Click here to switch to the map view.

The map label for this gene is lipB [H]

Identifier: 161611266

GI number: 161611266

Start: 104387

End: 105097

Strand: Direct

Name: lipB [H]

Synonym: Reut_A0085

Alternate gene names: 161611266

Gene position: 104387-105097 (Clockwise)

Preceding gene: 73539790

Following gene: 73539792

Centisome position: 2.74

GC content: 66.53

Gene sequence:

>711_bases
ATGCATCCGATTCAAGTCATCGAACGGGGCCGAGAAGACTACCAGCCCTGCTTCGACGCGATGCGCGCCTTCACTGTCGC
GCGCACGCCTGAAACGCCTGACCAGGTCTGGCTGGTCGAGCATCCGCCGGTCTACACGCTGGGCCAGGCGGGCGATCCCG
CACACCTGCTGGCGCCGGACGAACGCATCCCGATGGTGCAGATCGACCGTGGCGGACAGATCACCTATCACGGTCCTGGC
CAGGTGGTGGCCTATCTGCTGCTCGATCTCAAGCGCCGCAAGCTGATGGTGCGCGAGCTCGTCAACGATATCGAGCAGGC
CGTTCTTGACACACTCGCGGCGTATAATCTCGCGGCTGAGCGCAAGCCCGGCGCCCCTGGCATCTACCTGTCGGACGGCC
CGCACCGGGGTGCCAAGATTGCGGCGCTCGGCCTCAAGATCCGCAACGGCTGCAGCTACCACGGCGTCAGCCTGAACGTG
CAGATGGATCTGGCGCCGTTCCTGCGCATCAACCCGTGTGGCTATGCCGGACTGGAAACGGTCGACATGGCCACCGCCGG
TGGGCACTGGCAAGACCAGCCCGTGACCGCCGCACAACAACCTGACATCGCGCGGCGCCTGGCGGCGGCATTGTGCGAGG
TGCTGGCAGCGCGCGAGGCTCGTGCGCTGGCGGCAGACAAGACTGCCGCCACGCCGGCCCTGGCCTCTTAG

Upstream 100 bases:

>100_bases
GTGATGAAGAAGGCCGCCAGGACGCGCAGCACAGGCTGAAGGACGCTCCATGACCGGGGCAGGGCCGGCGCAAGCCGGCC
CTTTTCGTTACACTCTCGCC

Downstream 100 bases:

>100_bases
TGACAAGCTGACAAGCGAATTGCACGCCGTTGCGCGTGCGGAGAAATTTATGAGCGACGCCCTGATCGCCTCCTCCAGCG
AAGCCCCCCAATCCCCCGCG

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MHPIQVIERGREDYQPCFDAMRAFTVARTPETPDQVWLVEHPPVYTLGQAGDPAHLLAPDERIPMVQIDRGGQITYHGPG
QVVAYLLLDLKRRKLMVRELVNDIEQAVLDTLAAYNLAAERKPGAPGIYLSDGPHRGAKIAALGLKIRNGCSYHGVSLNV
QMDLAPFLRINPCGYAGLETVDMATAGGHWQDQPVTAAQQPDIARRLAAALCEVLAAREARALAADKTAATPALAS

Sequences:

>Translated_236_residues
MHPIQVIERGREDYQPCFDAMRAFTVARTPETPDQVWLVEHPPVYTLGQAGDPAHLLAPDERIPMVQIDRGGQITYHGPG
QVVAYLLLDLKRRKLMVRELVNDIEQAVLDTLAAYNLAAERKPGAPGIYLSDGPHRGAKIAALGLKIRNGCSYHGVSLNV
QMDLAPFLRINPCGYAGLETVDMATAGGHWQDQPVTAAQQPDIARRLAAALCEVLAAREARALAADKTAATPALAS
>Mature_236_residues
MHPIQVIERGREDYQPCFDAMRAFTVARTPETPDQVWLVEHPPVYTLGQAGDPAHLLAPDERIPMVQIDRGGQITYHGPG
QVVAYLLLDLKRRKLMVRELVNDIEQAVLDTLAAYNLAAERKPGAPGIYLSDGPHRGAKIAALGLKIRNGCSYHGVSLNV
QMDLAPFLRINPCGYAGLETVDMATAGGHWQDQPVTAAQQPDIARRLAAALCEVLAAREARALAADKTAATPALAS

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Escherichia coli, GI87081767, Length=208, Percent_Identity=50, Blast_Score=204, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6323268, Length=172, Percent_Identity=30.8139534883721, Blast_Score=83, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21355483, Length=187, Percent_Identity=31.0160427807487, Blast_Score=77, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 25559; Mature: 25559

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPIQVIERGREDYQPCFDAMRAFTVARTPETPDQVWLVEHPPVYTLGQAGDPAHLLAPD
CCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEECCCCCCCEECCCC
ERIPMVQIDRGGQITYHGPGQVVAYLLLDLKRRKLMVRELVNDIEQAVLDTLAAYNLAAE
CCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RKPGAPGIYLSDGPHRGAKIAALGLKIRNGCSYHGVSLNVQMDLAPFLRINPCGYAGLET
CCCCCCEEEECCCCCCCCEEEEEEEEECCCCCCCCEEEEEEECCCCCEEECCCCCCCCCH
VDMATAGGHWQDQPVTAAQQPDIARRLAAALCEVLAAREARALAADKTAATPALAS
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MHPIQVIERGREDYQPCFDAMRAFTVARTPETPDQVWLVEHPPVYTLGQAGDPAHLLAPD
CCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEECCCCCCCEECCCC
ERIPMVQIDRGGQITYHGPGQVVAYLLLDLKRRKLMVRELVNDIEQAVLDTLAAYNLAAE
CCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RKPGAPGIYLSDGPHRGAKIAALGLKIRNGCSYHGVSLNVQMDLAPFLRINPCGYAGLET
CCCCCCEEEECCCCCCCCEEEEEEEEECCCCCCCCEEEEEEECCCCCEEECCCCCCCCCH
VDMATAGGHWQDQPVTAAQQPDIARRLAAALCEVLAAREARALAADKTAATPALAS
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA