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The map label for this gene is nrtB [H]

Identifier: 73540035

GI number: 73540035

Start: 358500

End: 359402

Strand: Reverse

Name: nrtB [H]

Synonym: Reut_A0329

Alternate gene names: 73540035

Gene position: 359402-358500 (Counterclockwise)

Preceding gene: 73540036

Following gene: 73540034

Centisome position: 9.44

GC content: 66.89

Gene sequence:

>903_bases
GTGAATGCCATCGCCAGACCCGCGCCGGAAGGCGCTACCGGCCTGAAGGCCGACCCCGATGCCGGCGAACGCGCGCGGCG
ACGTGCGCTGGAAATCGAAGCGCAGGCCCGCCGTGCCGAACGGCGCGATGTACTGCGCGGGGTCGTGCTGAGAGGCGTAC
CGCCGCTGCTGGGCTTCGCGCTGTTCGTGCTGGCGTGGCACGGCATCGCCACGATGATCCCCGCCATTCCCACTCCGGGC
GCCACCTGGAACGCCGCTGTGCCGCTCTTCGCCGATCCGTTCTACCGCAATGGCCCCAATGACCAGGGCGTCGGCTGGAA
CGTGCTGGCATCGCTTGCTCGCGTCGGCGCCGGCTTCGGGCTCGCGGCGCTGATCGGCATCCCGGCGGGCTTCATCCTCG
GACGCTTCGCCTTCCTGAACGCGATGGCGTCGCCCGTGATCAGCCTGCTGCGGCCGGTTTCGCCGCTGGCATGGCTGCCG
ATCGGGCTGCTGCTATTCAAGGCTGCAAACCCGGCCGCGATCTGGGCCATCTTCATCTGCTCGATCTGGCCGATGGTGAT
CAACACCGCGGTCGGCGTCACCCGTGTGCCACAGGACTACCTGAACGTGGCGCGCGTGCTCGACCTGTCGGAATGGAAGG
TATTCACGCGCGTGCTGTTCCCCGCCGTGCTGCCCTACATGCTGACCGGCGTGCGGCTGTCGATCGGCACTGCATGGCTC
GTAATCGTGGCCGCGGAGATGCTGACCGGCGGCACCGGCATCGGCTTCTGGCTGTGGGACGAATGGAACAACCTGAAGGT
CGAGCACATCGTCATTGCGATCTTCGTAATCGGCATCATCGGCCTGCTGCTGGAACATGCGCTGCTGGCGCTGGCCCGGC
GCTTCAACTACGGCAACAACTGA

Upstream 100 bases:

>100_bases
ACCCGGGACCCGAAGGCCTACGCCGACGGCTTCAAGATCAAGGCCTGATGCGCAGGCTGCCGCCATTGTTTCGGATTGCC
CCTGATTTAAGGAGCCAGAC

Downstream 100 bases:

>100_bases
CTGCCCCGCGCGCCAACCCAACCGGACATCGCCATGGAAAAGTTCGTCAGCATCGAAAACGTCGGGCAGACCTTCAAGAC
ACGCAAGGGGCCGTTCGTGG

Product: nitrate transport permease

Products: taurine [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MNAIARPAPEGATGLKADPDAGERARRRALEIEAQARRAERRDVLRGVVLRGVPPLLGFALFVLAWHGIATMIPAIPTPG
ATWNAAVPLFADPFYRNGPNDQGVGWNVLASLARVGAGFGLAALIGIPAGFILGRFAFLNAMASPVISLLRPVSPLAWLP
IGLLLFKAANPAAIWAIFICSIWPMVINTAVGVTRVPQDYLNVARVLDLSEWKVFTRVLFPAVLPYMLTGVRLSIGTAWL
VIVAAEMLTGGTGIGFWLWDEWNNLKVEHIVIAIFVIGIIGLLLEHALLALARRFNYGNN

Sequences:

>Translated_300_residues
MNAIARPAPEGATGLKADPDAGERARRRALEIEAQARRAERRDVLRGVVLRGVPPLLGFALFVLAWHGIATMIPAIPTPG
ATWNAAVPLFADPFYRNGPNDQGVGWNVLASLARVGAGFGLAALIGIPAGFILGRFAFLNAMASPVISLLRPVSPLAWLP
IGLLLFKAANPAAIWAIFICSIWPMVINTAVGVTRVPQDYLNVARVLDLSEWKVFTRVLFPAVLPYMLTGVRLSIGTAWL
VIVAAEMLTGGTGIGFWLWDEWNNLKVEHIVIAIFVIGIIGLLLEHALLALARRFNYGNN
>Mature_300_residues
MNAIARPAPEGATGLKADPDAGERARRRALEIEAQARRAERRDVLRGVVLRGVPPLLGFALFVLAWHGIATMIPAIPTPG
ATWNAAVPLFADPFYRNGPNDQGVGWNVLASLARVGAGFGLAALIGIPAGFILGRFAFLNAMASPVISLLRPVSPLAWLP
IGLLLFKAANPAAIWAIFICSIWPMVINTAVGVTRVPQDYLNVARVLDLSEWKVFTRVLFPAVLPYMLTGVRLSIGTAWL
VIVAAEMLTGGTGIGFWLWDEWNNLKVEHIVIAIFVIGIIGLLLEHALLALARRFNYGNN

Specific function: Probably part of a high-affinity binding-protein- dependent transport system for nitrate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0600

COG function: function code P; ABC-type nitrate/sulfonate/bicarbonate transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786564, Length=200, Percent_Identity=33.5, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87081802, Length=147, Percent_Identity=29.9319727891156, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005889 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32408; Mature: 32408

Theoretical pI: Translated: 10.64; Mature: 10.64

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAIARPAPEGATGLKADPDAGERARRRALEIEAQARRAERRDVLRGVVLRGVPPLLGFA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LFVLAWHGIATMIPAIPTPGATWNAAVPLFADPFYRNGPNDQGVGWNVLASLARVGAGFG
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCHH
LAALIGIPAGFILGRFAFLNAMASPVISLLRPVSPLAWLPIGLLLFKAANPAAIWAIFIC
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
SIWPMVINTAVGVTRVPQDYLNVARVLDLSEWKVFTRVLFPAVLPYMLTGVRLSIGTAWL
HHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIVAAEMLTGGTGIGFWLWDEWNNLKVEHIVIAIFVIGIIGLLLEHALLALARRFNYGNN
HHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNAIARPAPEGATGLKADPDAGERARRRALEIEAQARRAERRDVLRGVVLRGVPPLLGFA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LFVLAWHGIATMIPAIPTPGATWNAAVPLFADPFYRNGPNDQGVGWNVLASLARVGAGFG
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCHH
LAALIGIPAGFILGRFAFLNAMASPVISLLRPVSPLAWLPIGLLLFKAANPAAIWAIFIC
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
SIWPMVINTAVGVTRVPQDYLNVARVLDLSEWKVFTRVLFPAVLPYMLTGVRLSIGTAWL
HHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIVAAEMLTGGTGIGFWLWDEWNNLKVEHIVIAIFVIGIIGLLLEHALLALARRFNYGNN
HHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: taurine [Periplasm]; ATP; H2O [C]

Specific reaction: taurine [Periplasm] + ATP + H2O = taurine [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]