Click here to switch to the map view.

The map label for this gene is amtB [H]

Identifier: 73539998

GI number: 73539998

Start: 324070

End: 325611

Strand: Direct

Name: amtB [H]

Synonym: Reut_A0292

Alternate gene names: 73539998

Gene position: 324070-325611 (Clockwise)

Preceding gene: 73539997

Following gene: 73539999

Centisome position: 8.51

GC content: 67.51

Gene sequence:

>1542_bases
ATGACAACCTGGTTCAAGCGAATCCTGACGGCGGGCGCGATGGCGCTGGCCATCGGCACGGCCGGCGTCGGCCTGTCCAC
CCATGCCGTCGCGCAGGACAAGCCGGCGGCCGAGGCCTCCGCGCCGGCCGCCGCCGCTGCGCCTGCAGCGGCACCCGCTG
CTGCGGCTGCCCCCGCGGCTGCAACCACTGCCGCCGCACCGGCCGAGGCCGCTGCCGCTCCGGCAGCACCCGTTCCAAAC
AAGGGCGACACTGCCTGGCTGCTCGTGAGCACGGCGTTCGTGATCCTGATGACGCTGCCGGGCCTGGCGCTGTTCTATGG
CGGTCTGGTCCGTTCCAAGAACATGCTGTCGGTGCTGATGCAGTGCCTGGTGATCTTCTCGGTGGTGTCGCTGCTGTGGG
CCGTCTACGGCTACAGCTTTGCGTTCACCGAGGGCAATGCCTTCTTCGGCGGAACCGACCGGCTATTCATGAAGGGCCTG
ACGGTTGACGCCGTGGCGGCTACCTTCAGCAAGGGCGTGGTGGTGCCGGAGCTGGGCTACTTTGCATTCCAGTGCGCCTT
CGCGGCCATCACCTGCGGGCTGATCATCGGCGCCTTTGCCGAGCGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGC
TGTGGTTCACGTTCTCGTACATCCCCATCGCCCATATGGTCTGGTTCTGGCCGGGTCCGGACGCCTTCACTGACGCTGCC
GCCGGTACTGCTGCGACGGCCAAGTCGGGCTGGCTGTTCCAGAAGGGCGCGCTGGACTTCGCCGGCGGCACCGTGGTCCA
CATCAACGCCGCTGTGGCTGGCCTGGTTGGCGCCTTCATGTTCGGCAAGCGCATCGGCTTCGGCCGCGAGGCGATCCGTC
CGCACAGCCTGACCTTCACCATGGTCGGCGCCTCGCTGCTGTGGTTCGGCTGGTTCGGCTTCAATGCCGGCTCGGCGCTG
GAAGCCAACGGCTCGGCCGCACTGGCCTTCGTCAACACGCTGCTGGCCACCTGCGCCGCGGTGCTGTCCTGGACCTTCGG
CGAGTGGATCAGCAAGGGCAAGCCGTCGATGCTGGGCGGTGCCTCGGGTGCCGTGGCGGGCCTGGTGGCCATCACGCCGG
CAGCTGGCTTCGTCGGCCCGATGGGTTCGATCGCGATCGGCCTGATCGCCGGCCTGCTGTGCCTGTGGGGCGTGACCGGC
CTGAAGCGCATGCTGGGCATGGACGACTCGCTCGATGTGTTCGGCGTGCACGGCGTGGGCGGCATCCTGGGTGCGCTGCT
GACCGGCGTGTTCGCTTCGCCGAGCCTGGGCGGCACCGGCATCTACGACTACGTGGCCAACAAGGTGGCCGACGACTATT
CGATCGCCGGCCAGGTCTGGATCCAGTTCCAGGGCGTGCTGACCACGATCGTCTGGTCCGGTGTGGTGGCCTTCGTGGCA
TACAAGCTGGTGGATATGCTGATCGGCCTGCGGGTGCCGGAAGAAGAAGAGCGCGAGGGCCTGGATATCACCTCGCACGG
CGAAACCGCCTATGAAGTCTGA

Upstream 100 bases:

>100_bases
GCAAGATCTTCGTGGCCCCGATCGAGCAGGTCATCCGCATCCGCACCGGCGAGACCGGCAGCGATGCCCTGTGACAGCAC
AACGACAAGAGGTTAAGCAA

Downstream 100 bases:

>100_bases
TCGTCAAGGTGTTGTGCTGGAGAGCTGATTGGGAATCGACTCTCAGGAGTTTTGCCCGGCCGCGAGCCGGGCTTTTTTCA
TACGGCGAGCGGTTCAAGGC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MTTWFKRILTAGAMALAIGTAGVGLSTHAVAQDKPAAEASAPAAAAAPAAAPAAAAAPAAATTAAAPAEAAAAPAAPVPN
KGDTAWLLVSTAFVILMTLPGLALFYGGLVRSKNMLSVLMQCLVIFSVVSLLWAVYGYSFAFTEGNAFFGGTDRLFMKGL
TVDAVAATFSKGVVVPELGYFAFQCAFAAITCGLIIGAFAERAKFSAVLVFVVLWFTFSYIPIAHMVWFWPGPDAFTDAA
AGTAATAKSGWLFQKGALDFAGGTVVHINAAVAGLVGAFMFGKRIGFGREAIRPHSLTFTMVGASLLWFGWFGFNAGSAL
EANGSAALAFVNTLLATCAAVLSWTFGEWISKGKPSMLGGASGAVAGLVAITPAAGFVGPMGSIAIGLIAGLLCLWGVTG
LKRMLGMDDSLDVFGVHGVGGILGALLTGVFASPSLGGTGIYDYVANKVADDYSIAGQVWIQFQGVLTTIVWSGVVAFVA
YKLVDMLIGLRVPEEEEREGLDITSHGETAYEV

Sequences:

>Translated_513_residues
MTTWFKRILTAGAMALAIGTAGVGLSTHAVAQDKPAAEASAPAAAAAPAAAPAAAAAPAAATTAAAPAEAAAAPAAPVPN
KGDTAWLLVSTAFVILMTLPGLALFYGGLVRSKNMLSVLMQCLVIFSVVSLLWAVYGYSFAFTEGNAFFGGTDRLFMKGL
TVDAVAATFSKGVVVPELGYFAFQCAFAAITCGLIIGAFAERAKFSAVLVFVVLWFTFSYIPIAHMVWFWPGPDAFTDAA
AGTAATAKSGWLFQKGALDFAGGTVVHINAAVAGLVGAFMFGKRIGFGREAIRPHSLTFTMVGASLLWFGWFGFNAGSAL
EANGSAALAFVNTLLATCAAVLSWTFGEWISKGKPSMLGGASGAVAGLVAITPAAGFVGPMGSIAIGLIAGLLCLWGVTG
LKRMLGMDDSLDVFGVHGVGGILGALLTGVFASPSLGGTGIYDYVANKVADDYSIAGQVWIQFQGVLTTIVWSGVVAFVA
YKLVDMLIGLRVPEEEEREGLDITSHGETAYEV
>Mature_512_residues
TTWFKRILTAGAMALAIGTAGVGLSTHAVAQDKPAAEASAPAAAAAPAAAPAAAAAPAAATTAAAPAEAAAAPAAPVPNK
GDTAWLLVSTAFVILMTLPGLALFYGGLVRSKNMLSVLMQCLVIFSVVSLLWAVYGYSFAFTEGNAFFGGTDRLFMKGLT
VDAVAATFSKGVVVPELGYFAFQCAFAAITCGLIIGAFAERAKFSAVLVFVVLWFTFSYIPIAHMVWFWPGPDAFTDAAA
GTAATAKSGWLFQKGALDFAGGTVVHINAAVAGLVGAFMFGKRIGFGREAIRPHSLTFTMVGASLLWFGWFGFNAGSALE
ANGSAALAFVNTLLATCAAVLSWTFGEWISKGKPSMLGGASGAVAGLVAITPAAGFVGPMGSIAIGLIAGLLCLWGVTGL
KRMLGMDDSLDVFGVHGVGGILGALLTGVFASPSLGGTGIYDYVANKVADDYSIAGQVWIQFQGVLTTIVWSGVVAFVAY
KLVDMLIGLRVPEEEEREGLDITSHGETAYEV

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=452, Percent_Identity=54.646017699115, Blast_Score=478, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17550182, Length=461, Percent_Identity=29.2841648590022, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17550180, Length=451, Percent_Identity=29.2682926829268, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI32564042, Length=463, Percent_Identity=25.0539956803456, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI133931100, Length=451, Percent_Identity=24.6119733924612, Blast_Score=90, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324187, Length=450, Percent_Identity=34.8888888888889, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6325396, Length=434, Percent_Identity=33.1797235023041, Blast_Score=206, Evalue=9e-54,
Organism=Saccharomyces cerevisiae, GI6321559, Length=445, Percent_Identity=31.0112359550562, Blast_Score=202, Evalue=1e-52,
Organism=Drosophila melanogaster, GI161078314, Length=445, Percent_Identity=26.2921348314607, Blast_Score=111, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 52641; Mature: 52509

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTWFKRILTAGAMALAIGTAGVGLSTHAVAQDKPAAEASAPAAAAAPAAAPAAAAAPAA
CCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCHHCCCCCCCHHHCCCCH
ATTAAAPAEAAAAPAAPVPNKGDTAWLLVSTAFVILMTLPGLALFYGGLVRSKNMLSVLM
HHHCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
QCLVIFSVVSLLWAVYGYSFAFTEGNAFFGGTDRLFMKGLTVDAVAATFSKGVVVPELGY
HHHHHHHHHHHHHHHHCCEEEEECCCEEECCCHHHHHCCCCHHHHHHHHHCCCCCCCHHH
FAFQCAFAAITCGLIIGAFAERAKFSAVLVFVVLWFTFSYIPIAHMVWFWPGPDAFTDAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHC
AGTAATAKSGWLFQKGALDFAGGTVVHINAAVAGLVGAFMFGKRIGFGREAIRPHSLTFT
CCCCCCCCCCCEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHH
MVGASLLWFGWFGFNAGSALEANGSAALAFVNTLLATCAAVLSWTFGEWISKGKPSMLGG
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ASGAVAGLVAITPAAGFVGPMGSIAIGLIAGLLCLWGVTGLKRMLGMDDSLDVFGVHGVG
CCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHH
GILGALLTGVFASPSLGGTGIYDYVANKVADDYSIAGQVWIQFQGVLTTIVWSGVVAFVA
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHCCEEEEEHHHHHHHHHHHHHHHHHH
YKLVDMLIGLRVPEEEEREGLDITSHGETAYEV
HHHHHHHHCCCCCCHHHHCCCCEECCCCCCCCC
>Mature Secondary Structure 
TTWFKRILTAGAMALAIGTAGVGLSTHAVAQDKPAAEASAPAAAAAPAAAPAAAAAPAA
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCHHCCCCCCCHHHCCCCH
ATTAAAPAEAAAAPAAPVPNKGDTAWLLVSTAFVILMTLPGLALFYGGLVRSKNMLSVLM
HHHCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
QCLVIFSVVSLLWAVYGYSFAFTEGNAFFGGTDRLFMKGLTVDAVAATFSKGVVVPELGY
HHHHHHHHHHHHHHHHCCEEEEECCCEEECCCHHHHHCCCCHHHHHHHHHCCCCCCCHHH
FAFQCAFAAITCGLIIGAFAERAKFSAVLVFVVLWFTFSYIPIAHMVWFWPGPDAFTDAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHC
AGTAATAKSGWLFQKGALDFAGGTVVHINAAVAGLVGAFMFGKRIGFGREAIRPHSLTFT
CCCCCCCCCCCEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHH
MVGASLLWFGWFGFNAGSALEANGSAALAFVNTLLATCAAVLSWTFGEWISKGKPSMLGG
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ASGAVAGLVAITPAAGFVGPMGSIAIGLIAGLLCLWGVTGLKRMLGMDDSLDVFGVHGVG
CCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHH
GILGALLTGVFASPSLGGTGIYDYVANKVADDYSIAGQVWIQFQGVLTTIVWSGVVAFVA
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHCCEEEEEHHHHHHHHHHHHHHHHHH
YKLVDMLIGLRVPEEEEREGLDITSHGETAYEV
HHHHHHHHCCCCCCHHHHCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]