMNRSRRLALLCLGAPLLLAACASVAPSRGFDAGEGSASRHYTGRFSANYVRYGRDEGVQGSFRWEEQGRNVRLDLLS
PLGQTLAVVTATPSGATLDLPNKPPRNAPEVDSLLEEALGFALPVAGMRDWLHGRPAPGRPAKSTRDTEGRLATLAQDGW
SVRYVAWQEVPSASQAPAPRPRRIDLEREGGPTPLAVKLVIDPEEAP

Sequences:

>Translated_207_residues
MPTMNRSRRLALLCLGAPLLLAACASVAPSRGFDAGEGSASRHYTGRFSANYVRYGRDEGVQGSFRWEEQGRNVRLDLLS
PLGQTLAVVTATPSGATLDLPNKPPRNAPEVDSLLEEALGFALPVAGMRDWLHGRPAPGRPAKSTRDTEGRLATLAQDGW
SVRYVAWQEVPSASQAPAPRPRRIDLEREGGPTPLAVKLVIDPEEAP
>Mature_206_residues
PTMNRSRRLALLCLGAPLLLAACASVAPSRGFDAGEGSASRHYTGRFSANYVRYGRDEGVQGSFRWEEQGRNVRLDLLSP
LGQTLAVVTATPSGATLDLPNKPPRNAPEVDSLLEEALGFALPVAGMRDWLHGRPAPGRPAKSTRDTEGRLATLAQDGWS
VRYVAWQEVPSASQAPAPRPRRIDLEREGGPTPLAVKLVIDPEEAP

Specific function: Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the lolA protein

COG id: COG3017

COG function: function code M; Outer membrane lipoprotein involved in outer membrane biogenesis

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lolB family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LOLB_CUPPJ (Q476F7)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_294570.1
- ProteinModelPortal:   Q476F7
- SMR:   Q476F7
- GeneID:   3609585
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A0344
- NMPDR:   fig|264198.3.peg.856
- HOGENOM:   HBG451855
- OMA:   PRNAPEV
- ProtClustDB:   PRK00022
- BioCyc:   REUT264198:REUT_A0344-MONOMER
- HAMAP:   MF_00233
- InterPro:   IPR004565
- TIGRFAMs:   TIGR00548

Pfam domain/function: PF03550 LolB

EC number: NA

Molecular weight: Translated: 22300; Mature: 22169

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTMNRSRRLALLCLGAPLLLAACASVAPSRGFDAGEGSASRHYTGRFSANYVRYGRDEG
CCCCCCCCEEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCEEECCCCC
VQGSFRWEEQGRNVRLDLLSPLGQTLAVVTATPSGATLDLPNKPPRNAPEVDSLLEEALG
CCCCEEHHCCCCEEEEEHHHCCCCEEEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHH
FALPVAGMRDWLHGRPAPGRPAKSTRDTEGRLATLAQDGWSVRYVAWQEVPSASQAPAPR
HHCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCC
PRRIDLEREGGPTPLAVKLVIDPEEAP
CCEEEEECCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure 
PTMNRSRRLALLCLGAPLLLAACASVAPSRGFDAGEGSASRHYTGRFSANYVRYGRDEG
CCCCCCCEEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCEEECCCCC
VQGSFRWEEQGRNVRLDLLSPLGQTLAVVTATPSGATLDLPNKPPRNAPEVDSLLEEALG
CCCCEEHHCCCCEEEEEHHHCCCCEEEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHH
FALPVAGMRDWLHGRPAPGRPAKSTRDTEGRLATLAQDGWSVRYVAWQEVPSASQAPAPR
HHCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCC
PRRIDLEREGGPTPLAVKLVIDPEEAP
CCEEEEECCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Succinyl-CoA; glycine [C]

Specific reaction: Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA