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The map label for this gene is thiD [H]

Identifier: 73539918

GI number: 73539918

Start: 238563

End: 239435

Strand: Reverse

Name: thiD [H]

Synonym: Reut_A0212

Alternate gene names: 73539918

Gene position: 239435-238563 (Counterclockwise)

Preceding gene: 73539924

Following gene: 73539917

Centisome position: 6.29

GC content: 68.5

Gene sequence:

>873_bases
ATGGCCACCGGCATTTTTTTCCGCTATTTCCTTGACATGACCCCGACACCACCCCGCACCCTCACCATCGCAGGTTCCGA
CTCGGGCGGCGGCGCCGGCATCCAGGCCGACCTCAAGACGTTTTCGGCACTCGGCTGCTTCGGCATGAGCGCCATCACCG
CGATCACGGCCCAGAACACGCTGGGCGTGACGGGCGTGCATGCGATTCCGGCCGATATGGTCACCGCGCAGATCGATGCG
GTCGCCTCGGATATCGGCGTCGATGCCGCCAAGACAGGCATGCTCGGCACCGCAGCCATTGTCGAGGCCGTGGCCGCGGC
AGCGGACCGCCACGGCATCCGCAAGCTCGTGGTTGACCCCGTGATGATCTCCACCTCGGGCGCGACGCTCTCGGATAACG
CTACCAGCCAGGCCATGGTGCGCCTGCTGTTCCCGCGTGCCGTGCTGGTGACGCCCAACCTCCCCGAGGCCTCCTACCTG
CTGGGGCGGCCGATCACGCGCCGTGCCGAGATGGAACAGGCCGCCGCCGACCTGATCGCGCTGGGCTGCCCCGCAGTGCT
GCTAAAGGGCGGCCACCTCGATGACGATGGCCCCGCGAGCGTTGCCAGCGGACTGGACGATCTGCTGATGCTGGCCGACG
GCACCGTGCGCGTCTTTACGCACCCGCGCATCGACACGAAGAACCTGCACGGTACCGGCTGCACGCTGGCTGCGGCCATC
GCCGCACAACTGGCACGCGGCGACGCGCTGGAAGAGGCCGTCAGCAAGGGACTGGACTTTGTGGCGCAGGCGATCGCCGC
AGGCGCCGGCCTCGAACTCGGTGCGGGCAACGGCCCGCTCAACCACGCCTTCGCACCGCGCGTGCTGGGCTGA

Upstream 100 bases:

>100_bases
GAAGACAGTCCGAGCCTGGCGGAGGGCATGTTACGCCACGATCCGTTGCAACGCTCCTCGGAGTGGCGGTATTGTAGCCT
GCCCGGCGTGGACCCGCACA

Downstream 100 bases:

>100_bases
ACGGAGACGACATGCAGCTATCCGACATCGACCTGCGCGAAGACCCTGCCGCCGCGCGCTACGTCAAGGAAGAGCAGGTA
CAGGTGATGTTTGCCGCCAA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MATGIFFRYFLDMTPTPPRTLTIAGSDSGGGAGIQADLKTFSALGCFGMSAITAITAQNTLGVTGVHAIPADMVTAQIDA
VASDIGVDAAKTGMLGTAAIVEAVAAAADRHGIRKLVVDPVMISTSGATLSDNATSQAMVRLLFPRAVLVTPNLPEASYL
LGRPITRRAEMEQAAADLIALGCPAVLLKGGHLDDDGPASVASGLDDLLMLADGTVRVFTHPRIDTKNLHGTGCTLAAAI
AAQLARGDALEEAVSKGLDFVAQAIAAGAGLELGAGNGPLNHAFAPRVLG

Sequences:

>Translated_290_residues
MATGIFFRYFLDMTPTPPRTLTIAGSDSGGGAGIQADLKTFSALGCFGMSAITAITAQNTLGVTGVHAIPADMVTAQIDA
VASDIGVDAAKTGMLGTAAIVEAVAAAADRHGIRKLVVDPVMISTSGATLSDNATSQAMVRLLFPRAVLVTPNLPEASYL
LGRPITRRAEMEQAAADLIALGCPAVLLKGGHLDDDGPASVASGLDDLLMLADGTVRVFTHPRIDTKNLHGTGCTLAAAI
AAQLARGDALEEAVSKGLDFVAQAIAAGAGLELGAGNGPLNHAFAPRVLG
>Mature_289_residues
ATGIFFRYFLDMTPTPPRTLTIAGSDSGGGAGIQADLKTFSALGCFGMSAITAITAQNTLGVTGVHAIPADMVTAQIDAV
ASDIGVDAAKTGMLGTAAIVEAVAAAADRHGIRKLVVDPVMISTSGATLSDNATSQAMVRLLFPRAVLVTPNLPEASYLL
GRPITRRAEMEQAAADLIALGCPAVLLKGGHLDDDGPASVASGLDDLLMLADGTVRVFTHPRIDTKNLHGTGCTLAAAIA
AQLARGDALEEAVSKGLDFVAQAIAAGAGLELGAGNGPLNHAFAPRVLG

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=267, Percent_Identity=49.438202247191, Blast_Score=223, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6325378, Length=279, Percent_Identity=36.9175627240143, Blast_Score=166, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6324997, Length=279, Percent_Identity=39.0681003584229, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6324517, Length=280, Percent_Identity=37.1428571428571, Blast_Score=154, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 29322; Mature: 29191

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATGIFFRYFLDMTPTPPRTLTIAGSDSGGGAGIQADLKTFSALGCFGMSAITAITAQNT
CCCHHEEHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
LGVTGVHAIPADMVTAQIDAVASDIGVDAAKTGMLGTAAIVEAVAAAADRHGIRKLVVDP
CCCCCCHHCCHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
VMISTSGATLSDNATSQAMVRLLFPRAVLVTPNLPEASYLLGRPITRRAEMEQAAADLIA
EEEECCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHHH
LGCPAVLLKGGHLDDDGPASVASGLDDLLMLADGTVRVFTHPRIDTKNLHGTGCTLAAAI
HCCHHHEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHH
AAQLARGDALEEAVSKGLDFVAQAIAAGAGLELGAGNGPLNHAFAPRVLG
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ATGIFFRYFLDMTPTPPRTLTIAGSDSGGGAGIQADLKTFSALGCFGMSAITAITAQNT
CCHHEEHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
LGVTGVHAIPADMVTAQIDAVASDIGVDAAKTGMLGTAAIVEAVAAAADRHGIRKLVVDP
CCCCCCHHCCHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
VMISTSGATLSDNATSQAMVRLLFPRAVLVTPNLPEASYLLGRPITRRAEMEQAAADLIA
EEEECCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHHH
LGCPAVLLKGGHLDDDGPASVASGLDDLLMLADGTVRVFTHPRIDTKNLHGTGCTLAAAI
HCCHHHEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHH
AAQLARGDALEEAVSKGLDFVAQAIAAGAGLELGAGNGPLNHAFAPRVLG
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]