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The map label for this gene is gshB [H]

Identifier: 73540000

GI number: 73540000

Start: 327356

End: 328309

Strand: Direct

Name: gshB [H]

Synonym: Reut_A0294

Alternate gene names: 73540000

Gene position: 327356-328309 (Clockwise)

Preceding gene: 73539999

Following gene: 73540001

Centisome position: 8.6

GC content: 64.57

Gene sequence:

>954_bases
ATGCGCATCCTCTTCATCACCGATCCGCTGGAGACCTTCAAGACCTACAAGGACTCCACCTACGCCATGATGACCGAGGC
CGCGGCGCGCGGCCATCACGTGTTCTGGAGCCTGCAGTCCCAGTTGTCGCTGTTTGGCGACCAGGTCGAGACTGTAGCGA
CCCCGCTGCAGCTCACCGGCGACAGCCATGACTGGTACCGCGAAGGCACGCCGGCGCTGACGTCGCTGAAGGACTTCGAC
GCGGTGCTGATGCGCAAGGATCCGCCGTTCGACATGGAATACGTCACCAGCACCTGGCTGCTGGAACTGGCGGAGTCGCA
GGGTGCGCGCGTGTTCAACAAGCCGCGCGCGATCCGCGACCATTCCGAGAAGCTGGCCATCAACCAGTTTCCGGAATTCA
TCACGCCGACGCTGGTCACGCGCGACATCGCGCGCATCCGCGACTTCCACGCCGAACACCGCGACATCATCATCAAGCCG
CTCGACGGCATGGGTGGCATGGGCGTGTTCCGTGTCGGCGCGGACGGCATGAACCTCGGGGCCATCGTCGAAACGCTTGG
CGAAGATGGCCGTCGCACGCTGATGATCCAGCGCTATATCCCGGCGATCAAGGACGGTGACAAGCGCATCCTGCTGATCG
GCGGCGTGCCCGTGCCGTATTCGCTGGCGCGAGTTCCCATGGCGGGCGAAGTGCGCGGCAACCTGGCGGCGGGCGGCACC
GGCCGCGCGCAGGAACTGACCGTGCGCGACCGCGACATTGCGGAGGCGCTGGCGCCGGGCCTGTGGGAGCAAGGTTTGCT
GCTGGTGGGTCTCGATGTGATCGGCGATTACCTCACAGAAGTCAATGTGACGAGTCCGACCTGTTTCCAGGAAATCACCC
AGCAGACCGGTTTTCAAGTAGCCGCCATGTTTATCGACGCGCTCGAGCACGCCGTGGAGGCGCCGGGCGCCTGA

Upstream 100 bases:

>100_bases
CCGCTTCTATATGTACGGCGTAGTAGCTCGGCTGGCGCTGCTGGCGGCCTCGATCGAACTCGAAAAGACCGACCCGAACC
CGATTCTCTGACGAGCCCCC

Downstream 100 bases:

>100_bases
GCGTTACAAGCGGGCGAAGGCCGGCAGAAAGCGGGCGTAGGCGCGAAAATACGGGGGAGGGCGCCTGCTACAATCGGGCC
AGTCCAACGGATTCACCATC

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MRILFITDPLETFKTYKDSTYAMMTEAAARGHHVFWSLQSQLSLFGDQVETVATPLQLTGDSHDWYREGTPALTSLKDFD
AVLMRKDPPFDMEYVTSTWLLELAESQGARVFNKPRAIRDHSEKLAINQFPEFITPTLVTRDIARIRDFHAEHRDIIIKP
LDGMGGMGVFRVGADGMNLGAIVETLGEDGRRTLMIQRYIPAIKDGDKRILLIGGVPVPYSLARVPMAGEVRGNLAAGGT
GRAQELTVRDRDIAEALAPGLWEQGLLLVGLDVIGDYLTEVNVTSPTCFQEITQQTGFQVAAMFIDALEHAVEAPGA

Sequences:

>Translated_317_residues
MRILFITDPLETFKTYKDSTYAMMTEAAARGHHVFWSLQSQLSLFGDQVETVATPLQLTGDSHDWYREGTPALTSLKDFD
AVLMRKDPPFDMEYVTSTWLLELAESQGARVFNKPRAIRDHSEKLAINQFPEFITPTLVTRDIARIRDFHAEHRDIIIKP
LDGMGGMGVFRVGADGMNLGAIVETLGEDGRRTLMIQRYIPAIKDGDKRILLIGGVPVPYSLARVPMAGEVRGNLAAGGT
GRAQELTVRDRDIAEALAPGLWEQGLLLVGLDVIGDYLTEVNVTSPTCFQEITQQTGFQVAAMFIDALEHAVEAPGA
>Mature_317_residues
MRILFITDPLETFKTYKDSTYAMMTEAAARGHHVFWSLQSQLSLFGDQVETVATPLQLTGDSHDWYREGTPALTSLKDFD
AVLMRKDPPFDMEYVTSTWLLELAESQGARVFNKPRAIRDHSEKLAINQFPEFITPTLVTRDIARIRDFHAEHRDIIIKP
LDGMGGMGVFRVGADGMNLGAIVETLGEDGRRTLMIQRYIPAIKDGDKRILLIGGVPVPYSLARVPMAGEVRGNLAAGGT
GRAQELTVRDRDIAEALAPGLWEQGLLLVGLDVIGDYLTEVNVTSPTCFQEITQQTGFQVAAMFIDALEHAVEAPGA

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=309, Percent_Identity=45.6310679611651, Blast_Score=287, Evalue=6e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35006; Mature: 35006

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILFITDPLETFKTYKDSTYAMMTEAAARGHHVFWSLQSQLSLFGDQVETVATPLQLTG
CEEEEEECCHHHHHHHCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCEEECC
DSHDWYREGTPALTSLKDFDAVLMRKDPPFDMEYVTSTWLLELAESQGARVFNKPRAIRD
CCCCHHHCCCCHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCHHHC
HSEKLAINQFPEFITPTLVTRDIARIRDFHAEHRDIIIKPLDGMGGMGVFRVGADGMNLG
CHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEECCCCCCHH
AIVETLGEDGRRTLMIQRYIPAIKDGDKRILLIGGVPVPYSLARVPMAGEVRGNLAAGGT
HHHHHHCCCCCEEEEHHHHCCCCCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCEECCCC
GRAQELTVRDRDIAEALAPGLWEQGLLLVGLDVIGDYLTEVNVTSPTCFQEITQQTGFQV
CCCEEEEECHHHHHHHHCCCCHHCCHHEEEHHHHHHHHHHCCCCCCHHHHHHHHHCCHHH
AAMFIDALEHAVEAPGA
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRILFITDPLETFKTYKDSTYAMMTEAAARGHHVFWSLQSQLSLFGDQVETVATPLQLTG
CEEEEEECCHHHHHHHCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCEEECC
DSHDWYREGTPALTSLKDFDAVLMRKDPPFDMEYVTSTWLLELAESQGARVFNKPRAIRD
CCCCHHHCCCCHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCHHHC
HSEKLAINQFPEFITPTLVTRDIARIRDFHAEHRDIIIKPLDGMGGMGVFRVGADGMNLG
CHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEECCCCCCHH
AIVETLGEDGRRTLMIQRYIPAIKDGDKRILLIGGVPVPYSLARVPMAGEVRGNLAAGGT
HHHHHHCCCCCEEEEHHHHCCCCCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCEECCCC
GRAQELTVRDRDIAEALAPGLWEQGLLLVGLDVIGDYLTEVNVTSPTCFQEITQQTGFQV
CCCEEEEECHHHHHHHHCCCCHHCCHHEEEHHHHHHHHHHCCCCCCHHHHHHHHHCCHHH
AAMFIDALEHAVEAPGA
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]