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The map label for this gene is kup [H]

Identifier: 71910966

GI number: 71910966

Start: 1129059

End: 1130123

Strand: Reverse

Name: kup [H]

Synonym: M5005_Spy_1153

Alternate gene names: 71910966

Gene position: 1130123-1129059 (Counterclockwise)

Preceding gene: 71910967

Following gene: 71910965

Centisome position: 61.47

GC content: 39.53

Gene sequence:

>1065_bases
ATGTCTGATAGCCATCTTACTGCCTTTGACAAAGCTTCAAAAGCAGGTTTTATTATTGCTTTAGGTATTGTTTATGGAGA
TATTGGAACAAGCCCCCTTTATACGATACAGTCTCTAGTTGAAAATCAAGGAGGGGTTAATCAGGTTTCTGAATCCTTTA
TTTTAGGATCAATCTCTTTAATTATTTGGACACTGACCTTAATCACAACAATCAAGTACGTTTTGATTGCTCTAAAAGCG
GATAACCACCATGAAGGTGGGATTTTTTCTTTATTCACCTTGGTACGTAAAATGTCACCGTGGCTCATTATTCCGGCGAT
GATTGGAGGTGCAACACTTTTGTCTGATGGGGCTCTTACACCTGCGGTAACAGTTACTTCAGCTATTGAGGGCTTGAAAG
CCGTTCCTGGTCTGAGTCACATCTACCAAAATCAAACCAATGTCATTATCACAACTCTAGTGATTTTAATAGTCTTGTTT
GGTATTCAGCGGTTTGGAACAGGTTTTATTGGTAAAATTTTTGGACCAGTTATGTTTATCTGGTTTAGTTTTCTAGGTGT
TAGTGGTTTTTTTAATACGTTGGGGCATCTGGAAATTTTCAAGGCTATCAATCCTTACTATGCTCTGCATTTGCTGTTTA
GTCCTGAAAACCATCGTGGGATTTTTATCTTGGGGTCCATTTTTCTTGCGACAACAGGAGCTGAAGCTCTTTATTCGGAT
TTAGGACATGTTGGTCGAGGAAATATTTACGTGAGCTGGCCCTTTGTTAAGATGTGTATTGTGTTGTCTTACTGTGGCCA
GGCTGCGTGGATTTTAGCGAATAAACACTCAGGTATTGAACTAAACCCTTTCTTTGCTAGTGTGCCATCACAATTAAGAG
TTTACCTTGTTAGTTTGGCAACCTTGGCAGCTATTATTGCCTCACAGGCTCTTATTTCAGGCTCCTTTACCTTGGTTTCA
GAGGCCATGCGTTTGAAAATTTTCCCTTTGTTTCGTGTGACTTACCCAGGGCAAATTTGGGGCAATTGTATATTCCTGTC
ATTAACTGGATTTTATTTGCAGTGA

Upstream 100 bases:

>100_bases
TCTTTTTAATTTTCTGAAAAAGTTGCTTTTATAATGGAATCTGTTATAATGGTTTCAGTTATTTTTGTGATAATTTTTAA
AAAATTAGGAGGAGTATAAG

Downstream 100 bases:

>100_bases
CCTCTTGTACTGTTTTGGCTTTTAGAACATCAGCCCACATGGAGGCAGCTTATGGTCTTGCTATTACCATCACCATGTTG
ATGACAACAATCCTCTTAAA

Product: kup system potassium uptake protein

Products: K (I) [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MSDSHLTAFDKASKAGFIIALGIVYGDIGTSPLYTIQSLVENQGGVNQVSESFILGSISLIIWTLTLITTIKYVLIALKA
DNHHEGGIFSLFTLVRKMSPWLIIPAMIGGATLLSDGALTPAVTVTSAIEGLKAVPGLSHIYQNQTNVIITTLVILIVLF
GIQRFGTGFIGKIFGPVMFIWFSFLGVSGFFNTLGHLEIFKAINPYYALHLLFSPENHRGIFILGSIFLATTGAEALYSD
LGHVGRGNIYVSWPFVKMCIVLSYCGQAAWILANKHSGIELNPFFASVPSQLRVYLVSLATLAAIIASQALISGSFTLVS
EAMRLKIFPLFRVTYPGQIWGNCIFLSLTGFYLQ

Sequences:

>Translated_354_residues
MSDSHLTAFDKASKAGFIIALGIVYGDIGTSPLYTIQSLVENQGGVNQVSESFILGSISLIIWTLTLITTIKYVLIALKA
DNHHEGGIFSLFTLVRKMSPWLIIPAMIGGATLLSDGALTPAVTVTSAIEGLKAVPGLSHIYQNQTNVIITTLVILIVLF
GIQRFGTGFIGKIFGPVMFIWFSFLGVSGFFNTLGHLEIFKAINPYYALHLLFSPENHRGIFILGSIFLATTGAEALYSD
LGHVGRGNIYVSWPFVKMCIVLSYCGQAAWILANKHSGIELNPFFASVPSQLRVYLVSLATLAAIIASQALISGSFTLVS
EAMRLKIFPLFRVTYPGQIWGNCIFLSLTGFYLQ
>Mature_353_residues
SDSHLTAFDKASKAGFIIALGIVYGDIGTSPLYTIQSLVENQGGVNQVSESFILGSISLIIWTLTLITTIKYVLIALKAD
NHHEGGIFSLFTLVRKMSPWLIIPAMIGGATLLSDGALTPAVTVTSAIEGLKAVPGLSHIYQNQTNVIITTLVILIVLFG
IQRFGTGFIGKIFGPVMFIWFSFLGVSGFFNTLGHLEIFKAINPYYALHLLFSPENHRGIFILGSIFLATTGAEALYSDL
GHVGRGNIYVSWPFVKMCIVLSYCGQAAWILANKHSGIELNPFFASVPSQLRVYLVSLATLAAIIASQALISGSFTLVSE
AMRLKIFPLFRVTYPGQIWGNCIFLSLTGFYLQ

Specific function: Transport of potassium into the cell [H]

COG id: COG3158

COG function: function code P; K+ transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAK/KUP transporter (TC 2.A.72) family [H]

Homologues:

Organism=Escherichia coli, GI48994960, Length=341, Percent_Identity=37.2434017595308, Blast_Score=204, Evalue=5e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003855
- InterPro:   IPR023051 [H]

Pfam domain/function: PF02705 K_trans [H]

EC number: NA

Molecular weight: Translated: 38457; Mature: 38326

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSHLTAFDKASKAGFIIALGIVYGDIGTSPLYTIQSLVENQGGVNQVSESFILGSISL
CCCCCCCHHHCCCCCCEEEEEEEHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IIWTLTLITTIKYVLIALKADNHHEGGIFSLFTLVRKMSPWLIIPAMIGGATLLSDGALT
HHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHCCCCEEEHHHHCCHHHCCCCCCC
PAVTVTSAIEGLKAVPGLSHIYQNQTNVIITTLVILIVLFGIQRFGTGFIGKIFGPVMFI
HHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
WFSFLGVSGFFNTLGHLEIFKAINPYYALHLLFSPENHRGIFILGSIFLATTGAEALYSD
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCEEEEEEHHHHHCCHHHHHHH
LGHVGRGNIYVSWPFVKMCIVLSYCGQAAWILANKHSGIELNPFFASVPSQLRVYLVSLA
HCCCCCCCEEEECHHHHHHHHHHHCCCEEEEEEECCCCEEECHHHHHCHHHHHHHHHHHH
TLAAIIASQALISGSFTLVSEAMRLKIFPLFRVTYPGQIWGNCIFLSLTGFYLQ
HHHHHHHHHHHHCCCHHHHHHHHHEEEEEEEEECCCCHHHHCEEEEEECCHHCC
>Mature Secondary Structure 
SDSHLTAFDKASKAGFIIALGIVYGDIGTSPLYTIQSLVENQGGVNQVSESFILGSISL
CCCCCCHHHCCCCCCEEEEEEEHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IIWTLTLITTIKYVLIALKADNHHEGGIFSLFTLVRKMSPWLIIPAMIGGATLLSDGALT
HHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHCCCCEEEHHHHCCHHHCCCCCCC
PAVTVTSAIEGLKAVPGLSHIYQNQTNVIITTLVILIVLFGIQRFGTGFIGKIFGPVMFI
HHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
WFSFLGVSGFFNTLGHLEIFKAINPYYALHLLFSPENHRGIFILGSIFLATTGAEALYSD
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCEEEEEEHHHHHCCHHHHHHH
LGHVGRGNIYVSWPFVKMCIVLSYCGQAAWILANKHSGIELNPFFASVPSQLRVYLVSLA
HCCCCCCCEEEECHHHHHHHHHHHCCCEEEEEEECCCCEEECHHHHHCHHHHHHHHHHHH
TLAAIIASQALISGSFTLVSEAMRLKIFPLFRVTYPGQIWGNCIFLSLTGFYLQ
HHHHHHHHHHHHCCCHHHHHHHHHEEEEEEEEECCCCHHHHCEEEEEECCHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: K (I) [Periplasm] [C]

Specific reaction: K (I) [Periplasm] = K (I) [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]