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The map label for this gene is yieH [H]

Identifier: 73539848

GI number: 73539848

Start: 168526

End: 169233

Strand: Direct

Name: yieH [H]

Synonym: Reut_A0142

Alternate gene names: 73539848

Gene position: 168526-169233 (Clockwise)

Preceding gene: 73539847

Following gene: 73539849

Centisome position: 4.43

GC content: 66.24

Gene sequence:

>708_bases
ATGACTGCAGCAAACCGGGCCGATTCCGGCCTGACCTCCGGCCCATCCTCGCGTTTCGACTGTGTGATTTTCGACTGCGA
CGGCGTGCTCGTCGACAGCGAACCCATCGTCAACCGCGTGCTCAACGAAATGCTCAACGAGCTGGGCATCGCCATCTCGC
TCGAGGATTCGACCAAGATGTTCCTGGGGCGCGCGGTGCGCGAGGAACTCGGCAACATCGAACGCATGCGCGGCGCGCCG
CTGCCGGAGAACTGGCTGTCGCACTGGCTCGTGCGGCGCAACCAGGTGCTCGAGGCCGAGGTGCAGAGCGTGCCCTTCGT
ACGTGAAGCGGTGTCTGCGATTGCCGCGACCGGCATGCCGGTGTGCGTGGCATCGGGCGCGGATCGTATCAAGGTCAAGC
TGCAGCTCAAGCAAACAGGCCTGGTCGAACTGTTCCAGCAGGACGAGCGCGAGCATATCTTCTCGGCCACCGAGGTGGAG
CGCAGCAAGCCGGCGCCGGACGTGTACCTGCTCGCGGCGCGTACCATGGGCGTGGAGCCGTCGCGCTGCGCGGTGATCGA
AGACAGCCCGGCCGGCATCACCGCCGGCGTGGCCGCGGGCATGACCGTGTTTGGCTACGCGGCGCGCAACGATGCCGCCA
TGCTGCGTGCTGCCGGCGCGCATCACCTGTTCACGGATATGCGCGAACTGGCGGAGCTGCTCGGATGA

Upstream 100 bases:

>100_bases
TCGACCGGTTCAACCAGATTGAAGACCACCTGAAACGCGGCCGCCAGACCGAACTGCGCGCCGTGGCCAGCGTGGACAGC
AACAAGGAAAAGAAACCCGC

Downstream 100 bases:

>100_bases
GTGCCAGGCGCCCGCCCCGCGCCACGCCCACGCCGTGCCCGTGCGGCCTGGGCGCGGAATATGACGCGTGCTGCGGCCGC
TATCACCGCGGCGACGCCGT

Product: HAD family hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MTAANRADSGLTSGPSSRFDCVIFDCDGVLVDSEPIVNRVLNEMLNELGIAISLEDSTKMFLGRAVREELGNIERMRGAP
LPENWLSHWLVRRNQVLEAEVQSVPFVREAVSAIAATGMPVCVASGADRIKVKLQLKQTGLVELFQQDEREHIFSATEVE
RSKPAPDVYLLAARTMGVEPSRCAVIEDSPAGITAGVAAGMTVFGYAARNDAAMLRAAGAHHLFTDMRELAELLG

Sequences:

>Translated_235_residues
MTAANRADSGLTSGPSSRFDCVIFDCDGVLVDSEPIVNRVLNEMLNELGIAISLEDSTKMFLGRAVREELGNIERMRGAP
LPENWLSHWLVRRNQVLEAEVQSVPFVREAVSAIAATGMPVCVASGADRIKVKLQLKQTGLVELFQQDEREHIFSATEVE
RSKPAPDVYLLAARTMGVEPSRCAVIEDSPAGITAGVAAGMTVFGYAARNDAAMLRAAGAHHLFTDMRELAELLG
>Mature_234_residues
TAANRADSGLTSGPSSRFDCVIFDCDGVLVDSEPIVNRVLNEMLNELGIAISLEDSTKMFLGRAVREELGNIERMRGAPL
PENWLSHWLVRRNQVLEAEVQSVPFVREAVSAIAATGMPVCVASGADRIKVKLQLKQTGLVELFQQDEREHIFSATEVER
SKPAPDVYLLAARTMGVEPSRCAVIEDSPAGITAGVAAGMTVFGYAARNDAAMLRAAGAHHLFTDMRELAELLG

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates phosphoenolpyruvate and AMP [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1790151, Length=195, Percent_Identity=32.3076923076923, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789046, Length=190, Percent_Identity=30, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788021, Length=194, Percent_Identity=30.9278350515464, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082080, Length=203, Percent_Identity=29.5566502463054, Blast_Score=60, Evalue=9e-11,
Organism=Drosophila melanogaster, GI20129151, Length=233, Percent_Identity=27.0386266094421, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17137324, Length=194, Percent_Identity=29.8969072164948, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI116008157, Length=194, Percent_Identity=32.4742268041237, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 25439; Mature: 25308

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAANRADSGLTSGPSSRFDCVIFDCDGVLVDSEPIVNRVLNEMLNELGIAISLEDSTKM
CCCCCCCCCCCCCCCCCCCCEEEEECCCEEECCCHHHHHHHHHHHHHHCEEEEECCHHHH
FLGRAVREELGNIERMRGAPLPENWLSHWLVRRNQVLEAEVQSVPFVREAVSAIAATGMP
HHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
VCVASGADRIKVKLQLKQTGLVELFQQDEREHIFSATEVERSKPAPDVYLLAARTMGVEP
EEEECCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHCCCCC
SRCAVIEDSPAGITAGVAAGMTVFGYAARNDAAMLRAAGAHHLFTDMRELAELLG
CCEEEEECCCCCCHHHHHHHHHHHEECCCCCHHHHHHCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
TAANRADSGLTSGPSSRFDCVIFDCDGVLVDSEPIVNRVLNEMLNELGIAISLEDSTKM
CCCCCCCCCCCCCCCCCCCEEEEECCCEEECCCHHHHHHHHHHHHHHCEEEEECCHHHH
FLGRAVREELGNIERMRGAPLPENWLSHWLVRRNQVLEAEVQSVPFVREAVSAIAATGMP
HHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
VCVASGADRIKVKLQLKQTGLVELFQQDEREHIFSATEVERSKPAPDVYLLAARTMGVEP
EEEECCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHCCCCC
SRCAVIEDSPAGITAGVAAGMTVFGYAARNDAAMLRAAGAHHLFTDMRELAELLG
CCEEEEECCCCCCHHHHHHHHHHHEECCCCCHHHHHHCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]