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The map label for this gene is srtK [H]

Identifier: 71910618

GI number: 71910618

Start: 805601

End: 806947

Strand: Direct

Name: srtK [H]

Synonym: M5005_Spy_0805

Alternate gene names: 71910618

Gene position: 805601-806947 (Clockwise)

Preceding gene: 71910617

Following gene: 71910619

Centisome position: 43.82

GC content: 29.03

Gene sequence:

>1347_bases
ATGTTTAAAAAATATTCAATAAAAAATCGTGTTTGGCGCGCCGTCGTTGAAATTGTCTTTGGAGTTTGTATCACTGTTTT
AATGATTGCGATAATATCGCTTTCTTTTTCAAAGTTAAATATAGTGACATCTCATAATGTGGGAGAAGAATTTTATATTA
AAGATAAACAGTCAATCAAACAGTTGAACAATTATATGAAGACATTGGGATTAGATTATGTTGTTTTTGATAGAAAAACA
GATAAAGCTATGGAAGGAAAATATTTATCTAAAGAATTTTCTTTATTTAACGAAGTTGCAGAAGAAAAAAATAATCTGAC
TTTTAATTCTGTACATTATGATTTATATACTAATATCAATTATAATATTGTTATAAGATATAATGAGATACCGGAGTTCT
CTAACCATTACCTTAGAAATGTTTCATATAACATGTTGACATTTTATATTCTGGGAATAGGAACGAGTATCAGCATTGTA
GTTGCTTTAACACGGTTTGTAAAAGAAATTTCTTTGAATTTCAAGGAAATCAAGAAGTTAGCAAATAAAATGGGGATAGA
AGTCCTTTCTGAAAATGAAAATTATTCTAAAATAATCGAGTTTGATGATATACTTAGAACTTTACATATTAAAGGTGATA
ATTTGAAAAGTTTGATTGAGCGAGAAATATTGGAAAAGCAAGATCTATCCTTTCAAATTGCAGCACTTTCGCATGATATA
AAAACACCTTTAACGGTTTTAAAAGGAAATCTTGAGTTATTAGAATTGACTACTTTAAATAAAAATCAGGAAGGTTATAT
TGTATCTATGAACAATAGTATTTCTGTTTTTGAAGGCTATTTCAATTCTTTAATATCTTATACACGCATGCTTTCAGAGG
ATAGATCAGTGAAACTAATTTTAGTTGAGGAGCTATTAAGTGAGTTACATTTTGAAGTCGATGACTTATTGAATATCAAT
AATATTGAATTTTCTATTTGTAATAGACTTATTATCACTTCTTTTTATGGAGATGAGGAAAATCTGATAAGAGCTCTTTC
TAACCTTTTAGTTAATGCAATAAGATTTATGCCCGTATTAGATAAAAAAATCGAAGTTATACTGTCTGAGTCAGGAGAGC
AAATTCATTTTGAAATATGGAATAATGGTGAGCGTTTCAGTGATAGTACACTGAAAAAGGGAGATAAATTATTTTACACG
GAAGATTATAGCAGAGGTAACAAACATTATGGAATCGGTTTGGCATTTGTTAAAGGCGTTGCTATCAAACACGGTGGGAA
TCTTCAATTGAATAATCCAGCTAGAGGAGGTGCTAGCGCCATAATCTCGATAAAAAAGAAAATTTAA

Upstream 100 bases:

>100_bases
TTTCATTCTATTTCAGAATATATCTATCAGATTAGAATGAAATTTTCAAGTTTTGGAATTAATCCAATAAAAACTATTCG
CGGGATTGGTTATAAATGGG

Downstream 100 bases:

>100_bases
TAGATTTGATTGAAACTGGAGTATTTCAGGCAAAAAATCGTAAATTTTTGGCTAAAATACTTTTTTTCTGAACAAATTCT
GAACTTGGGGTGTCATAATA

Product: nisin biosynthesis sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MFKKYSIKNRVWRAVVEIVFGVCITVLMIAIISLSFSKLNIVTSHNVGEEFYIKDKQSIKQLNNYMKTLGLDYVVFDRKT
DKAMEGKYLSKEFSLFNEVAEEKNNLTFNSVHYDLYTNINYNIVIRYNEIPEFSNHYLRNVSYNMLTFYILGIGTSISIV
VALTRFVKEISLNFKEIKKLANKMGIEVLSENENYSKIIEFDDILRTLHIKGDNLKSLIEREILEKQDLSFQIAALSHDI
KTPLTVLKGNLELLELTTLNKNQEGYIVSMNNSISVFEGYFNSLISYTRMLSEDRSVKLILVEELLSELHFEVDDLLNIN
NIEFSICNRLIITSFYGDEENLIRALSNLLVNAIRFMPVLDKKIEVILSESGEQIHFEIWNNGERFSDSTLKKGDKLFYT
EDYSRGNKHYGIGLAFVKGVAIKHGGNLQLNNPARGGASAIISIKKKI

Sequences:

>Translated_448_residues
MFKKYSIKNRVWRAVVEIVFGVCITVLMIAIISLSFSKLNIVTSHNVGEEFYIKDKQSIKQLNNYMKTLGLDYVVFDRKT
DKAMEGKYLSKEFSLFNEVAEEKNNLTFNSVHYDLYTNINYNIVIRYNEIPEFSNHYLRNVSYNMLTFYILGIGTSISIV
VALTRFVKEISLNFKEIKKLANKMGIEVLSENENYSKIIEFDDILRTLHIKGDNLKSLIEREILEKQDLSFQIAALSHDI
KTPLTVLKGNLELLELTTLNKNQEGYIVSMNNSISVFEGYFNSLISYTRMLSEDRSVKLILVEELLSELHFEVDDLLNIN
NIEFSICNRLIITSFYGDEENLIRALSNLLVNAIRFMPVLDKKIEVILSESGEQIHFEIWNNGERFSDSTLKKGDKLFYT
EDYSRGNKHYGIGLAFVKGVAIKHGGNLQLNNPARGGASAIISIKKKI
>Mature_448_residues
MFKKYSIKNRVWRAVVEIVFGVCITVLMIAIISLSFSKLNIVTSHNVGEEFYIKDKQSIKQLNNYMKTLGLDYVVFDRKT
DKAMEGKYLSKEFSLFNEVAEEKNNLTFNSVHYDLYTNINYNIVIRYNEIPEFSNHYLRNVSYNMLTFYILGIGTSISIV
VALTRFVKEISLNFKEIKKLANKMGIEVLSENENYSKIIEFDDILRTLHIKGDNLKSLIEREILEKQDLSFQIAALSHDI
KTPLTVLKGNLELLELTTLNKNQEGYIVSMNNSISVFEGYFNSLISYTRMLSEDRSVKLILVEELLSELHFEVDDLLNIN
NIEFSICNRLIITSFYGDEENLIRALSNLLVNAIRFMPVLDKKIEVILSESGEQIHFEIWNNGERFSDSTLKKGDKLFYT
EDYSRGNKHYGIGLAFVKGVAIKHGGNLQLNNPARGGASAIISIKKKI

Specific function: Member of the two-component regulatory system nisK/nisR involved in the regulation of the biosynthesis of lantibiotic nisin. NisK may function as a membrane-associated protein kinase that phosphorylates nisR in response to environmental signals [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786912, Length=227, Percent_Identity=27.7533039647577, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR008358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51472; Mature: 51472

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKKYSIKNRVWRAVVEIVFGVCITVLMIAIISLSFSKLNIVTSHNVGEEFYIKDKQSIK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCCCEEEECCHHHHH
QLNNYMKTLGLDYVVFDRKTDKAMEGKYLSKEFSLFNEVAEEKNNLTFNSVHYDLYTNIN
HHHHHHHHHCCCEEEEECCCCCHHCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCC
YNIVIRYNEIPEFSNHYLRNVSYNMLTFYILGIGTSISIVVALTRFVKEISLNFKEIKKL
EEEEEEECCCCCHHHHHHHCCCHHHHHHEEHHCCCHHHHHHHHHHHHHHHCCCHHHHHHH
ANKMGIEVLSENENYSKIIEFDDILRTLHIKGDNLKSLIEREILEKQDLSFQIAALSHDI
HHHCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEHHCCC
KTPLTVLKGNLELLELTTLNKNQEGYIVSMNNSISVFEGYFNSLISYTRMLSEDRSVKLI
CCCHHHHCCCCEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
LVEELLSELHFEVDDLLNINNIEFSICNRLIITSFYGDEENLIRALSNLLVNAIRFMPVL
EHHHHHHHHCCCHHHHCCCCCCHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
DKKIEVILSESGEQIHFEIWNNGERFSDSTLKKGDKLFYTEDYSRGNKHYGIGLAFVKGV
HHHHHHHEECCCCEEEEEEECCCCCCCCHHHCCCCEEEEECCCCCCCCCEEEHHHHHHCE
AIKHGGNLQLNNPARGGASAIISIKKKI
EEECCCCEEECCCCCCCCCEEEEEECCC
>Mature Secondary Structure
MFKKYSIKNRVWRAVVEIVFGVCITVLMIAIISLSFSKLNIVTSHNVGEEFYIKDKQSIK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCCCEEEECCHHHHH
QLNNYMKTLGLDYVVFDRKTDKAMEGKYLSKEFSLFNEVAEEKNNLTFNSVHYDLYTNIN
HHHHHHHHHCCCEEEEECCCCCHHCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCC
YNIVIRYNEIPEFSNHYLRNVSYNMLTFYILGIGTSISIVVALTRFVKEISLNFKEIKKL
EEEEEEECCCCCHHHHHHHCCCHHHHHHEEHHCCCHHHHHHHHHHHHHHHCCCHHHHHHH
ANKMGIEVLSENENYSKIIEFDDILRTLHIKGDNLKSLIEREILEKQDLSFQIAALSHDI
HHHCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEHHCCC
KTPLTVLKGNLELLELTTLNKNQEGYIVSMNNSISVFEGYFNSLISYTRMLSEDRSVKLI
CCCHHHHCCCCEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
LVEELLSELHFEVDDLLNINNIEFSICNRLIITSFYGDEENLIRALSNLLVNAIRFMPVL
EHHHHHHHHCCCHHHHCCCCCCHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
DKKIEVILSESGEQIHFEIWNNGERFSDSTLKKGDKLFYTEDYSRGNKHYGIGLAFVKGV
HHHHHHHEECCCCEEEEEEECCCCCCCCHHHCCCCEEEEECCCCCCCCCEEEHHHHHHCE
AIKHGGNLQLNNPARGGASAIISIKKKI
EEECCCCEEECCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8161176 [H]