Click here to switch to the map view.

The map label for this gene is 73539999

Identifier: 73539999

GI number: 73539999

Start: 326051

End: 327346

Strand: Direct

Name: 73539999

Synonym: Reut_A0293

Alternate gene names: NA

Gene position: 326051-327346 (Clockwise)

Preceding gene: 73539998

Following gene: 73540000

Centisome position: 8.57

GC content: 62.42

Gene sequence:

>1296_bases
ATGGTCCCGCATCTCATTACCGCGCTGAACGGTCCGCTGCTCGAACTGGAGAAGAAGATCCTGGACGCGACGCCGTCCAT
CGAGCGCTGGTTCAGGCTGGAATGGCAGGAACATACGCCTCCGTTCTACTGCTCGGTGGACCTGCGCAATGCCGGTTTCA
AGCTCGCGCCGGTCGACACCAACCTGTTTCCGGGGGGCTTCAACAACCTGGCGCCTGAAATGCTGCCGCTGGCGGTGCAG
GCGGCCATGGCCGCGATCGAAAAGATCTGCCCGGATGCCAAGAACCTGCTGCTGATCCCCGAGCGGCACACGCGCAACAT
GTTCTACCTGCAGAACGTCGCACGGCTGTCGCTAATCATGCGCCAGGCGGGCCTTAATGTGCGCCTTGGGTCGCTGTCGG
AGGAAATCACCGAGCCCACGCCGATCGAACTACCGGATGGCCAGACCCTGGTGGTCGAGCCGCTGGCCCGGCTGGGCACC
AAGGACCGCCGGCTCGGGTTGAAGGATTTCGACCCGTGCTCGATCCTGTTGAACAACGACCTGTCGGCCGGCATTCCGCC
GATCCTCGAGAACATCAACGAGCAGTACCTGCTGCCGCCGCTGCACGCCGGCTGGTCCACGCGCCGCAAGAGCAGTCACT
TCGCCGCGTATGACGAAGTGGCCAAGAAGTTCGCCAAGCTGATCGATATCGACCAGTGGATGGTGAACCCGTACTTCGCG
CGCTGTTCGGGCGTGAATTTCCACGAGCGCATCGGCGAGGAAGAACTCGCGGATTCGGTGGAAGCGGTGCTCAAGAAGAT
CGCCAAGAAGTATCGCGAGTACGGGATCAAGGAAACGCCGTATGTGGTGGTGAAGGCCGATGCCGGTACGTATGGCATGG
GCATCATGACCGTGCGCGATCCGTCCGAGGTCAAGGGCCTGAACCGGAAGGAACGCAACAAGATGAGCGTCGTCAAGGAA
GGCCTGGAGGTCAGCGACGTCATCATCCAGGAAGGCGTGCACACGTTCGAGAAGGTCAACGAGGCCGTGGCCGAGCCGGT
GGTCTACATGATCGACCGCTACGTGGTCGGCGGCTTCTACCGCGTGCACACCGGCCGCGGCAACGACGAGAACCTGAATG
CGCCGGGCATGCATTTCGTGCCGCTTGCGTTCGCGCCCAACGGCATCCCGGACAGCCACGCCAAGCCGGGTGCCGCAGTG
CCGAACCGCTTCTATATGTACGGCGTAGTAGCTCGGCTGGCGCTGCTGGCGGCCTCGATCGAACTCGAAAAGACCGACCC
GAACCCGATTCTCTGA

Upstream 100 bases:

>100_bases
GCCGGGCATATCCTTATAAAGGGCTTGCCAGCAGCCAATTGGCCGTTCCTCTACAATCGGGGCGAACGATTAAGACGACC
AATAGATCAACAGGATGGAT

Downstream 100 bases:

>100_bases
CGAGCCCCCATGCGCATCCTCTTCATCACCGATCCGCTGGAGACCTTCAAGACCTACAAGGACTCCACCTACGCCATGAT
GACCGAGGCCGCGGCGCGCG

Product: glutamate--cysteine ligase GshA

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Glutamate-Cysteine Ligase GshA; Glutamate-Cysteine Ligase-Related Protein; Glutathione Synthetase; GshA Protein

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MVPHLITALNGPLLELEKKILDATPSIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDTNLFPGGFNNLAPEMLPLAVQ
AAMAAIEKICPDAKNLLLIPERHTRNMFYLQNVARLSLIMRQAGLNVRLGSLSEEITEPTPIELPDGQTLVVEPLARLGT
KDRRLGLKDFDPCSILLNNDLSAGIPPILENINEQYLLPPLHAGWSTRRKSSHFAAYDEVAKKFAKLIDIDQWMVNPYFA
RCSGVNFHERIGEEELADSVEAVLKKIAKKYREYGIKETPYVVVKADAGTYGMGIMTVRDPSEVKGLNRKERNKMSVVKE
GLEVSDVIIQEGVHTFEKVNEAVAEPVVYMIDRYVVGGFYRVHTGRGNDENLNAPGMHFVPLAFAPNGIPDSHAKPGAAV
PNRFYMYGVVARLALLAASIELEKTDPNPIL

Sequences:

>Translated_431_residues
MVPHLITALNGPLLELEKKILDATPSIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDTNLFPGGFNNLAPEMLPLAVQ
AAMAAIEKICPDAKNLLLIPERHTRNMFYLQNVARLSLIMRQAGLNVRLGSLSEEITEPTPIELPDGQTLVVEPLARLGT
KDRRLGLKDFDPCSILLNNDLSAGIPPILENINEQYLLPPLHAGWSTRRKSSHFAAYDEVAKKFAKLIDIDQWMVNPYFA
RCSGVNFHERIGEEELADSVEAVLKKIAKKYREYGIKETPYVVVKADAGTYGMGIMTVRDPSEVKGLNRKERNKMSVVKE
GLEVSDVIIQEGVHTFEKVNEAVAEPVVYMIDRYVVGGFYRVHTGRGNDENLNAPGMHFVPLAFAPNGIPDSHAKPGAAV
PNRFYMYGVVARLALLAASIELEKTDPNPIL
>Mature_431_residues
MVPHLITALNGPLLELEKKILDATPSIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDTNLFPGGFNNLAPEMLPLAVQ
AAMAAIEKICPDAKNLLLIPERHTRNMFYLQNVARLSLIMRQAGLNVRLGSLSEEITEPTPIELPDGQTLVVEPLARLGT
KDRRLGLKDFDPCSILLNNDLSAGIPPILENINEQYLLPPLHAGWSTRRKSSHFAAYDEVAKKFAKLIDIDQWMVNPYFA
RCSGVNFHERIGEEELADSVEAVLKKIAKKYREYGIKETPYVVVKADAGTYGMGIMTVRDPSEVKGLNRKERNKMSVVKE
GLEVSDVIIQEGVHTFEKVNEAVAEPVVYMIDRYVVGGFYRVHTGRGNDENLNAPGMHFVPLAFAPNGIPDSHAKPGAAV
PNRFYMYGVVARLALLAASIELEKTDPNPIL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 48063; Mature: 48063

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPHLITALNGPLLELEKKILDATPSIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDT
CCCCHHHHHCCCHHHHHHHHHCCCCCHHHHEEECHHCCCCCEEEEEEECCCCEEEEECCC
NLFPGGFNNLAPEMLPLAVQAAMAAIEKICPDAKNLLLIPERHTRNMFYLQNVARLSLIM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
RQAGLNVRLGSLSEEITEPTPIELPDGQTLVVEPLARLGTKDRRLGLKDFDPCSILLNND
HHCCCCEEECCCHHHHCCCCCCCCCCCCEEHCCHHHHCCCCHHCCCCCCCCCEEEEECCC
LSAGIPPILENINEQYLLPPLHAGWSTRRKSSHFAAYDEVAKKFAKLIDIDQWMVNPYFA
CCCCCCHHHHCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHH
RCSGVNFHERIGEEELADSVEAVLKKIAKKYREYGIKETPYVVVKADAGTYGMGIMTVRD
HHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECC
PSEVKGLNRKERNKMSVVKEGLEVSDVIIQEGVHTFEKVNEAVAEPVVYMIDRYVVGGFY
CHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
RVHTGRGNDENLNAPGMHFVPLAFAPNGIPDSHAKPGAAVPNRFYMYGVVARLALLAASI
EEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ELEKTDPNPIL
EEECCCCCCCC
>Mature Secondary Structure
MVPHLITALNGPLLELEKKILDATPSIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDT
CCCCHHHHHCCCHHHHHHHHHCCCCCHHHHEEECHHCCCCCEEEEEEECCCCEEEEECCC
NLFPGGFNNLAPEMLPLAVQAAMAAIEKICPDAKNLLLIPERHTRNMFYLQNVARLSLIM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
RQAGLNVRLGSLSEEITEPTPIELPDGQTLVVEPLARLGTKDRRLGLKDFDPCSILLNND
HHCCCCEEECCCHHHHCCCCCCCCCCCCEEHCCHHHHCCCCHHCCCCCCCCCEEEEECCC
LSAGIPPILENINEQYLLPPLHAGWSTRRKSSHFAAYDEVAKKFAKLIDIDQWMVNPYFA
CCCCCCHHHHCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHH
RCSGVNFHERIGEEELADSVEAVLKKIAKKYREYGIKETPYVVVKADAGTYGMGIMTVRD
HHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECC
PSEVKGLNRKERNKMSVVKEGLEVSDVIIQEGVHTFEKVNEAVAEPVVYMIDRYVVGGFY
CHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
RVHTGRGNDENLNAPGMHFVPLAFAPNGIPDSHAKPGAAVPNRFYMYGVVARLALLAASI
EEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ELEKTDPNPIL
EEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA