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The map label for this gene is yiaJ [H]

Identifier: 73539819

GI number: 73539819

Start: 140228

End: 141061

Strand: Direct

Name: yiaJ [H]

Synonym: Reut_A0113

Alternate gene names: 73539819

Gene position: 140228-141061 (Clockwise)

Preceding gene: 73539810

Following gene: 73539820

Centisome position: 3.68

GC content: 67.99

Gene sequence:

>834_bases
ATGACCGAACTTGAGGAAGACGACGCCAAGCTGCGCTCCGGTATCCAGTCCATCGAGGTAGGGTTCAAGCTGCTGCAGGC
GTTGGCCGCCTCGCCGCGCGCGATGATGCTGCGCGACCTCGCCGCCGCGGCGGAGATGAATCCGGCCAAGGCCCATCGCT
ACCTGGTCAGCTTCCTGCGCCTCGGCGCGGTGGCGCAAGACCCGGTGAGCGGGCGCTATGATCTCGGGCCGTTCGCGCTG
CAGCTCGGGCTGGCCGGCCTGAACCGGCTGGATCCGGTCAAGAAGGCGCGGCCGATCCTGTCGCAGTTGCGTGACGAGAT
CGACCTGACCGCGGGCATCGCGGTGTGGGGCAACCACGGGCCCACCATCGTGCACTGGGAGGAATCGAGCCACCCGGTCA
CGGTCAGCCTGCGGCTCGGCGACGTGATGCCGATGCTCAACTCCGCCACCGGCCGCCTCTACGGCGCGTACCTGTCGCGC
AAGCAGACGCTGCCGCTGATCGAGCGCGAGCTGGCCGCGCGCGGCCAGGACGACGTGCCCGACATCCCGGCCAGCCTGTC
CGAATACGATGCGATCTGCGCCGATGTGCGCACACACGGCGCGGCGCGTACGCGCGGCGGCGTGCTGCCGGGCATCAACG
CGTTCTCGATGCCGGTGTTCGACGCCAACGGCCATCTGGCGATGGGTTTGATCGTGCTCGGCGCGCAAAGCATATTCGAT
GCAGAATGGAACGGTACGGTCGATCGCCGCGTGCGAGAGATCGCCAAACAGCTTTCATCGGAACTCGGCTACCTTGGCGC
CGCCCAAACGGCCGGACGGAACGGCCCCCTCTGA

Upstream 100 bases:

>100_bases
AAGGTTTTTGCCGGATCCCCTGTGCTGCCGGCGCTGAATACCCCACAATTCTGCCGTATCCCCCAATTTTTTCCATTCAC
GCCCCCAATGCTCGCCGCGC

Downstream 100 bases:

>100_bases
TTCTCGCGCCAGAACCGGCGCCGATCCGCGCGTTTGGCGGCGGCGGCGCTGGATCCTCGTCATCTTGCTGGTGCTGCTCG
TGCACATGCTGGCCGTGATC

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: YiaKLMNOPQRS operon repressor; YiaK-S operon repressor [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MTELEEDDAKLRSGIQSIEVGFKLLQALAASPRAMMLRDLAAAAEMNPAKAHRYLVSFLRLGAVAQDPVSGRYDLGPFAL
QLGLAGLNRLDPVKKARPILSQLRDEIDLTAGIAVWGNHGPTIVHWEESSHPVTVSLRLGDVMPMLNSATGRLYGAYLSR
KQTLPLIERELAARGQDDVPDIPASLSEYDAICADVRTHGAARTRGGVLPGINAFSMPVFDANGHLAMGLIVLGAQSIFD
AEWNGTVDRRVREIAKQLSSELGYLGAAQTAGRNGPL

Sequences:

>Translated_277_residues
MTELEEDDAKLRSGIQSIEVGFKLLQALAASPRAMMLRDLAAAAEMNPAKAHRYLVSFLRLGAVAQDPVSGRYDLGPFAL
QLGLAGLNRLDPVKKARPILSQLRDEIDLTAGIAVWGNHGPTIVHWEESSHPVTVSLRLGDVMPMLNSATGRLYGAYLSR
KQTLPLIERELAARGQDDVPDIPASLSEYDAICADVRTHGAARTRGGVLPGINAFSMPVFDANGHLAMGLIVLGAQSIFD
AEWNGTVDRRVREIAKQLSSELGYLGAAQTAGRNGPL
>Mature_276_residues
TELEEDDAKLRSGIQSIEVGFKLLQALAASPRAMMLRDLAAAAEMNPAKAHRYLVSFLRLGAVAQDPVSGRYDLGPFALQ
LGLAGLNRLDPVKKARPILSQLRDEIDLTAGIAVWGNHGPTIVHWEESSHPVTVSLRLGDVMPMLNSATGRLYGAYLSRK
QTLPLIERELAARGQDDVPDIPASLSEYDAICADVRTHGAARTRGGVLPGINAFSMPVFDANGHLAMGLIVLGAQSIFDA
EWNGTVDRRVREIAKQLSSELGYLGAAQTAGRNGPL

Specific function: Negatively controls the transcription of the yiaKLMNOPQRS operon, which may be involved in the utilization of 2,3-diketo-L-gulonate [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1789999, Length=256, Percent_Identity=25, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI87082359, Length=158, Percent_Identity=29.1139240506329, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 29770; Mature: 29639

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELEEDDAKLRSGIQSIEVGFKLLQALAASPRAMMLRDLAAAAEMNPAKAHRYLVSFLR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LGAVAQDPVSGRYDLGPFALQLGLAGLNRLDPVKKARPILSQLRDEIDLTAGIAVWGNHG
HCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCC
PTIVHWEESSHPVTVSLRLGDVMPMLNSATGRLYGAYLSRKQTLPLIERELAARGQDDVP
CEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCC
DIPASLSEYDAICADVRTHGAARTRGGVLPGINAFSMPVFDANGHLAMGLIVLGAQSIFD
CCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHC
AEWNGTVDRRVREIAKQLSSELGYLGAAQTAGRNGPL
CCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCC
>Mature Secondary Structure 
TELEEDDAKLRSGIQSIEVGFKLLQALAASPRAMMLRDLAAAAEMNPAKAHRYLVSFLR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LGAVAQDPVSGRYDLGPFALQLGLAGLNRLDPVKKARPILSQLRDEIDLTAGIAVWGNHG
HCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCC
PTIVHWEESSHPVTVSLRLGDVMPMLNSATGRLYGAYLSRKQTLPLIERELAARGQDDVP
CEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCC
DIPASLSEYDAICADVRTHGAARTRGGVLPGINAFSMPVFDANGHLAMGLIVLGAQSIFD
CCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHC
AEWNGTVDRRVREIAKQLSSELGYLGAAQTAGRNGPL
CCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]