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The map label for this gene is gcvA [H]

Identifier: 73539789

GI number: 73539789

Start: 102694

End: 103623

Strand: Reverse

Name: gcvA [H]

Synonym: Reut_A0083

Alternate gene names: 73539789

Gene position: 103623-102694 (Counterclockwise)

Preceding gene: 73539803

Following gene: 161611267

Centisome position: 2.72

GC content: 65.91

Gene sequence:

>930_bases
ATGCCGCGCGGCTGGCACCGTGAATTGCCGCGTCTGCCGGCACTGACGGCATTGCGCGCGTTCGAGGCGGCGGCCCGTCA
TGAAAGCTTCTCCCGGGCGGCCACCGAGCTGTTTGTCACGCATGGCGCGGTCAGCCACCAGATCCGCGCGCTCGAAGAAG
AGCTGGGCCTGCCGCTATTCGAGCGCCATGGCAAGCGCGTCGCGCTGACGCCGCCCGGGCGTCTCTACGCCGAGCGGATC
CGCGACGCCCTGCTGCAGATTGCCGACGCAACGCGCGTGCTGCAGTCCGGCAACCGCGACAAGCGCCTGACCATCAGCAC
CATGCCGTCATTCGCCGCGCGCTGGCTAATGCCGCGCATCGGCAGCTTCATTGAACGGCACCCGGAACTCGACGTCGAGC
TGCTGTCGTCGAACACACTCGTGGACTTCGGCCAGGAAGAGGTCGATATCGCGCTGCGCATGGGCACCGGCGACTACCCG
GGCCTTTACGTGGAAAAGCTGCTCGACGACGTGTTCTTCCCGGTCTGCAGCCCGGGGTTCAACGGCGGTCGTCTGCCGGA
GAAGCCCAGCGACCTCGCCGGCCTGAACCTGCTGCGCGGCGAAGGCGATCCATGGAAACCTTGGTTCGAAGCCGCCGGAC
TAGACTGGCCCGAGCCGCGCAAGGGGCTGATGCTCGAAGATTCCTCGCTGCTGCTGCAGGCAGCAGCCGAAGGCCAGGGC
ATTGCGCTGATCCGCTCTTCGTTGGCTTATAACGACCTGTTGTCAGGGCGCGTGGTGCGGCTGTTCGATGTCAGCATTAC
GTGCCCGTGGCTGCTGTACTTCGTATGCGCGCCAGGGGCACTCGAATTGGCAAAGGTGCAGGCGTTCCGCGGGTGGCTGT
TGCCGGAGATCGACCGGTTTCGGGAAGTGCTGGCGCAGTGGGCGGATTGA

Upstream 100 bases:

>100_bases
TGCCCACGCACTGGCAAAACGATATATTTTGGAGGTTCTTGTTAGTGAATCTCACAAAGCGGAGGCCGTCATGGCGTGGA
AAGGCGCATGGGAAAGGGAC

Downstream 100 bases:

>100_bases
GGGTTCAGTCGCCCGACGTCTCGTCATCGTCCGAGCCGTCCTTGGACGGCGCCGCGCGCCCGGCCCTCGGATGCGCCTTG
TCATAGACTTTCGCCAGATG

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MPRGWHRELPRLPALTALRAFEAAARHESFSRAATELFVTHGAVSHQIRALEEELGLPLFERHGKRVALTPPGRLYAERI
RDALLQIADATRVLQSGNRDKRLTISTMPSFAARWLMPRIGSFIERHPELDVELLSSNTLVDFGQEEVDIALRMGTGDYP
GLYVEKLLDDVFFPVCSPGFNGGRLPEKPSDLAGLNLLRGEGDPWKPWFEAAGLDWPEPRKGLMLEDSSLLLQAAAEGQG
IALIRSSLAYNDLLSGRVVRLFDVSITCPWLLYFVCAPGALELAKVQAFRGWLLPEIDRFREVLAQWAD

Sequences:

>Translated_309_residues
MPRGWHRELPRLPALTALRAFEAAARHESFSRAATELFVTHGAVSHQIRALEEELGLPLFERHGKRVALTPPGRLYAERI
RDALLQIADATRVLQSGNRDKRLTISTMPSFAARWLMPRIGSFIERHPELDVELLSSNTLVDFGQEEVDIALRMGTGDYP
GLYVEKLLDDVFFPVCSPGFNGGRLPEKPSDLAGLNLLRGEGDPWKPWFEAAGLDWPEPRKGLMLEDSSLLLQAAAEGQG
IALIRSSLAYNDLLSGRVVRLFDVSITCPWLLYFVCAPGALELAKVQAFRGWLLPEIDRFREVLAQWAD
>Mature_308_residues
PRGWHRELPRLPALTALRAFEAAARHESFSRAATELFVTHGAVSHQIRALEEELGLPLFERHGKRVALTPPGRLYAERIR
DALLQIADATRVLQSGNRDKRLTISTMPSFAARWLMPRIGSFIERHPELDVELLSSNTLVDFGQEEVDIALRMGTGDYPG
LYVEKLLDDVFFPVCSPGFNGGRLPEKPSDLAGLNLLRGEGDPWKPWFEAAGLDWPEPRKGLMLEDSSLLLQAAAEGQGI
ALIRSSLAYNDLLSGRVVRLFDVSITCPWLLYFVCAPGALELAKVQAFRGWLLPEIDRFREVLAQWAD

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=297, Percent_Identity=41.7508417508418, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI1788706, Length=293, Percent_Identity=30.3754266211604, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1786448, Length=290, Percent_Identity=33.1034482758621, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=29.6928327645051, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786401, Length=258, Percent_Identity=28.6821705426357, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI157672245, Length=155, Percent_Identity=32.258064516129, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787128, Length=148, Percent_Identity=33.1081081081081, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI87081978, Length=263, Percent_Identity=27.7566539923954, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790262, Length=236, Percent_Identity=27.5423728813559, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787589, Length=257, Percent_Identity=26.8482490272374, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI145693105, Length=167, Percent_Identity=25.748502994012, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788887, Length=264, Percent_Identity=27.6515151515151, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34565; Mature: 34434

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRGWHRELPRLPALTALRAFEAAARHESFSRAATELFVTHGAVSHQIRALEEELGLPLF
CCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ERHGKRVALTPPGRLYAERIRDALLQIADATRVLQSGNRDKRLTISTMPSFAARWLMPRI
HHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHH
GSFIERHPELDVELLSSNTLVDFGQEEVDIALRMGTGDYPGLYVEKLLDDVFFPVCSPGF
HHHHHHCCCCCEEEECCCCEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCC
NGGRLPEKPSDLAGLNLLRGEGDPWKPWFEAAGLDWPEPRKGLMLEDSSLLLQAAAEGQG
CCCCCCCCCCCHHCCHHCCCCCCCCCHHHHHCCCCCCCCCCCEEECCHHHHHHHHCCCCC
IALIRSSLAYNDLLSGRVVRLFDVSITCPWLLYFVCAPGALELAKVQAFRGWLLPEIDRF
HHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHH
REVLAQWAD
HHHHHHHCC
>Mature Secondary Structure 
PRGWHRELPRLPALTALRAFEAAARHESFSRAATELFVTHGAVSHQIRALEEELGLPLF
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ERHGKRVALTPPGRLYAERIRDALLQIADATRVLQSGNRDKRLTISTMPSFAARWLMPRI
HHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHH
GSFIERHPELDVELLSSNTLVDFGQEEVDIALRMGTGDYPGLYVEKLLDDVFFPVCSPGF
HHHHHHCCCCCEEEECCCCEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCC
NGGRLPEKPSDLAGLNLLRGEGDPWKPWFEAAGLDWPEPRKGLMLEDSSLLLQAAAEGQG
CCCCCCCCCCCHHCCHHCCCCCCCCCHHHHHCCCCCCCCCCCEEECCHHHHHHHHCCCCC
IALIRSSLAYNDLLSGRVVRLFDVSITCPWLLYFVCAPGALELAKVQAFRGWLLPEIDRF
HHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHH
REVLAQWAD
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]