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The map label for this gene is lipA [H]

Identifier: 73539792

GI number: 73539792

Start: 105147

End: 106142

Strand: Direct

Name: lipA [H]

Synonym: Reut_A0086

Alternate gene names: 73539792

Gene position: 105147-106142 (Clockwise)

Preceding gene: 161611266

Following gene: 73539793

Centisome position: 2.76

GC content: 65.16

Gene sequence:

>996_bases
ATGAGCGACGCCCTGATCGCCTCCTCCAGCGAAGCCCCCCAATCCCCCGCGGAGCAGTACGATCCGACCCGCAAGCAGAA
ATCGGCCGACAAGACCGCGCGCATCCCCATCAAGATCGTGCCGGCCGAAAAGCTCAAGAAGCCGGACTGGATCCGCGTGA
AGGCCGCCACCGGCAATTCGCGTTTCTACGAGATCAAGGACATCCTGCGCGCGAACAACCTCGTCACGGTGTGCGAGGAA
GCAAGCTGCCCGAACATCGGCGAATGCTTCGGCAAGGGCACGGCGACGTTCATGATCATGGGCGACAAGTGCACGCGCCG
GTGCCCGTTCTGCGACGTCGGCCACGGCCGCCCCGATCCGCTCGATGTGAACGAGCCCGGCAACCTGGCCCGCACCATCG
CCCAACTCAAGCTCAACTACGTGGTCATCACCAGCGTTGACCGTGACGACCTGCGCGACGGCGGTGCCCAGCACTACGTG
GACTGCATCAGCCAGACGCGCGAGCTGTCGCCCGCCACCCGCATCGAAGTGCTGGTGCCCGACTTCCGCGGCCGCCTGGA
CAAGGCGCTGGACATCCTGCAGGCCTGCCCGCCGGACGTCATGAACCACAACATGGAAACGGTGCCGCGCCTGTACAAGC
AGGCCCGCCCGGGTGCCGACTACGCGCACTCGCTCAAGCTGCTGCAGGAATTCAAGCGCCGCAACCCGAACGTGCCGACC
AAGTCCGGCCTGATGGTCGGCCTGGGCGAGACCGACGAGGAAATCCTCGAAGTCATGCGCGACATGCGCGCGCACGATAT
CGACATGCTGACCATCGGCCAGTACCTCGCACCGTCGAACCACCACCTGCCGGTGCTGCGCTACGTGCATCCCGATACGT
TCAAGATGTTCGAGGAAGAGGCCTACAAGATGGGCTTCACCCACGCCGCCGTGGGCGCGATGGTGCGCAGCTCGTACCAC
GCCGACCAGCAGGCGCATCAGGCGGGGTTTGCCTGA

Upstream 100 bases:

>100_bases
CGTGCGCTGGCGGCAGACAAGACTGCCGCCACGCCGGCCCTGGCCTCTTAGTGACAAGCTGACAAGCGAATTGCACGCCG
TTGCGCGTGCGGAGAAATTT

Downstream 100 bases:

>100_bases
TCGACGCTGTGGTGACGAAGTAGGGAAAGACGGCCGGGGAAACCCGGCCGTTTTGCTTTTGGGCCGCCCGCTACAACTGT
CCCGGATGGCGGCCAATGTA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MSDALIASSSEAPQSPAEQYDPTRKQKSADKTARIPIKIVPAEKLKKPDWIRVKAATGNSRFYEIKDILRANNLVTVCEE
ASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDVNEPGNLARTIAQLKLNYVVITSVDRDDLRDGGAQHYV
DCISQTRELSPATRIEVLVPDFRGRLDKALDILQACPPDVMNHNMETVPRLYKQARPGADYAHSLKLLQEFKRRNPNVPT
KSGLMVGLGETDEEILEVMRDMRAHDIDMLTIGQYLAPSNHHLPVLRYVHPDTFKMFEEEAYKMGFTHAAVGAMVRSSYH
ADQQAHQAGFA

Sequences:

>Translated_331_residues
MSDALIASSSEAPQSPAEQYDPTRKQKSADKTARIPIKIVPAEKLKKPDWIRVKAATGNSRFYEIKDILRANNLVTVCEE
ASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDVNEPGNLARTIAQLKLNYVVITSVDRDDLRDGGAQHYV
DCISQTRELSPATRIEVLVPDFRGRLDKALDILQACPPDVMNHNMETVPRLYKQARPGADYAHSLKLLQEFKRRNPNVPT
KSGLMVGLGETDEEILEVMRDMRAHDIDMLTIGQYLAPSNHHLPVLRYVHPDTFKMFEEEAYKMGFTHAAVGAMVRSSYH
ADQQAHQAGFA
>Mature_330_residues
SDALIASSSEAPQSPAEQYDPTRKQKSADKTARIPIKIVPAEKLKKPDWIRVKAATGNSRFYEIKDILRANNLVTVCEEA
SCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDVNEPGNLARTIAQLKLNYVVITSVDRDDLRDGGAQHYVD
CISQTRELSPATRIEVLVPDFRGRLDKALDILQACPPDVMNHNMETVPRLYKQARPGADYAHSLKLLQEFKRRNPNVPTK
SGLMVGLGETDEEILEVMRDMRAHDIDMLTIGQYLAPSNHHLPVLRYVHPDTFKMFEEEAYKMGFTHAAVGAMVRSSYHA
DQQAHQAGFA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=294, Percent_Identity=45.578231292517, Blast_Score=264, Evalue=7e-71,
Organism=Homo sapiens, GI37577164, Length=256, Percent_Identity=46.484375, Blast_Score=231, Evalue=7e-61,
Organism=Escherichia coli, GI1786846, Length=304, Percent_Identity=65.1315789473684, Blast_Score=419, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI32564533, Length=328, Percent_Identity=39.6341463414634, Blast_Score=220, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6324770, Length=291, Percent_Identity=43.9862542955326, Blast_Score=245, Evalue=7e-66,
Organism=Drosophila melanogaster, GI221513272, Length=313, Percent_Identity=44.408945686901, Blast_Score=264, Evalue=6e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 36980; Mature: 36848

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDALIASSSEAPQSPAEQYDPTRKQKSADKTARIPIKIVPAEKLKKPDWIRVKAATGNS
CCCCCCCCCCCCCCCCHHHCCCHHHHHCCCCHHCCCEEEECHHHCCCCCEEEEEEECCCC
RFYEIKDILRANNLVTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDP
CHHHHHHHHHHCCEEEEECCCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCCCCC
LDVNEPGNLARTIAQLKLNYVVITSVDRDDLRDGGAQHYVDCISQTRELSPATRIEVLVP
CCCCCCHHHHHHHHHHHEEEEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEECC
DFRGRLDKALDILQACPPDVMNHNMETVPRLYKQARPGADYAHSLKLLQEFKRRNPNVPT
HHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCC
KSGLMVGLGETDEEILEVMRDMRAHDIDMLTIGQYLAPSNHHLPVLRYVHPDTFKMFEEE
CCCEEEECCCCHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCCCEEEEECCHHHHHHHHH
AYKMGFTHAAVGAMVRSSYHADQQAHQAGFA
HHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDALIASSSEAPQSPAEQYDPTRKQKSADKTARIPIKIVPAEKLKKPDWIRVKAATGNS
CCCCCCCCCCCCCCCHHHCCCHHHHHCCCCHHCCCEEEECHHHCCCCCEEEEEEECCCC
RFYEIKDILRANNLVTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDP
CHHHHHHHHHHCCEEEEECCCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCCCCC
LDVNEPGNLARTIAQLKLNYVVITSVDRDDLRDGGAQHYVDCISQTRELSPATRIEVLVP
CCCCCCHHHHHHHHHHHEEEEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEECC
DFRGRLDKALDILQACPPDVMNHNMETVPRLYKQARPGADYAHSLKLLQEFKRRNPNVPT
HHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCC
KSGLMVGLGETDEEILEVMRDMRAHDIDMLTIGQYLAPSNHHLPVLRYVHPDTFKMFEEE
CCCEEEECCCCHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCCCEEEEECCHHHHHHHHH
AYKMGFTHAAVGAMVRSSYHADQQAHQAGFA
HHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA