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The map label for this gene is apbE [H]

Identifier: 71910673

GI number: 71910673

Start: 851253

End: 852191

Strand: Reverse

Name: apbE [H]

Synonym: M5005_Spy_0860

Alternate gene names: 71910673

Gene position: 852191-851253 (Counterclockwise)

Preceding gene: 71910674

Following gene: 71910667

Centisome position: 46.35

GC content: 37.06

Gene sequence:

>939_bases
ATGTTTGTTACGCAACAATTAAAGCTAATGGGAACAGTTATTGATATTCAGATCGAATCTGACAAAGCTTGCCAACAACT
AAGTAGAGTCATTGACTTATTATATACTTACAAAAATCGTTTTTCAGCTAATGATTCAAACTCTGAATTGATGGCTATTA
ATCAGGCGGCTGGTGTTAAACCCGTCAGCGTTCACTCTGATTTATTTAACCTAATCCAAATTGGTAAAGCTCATAGCTTG
TCAACACCAAGTAATCTTAATATCGCCATTGGGCCACTTGTTCAAGCCTGGCGAATTGGATTTGAAGATGCTAGAGTGCC
TAGTCATAACCTTATCTCACAACAGTTAGCTCTAACAGATCCTAGACAGGTTCTTATAGATGACAAAAAACAGACTGTCT
TTTTACAACAAGTAGGAATGGCTTTAGACTTAGGAGCCTTAGCAAAAGGTTATATTACTGATAAGATTATGGCCTACCTG
ATTGAAGATGGTATTGACTCTGCCCTTATTAATTTAGGTGGAAATGTACGCGTTCATGGACCTAATCCGAAAAGCCCTGA
CAAGACTTTTCGAATTGGCATTCAAAAACCAGATGCTAAACGTGGGCAACATCTTGGTGTCATCAAGGTTAACAATCACT
CTGTTGTGACTTCTGGAATTTATGAAAGGCAGTTTACATCCAAAGGGAAACAGTATCATCATATCTTAGATCGTCAGACA
GGCTACCCTATAGAAACAGATATGCTTAGCTTAACGATCATGGCTCCTTCTTCTTTTTATTGTGACATATGGACTACCCG
TTTATTTGGTCTTGATAGCTCAATGATTATCACACTTTTAAATACCTTTGATAATATTGAAGGTCTTCTAGTAACAAGAA
AGCACCATGTCCTTATGTCAAATGGTTTGAGACACTATTTTCAACCATACTATCATTAA

Upstream 100 bases:

>100_bases
CCTGAAGGAACTTACTATCCTCAAGGTGAAATGAAACAAAAATCATAATGACCTTCCCCCACTCCTTAGTGGGGGTATTC
TGCTAAAAAGAGGACTAATT

Downstream 100 bases:

>100_bases
AAGGGGTTAACGGTTTGCTCAAAACTGATCACAGAAAAAACGGCTCAATTAGCCGTTTTTTAATTATTCATCTTGTTTGT
CTTTGCCATTTAAAACTAAA

Product: thiamine biosynthesis lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MFVTQQLKLMGTVIDIQIESDKACQQLSRVIDLLYTYKNRFSANDSNSELMAINQAAGVKPVSVHSDLFNLIQIGKAHSL
STPSNLNIAIGPLVQAWRIGFEDARVPSHNLISQQLALTDPRQVLIDDKKQTVFLQQVGMALDLGALAKGYITDKIMAYL
IEDGIDSALINLGGNVRVHGPNPKSPDKTFRIGIQKPDAKRGQHLGVIKVNNHSVVTSGIYERQFTSKGKQYHHILDRQT
GYPIETDMLSLTIMAPSSFYCDIWTTRLFGLDSSMIITLLNTFDNIEGLLVTRKHHVLMSNGLRHYFQPYYH

Sequences:

>Translated_312_residues
MFVTQQLKLMGTVIDIQIESDKACQQLSRVIDLLYTYKNRFSANDSNSELMAINQAAGVKPVSVHSDLFNLIQIGKAHSL
STPSNLNIAIGPLVQAWRIGFEDARVPSHNLISQQLALTDPRQVLIDDKKQTVFLQQVGMALDLGALAKGYITDKIMAYL
IEDGIDSALINLGGNVRVHGPNPKSPDKTFRIGIQKPDAKRGQHLGVIKVNNHSVVTSGIYERQFTSKGKQYHHILDRQT
GYPIETDMLSLTIMAPSSFYCDIWTTRLFGLDSSMIITLLNTFDNIEGLLVTRKHHVLMSNGLRHYFQPYYH
>Mature_312_residues
MFVTQQLKLMGTVIDIQIESDKACQQLSRVIDLLYTYKNRFSANDSNSELMAINQAAGVKPVSVHSDLFNLIQIGKAHSL
STPSNLNIAIGPLVQAWRIGFEDARVPSHNLISQQLALTDPRQVLIDDKKQTVFLQQVGMALDLGALAKGYITDKIMAYL
IEDGIDSALINLGGNVRVHGPNPKSPDKTFRIGIQKPDAKRGQHLGVIKVNNHSVVTSGIYERQFTSKGKQYHHILDRQT
GYPIETDMLSLTIMAPSSFYCDIWTTRLFGLDSSMIITLLNTFDNIEGLLVTRKHHVLMSNGLRHYFQPYYH

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=255, Percent_Identity=29.0196078431373, Blast_Score=116, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 34935; Mature: 34935

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVTQQLKLMGTVIDIQIESDKACQQLSRVIDLLYTYKNRFSANDSNSELMAINQAAGVK
CCCHHHHHEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCC
PVSVHSDLFNLIQIGKAHSLSTPSNLNIAIGPLVQAWRIGFEDARVPSHNLISQQLALTD
EEEHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHCCC
PRQVLIDDKKQTVFLQQVGMALDLGALAKGYITDKIMAYLIEDGIDSALINLGGNVRVHG
CHHEEECCCHHEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEC
PNPKSPDKTFRIGIQKPDAKRGQHLGVIKVNNHSVVTSGIYERQFTSKGKQYHHILDRQT
CCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCEEEECCHHHHHHHHCCHHHHHHHHCCC
GYPIETDMLSLTIMAPSSFYCDIWTTRLFGLDSSMIITLLNTFDNIEGLLVTRKHHVLMS
CCCCCCCEEEEEEECCCCEEEHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECHHHHHH
NGLRHYFQPYYH
CCHHHHCCCCCC
>Mature Secondary Structure
MFVTQQLKLMGTVIDIQIESDKACQQLSRVIDLLYTYKNRFSANDSNSELMAINQAAGVK
CCCHHHHHEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCC
PVSVHSDLFNLIQIGKAHSLSTPSNLNIAIGPLVQAWRIGFEDARVPSHNLISQQLALTD
EEEHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHCCC
PRQVLIDDKKQTVFLQQVGMALDLGALAKGYITDKIMAYLIEDGIDSALINLGGNVRVHG
CHHEEECCCHHEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEC
PNPKSPDKTFRIGIQKPDAKRGQHLGVIKVNNHSVVTSGIYERQFTSKGKQYHHILDRQT
CCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCEEEECCHHHHHHHHCCHHHHHHHHCCC
GYPIETDMLSLTIMAPSSFYCDIWTTRLFGLDSSMIITLLNTFDNIEGLLVTRKHHVLMS
CCCCCCCEEEEEEECCCCEEEHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECHHHHHH
NGLRHYFQPYYH
CCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]