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The map label for this gene is metF [H]

Identifier: 73539921

GI number: 73539921

Start: 241569

End: 242399

Strand: Direct

Name: metF [H]

Synonym: Reut_A0215

Alternate gene names: 73539921

Gene position: 241569-242399 (Clockwise)

Preceding gene: 73539920

Following gene: 73539922

Centisome position: 6.35

GC content: 65.58

Gene sequence:

>831_bases
ATGCAAGACCGCTACTACAGTTTTGAATTCTTCCCGCCGAAGACGGCGGAGGGCGCCGAGAAGCTGCGCAACACGCGTGC
GCAGCTCGCCCCGCTCAACCCGAAGTACATCTCGGTGACGTTCGGCGCCGGCGGCACCACGCAGCAGGGCACGCTCGATA
CCGTGCTGGAAATCCAGCGCGAAGGCATCGAAGCCGCGCCGCACCTGTCGTGCGTGGGCTCGTCGCGCGAGAGCATCCGC
GAAATCCTGCAGCAGTACCGCGACGGTGGCATCCGCCATATCGTGGCGCTGCGCGGCGACATGCCGTCGGGCATGGGCGA
GATCGGCGAATTCCGCTACGCCAATGAACTGGTGGAGTTCATCCGCGCCGAGACCGGCGACTGGTTCCAGATCGAAGTGG
CGGCCTATCCGGAATACCATCCGCAGGCAAAATCGCCGCGCCACGACCTGGAGAACTTCGTGCGCAAGGTGAAGGCCGGC
GCGAACTCCGCCATCACGCAGTACTTCTTCAACGCCGACGCGTACTTCCGCTTCGTCGACGATGCGCGCGCGATGGGCGT
GGACGTGCCGATCGTCCCGGGCATCATGCCGATCACCAACTATTCGCAGCTCATGCGCTTCTCCGACATGTGCGGCTCGG
AGGTGCCGCGCTGGGTGGCCAAGCGGCTGGAGAGCTTTGGCGATGACCGCGAGTCGATCCGCGCGTTTGGGCTGGACGTG
GTCACGGCACTGTGCGAACGGCTGCTGGCCGGCGGCGTGCCGGGCCTGCATTTCTACACGCTCAACGCGGCCGGCGCCAC
CAAGGCGATCTGGCAGCGCCTGAAGCTCTGA

Upstream 100 bases:

>100_bases
GCTGCTGTCCAGCCTGCTGCTGGGCGAACGCGCGGCCTGACGGCCGCGCACTACCGCGGGTCCTACCAAGCGCGCTGTGC
CAACGCAGCGCGCCACCATC

Downstream 100 bases:

>100_bases
GCGTGGCCGCGCGCCGACCTGATCCCTTCATCGAACACCTGCTCGAACTGATGGGGCCGCTGGCGGCGCGCATCGGCGCT
ATTTCCGCGCGACGCATGTT

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MQDRYYSFEFFPPKTAEGAEKLRNTRAQLAPLNPKYISVTFGAGGTTQQGTLDTVLEIQREGIEAAPHLSCVGSSRESIR
EILQQYRDGGIRHIVALRGDMPSGMGEIGEFRYANELVEFIRAETGDWFQIEVAAYPEYHPQAKSPRHDLENFVRKVKAG
ANSAITQYFFNADAYFRFVDDARAMGVDVPIVPGIMPITNYSQLMRFSDMCGSEVPRWVAKRLESFGDDRESIRAFGLDV
VTALCERLLAGGVPGLHFYTLNAAGATKAIWQRLKL

Sequences:

>Translated_276_residues
MQDRYYSFEFFPPKTAEGAEKLRNTRAQLAPLNPKYISVTFGAGGTTQQGTLDTVLEIQREGIEAAPHLSCVGSSRESIR
EILQQYRDGGIRHIVALRGDMPSGMGEIGEFRYANELVEFIRAETGDWFQIEVAAYPEYHPQAKSPRHDLENFVRKVKAG
ANSAITQYFFNADAYFRFVDDARAMGVDVPIVPGIMPITNYSQLMRFSDMCGSEVPRWVAKRLESFGDDRESIRAFGLDV
VTALCERLLAGGVPGLHFYTLNAAGATKAIWQRLKL
>Mature_276_residues
MQDRYYSFEFFPPKTAEGAEKLRNTRAQLAPLNPKYISVTFGAGGTTQQGTLDTVLEIQREGIEAAPHLSCVGSSRESIR
EILQQYRDGGIRHIVALRGDMPSGMGEIGEFRYANELVEFIRAETGDWFQIEVAAYPEYHPQAKSPRHDLENFVRKVKAG
ANSAITQYFFNADAYFRFVDDARAMGVDVPIVPGIMPITNYSQLMRFSDMCGSEVPRWVAKRLESFGDDRESIRAFGLDV
VTALCERLLAGGVPGLHFYTLNAAGATKAIWQRLKL

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=283, Percent_Identity=41.3427561837456, Blast_Score=204, Evalue=8e-53,
Organism=Escherichia coli, GI1790377, Length=269, Percent_Identity=44.6096654275093, Blast_Score=238, Evalue=4e-64,
Organism=Caenorhabditis elegans, GI25147436, Length=286, Percent_Identity=32.5174825174825, Blast_Score=171, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI25147441, Length=286, Percent_Identity=32.5174825174825, Blast_Score=171, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6321313, Length=287, Percent_Identity=36.5853658536585, Blast_Score=174, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6325234, Length=284, Percent_Identity=34.1549295774648, Blast_Score=161, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24662658, Length=227, Percent_Identity=27.7533039647577, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 30885; Mature: 30885

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDRYYSFEFFPPKTAEGAEKLRNTRAQLAPLNPKYISVTFGAGGTTQQGTLDTVLEIQR
CCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHH
EGIEAAPHLSCVGSSRESIREILQQYRDGGIRHIVALRGDMPSGMGEIGEFRYANELVEF
HCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
IRAETGDWFQIEVAAYPEYHPQAKSPRHDLENFVRKVKAGANSAITQYFFNADAYFRFVD
HHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHEEEHH
DARAMGVDVPIVPGIMPITNYSQLMRFSDMCGSEVPRWVAKRLESFGDDRESIRAFGLDV
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
VTALCERLLAGGVPGLHFYTLNAAGATKAIWQRLKL
HHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure
MQDRYYSFEFFPPKTAEGAEKLRNTRAQLAPLNPKYISVTFGAGGTTQQGTLDTVLEIQR
CCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHH
EGIEAAPHLSCVGSSRESIREILQQYRDGGIRHIVALRGDMPSGMGEIGEFRYANELVEF
HCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
IRAETGDWFQIEVAAYPEYHPQAKSPRHDLENFVRKVKAGANSAITQYFFNADAYFRFVD
HHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHEEEHH
DARAMGVDVPIVPGIMPITNYSQLMRFSDMCGSEVPRWVAKRLESFGDDRESIRAFGLDV
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
VTALCERLLAGGVPGLHFYTLNAAGATKAIWQRLKL
HHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]