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The map label for this gene is trmE [H]

Identifier: 71893561

GI number: 71893561

Start: 231187

End: 232515

Strand: Reverse

Name: trmE [H]

Synonym: MHJ_0205

Alternate gene names: 71893561

Gene position: 232515-231187 (Counterclockwise)

Preceding gene: 71893562

Following gene: 71893552

Centisome position: 25.91

GC content: 31.3

Gene sequence:

>1329_bases
TTGCTTTCTGATACAATTTGTGCAATCGCTTCTGGGCAAATAAACCAGGCAATTTCAATCATTCGGATTTCTGGCCCTAA
TGCCTTTAAAATTATGGAAAAAATTTTTTTAGGTAAAGTCGGAAGGTCAATGGAGATAACTTTTGGTTGAATTCATGATG
ATAACCAAAAAATTGACCAAGTTTTAGTGCTCTGATTTGCTGGAAATAAAAATTTTGTCGGTGAAGACACTGTAGAAATT
AATGCTCACGGCGGTGTTCTAAATACAAATTTAATTCTTGAGCTTATTTTAAAAACCAAACTCGCCCGCCTTGCAAATCC
AGGTGAGTTTAGTCTCCGCGCTTTTTTAAATGGGAAAATTGATCTAGTAAAAGCCCAAGCCATTAATGATCTAATACATG
CTGAGGTAAAAGTTCAACATCAAGCAGCTTTAAATCAATTTTTAGGAAAAAGTTCTAATTTTATTAAAAATTTAATTGAA
AAAATTGAGGAAATTATCGGGATAATTGAAGTAAATATTGACTATCCAGAATATGATGATGTTGAAATTTTAACCTCAGA
TGTGCTTTTGCCAAGAATTAACCAATTATTAGCCGATTTTGATCAGTTAATAAAAATCGCAAATAATTCGCGCCTGATTT
ATCAAGGGATAAAAACTTGTCTTGTCGGAGCGCCCAACAGCGGAAAATCCTCACTTTTAAATATTTTAATTAACGAAAAT
AAGGCAATTATCAGCGAAATTCCAGGCACTACACGCGATGTTGTTGAGGGAAATTTTGTCCTTGACGGTCTTCTTTTTAA
ACTTTTTGATACCGCCGGAATTAGAAAAACTACTGAAAAAATTGAACAAATCGGTATTGAAAAATCTTATGAAAGTATCA
AAAAAGCGGATTTAATTCTGCACATTATTGATGCTAGTGAAAAAAACAGGCAAAATTTAGATCTAAAAGCAAAAACGCGA
CCTGATCAGATTTATCTTAAAATTTATAATAAATCAGATCTTCTTGAAAATCAAGAGGAATTTAAAGATGAAATTTTAAT
CAGTGCAAAATATCAAAAAATTGAAAATTTACTTGAAAAAATCAAGTCAATTTTTGCTTTTTTAGGAAAAAACAAAGAAT
TTGTTGCTAATTCCTTCCAAATTTCGCAAATTGAGCTAGGAAAATTAGCAATTCTAGATGCAAAAACCAGTTTAGAATCT
GGTTTTGGTCCTGAAATTGCAATTGTTGATCTCAGAATTGCCTGAAAAGAATTAAAAACTATTTTTGGCCGAGTTGATGA
TGAAAATCTTCTTGATTCAATTTTTTCAAAATTTTGCCTAGGAAAATAG

Upstream 100 bases:

>100_bases
AGTGCAATTTTTTTTCAAGGGAATTTGGCTGAATATGAGAAAAAAATAGGAATTTAGCTTTAATTTTTTCTATTTTTTAT
TTTTTATAGGAGAATTTAAG

Downstream 100 bases:

>100_bases
CTCTTCTTGTTTTTCTAAAAGTGATTTTATCGTTTTGGCAATTGATCCGGAGTTACAATTGCCAATTTTTTGATAAGCAA
CGCTTAACAAATCAGGGGTT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MLSDTICAIASGQINQAISIIRISGPNAFKIMEKIFLGKVGRSMEITFGWIHDDNQKIDQVLVLWFAGNKNFVGEDTVEI
NAHGGVLNTNLILELILKTKLARLANPGEFSLRAFLNGKIDLVKAQAINDLIHAEVKVQHQAALNQFLGKSSNFIKNLIE
KIEEIIGIIEVNIDYPEYDDVEILTSDVLLPRINQLLADFDQLIKIANNSRLIYQGIKTCLVGAPNSGKSSLLNILINEN
KAIISEIPGTTRDVVEGNFVLDGLLFKLFDTAGIRKTTEKIEQIGIEKSYESIKKADLILHIIDASEKNRQNLDLKAKTR
PDQIYLKIYNKSDLLENQEEFKDEILISAKYQKIENLLEKIKSIFAFLGKNKEFVANSFQISQIELGKLAILDAKTSLES
GFGPEIAIVDLRIAWKELKTIFGRVDDENLLDSIFSKFCLGK

Sequences:

>Translated_442_residues
MLSDTICAIASGQINQAISIIRISGPNAFKIMEKIFLGKVGRSMEITFG*IHDDNQKIDQVLVL*FAGNKNFVGEDTVEI
NAHGGVLNTNLILELILKTKLARLANPGEFSLRAFLNGKIDLVKAQAINDLIHAEVKVQHQAALNQFLGKSSNFIKNLIE
KIEEIIGIIEVNIDYPEYDDVEILTSDVLLPRINQLLADFDQLIKIANNSRLIYQGIKTCLVGAPNSGKSSLLNILINEN
KAIISEIPGTTRDVVEGNFVLDGLLFKLFDTAGIRKTTEKIEQIGIEKSYESIKKADLILHIIDASEKNRQNLDLKAKTR
PDQIYLKIYNKSDLLENQEEFKDEILISAKYQKIENLLEKIKSIFAFLGKNKEFVANSFQISQIELGKLAILDAKTSLES
GFGPEIAIVDLRIA*KELKTIFGRVDDENLLDSIFSKFCLGK
>Mature_442_residues
MLSDTICAIASGQINQAISIIRISGPNAFKIMEKIFLGKVGRSMEITFG*IHDDNQKIDQVLVL*FAGNKNFVGEDTVEI
NAHGGVLNTNLILELILKTKLARLANPGEFSLRAFLNGKIDLVKAQAINDLIHAEVKVQHQAALNQFLGKSSNFIKNLIE
KIEEIIGIIEVNIDYPEYDDVEILTSDVLLPRINQLLADFDQLIKIANNSRLIYQGIKTCLVGAPNSGKSSLLNILINEN
KAIISEIPGTTRDVVEGNFVLDGLLFKLFDTAGIRKTTEKIEQIGIEKSYESIKKADLILHIIDASEKNRQNLDLKAKTR
PDQIYLKIYNKSDLLENQEEFKDEILISAKYQKIENLLEKIKSIFAFLGKNKEFVANSFQISQIELGKLAILDAKTSLES
GFGPEIAIVDLRIA*KELKTIFGRVDDENLLDSIFSKFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=462, Percent_Identity=29.8701298701299, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI193082988, Length=494, Percent_Identity=28.1376518218623, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI193082993, Length=451, Percent_Identity=29.2682926829268, Blast_Score=179, Evalue=5e-45,
Organism=Escherichia coli, GI2367268, Length=456, Percent_Identity=32.8947368421053, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI87082120, Length=94, Percent_Identity=38.2978723404255, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17507259, Length=460, Percent_Identity=28.0434782608696, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6323665, Length=491, Percent_Identity=31.3645621181263, Blast_Score=175, Evalue=2e-44,
Organism=Drosophila melanogaster, GI45550813, Length=483, Percent_Identity=27.9503105590062, Blast_Score=170, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 48904; Mature: 48904

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSDTICAIASGQINQAISIIRISGPNAFKIMEKIFLGKVGRSMEITFGIHDDNQKIDQV
CCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEECCCHHHCEE
LVLFAGNKNFVGEDTVEINAHGGVLNTNLILELILKTKLARLANPGEFSLRAFLNGKIDL
EEEEECCCCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEE
VKAQAINDLIHAEVKVQHQAALNQFLGKSSNFIKNLIEKIEEIIGIIEVNIDYPEYDDVE
EHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCEEEEEECCCCCCCHH
ILTSDVLLPRINQLLADFDQLIKIANNSRLIYQGIKTCLVGAPNSGKSSLLNILINENKA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHEECCCCCCHHHHEEEEECCCCH
IISEIPGTTRDVVEGNFVLDGLLFKLFDTAGIRKTTEKIEQIGIEKSYESIKKADLILHI
HHHHCCCCHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHEEEEE
IDASEKNRQNLDLKAKTRPDQIYLKIYNKSDLLENQEEFKDEILISAKYQKIENLLEKIK
ECCCCCCCCCCCEEECCCCCEEEEEEECCHHHHCCHHHHHHHHEEHHHHHHHHHHHHHHH
SIFAFLGKNKEFVANSFQISQIELGKLAILDAKTSLESGFGPEIAIVDLRIAKELKTIFG
HHHHHHCCCHHHEECCEEEEEEEECEEEEEECHHHHCCCCCCCEEEEEHHHHHHHHHHHC
RVDDENLLDSIFSKFCLGK
CCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLSDTICAIASGQINQAISIIRISGPNAFKIMEKIFLGKVGRSMEITFGIHDDNQKIDQV
CCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEECCCHHHCEE
LVLFAGNKNFVGEDTVEINAHGGVLNTNLILELILKTKLARLANPGEFSLRAFLNGKIDL
EEEEECCCCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEE
VKAQAINDLIHAEVKVQHQAALNQFLGKSSNFIKNLIEKIEEIIGIIEVNIDYPEYDDVE
EHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCEEEEEECCCCCCCHH
ILTSDVLLPRINQLLADFDQLIKIANNSRLIYQGIKTCLVGAPNSGKSSLLNILINENKA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHEECCCCCCHHHHEEEEECCCCH
IISEIPGTTRDVVEGNFVLDGLLFKLFDTAGIRKTTEKIEQIGIEKSYESIKKADLILHI
HHHHCCCCHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHEEEEE
IDASEKNRQNLDLKAKTRPDQIYLKIYNKSDLLENQEEFKDEILISAKYQKIENLLEKIK
ECCCCCCCCCCCEEECCCCCEEEEEEECCHHHHCCHHHHHHHHEEHHHHHHHHHHHHHHH
SIFAFLGKNKEFVANSFQISQIELGKLAILDAKTSLESGFGPEIAIVDLRIAKELKTIFG
HHHHHHCCCHHHEECCEEEEEEEECEEEEEECHHHHCCCCCCCEEEEEHHHHHHHHHHHC
RVDDENLLDSIFSKFCLGK
CCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA