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The map label for this gene is cls [C]

Identifier: 71910739

GI number: 71910739

Start: 914572

End: 916149

Strand: Reverse

Name: cls [C]

Synonym: M5005_Spy_0926

Alternate gene names: 71910739

Gene position: 916149-914572 (Counterclockwise)

Preceding gene: 71910740

Following gene: 71910738

Centisome position: 49.83

GC content: 33.71

Gene sequence:

>1578_bases
ATGATTATCAAAAAGAAAGCAAAAGTAAAATATTTGCTCCATAAAGGGAAACATGGATTTTTAAGAGGAATTTTTAGTAG
AACAACAATCATTGTATTATTAATTATTTTGCAATTAGTTTTCTTATTTCAATCTTATGCCTGGATGGAGCAGTACCGTG
TTTGGATAACGATTCTTGAAAGTGTCTTTGCTATTACTATTGTTTTATATTTAGTTAATAGTGATATGGATGCTATTTCA
AGAATGACGTGGTTAATTTTGATTATGATTGCTCCATTACTAGGGTCACTGTTTTTAATTTATACAAAATTGGATTGGGG
TTACAGAGGGCTAAAACAAAGAATAAATCATCTTGTAGATCTGTCTGCACCTTACCTTAGCGATGATGACGCTATATTAG
AGGTATTAAAGGATAGCACATCAACCACATATCATTTAGTACAGTACTTAGAAAGAAGTCGTGGCAACTTTCCGATTTAT
AATAATACAAGGGTAACTTACTTCCCAACAGGTGAAACTTTTTTTGATAGTTTAAAAGAACAGTTATTCTTAGCTAAAAA
GTACATTTTTCTTGAATTTTTTATTATTGCAGAAGGCCAAATGTGGGGAGAAATCCTTAGTATTCTAGAAAAAAAAGTCA
GTGAAGGTGTAGAAGTTAGGGTGTTGTTTGATGGCATGAACGAACTATCAACGCTATCATCAGATTACGCCAAGAGATTA
GAACAAATAGGGATTAAAGCTAAATCATTTTTACCGATTTCACCCTTTATCTCTACCTATTACAATTATCGAGATCACCG
AAAAATTGTCGTTATTGATGGGGAGGTATCATTCACTGGAGGTATTAATCTAGCAGATGAGTACATTAATGAAGTAGAGC
GTTTTGGCCACTGGAAAGATGCTGGTTTAATGCTTGAGGGTGAAGCAACAGACAGCTTTTTAATTTTGTTTTTACAAATG
TGGTCTATCACAGAAAAAGAACTGATTATTGATCCTTATCTTTCAGATCATTCTTTAAAACTTCCTTCAGATGGCTATGT
TATTCCCTACGGTGATTCCCCGCTTGATACTGATAAAATAGGTAAAAATGTTTATATAGACATTTTAAATCATGCTAAAG
AGTACGTTTATATCATGACACCTTACCTTATTTTAGATAGCGAGATGGAACACGCTTTACGGTTTGCATCAGAACGTGGA
GTAGATATCCGAATTATTATGCCAGGAGTGCCAGATAAGGGGGTACCATATGCCTTAGCTAAAACCTATTATAAAGCTTT
GATGTCTTCTGGAGTAAAAATTTATGAGTATCAACCAGGGTTCGTTCATTCAAAGGTATTTATCTCTGATAATACAAAAG
CAGTTGTCGGAACAATTAACTTAGACTATCGTAGCCTTTATCATCATTTTGAATGCGCGACTTATTTATATCGTGTGTCA
GTTATTGCTGATATTGTGAATGACTTTAATGAAGCTCAAAAGCAATCACTATTGATGACATCAGATCATTTGACGCAACG
TCCTTGGTATCAAAAATTGATAGGATTATTAGTACGAACAATTGCCCCTCTCTTGTAA

Upstream 100 bases:

>100_bases
CCTCGGTCTCTTTTAGGAAAAATTGCTCGAATAATTCGAGCAAGCGCTCCTTTCTTCCCGCTTTACTGTGATATAATAAG
GTAAATGAGATTTTGGAGGG

Downstream 100 bases:

>100_bases
TTTTCGTAAAATATGTTATAATATCTGTATGCGATGAGTCGATTGTGACTGCAAAGTCACTTTTGCGCAGGGGAGGTCAT
TAAGGCGGGAAGCCGACTTT

Product: cardiolipin synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MIIKKKAKVKYLLHKGKHGFLRGIFSRTTIIVLLIILQLVFLFQSYAWMEQYRVWITILESVFAITIVLYLVNSDMDAIS
RMTWLILIMIAPLLGSLFLIYTKLDWGYRGLKQRINHLVDLSAPYLSDDDAILEVLKDSTSTTYHLVQYLERSRGNFPIY
NNTRVTYFPTGETFFDSLKEQLFLAKKYIFLEFFIIAEGQMWGEILSILEKKVSEGVEVRVLFDGMNELSTLSSDYAKRL
EQIGIKAKSFLPISPFISTYYNYRDHRKIVVIDGEVSFTGGINLADEYINEVERFGHWKDAGLMLEGEATDSFLILFLQM
WSITEKELIIDPYLSDHSLKLPSDGYVIPYGDSPLDTDKIGKNVYIDILNHAKEYVYIMTPYLILDSEMEHALRFASERG
VDIRIIMPGVPDKGVPYALAKTYYKALMSSGVKIYEYQPGFVHSKVFISDNTKAVVGTINLDYRSLYHHFECATYLYRVS
VIADIVNDFNEAQKQSLLMTSDHLTQRPWYQKLIGLLVRTIAPLL

Sequences:

>Translated_525_residues
MIIKKKAKVKYLLHKGKHGFLRGIFSRTTIIVLLIILQLVFLFQSYAWMEQYRVWITILESVFAITIVLYLVNSDMDAIS
RMTWLILIMIAPLLGSLFLIYTKLDWGYRGLKQRINHLVDLSAPYLSDDDAILEVLKDSTSTTYHLVQYLERSRGNFPIY
NNTRVTYFPTGETFFDSLKEQLFLAKKYIFLEFFIIAEGQMWGEILSILEKKVSEGVEVRVLFDGMNELSTLSSDYAKRL
EQIGIKAKSFLPISPFISTYYNYRDHRKIVVIDGEVSFTGGINLADEYINEVERFGHWKDAGLMLEGEATDSFLILFLQM
WSITEKELIIDPYLSDHSLKLPSDGYVIPYGDSPLDTDKIGKNVYIDILNHAKEYVYIMTPYLILDSEMEHALRFASERG
VDIRIIMPGVPDKGVPYALAKTYYKALMSSGVKIYEYQPGFVHSKVFISDNTKAVVGTINLDYRSLYHHFECATYLYRVS
VIADIVNDFNEAQKQSLLMTSDHLTQRPWYQKLIGLLVRTIAPLL
>Mature_525_residues
MIIKKKAKVKYLLHKGKHGFLRGIFSRTTIIVLLIILQLVFLFQSYAWMEQYRVWITILESVFAITIVLYLVNSDMDAIS
RMTWLILIMIAPLLGSLFLIYTKLDWGYRGLKQRINHLVDLSAPYLSDDDAILEVLKDSTSTTYHLVQYLERSRGNFPIY
NNTRVTYFPTGETFFDSLKEQLFLAKKYIFLEFFIIAEGQMWGEILSILEKKVSEGVEVRVLFDGMNELSTLSSDYAKRL
EQIGIKAKSFLPISPFISTYYNYRDHRKIVVIDGEVSFTGGINLADEYINEVERFGHWKDAGLMLEGEATDSFLILFLQM
WSITEKELIIDPYLSDHSLKLPSDGYVIPYGDSPLDTDKIGKNVYIDILNHAKEYVYIMTPYLILDSEMEHALRFASERG
VDIRIIMPGVPDKGVPYALAKTYYKALMSSGVKIYEYQPGFVHSKVFISDNTKAVVGTINLDYRSLYHHFECATYLYRVS
VIADIVNDFNEAQKQSLLMTSDHLTQRPWYQKLIGLLVRTIAPLL

Specific function: Catalyzes The Reversible Phosphatidyl Group Transfer From One Phosphatidylglycerol Molecule To Another To Form Cardiolipin (Cl) (Diphosphatidylglycerol) And Glycerol. Affects Resistance To The Gyrase Inhibitor Novobiocin. [C]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=488, Percent_Identity=24.7950819672131, Blast_Score=161, Evalue=7e-41,
Organism=Escherichia coli, GI1787007, Length=357, Percent_Identity=26.6106442577031, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 60577; Mature: 60577

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIKKKAKVKYLLHKGKHGFLRGIFSRTTIIVLLIILQLVFLFQSYAWMEQYRVWITILE
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVFAITIVLYLVNSDMDAISRMTWLILIMIAPLLGSLFLIYTKLDWGYRGLKQRINHLVD
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LSAPYLSDDDAILEVLKDSTSTTYHLVQYLERSRGNFPIYNNTRVTYFPTGETFFDSLKE
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEECCCEEEEEECCHHHHHHHHH
QLFLAKKYIFLEFFIIAEGQMWGEILSILEKKVSEGVEVRVLFDGMNELSTLSSDYAKRL
HHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHH
EQIGIKAKSFLPISPFISTYYNYRDHRKIVVIDGEVSFTGGINLADEYINEVERFGHWKD
HHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCEEECCCCCHHHHHHHHHHHCCCCCC
AGLMLEGEATDSFLILFLQMWSITEKELIIDPYLSDHSLKLPSDGYVIPYGDSPLDTDKI
CCEEEECCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCEECCCCCEEEECCCCCCCHHHC
GKNVYIDILNHAKEYVYIMTPYLILDSEMEHALRFASERGVDIRIIMPGVPDKGVPYALA
CCCEEEHHHHCCCCEEEEECCCEEECHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
KTYYKALMSSGVKIYEYQPGFVHSKVFISDNTKAVVGTINLDYRSLYHHFECATYLYRVS
HHHHHHHHHCCCEEEEECCCCEEEEEEEECCCEEEEEEEECHHHHHHHHHHHHHHHHHHH
VIADIVNDFNEAQKQSLLMTSDHLTQRPWYQKLIGLLVRTIAPLL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIIKKKAKVKYLLHKGKHGFLRGIFSRTTIIVLLIILQLVFLFQSYAWMEQYRVWITILE
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVFAITIVLYLVNSDMDAISRMTWLILIMIAPLLGSLFLIYTKLDWGYRGLKQRINHLVD
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LSAPYLSDDDAILEVLKDSTSTTYHLVQYLERSRGNFPIYNNTRVTYFPTGETFFDSLKE
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEECCCEEEEEECCHHHHHHHHH
QLFLAKKYIFLEFFIIAEGQMWGEILSILEKKVSEGVEVRVLFDGMNELSTLSSDYAKRL
HHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHH
EQIGIKAKSFLPISPFISTYYNYRDHRKIVVIDGEVSFTGGINLADEYINEVERFGHWKD
HHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCEEECCCCCHHHHHHHHHHHCCCCCC
AGLMLEGEATDSFLILFLQMWSITEKELIIDPYLSDHSLKLPSDGYVIPYGDSPLDTDKI
CCEEEECCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCEECCCCCEEEECCCCCCCHHHC
GKNVYIDILNHAKEYVYIMTPYLILDSEMEHALRFASERGVDIRIIMPGVPDKGVPYALA
CCCEEEHHHHCCCCEEEEECCCEEECHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
KTYYKALMSSGVKIYEYQPGFVHSKVFISDNTKAVVGTINLDYRSLYHHFECATYLYRVS
HHHHHHHHHCCCEEEEECCCCEEEEEEEECCCEEEEEEEECHHHHHHHHHHHHHHHHHHH
VIADIVNDFNEAQKQSLLMTSDHLTQRPWYQKLIGLLVRTIAPLL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12397186; 2761387 [H]