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The map label for this gene is nrdE [H]

Identifier: 71893571

GI number: 71893571

Start: 251268

End: 253409

Strand: Reverse

Name: nrdE [H]

Synonym: MHJ_0215

Alternate gene names: 71893571

Gene position: 253409-251268 (Counterclockwise)

Preceding gene: 71893572

Following gene: 71893570

Centisome position: 28.24

GC content: 31.19

Gene sequence:

>2142_bases
CTGAATTTTGATAAAAATCAGGAAAAAAGTTATCTTAGCTTAAATGCAAATAGCAAAATTTTTGCAAATCATCCTGATTC
ACATAAATTTGATTTATTAGCAGCAGAGAAATATATTAATGAAGAAATTAATCCAAAATTTAAATTTTTCAATTCTTTTA
AGGAAAGAATTGAATTTTTAGTTCAAAATAATTATTATGATCCGAAAGTTTTAGGACAATATTCATTCCAAGAACTTGAG
AAATTAAATACAAAAGCTTGAAGTTATAATCATTTTTTTTCTTCTTTTATGGGGGCTTTTAAATTTTATTCAACTTATGG
ACTAAAATCTAATGATAATTTAGTATATTTTGAACATTTTCCCGATCGAGTTTTACTAAATAGTTTATTTTTAGGAAAAG
GAGATTTTCAAAAAGCTGAAAAAATTCTTGAACAAATTATGTTAGGTCGATTTCAACCGGCAACCCCGACATTTTTAAAT
GCCGGAAAATTAAACAGAGGTGAATTTGTCTCTTGTTATTTAATCCGAGTTGAAGATAATATGGAATCAATTTCCAGGGC
AATTACTACCTCACTCCAACTTTCAAAACGTGGCGGTGGTGTTAGTGTTTTACTTTCAAATTTGCGCGAACAAGGTGCCC
CGATTAAAAACATTGAAAATCAAGCAACTGGTGTAATTCCGGTAATGAAAATTCTTGAAGATTCATTTTCTTATGCAAAC
CAGTTAGGACAACGTCAAGGTGCTGGGGCTGTTTATTTAAATGTCCACCATCCGGATATTCTGGCTTTTTTAGATACCAA
AAGGGAAAATGCTGATGAAAAAATCAGGATAAAATCGCTTTCTCTTGGGATAATAATTCCGAATATTACTTTTGAATTAG
CAAAAAATAATGAAAAAATGGCGCTTTTTTCTGTCTATGATGTTGAAAAAGTATATCAAAAAGCTTTTAGTGATATTTCT
ATTAGTGAAAAATATTATCAAATGCTAGCAAATCCAGAAATTAAAAAAACATTTATTTCGGCAAGAAAGTTATTTAAAAT
TATTGCCGAGCTACATTTTGAAAGTGGTTATCCTTATATTTTATTTGAAGATATTGTCAATAAAGAAAATGCTCATCCTG
ATCGCGTTGTGATGTCAAATTTATGTAGCGAAATCACACAGCCTTCAACTCCAAGCACATTTTTACCCGATCTAAGTTTT
AAAGAAATAGGCAAGGATATTTGTTGTAATTTAGGATCGCTAAATATTGATAAGGCGATGGAATCAGGCCCTGATTTTGA
AGAAATGGTTTATTATTCAGTGTTAGCTCTTGATGTTGTCTCGCGAAATTCTGATCTCTCAGTAGCGCCTTCAATCCAAA
AAGGAAATAATTTAAATCATGCAATTGGCCTTGGGGCGATGAATTTACACGGGTTTTTGGCTAAAAATGAAATTTTTTAT
GACTCAGAGGAAGCGCTTGATTTTACTAACATTTTTTTCTATACTTTAGCTTTTTCAGCTTTTAAAGCCTCTGAAAAACT
TGCAAAAAAATATGGTCCTTTTTCTGGATTTGAAAAAACCAAATTTGCATCAGGAGAATATTTTGATAAATATACAAAAG
TTTCCCCGAATACTTTTGTACCAAAAACACATAAAATTAAAGAACTTTTTGCAAAATATCAAGTTGAAATTCCGACTCAA
AAAGATTGAATTCATCTTGTTGAAAAAATTAAAAAAACAGGAATTGCCAACTCACATCTAATGGCGATTGCTCCTACAGG
CTCAATTTCCTATCTTAGTTCCTGCACGCCTTCGCTTCAGCCCGTTGTTGCCCCAGTTGAGATAAGAAAAGAAGGAAAAT
TAGGGAGAATTTATGTTCCTTCATATAAATTAAGTCCTAAAACATACAAATTTTATCAAAACGGAGCTTATGAATTAGGT
CCAATTCCGATTATAAACATTGTCTCAGAAGCACAAAAACATGTTGATCAAGCAATCTCGATGACCTTATTTATGACCGA
CAAGGCGACTACTCGAGACTTAAATCGAGCTTATATTTATGCTTTTAAAAAAAATTGTAAGTCAATTTACTATGTAAGAA
TTCGTCAAGATGTCCTTGAGGATTCAGAAAATTATGAAACTTGTCAATCTTGCCAGATTTAA

Upstream 100 bases:

>100_bases
TTAGAAGATTTTTGAGGAAAATAAGCTAAAAAATAATGAATATAATAGTTAGTTCGCTAACTAAATTGGGAGAAAATAAA
TGAAAAAAGTGGGTTTGGAT

Downstream 100 bases:

>100_bases
AACAAATTAAAAAATACTTAAATTTAAAGTATTTTTTACTAAATAAAGGTTAAAAGTACAACTAATTTATATAAAGTACT
AAAGATAAATTCCTTGTTTT

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase [H]

Number of amino acids: Translated: 713; Mature: 713

Protein sequence:

>713_residues
MNFDKNQEKSYLSLNANSKIFANHPDSHKFDLLAAEKYINEEINPKFKFFNSFKERIEFLVQNNYYDPKVLGQYSFQELE
KLNTKAWSYNHFFSSFMGAFKFYSTYGLKSNDNLVYFEHFPDRVLLNSLFLGKGDFQKAEKILEQIMLGRFQPATPTFLN
AGKLNRGEFVSCYLIRVEDNMESISRAITTSLQLSKRGGGVSVLLSNLREQGAPIKNIENQATGVIPVMKILEDSFSYAN
QLGQRQGAGAVYLNVHHPDILAFLDTKRENADEKIRIKSLSLGIIIPNITFELAKNNEKMALFSVYDVEKVYQKAFSDIS
ISEKYYQMLANPEIKKTFISARKLFKIIAELHFESGYPYILFEDIVNKENAHPDRVVMSNLCSEITQPSTPSTFLPDLSF
KEIGKDICCNLGSLNIDKAMESGPDFEEMVYYSVLALDVVSRNSDLSVAPSIQKGNNLNHAIGLGAMNLHGFLAKNEIFY
DSEEALDFTNIFFYTLAFSAFKASEKLAKKYGPFSGFEKTKFASGEYFDKYTKVSPNTFVPKTHKIKELFAKYQVEIPTQ
KDWIHLVEKIKKTGIANSHLMAIAPTGSISYLSSCTPSLQPVVAPVEIRKEGKLGRIYVPSYKLSPKTYKFYQNGAYELG
PIPIINIVSEAQKHVDQAISMTLFMTDKATTRDLNRAYIYAFKKNCKSIYYVRIRQDVLEDSENYETCQSCQI

Sequences:

>Translated_713_residues
MNFDKNQEKSYLSLNANSKIFANHPDSHKFDLLAAEKYINEEINPKFKFFNSFKERIEFLVQNNYYDPKVLGQYSFQELE
KLNTKA*SYNHFFSSFMGAFKFYSTYGLKSNDNLVYFEHFPDRVLLNSLFLGKGDFQKAEKILEQIMLGRFQPATPTFLN
AGKLNRGEFVSCYLIRVEDNMESISRAITTSLQLSKRGGGVSVLLSNLREQGAPIKNIENQATGVIPVMKILEDSFSYAN
QLGQRQGAGAVYLNVHHPDILAFLDTKRENADEKIRIKSLSLGIIIPNITFELAKNNEKMALFSVYDVEKVYQKAFSDIS
ISEKYYQMLANPEIKKTFISARKLFKIIAELHFESGYPYILFEDIVNKENAHPDRVVMSNLCSEITQPSTPSTFLPDLSF
KEIGKDICCNLGSLNIDKAMESGPDFEEMVYYSVLALDVVSRNSDLSVAPSIQKGNNLNHAIGLGAMNLHGFLAKNEIFY
DSEEALDFTNIFFYTLAFSAFKASEKLAKKYGPFSGFEKTKFASGEYFDKYTKVSPNTFVPKTHKIKELFAKYQVEIPTQ
KD*IHLVEKIKKTGIANSHLMAIAPTGSISYLSSCTPSLQPVVAPVEIRKEGKLGRIYVPSYKLSPKTYKFYQNGAYELG
PIPIINIVSEAQKHVDQAISMTLFMTDKATTRDLNRAYIYAFKKNCKSIYYVRIRQDVLEDSENYETCQSCQI
>Mature_713_residues
MNFDKNQEKSYLSLNANSKIFANHPDSHKFDLLAAEKYINEEINPKFKFFNSFKERIEFLVQNNYYDPKVLGQYSFQELE
KLNTKA*SYNHFFSSFMGAFKFYSTYGLKSNDNLVYFEHFPDRVLLNSLFLGKGDFQKAEKILEQIMLGRFQPATPTFLN
AGKLNRGEFVSCYLIRVEDNMESISRAITTSLQLSKRGGGVSVLLSNLREQGAPIKNIENQATGVIPVMKILEDSFSYAN
QLGQRQGAGAVYLNVHHPDILAFLDTKRENADEKIRIKSLSLGIIIPNITFELAKNNEKMALFSVYDVEKVYQKAFSDIS
ISEKYYQMLANPEIKKTFISARKLFKIIAELHFESGYPYILFEDIVNKENAHPDRVVMSNLCSEITQPSTPSTFLPDLSF
KEIGKDICCNLGSLNIDKAMESGPDFEEMVYYSVLALDVVSRNSDLSVAPSIQKGNNLNHAIGLGAMNLHGFLAKNEIFY
DSEEALDFTNIFFYTLAFSAFKASEKLAKKYGPFSGFEKTKFASGEYFDKYTKVSPNTFVPKTHKIKELFAKYQVEIPTQ
KD*IHLVEKIKKTGIANSHLMAIAPTGSISYLSSCTPSLQPVVAPVEIRKEGKLGRIYVPSYKLSPKTYKFYQNGAYELG
PIPIINIVSEAQKHVDQAISMTLFMTDKATTRDLNRAYIYAFKKNCKSIYYVRIRQDVLEDSENYETCQSCQI

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=657, Percent_Identity=24.0487062404871, Blast_Score=162, Evalue=1e-39,
Organism=Escherichia coli, GI1789030, Length=684, Percent_Identity=52.6315789473684, Blast_Score=732, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=584, Percent_Identity=26.541095890411, Blast_Score=164, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17555242, Length=663, Percent_Identity=24.8868778280543, Blast_Score=162, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6320914, Length=668, Percent_Identity=25.2994011976048, Blast_Score=165, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6322123, Length=667, Percent_Identity=24.7376311844078, Blast_Score=157, Evalue=4e-39,
Organism=Drosophila melanogaster, GI17352481, Length=618, Percent_Identity=23.7864077669903, Blast_Score=152, Evalue=7e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 80789; Mature: 80789

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFDKNQEKSYLSLNANSKIFANHPDSHKFDLLAAEKYINEEINPKFKFFNSFKERIEFL
CCCCCCCCCCEEEECCCCEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
VQNNYYDPKVLGQYSFQELEKLNTKASYNHFFSSFMGAFKFYSTYGLKSNDNLVYFEHFP
HHCCCCCCHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCC
DRVLLNSLFLGKGDFQKAEKILEQIMLGRFQPATPTFLNAGKLNRGEFVSCYLIRVEDNM
CHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCEEEEEEEEEECCH
ESISRAITTSLQLSKRGGGVSVLLSNLREQGAPIKNIENQATGVIPVMKILEDSFSYANQ
HHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LGQRQGAGAVYLNVHHPDILAFLDTKRENADEKIRIKSLSLGIIIPNITFELAKNNEKMA
HHHCCCCCEEEEEECCCCEEEEEECCCCCCCCEEEEEEEEEEEEECCCEEEEECCCCCEE
LFSVYDVEKVYQKAFSDISISEKYYQMLANPEIKKTFISARKLFKIIAELHFESGYPYIL
EEEHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
FEDIVNKENAHPDRVVMSNLCSEITQPSTPSTFLPDLSFKEIGKDICCNLGSLNIDKAME
EHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCHHHHCCCCCCCHHHHHH
SGPDFEEMVYYSVLALDVVSRNSDLSVAPSIQKGNNLNHAIGLGAMNLHGFLAKNEIFYD
CCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHCCCCCHHHHEEECCCEEEC
SEEALDFTNIFFYTLAFSAFKASEKLAKKYGPFSGFEKTKFASGEYFDKYTKVSPNTFVP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCC
KTHKIKELFAKYQVEIPTQKDIHLVEKIKKTGIANSHLMAIAPTGSISYLSSCTPSLQPV
CHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHCCCCCCCE
VAPVEIRKEGKLGRIYVPSYKLSPKTYKFYQNGAYELGPIPIINIVSEAQKHVDQAISMT
ECCCEECCCCCCCEEEECCEECCCCCEEHHCCCCEECCCCHHHHHHHHHHHHHHHHHEEE
LFMTDKATTRDLNRAYIYAFKKNCKSIYYVRIRQDVLEDSENYETCQSCQI
EEEECCCHHHCCCHHEEEEEHHCCCEEEEEEEEHHHHCCCCCCHHHHHCCC
>Mature Secondary Structure
MNFDKNQEKSYLSLNANSKIFANHPDSHKFDLLAAEKYINEEINPKFKFFNSFKERIEFL
CCCCCCCCCCEEEECCCCEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
VQNNYYDPKVLGQYSFQELEKLNTKASYNHFFSSFMGAFKFYSTYGLKSNDNLVYFEHFP
HHCCCCCCHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCC
DRVLLNSLFLGKGDFQKAEKILEQIMLGRFQPATPTFLNAGKLNRGEFVSCYLIRVEDNM
CHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCEEEEEEEEEECCH
ESISRAITTSLQLSKRGGGVSVLLSNLREQGAPIKNIENQATGVIPVMKILEDSFSYANQ
HHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LGQRQGAGAVYLNVHHPDILAFLDTKRENADEKIRIKSLSLGIIIPNITFELAKNNEKMA
HHHCCCCCEEEEEECCCCEEEEEECCCCCCCCEEEEEEEEEEEEECCCEEEEECCCCCEE
LFSVYDVEKVYQKAFSDISISEKYYQMLANPEIKKTFISARKLFKIIAELHFESGYPYIL
EEEHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
FEDIVNKENAHPDRVVMSNLCSEITQPSTPSTFLPDLSFKEIGKDICCNLGSLNIDKAME
EHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCHHHHCCCCCCCHHHHHH
SGPDFEEMVYYSVLALDVVSRNSDLSVAPSIQKGNNLNHAIGLGAMNLHGFLAKNEIFYD
CCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHCCCCCHHHHEEECCCEEEC
SEEALDFTNIFFYTLAFSAFKASEKLAKKYGPFSGFEKTKFASGEYFDKYTKVSPNTFVP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCC
KTHKIKELFAKYQVEIPTQKDIHLVEKIKKTGIANSHLMAIAPTGSISYLSSCTPSLQPV
CHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHCCCCCCCE
VAPVEIRKEGKLGRIYVPSYKLSPKTYKFYQNGAYELGPIPIINIVSEAQKHVDQAISMT
ECCCEECCCCCCCEEEECCEECCCCCEEHHCCCCEECCCCHHHHHHHHHHHHHHHHHEEE
LFMTDKATTRDLNRAYIYAFKKNCKSIYYVRIRQDVLEDSENYETCQSCQI
EEEECCCHHHCCCHHEEEEEHHCCCEEEEEEEEHHHHCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]