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The map label for this gene is asnB [H]

Identifier: 71911329

GI number: 71911329

Start: 1469318

End: 1470283

Strand: Direct

Name: asnB [H]

Synonym: M5005_Spy_1516

Alternate gene names: 71911329

Gene position: 1469318-1470283 (Clockwise)

Preceding gene: 71911327

Following gene: 71911333

Centisome position: 79.92

GC content: 39.13

Gene sequence:

>966_bases
ATGAAAAAAATCCTAGTCTTACACACTGGTGGAACCATTTCTATGCAAGCTGATAACTCTGGTAGGGTTGTTCCAAATCA
AGATAATCCTATGACGAAAATACATGCTGCAGCCCAAGATATTCAGCTTACCGTTAGTGATTTTCTAAATCTTCCGAGTC
CTCACATCACGCCTCACCACATGTTATCTATTTATCATCATATTCAAGAAAGAACTGATGTCTTTGATGGTATAGTCATC
ACACATGGAACAGATACCTTAGAAGAAACAGCTTATTTTTTAGATACCATGGCATTGCCTACTAATATACCTGTTGTCCT
AACAGGTGCTATGAGGTCTTCTAATGAAGTTGGTAGTGATGGCATATATAATTACCTTACCGCACTTAGAGTTGCAAGTA
GTGATAAAGCTAAAGAAAAAGGGGTACTTGTTGTTATGAACGATGAAATCCATGCTGCTAAATATGTTACTAAAACACAT
ACAACCAATATTTCAACTTTCCAAACACCCACACACGGACCGCTTGGTATCATCATGAAAAATGACCTACTTTTTTTCAA
AACTGCTGAACCTCGTATTCGTTTTGACTTGAGATGCATTTCTGGAACCATCCCCATTATCAAAGCATACGCAGGTATGG
GCGATGGTAGTATCTTGAGTCTATTAACTCCAGGTAGCATTCAAGGTCTAGTTATCGAAGCTCTAGGGGCAGGAAATGTC
CCACCATTAGCAGTTGGGGAGATCGAGCACTTAATTACTTTAGGTATTCCTGTCATTCTTGTTTCCCGTTGTTTCAATGG
TATGGCAGAACCTGTCTATGCTTATGAAGGTGGTGGTGCGATGCTACAAGAAGCAGGTGTTATGTTTGTAAAAGAATTGA
ATGCTCCAAAAGCCCGTCTAAAGTTATTAATTGCTCTCAACGCCGGACTTACTGGTCAAGAGCTAAAAGATTATATTGAA
GGATAA

Upstream 100 bases:

>100_bases
ATATCAAAAAAGACTGCTTTTACTGTCAAAACCTTCTTTTGCCTCCTGTGTTTATGATAGAATAGATTATATCATAAATA
AAAGGAAGTTTGACAATCAA

Downstream 100 bases:

>100_bases
TCTAGCTTCCTTTAGAAATGTCCCACTAAAACATATTAGTGGGACATTTCTAGTACTTTAAGCTTTAAATTAGGACATCT
CCTAAATGTCTAGAATAAAC

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKKILVLHTGGTISMQADNSGRVVPNQDNPMTKIHAAAQDIQLTVSDFLNLPSPHITPHHMLSIYHHIQERTDVFDGIVI
THGTDTLEETAYFLDTMALPTNIPVVLTGAMRSSNEVGSDGIYNYLTALRVASSDKAKEKGVLVVMNDEIHAAKYVTKTH
TTNISTFQTPTHGPLGIIMKNDLLFFKTAEPRIRFDLRCISGTIPIIKAYAGMGDGSILSLLTPGSIQGLVIEALGAGNV
PPLAVGEIEHLITLGIPVILVSRCFNGMAEPVYAYEGGGAMLQEAGVMFVKELNAPKARLKLLIALNAGLTGQELKDYIE
G

Sequences:

>Translated_321_residues
MKKILVLHTGGTISMQADNSGRVVPNQDNPMTKIHAAAQDIQLTVSDFLNLPSPHITPHHMLSIYHHIQERTDVFDGIVI
THGTDTLEETAYFLDTMALPTNIPVVLTGAMRSSNEVGSDGIYNYLTALRVASSDKAKEKGVLVVMNDEIHAAKYVTKTH
TTNISTFQTPTHGPLGIIMKNDLLFFKTAEPRIRFDLRCISGTIPIIKAYAGMGDGSILSLLTPGSIQGLVIEALGAGNV
PPLAVGEIEHLITLGIPVILVSRCFNGMAEPVYAYEGGGAMLQEAGVMFVKELNAPKARLKLLIALNAGLTGQELKDYIE
G
>Mature_321_residues
MKKILVLHTGGTISMQADNSGRVVPNQDNPMTKIHAAAQDIQLTVSDFLNLPSPHITPHHMLSIYHHIQERTDVFDGIVI
THGTDTLEETAYFLDTMALPTNIPVVLTGAMRSSNEVGSDGIYNYLTALRVASSDKAKEKGVLVVMNDEIHAAKYVTKTH
TTNISTFQTPTHGPLGIIMKNDLLFFKTAEPRIRFDLRCISGTIPIIKAYAGMGDGSILSLLTPGSIQGLVIEALGAGNV
PPLAVGEIEHLITLGIPVILVSRCFNGMAEPVYAYEGGGAMLQEAGVMFVKELNAPKARLKLLIALNAGLTGQELKDYIE
G

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=276, Percent_Identity=32.2463768115942, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1788065, Length=329, Percent_Identity=27.0516717325228, Blast_Score=82, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17558280, Length=270, Percent_Identity=25.5555555555556, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17558278, Length=270, Percent_Identity=25.5555555555556, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193207374, Length=270, Percent_Identity=25.5555555555556, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193207372, Length=270, Percent_Identity=25.5555555555556, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320527, Length=334, Percent_Identity=30.2395209580838, Blast_Score=132, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6323189, Length=345, Percent_Identity=28.695652173913, Blast_Score=115, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6323187, Length=345, Percent_Identity=28.695652173913, Blast_Score=115, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6323186, Length=345, Percent_Identity=28.695652173913, Blast_Score=115, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6323184, Length=345, Percent_Identity=28.695652173913, Blast_Score=115, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24645530, Length=378, Percent_Identity=25.3968253968254, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24639609, Length=246, Percent_Identity=26.4227642276423, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 34595; Mature: 34595

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS01076 ACETATE_KINASE_2 ; PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILVLHTGGTISMQADNSGRVVPNQDNPMTKIHAAAQDIQLTVSDFLNLPSPHITPHH
CCEEEEEECCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHEEEEHHHHHCCCCCCCCHHH
MLSIYHHIQERTDVFDGIVITHGTDTLEETAYFLDTMALPTNIPVVLTGAMRSSNEVGSD
HHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCC
GIYNYLTALRVASSDKAKEKGVLVVMNDEIHAAKYVTKTHTTNISTFQTPTHGPLGIIMK
HHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHEEEEECCCCCEEECCCCCCCEEEEEE
NDLLFFKTAEPRIRFDLRCISGTIPIIKAYAGMGDGSILSLLTPGSIQGLVIEALGAGNV
CCEEEEEECCCCEEEEEEEECCCCHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCC
PPLAVGEIEHLITLGIPVILVSRCFNGMAEPVYAYEGGGAMLQEAGVMFVKELNAPKARL
CCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHCCEEEEHHCCCCHHHE
KLLIALNAGLTGQELKDYIEG
EEEEEECCCCCHHHHHHHHCC
>Mature Secondary Structure
MKKILVLHTGGTISMQADNSGRVVPNQDNPMTKIHAAAQDIQLTVSDFLNLPSPHITPHH
CCEEEEEECCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHEEEEHHHHHCCCCCCCCHHH
MLSIYHHIQERTDVFDGIVITHGTDTLEETAYFLDTMALPTNIPVVLTGAMRSSNEVGSD
HHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCC
GIYNYLTALRVASSDKAKEKGVLVVMNDEIHAAKYVTKTHTTNISTFQTPTHGPLGIIMK
HHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHEEEEECCCCCEEECCCCCCCEEEEEE
NDLLFFKTAEPRIRFDLRCISGTIPIIKAYAGMGDGSILSLLTPGSIQGLVIEALGAGNV
CCEEEEEECCCCEEEEEEEECCCCHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCC
PPLAVGEIEHLITLGIPVILVSRCFNGMAEPVYAYEGGGAMLQEAGVMFVKELNAPKARL
CCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHCCEEEEHHCCCCHHHE
KLLIALNAGLTGQELKDYIEG
EEEEEECCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]