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The map label for this gene is celB [H]

Identifier: 71910892

GI number: 71910892

Start: 1052966

End: 1054345

Strand: Reverse

Name: celB [H]

Synonym: M5005_Spy_1079

Alternate gene names: 71910892

Gene position: 1054345-1052966 (Counterclockwise)

Preceding gene: 71910893

Following gene: 71910891

Centisome position: 57.35

GC content: 40.07

Gene sequence:

>1380_bases
ATGTCGCATTTTTCTGATAAGTTTATGGAGATTTCAGGAAAAATTGGGTCACAACGGCATTTAGTAGCTATTCGTGATTC
CTTTATTTCAATGATGCCTATTACCATGGCAGGTTCAGTTGCTGTCTTATTGAACGTTTTTTTACGCGATATTCCAAACA
ATATGGGATGGACAGGATTTGCAAAAGCTATGCAACCTGTTATCGACATCAATGGCTATGTTTATTTTGGGACAATTGGT
ATCATGGCGCTCTTTTTTGCCTTTGCTTTCGGTTACAATTTAGCTGTTATGCATAAGATTAATCCATTGGCAGGAGGATT
GATTTCTTTTGGCTCATTTATTGCCACCCTACCTCAGACTTTAACCATTTCGACACCACTTGAAAATGCCTCTGCTAACC
TAATTAGCAATTTGAAAGAAATGGGGCTTTCAGTCGTTACTGCGGGTGGAGCTTCAAGTATTGAAACTAGTCAATGGGGA
GCAATAGCTCTAAAGTATGTCGGTGCCACAGGCTTGTTCACAGCTTTAATTATTGGCTTTTTATCAAGTTTTGTGTATGC
GGCTTTGACCAAACGTAATATTACCATTACCTTACCAGATAATGTACCACCTGCGGTTAATAAAGCCTTTGCTGCCATTA
TTCCAGGAACAGTTGCGATTTACGCTTCGGCAATTTTCGCCTACCTCATTTTCGCATTAACAGGTTCTTCCCTAAGTGAT
GTCATTTCAACCTATATCCAATTACCATTGCTAGGCTTATCACAGGGTATTGGTTCAGTTATCTTGTTAACCTTCTTAGT
TCAACTGCTCTGGTTCTTCGGCTTGCATGGTCATAATGTCTTAGCCCCAGTGATGGATGGTATTTATATGGTAGCTTTAA
CAGAAAATACAGCTGCTTACAATACAGCGCATAGCGCTGCTAATTTACCATACCTTTGGACACGCGGGTCCTTTGATGCT
TATGCTCAAATGGGTGGTTCAGGAGTAACCTTGGCATTGATTATTGCTATCTTCATATTCTCAAAACGAGAAGAGCATAA
AACCATTGCCAAATTGTCCGCACCAATGGGTGTTTTCAATATCAACGAACCAATTACATTTGGGATGCCAATTGTTTTGA
ATCCAACCTTTGTGATTCCATGGCTAATTGTGCCACCAATTTGTGCTAGCATAGCTTATTTTGCTACTGCAATAGGTTTG
ATTCCACCGGTATTCTTATCAGTACCTTGGATTACCCCTGTTGGACTTTATGCTTACCTAGCTACTGGAGGTAATATTAT
GGCAGGTTTGGTTTCACTGTTTAACCTCTTTGTTGCCTTCCTTATCTGGGCACCATTTGTCATTTTGGCCAATAAAGAAA
AAGCGAGTGACCTTGCATGA

Upstream 100 bases:

>100_bases
CGTTTAACAAGGATGATTTTGAGATAATTGTGTTAGGCAGACACCGCTGCTTTTAAGGTTACCTTTGTTACATTAAAAAA
ATAAAAAGAGGAGAAGATTT

Downstream 100 bases:

>100_bases
CAGAAAAAAATGGAGCAACTTTTTTAATGGCTGTTGGCTTTTTTCTCATTGCCATTAGTCCGATGGTTGGTAACAAATAC
TTACACATAGTGACGGGACT

Product: PTS system, cellobiose-specific IIC component

Products: pyruvate; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EIIC-Cel; PTS system cellobiose-specific EIIC component [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSHFSDKFMEISGKIGSQRHLVAIRDSFISMMPITMAGSVAVLLNVFLRDIPNNMGWTGFAKAMQPVIDINGYVYFGTIG
IMALFFAFAFGYNLAVMHKINPLAGGLISFGSFIATLPQTLTISTPLENASANLISNLKEMGLSVVTAGGASSIETSQWG
AIALKYVGATGLFTALIIGFLSSFVYAALTKRNITITLPDNVPPAVNKAFAAIIPGTVAIYASAIFAYLIFALTGSSLSD
VISTYIQLPLLGLSQGIGSVILLTFLVQLLWFFGLHGHNVLAPVMDGIYMVALTENTAAYNTAHSAANLPYLWTRGSFDA
YAQMGGSGVTLALIIAIFIFSKREEHKTIAKLSAPMGVFNINEPITFGMPIVLNPTFVIPWLIVPPICASIAYFATAIGL
IPPVFLSVPWITPVGLYAYLATGGNIMAGLVSLFNLFVAFLIWAPFVILANKEKASDLA

Sequences:

>Translated_459_residues
MSHFSDKFMEISGKIGSQRHLVAIRDSFISMMPITMAGSVAVLLNVFLRDIPNNMGWTGFAKAMQPVIDINGYVYFGTIG
IMALFFAFAFGYNLAVMHKINPLAGGLISFGSFIATLPQTLTISTPLENASANLISNLKEMGLSVVTAGGASSIETSQWG
AIALKYVGATGLFTALIIGFLSSFVYAALTKRNITITLPDNVPPAVNKAFAAIIPGTVAIYASAIFAYLIFALTGSSLSD
VISTYIQLPLLGLSQGIGSVILLTFLVQLLWFFGLHGHNVLAPVMDGIYMVALTENTAAYNTAHSAANLPYLWTRGSFDA
YAQMGGSGVTLALIIAIFIFSKREEHKTIAKLSAPMGVFNINEPITFGMPIVLNPTFVIPWLIVPPICASIAYFATAIGL
IPPVFLSVPWITPVGLYAYLATGGNIMAGLVSLFNLFVAFLIWAPFVILANKEKASDLA
>Mature_458_residues
SHFSDKFMEISGKIGSQRHLVAIRDSFISMMPITMAGSVAVLLNVFLRDIPNNMGWTGFAKAMQPVIDINGYVYFGTIGI
MALFFAFAFGYNLAVMHKINPLAGGLISFGSFIATLPQTLTISTPLENASANLISNLKEMGLSVVTAGGASSIETSQWGA
IALKYVGATGLFTALIIGFLSSFVYAALTKRNITITLPDNVPPAVNKAFAAIIPGTVAIYASAIFAYLIFALTGSSLSDV
ISTYIQLPLLGLSQGIGSVILLTFLVQLLWFFGLHGHNVLAPVMDGIYMVALTENTAAYNTAHSAANLPYLWTRGSFDAY
AQMGGSGVTLALIIAIFIFSKREEHKTIAKLSAPMGVFNINEPITFGMPIVLNPTFVIPWLIVPPICASIAYFATAIGLI
PPVFLSVPWITPVGLYAYLATGGNIMAGLVSLFNLFVAFLIWAPFVILANKEKASDLA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=460, Percent_Identity=35, Blast_Score=246, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003352
- InterPro:   IPR004796
- InterPro:   IPR004501 [H]

Pfam domain/function: PF02378 PTS_EIIC [H]

EC number: NA

Molecular weight: Translated: 49025; Mature: 48894

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHFSDKFMEISGKIGSQRHLVAIRDSFISMMPITMAGSVAVLLNVFLRDIPNNMGWTGF
CCCHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
AKAMQPVIDINGYVYFGTIGIMALFFAFAFGYNLAVMHKINPLAGGLISFGSFIATLPQT
HHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCC
LTISTPLENASANLISNLKEMGLSVVTAGGASSIETSQWGAIALKYVGATGLFTALIIGF
EEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHCHHHHHHHHHHHH
LSSFVYAALTKRNITITLPDNVPPAVNKAFAAIIPGTVAIYASAIFAYLIFALTGSSLSD
HHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHH
VISTYIQLPLLGLSQGIGSVILLTFLVQLLWFFGLHGHNVLAPVMDGIYMVALTENTAAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEEEEECCCCHH
NTAHSAANLPYLWTRGSFDAYAQMGGSGVTLALIIAIFIFSKREEHKTIAKLSAPMGVFN
CCCHHCCCCCEEEECCCHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEE
INEPITFGMPIVLNPTFVIPWLIVPPICASIAYFATAIGLIPPVFLSVPWITPVGLYAYL
CCCCCEECCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
ATGGNIMAGLVSLFNLFVAFLIWAPFVILANKEKASDLA
HCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCHHCCCCC
>Mature Secondary Structure 
SHFSDKFMEISGKIGSQRHLVAIRDSFISMMPITMAGSVAVLLNVFLRDIPNNMGWTGF
CCHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
AKAMQPVIDINGYVYFGTIGIMALFFAFAFGYNLAVMHKINPLAGGLISFGSFIATLPQT
HHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCC
LTISTPLENASANLISNLKEMGLSVVTAGGASSIETSQWGAIALKYVGATGLFTALIIGF
EEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHCHHHHHHHHHHHH
LSSFVYAALTKRNITITLPDNVPPAVNKAFAAIIPGTVAIYASAIFAYLIFALTGSSLSD
HHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHH
VISTYIQLPLLGLSQGIGSVILLTFLVQLLWFFGLHGHNVLAPVMDGIYMVALTENTAAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEEEEECCCCHH
NTAHSAANLPYLWTRGSFDAYAQMGGSGVTLALIIAIFIFSKREEHKTIAKLSAPMGVFN
CCCHHCCCCCEEEECCCHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEE
INEPITFGMPIVLNPTFVIPWLIVPPICASIAYFATAIGLIPPVFLSVPWITPVGLYAYL
CCCCCEECCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
ATGGNIMAGLVSLFNLFVAFLIWAPFVILANKEKASDLA
HCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: diacetylchitobiose [Periplasm]; phosphoenolpyruvate; cellobiose [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + diacetylchitobiose [Periplasm] = pyruvate + diacetylchitobiose-6-phosphate [Cytoplasm] phosphoenolpyruvate + cellobiose [Periplasm] = cellobiose-6-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8407820 [H]