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The map label for this gene is rsmE [H]

Identifier: 71893552

GI number: 71893552

Start: 220882

End: 221565

Strand: Reverse

Name: rsmE [H]

Synonym: MHJ_0196

Alternate gene names: 71893552

Gene position: 221565-220882 (Counterclockwise)

Preceding gene: 71893561

Following gene: 71893547

Centisome position: 24.69

GC content: 29.24

Gene sequence:

>684_bases
ATGTATCGCTTTTTTGTCAATGAAAAACAAGAAAATTTCTTTATCTTAACAAATATAACCTTAAACCATATTAAAACTGT
AAGAATTAGAAATGAAAATTTTATTTGTGTTTATCAAAATGAATTCTACCTTGTGAGATTAGTGCCTAATTCAAACAAAG
CAGAAATAATTGAAAAAATTAAGGGGAATAACGAACCAAAAAACAAAGTTGTTCTCGCGCTTGCAATTTTGAAGACAAAA
TCATTTGAATTTGCAATCCAGAAAGCTGTTGAAATTGGGGTTAGTGAAATTTGACCTTTTTATAGTAAAAATGTTTCCCA
AAAAATAAGCGGAGATCTTGAAAAAAAACTCAAAAGATGAGAACAAATTTGTCTTCATAGCGCCCAACAAAGTTTCCGCA
ATTTGATCCCGAAAATAAATTTACCTATAAATTATAAGGATCTTTTAGAACAAGCCAAAAATTTTCCAGTAAAATTAATC
AGTTTTGAAAGAGCAAAAAATAATATAAATATCCCTGATAACCCCCAAAATACAATTATAATCATTGGGCCTGAAGGTGG
GTTTGATGATGTTGAAATCCAACAAGCAGAAAAATTAGGGTTTCAAAGTATCACACTCGGGAAAAGAATTCTTAGATCGG
AGACAGCAGCAATTTTTCTTTTAACAAAATGTATAAAAGATTAA

Upstream 100 bases:

>100_bases
ATTTTGTTTGCTGATAATTTTTCCATAGTTTTTCCTTCTTTTTTAATAATTTTAAACTATTTTTTAAATAGTTTATCAAT
TTTCCTTTAAAATTTTTATT

Downstream 100 bases:

>100_bases
TTTTGGCTAGAAATGCAAATTTTTATAAAAATTTGCATTATTAAACATCCTTTTTCAACGAAAAAATAGATTTAATAATA
TATCACCAATATTATCAAAA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MYRFFVNEKQENFFILTNITLNHIKTVRIRNENFICVYQNEFYLVRLVPNSNKAEIIEKIKGNNEPKNKVVLALAILKTK
SFEFAIQKAVEIGVSEIWPFYSKNVSQKISGDLEKKLKRWEQICLHSAQQSFRNLIPKINLPINYKDLLEQAKNFPVKLI
SFERAKNNINIPDNPQNTIIIIGPEGGFDDVEIQQAEKLGFQSITLGKRILRSETAAIFLLTKCIKD

Sequences:

>Translated_227_residues
MYRFFVNEKQENFFILTNITLNHIKTVRIRNENFICVYQNEFYLVRLVPNSNKAEIIEKIKGNNEPKNKVVLALAILKTK
SFEFAIQKAVEIGVSEI*PFYSKNVSQKISGDLEKKLKR*EQICLHSAQQSFRNLIPKINLPINYKDLLEQAKNFPVKLI
SFERAKNNINIPDNPQNTIIIIGPEGGFDDVEIQQAEKLGFQSITLGKRILRSETAAIFLLTKCIKD
>Mature_227_residues
MYRFFVNEKQENFFILTNITLNHIKTVRIRNENFICVYQNEFYLVRLVPNSNKAEIIEKIKGNNEPKNKVVLALAILKTK
SFEFAIQKAVEIGVSEI*PFYSKNVSQKISGDLEKKLKR*EQICLHSAQQSFRNLIPKINLPINYKDLLEQAKNFPVKLI
SFERAKNNINIPDNPQNTIIIIGPEGGFDDVEIQQAEKLGFQSITLGKRILRSETAAIFLLTKCIKD

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=188, Percent_Identity=30.3191489361702, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 25879; Mature: 25879

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRFFVNEKQENFFILTNITLNHIKTVRIRNENFICVYQNEFYLVRLVPNSNKAEIIEKI
CEEEEECCCCCCEEEEEEEEECCEEEEEEECCCEEEEEECCEEEEEECCCCCHHHHHHHH
KGNNEPKNKVVLALAILKTKSFEFAIQKAVEIGVSEIPFYSKNVSQKISGDLEKKLKREQ
CCCCCCCHHEEEEEEEHHCCCHHHHHHHHHHCCHHHCCCCCCCHHHHHCCHHHHHHHHHH
ICLHSAQQSFRNLIPKINLPINYKDLLEQAKNFPVKLISFERAKNNINIPDNPQNTIIII
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCEEEEE
GPEGGFDDVEIQQAEKLGFQSITLGKRILRSETAAIFLLTKCIKD
CCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYRFFVNEKQENFFILTNITLNHIKTVRIRNENFICVYQNEFYLVRLVPNSNKAEIIEKI
CEEEEECCCCCCEEEEEEEEECCEEEEEEECCCEEEEEECCEEEEEECCCCCHHHHHHHH
KGNNEPKNKVVLALAILKTKSFEFAIQKAVEIGVSEIPFYSKNVSQKISGDLEKKLKREQ
CCCCCCCHHEEEEEEEHHCCCHHHHHHHHHHCCHHHCCCCCCCHHHHHCCHHHHHHHHHH
ICLHSAQQSFRNLIPKINLPINYKDLLEQAKNFPVKLISFERAKNNINIPDNPQNTIIII
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCEEEEE
GPEGGFDDVEIQQAEKLGFQSITLGKRILRSETAAIFLLTKCIKD
CCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9729120 [H]