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The map label for this gene is lplA [H]

Identifier: 71910741

GI number: 71910741

Start: 918032

End: 919051

Strand: Reverse

Name: lplA [H]

Synonym: M5005_Spy_0928

Alternate gene names: 71910741

Gene position: 919051-918032 (Counterclockwise)

Preceding gene: 71910742

Following gene: 71910740

Centisome position: 49.99

GC content: 38.53

Gene sequence:

>1020_bases
ATGTACCTTATTGAACCGATTCGTAACGGTAAACGTATCACCGATGGAGCTGTTGCTCTAGCCATGCAAGTTTATGTTCA
AGAAAATCTATTTTTAGATGATGATATTTTATTTCCCTACTATTGTGATCCCAAAGTTGAAATTGGAAAATTCCAGAATG
CAGTTGTAGAAACTAATCAAGAGTATTTAAAAGAGCATCATATCCCTGTTGTTCGCAGAGATACTGGCGGTGGTGCCGTT
TATGTAGATTCTGGTGCTGTTAATATTTGCTACTTGATTAATGATAATGGTATTTTTGGTGATTTTAAAAGAACCTATCA
GCCCGCTATTGAAGCCCTTCATCATTTGGGAGCAACAGAGGTTGAAATGTCTGGACGGAATGACTTGGTTATTGATGGGA
AAAAGGTATCTGGCGCTGCGATGACTATTGCTAATGGCCGTGTTTATGGGGGATATTCCCTTTTGCTAGATGTGGACTTT
GAAGCCATGGAAAAAGCATTAAAACCTAATCGCAAAAAAATTGAATCAAAAGGCATCAGGTCAGTTCGTAGTCGCGTTGG
AAACATCAGAGAACATTTAGCACCACAATACCAAGGTATTACGATTGAAGAGTTCAAAGATTTAATGGTTTGCCAATTGT
TACAGATTGAGACGATTAGCCAAGCTAAGCGTTATGATTTAACGGAAAAAGACTGGCAACAAATTGATGCTTTAACAGAG
CGCAAATATCACAATTGGGAATGGAATTATGGCAACGCGCCTCAATACCGTTACCATAGAGATGGCCGTTTTACCGGTGG
AACAGTAGATATTCATTTAGATATTAAAAAAGGCTATATTGCAGCTTGTCGCATTTACGGAGATTTTTTTGGCAAGGCAG
ACATTGCAGAACTTGAAGGTCACTTGATAGGAACACGAATGGAAAAAGAGGATGTCTTAGCTACCCTCAATGCAATTGAT
TTAGCCCCTTATTTAGGTGCGATTACAGCAGAAGAATTAGGAGATTTGATATTTTCTTGA

Upstream 100 bases:

>100_bases
GAACACATTGCACAATTGATTAGCGCCACAAAAACCATTTACCAAAAATCGTAACCAAAGCCAATAGACGGTTAATTAAG
AGATAGAGAAGGAGACTAAC

Downstream 100 bases:

>100_bases
TAAAAAAGATACCTCAGACCGGTAAAGTGTGATTAAAAACGAACAATAATTGTTTTAATTCAAATAAATGTACGTATGAT
ATGAATTGTGATATAATGAC

Product: lipoate-protein ligase A

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MYLIEPIRNGKRITDGAVALAMQVYVQENLFLDDDILFPYYCDPKVEIGKFQNAVVETNQEYLKEHHIPVVRRDTGGGAV
YVDSGAVNICYLINDNGIFGDFKRTYQPAIEALHHLGATEVEMSGRNDLVIDGKKVSGAAMTIANGRVYGGYSLLLDVDF
EAMEKALKPNRKKIESKGIRSVRSRVGNIREHLAPQYQGITIEEFKDLMVCQLLQIETISQAKRYDLTEKDWQQIDALTE
RKYHNWEWNYGNAPQYRYHRDGRFTGGTVDIHLDIKKGYIAACRIYGDFFGKADIAELEGHLIGTRMEKEDVLATLNAID
LAPYLGAITAEELGDLIFS

Sequences:

>Translated_339_residues
MYLIEPIRNGKRITDGAVALAMQVYVQENLFLDDDILFPYYCDPKVEIGKFQNAVVETNQEYLKEHHIPVVRRDTGGGAV
YVDSGAVNICYLINDNGIFGDFKRTYQPAIEALHHLGATEVEMSGRNDLVIDGKKVSGAAMTIANGRVYGGYSLLLDVDF
EAMEKALKPNRKKIESKGIRSVRSRVGNIREHLAPQYQGITIEEFKDLMVCQLLQIETISQAKRYDLTEKDWQQIDALTE
RKYHNWEWNYGNAPQYRYHRDGRFTGGTVDIHLDIKKGYIAACRIYGDFFGKADIAELEGHLIGTRMEKEDVLATLNAID
LAPYLGAITAEELGDLIFS
>Mature_339_residues
MYLIEPIRNGKRITDGAVALAMQVYVQENLFLDDDILFPYYCDPKVEIGKFQNAVVETNQEYLKEHHIPVVRRDTGGGAV
YVDSGAVNICYLINDNGIFGDFKRTYQPAIEALHHLGATEVEMSGRNDLVIDGKKVSGAAMTIANGRVYGGYSLLLDVDF
EAMEKALKPNRKKIESKGIRSVRSRVGNIREHLAPQYQGITIEEFKDLMVCQLLQIETISQAKRYDLTEKDWQQIDALTE
RKYHNWEWNYGNAPQYRYHRDGRFTGGTVDIHLDIKKGYIAACRIYGDFFGKADIAELEGHLIGTRMEKEDVLATLNAID
LAPYLGAITAEELGDLIFS

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=308, Percent_Identity=25.974025974026, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI21729884, Length=308, Percent_Identity=25.974025974026, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI21729882, Length=308, Percent_Identity=25.974025974026, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI21729880, Length=308, Percent_Identity=25.974025974026, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI21729878, Length=308, Percent_Identity=25.974025974026, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1790846, Length=296, Percent_Identity=33.1081081081081, Blast_Score=142, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI71981909, Length=254, Percent_Identity=28.740157480315, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6322415, Length=196, Percent_Identity=31.6326530612245, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28573464, Length=275, Percent_Identity=25.8181818181818, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 38325; Mature: 38325

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLIEPIRNGKRITDGAVALAMQVYVQENLFLDDDILFPYYCDPKVEIGKFQNAVVETNQ
CEEECCCCCCCCCCHHHHHHHHHHHHHCCCEECCCEEEEEECCCCCCHHHHHHHHHHCCH
EYLKEHHIPVVRRDTGGGAVYVDSGAVNICYLINDNGIFGDFKRTYQPAIEALHHLGATE
HHHHHCCCCEEEECCCCCEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCE
VEMSGRNDLVIDGKKVSGAAMTIANGRVYGGYSLLLDVDFEAMEKALKPNRKKIESKGIR
EEECCCCCEEEECCCCCCEEEEEECCEEEECEEEEEECCHHHHHHHHCCCHHHHHHHHHH
SVRSRVGNIREHLAPQYQGITIEEFKDLMVCQLLQIETISQAKRYDLTEKDWQQIDALTE
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
RKYHNWEWNYGNAPQYRYHRDGRFTGGTVDIHLDIKKGYIAACRIYGDFFGKADIAELEG
CCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEEECCCEEEEEEEEHHHCCCCCHHHHCC
HLIGTRMEKEDVLATLNAIDLAPYLGAITAEELGDLIFS
EEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MYLIEPIRNGKRITDGAVALAMQVYVQENLFLDDDILFPYYCDPKVEIGKFQNAVVETNQ
CEEECCCCCCCCCCHHHHHHHHHHHHHCCCEECCCEEEEEECCCCCCHHHHHHHHHHCCH
EYLKEHHIPVVRRDTGGGAVYVDSGAVNICYLINDNGIFGDFKRTYQPAIEALHHLGATE
HHHHHCCCCEEEECCCCCEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCE
VEMSGRNDLVIDGKKVSGAAMTIANGRVYGGYSLLLDVDFEAMEKALKPNRKKIESKGIR
EEECCCCCEEEECCCCCCEEEEEECCEEEECEEEEEECCHHHHHHHHCCCHHHHHHHHHH
SVRSRVGNIREHLAPQYQGITIEEFKDLMVCQLLQIETISQAKRYDLTEKDWQQIDALTE
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
RKYHNWEWNYGNAPQYRYHRDGRFTGGTVDIHLDIKKGYIAACRIYGDFFGKADIAELEG
CCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEEECCCEEEEEEEEHHHCCCCCHHHHCC
HLIGTRMEKEDVLATLNAIDLAPYLGAITAEELGDLIFS
EEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]