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The map label for this gene is selU

Identifier: 73540007

GI number: 73540007

Start: 333231

End: 334331

Strand: Direct

Name: selU

Synonym: Reut_A0301

Alternate gene names: 73540007

Gene position: 333231-334331 (Clockwise)

Preceding gene: 73540006

Following gene: 73540011

Centisome position: 8.75

GC content: 66.12

Gene sequence:

>1101_bases
ATGCGTGAAGATACCGGCGATTTCCGCCGATTGTTCCTGAGCGGCACGCCGATGATGGACGTGCGCGCACCGCTCGAATT
CGCGCGTGGCGCGTTCCCGGGCACCGTGAACCTGCCGCTGATGGATGACGAAGAGCGGCATCAGGTCGGCCTTTGCTATG
CGGAAAAAGGGCAGCACGCGGCGATTGAACTCGGCCATCGGCTGGTGTGGGGCGCTCTCAAGGCGACACGTATCGCGCAA
TGGGCGGATTTTGCCCGCGCCCATCCGGACGGCTATCTGTATTGCTTCCGCGGCGGACTGCGCTCGCAGATCGTGCAGCA
GTGGCTCCGCGACGAAGCGGGTATCGACTATCCGCGCGTGACCGGCGGCTACAAGGCGATGCGTGGCTTCCTGATCGACG
TGATTGAGCAGGCCGCGGCCAAGCAGGACTTTCTTGTGCTGGGCGGCCTGACCGGCACCGGCAAGACGGATGTGATTGCC
GAAGTGCCGGCGGCTGTGGATCTTGAAGGGCTAGCCTGCCACCGTGGCTCGGCATTCGGTCGCCGCGCGCAACCACAGCC
GCAGCAGATCGACTTCGAAAATTCGCTGGCCATCGATGTGCTGCAGCGTGCCGAGGCCGGCTACCGTGCGCTGGTGGTCG
AGGACGAGGGCCGCTTCATCGGTGGCCGGGACTTGCCCAAAGTGCTGTGGCAGCGCATGCAGGCCAGCCCGATGGTGTGG
CTGGAGGCGTCGTTCGAGGAACGCGTGGAGCGTGTCTTGCGCGACTATGTGCTGGGGCTGGCTGCCGAGCATATCGAGCA
GCTTGGTCCGGTGGCCGGCTTCGAGGCGTATGCCACGCGTTTGCGTGACGCCATGGCCGCCATCTCCCCGCGGCTCGGCA
GTGAACGCTATGGCCGTTTGTCGGCGTTGCTGGAGCAGGCGCTGACGAGCCAGTCGGAGCGGGGGGAAACCGCGCCGCAC
CGCGCCTGGATCGAAGTGCTGCTGCGCGACTACTACGATCCGATGTACGCGTACCAGGAAGAGAAACGCGCCGCGCGTGT
GGTGTTCCGCGGCGACCGCGCGGCGGTCACCGACTGGCTGCGCGAGCGCTCGGCACGCTGA

Upstream 100 bases:

>100_bases
GAGAGGCGGCATTCCTCGATGCCTGCACCCGGCTTGGCCTGGACCTCGCGCCCATCGGCGAACTGGCCGCACGCGGCGAG
CATGCCGTAGAGGTCGCCTG

Downstream 100 bases:

>100_bases
TGCCTGTCAATGCCCGGCGCAAACCGGGCAGTCGGGTGTGCGCGCGAGCTGCATCGTGGTCCACTCCATCGTCATCGCAT
TGACCATCAGCAGCCGGCCG

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MREDTGDFRRLFLSGTPMMDVRAPLEFARGAFPGTVNLPLMDDEERHQVGLCYAEKGQHAAIELGHRLVWGALKATRIAQ
WADFARAHPDGYLYCFRGGLRSQIVQQWLRDEAGIDYPRVTGGYKAMRGFLIDVIEQAAAKQDFLVLGGLTGTGKTDVIA
EVPAAVDLEGLACHRGSAFGRRAQPQPQQIDFENSLAIDVLQRAEAGYRALVVEDEGRFIGGRDLPKVLWQRMQASPMVW
LEASFEERVERVLRDYVLGLAAEHIEQLGPVAGFEAYATRLRDAMAAISPRLGSERYGRLSALLEQALTSQSERGETAPH
RAWIEVLLRDYYDPMYAYQEEKRAARVVFRGDRAAVTDWLRERSAR

Sequences:

>Translated_366_residues
MREDTGDFRRLFLSGTPMMDVRAPLEFARGAFPGTVNLPLMDDEERHQVGLCYAEKGQHAAIELGHRLVWGALKATRIAQ
WADFARAHPDGYLYCFRGGLRSQIVQQWLRDEAGIDYPRVTGGYKAMRGFLIDVIEQAAAKQDFLVLGGLTGTGKTDVIA
EVPAAVDLEGLACHRGSAFGRRAQPQPQQIDFENSLAIDVLQRAEAGYRALVVEDEGRFIGGRDLPKVLWQRMQASPMVW
LEASFEERVERVLRDYVLGLAAEHIEQLGPVAGFEAYATRLRDAMAAISPRLGSERYGRLSALLEQALTSQSERGETAPH
RAWIEVLLRDYYDPMYAYQEEKRAARVVFRGDRAAVTDWLRERSAR
>Mature_366_residues
MREDTGDFRRLFLSGTPMMDVRAPLEFARGAFPGTVNLPLMDDEERHQVGLCYAEKGQHAAIELGHRLVWGALKATRIAQ
WADFARAHPDGYLYCFRGGLRSQIVQQWLRDEAGIDYPRVTGGYKAMRGFLIDVIEQAAAKQDFLVLGGLTGTGKTDVIA
EVPAAVDLEGLACHRGSAFGRRAQPQPQQIDFENSLAIDVLQRAEAGYRALVVEDEGRFIGGRDLPKVLWQRMQASPMVW
LEASFEERVERVLRDYVLGLAAEHIEQLGPVAGFEAYATRLRDAMAAISPRLGSERYGRLSALLEQALTSQSERGETAPH
RAWIEVLLRDYYDPMYAYQEEKRAARVVFRGDRAAVTDWLRERSAR

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain

Homologues:

Organism=Escherichia coli, GI1786712, Length=363, Percent_Identity=46.2809917355372, Blast_Score=315, Evalue=4e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SELU_CUPPJ (Q476K0)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_294527.1
- ProteinModelPortal:   Q476K0
- GeneID:   3609846
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A0301
- NMPDR:   fig|264198.3.peg.813
- HOGENOM:   HBG452364
- OMA:   NLPLMND
- ProtClustDB:   PRK11784
- BioCyc:   REUT264198:REUT_A0301-MONOMER
- HAMAP:   MF_01622
- InterPro:   IPR001763
- InterPro:   IPR017582
- Gene3D:   G3DSA:3.40.250.10
- SMART:   SM00450
- TIGRFAMs:   TIGR03167

Pfam domain/function: SSF52821 Rhodanese-like

EC number: NA

Molecular weight: Translated: 41128; Mature: 41128

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS50206 RHODANESE_3

Important sites: ACT_SITE 95-95

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREDTGDFRRLFLSGTPMMDVRAPLEFARGAFPGTVNLPLMDDEERHQVGLCYAEKGQHA
CCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCCHHEEEEECCCCCCH
AIELGHRLVWGALKATRIAQWADFARAHPDGYLYCFRGGLRSQIVQQWLRDEAGIDYPRV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCC
TGGYKAMRGFLIDVIEQAAAKQDFLVLGGLTGTGKTDVIAEVPAAVDLEGLACHRGSAFG
CCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHCCCCCCCCCHHCCCCCCCC
RRAQPQPQQIDFENSLAIDVLQRAEAGYRALVVEDEGRFIGGRDLPKVLWQRMQASPMVW
CCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCEECCCCHHHHHHHHHCCCCEEE
LEASFEERVERVLRDYVLGLAAEHIEQLGPVAGFEAYATRLRDAMAAISPRLGSERYGRL
EECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHH
SALLEQALTSQSERGETAPHRAWIEVLLRDYYDPMYAYQEEKRAARVVFRGDRAAVTDWL
HHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCHHHHHHHH
RERSAR
HHHCCC
>Mature Secondary Structure
MREDTGDFRRLFLSGTPMMDVRAPLEFARGAFPGTVNLPLMDDEERHQVGLCYAEKGQHA
CCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCCHHEEEEECCCCCCH
AIELGHRLVWGALKATRIAQWADFARAHPDGYLYCFRGGLRSQIVQQWLRDEAGIDYPRV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCC
TGGYKAMRGFLIDVIEQAAAKQDFLVLGGLTGTGKTDVIAEVPAAVDLEGLACHRGSAFG
CCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHCCCCCCCCCHHCCCCCCCC
RRAQPQPQQIDFENSLAIDVLQRAEAGYRALVVEDEGRFIGGRDLPKVLWQRMQASPMVW
CCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCEECCCCHHHHHHHHHCCCCEEE
LEASFEERVERVLRDYVLGLAAEHIEQLGPVAGFEAYATRLRDAMAAISPRLGSERYGRL
EECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHH
SALLEQALTSQSERGETAPHRAWIEVLLRDYYDPMYAYQEEKRAARVVFRGDRAAVTDWL
HHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCHHHHHHHH
RERSAR
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA