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The map label for this gene is rsmD [H]

Identifier: 71893506

GI number: 71893506

Start: 186066

End: 186626

Strand: Reverse

Name: rsmD [H]

Synonym: MHJ_0150

Alternate gene names: 71893506

Gene position: 186626-186066 (Counterclockwise)

Preceding gene: 71893507

Following gene: 71893505

Centisome position: 20.8

GC content: 29.06

Gene sequence:

>561_bases
ATGATTAGAATTATTTCCGGAAATTATCGTGGTGCGGTTATTAAAAATCCTGAATTTTCAGTGGTGCGTCCAATGTCTAG
TCGTTGTCGTGAAGCAATTTTTTCCCAACTTCAATTTGAAATTCCTAATTCTAAGGTGCTTGATTTATTTGCCGGTACTG
GAGCGATTGGGCTGGAGGCGACTTCTCGTGGCGCTAAAAAGGTGGTAGCAACCGAATTAAACCAAAAAGCTTACCAAAAT
ATAGTAGATTTTTGTAAAAAATATAATATTAAAAATTATCAAATTTTTAATAAAAGTGCAATTTTTTTAATTAATGATCT
TAAAAATAAAAAATTTGACTTCATTTTCCTAGATCCTCCCCATAGTGAAGAAGAAATTACAAAAAAATGTTTCTGAAAAA
TTGCAGAAAATAATTTATTAGCAAAAAATGGACTTATAATTTATAAAACAAATTTAGGCTCTAAACTAATTCCGGACATT
TTTTCAATCCATCAAATAAAAAAATACGGTAAAAACAGCGTTTTTTTCCTAAAATATTACAATGAAGGTAAAAATGAGTA
A

Upstream 100 bases:

>100_bases
ATTTTTATTATATTTATTCTTTGTTTGATGTTTTAAAATTATTTTTAAATAAAAATTCATACATTTTTCTATAAACTTTT
GGTATAATTTTTAAAAATTT

Downstream 100 bases:

>100_bases
ATTAATAATTTTGTCTGGACCTTCAGGGGTTGGCAAGGGCACAATTGAATCACTTTTATTAAAAAATAAAAATTTATTAA
TAAAGTTAGCAATTTCTGCA

Product: putative DNA methylase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MIRIISGNYRGAVIKNPEFSVVRPMSSRCREAIFSQLQFEIPNSKVLDLFAGTGAIGLEATSRGAKKVVATELNQKAYQN
IVDFCKKYNIKNYQIFNKSAIFLINDLKNKKFDFIFLDPPHSEEEITKKCFWKIAENNLLAKNGLIIYKTNLGSKLIPDI
FSIHQIKKYGKNSVFFLKYYNEGKNE

Sequences:

>Translated_186_residues
MIRIISGNYRGAVIKNPEFSVVRPMSSRCREAIFSQLQFEIPNSKVLDLFAGTGAIGLEATSRGAKKVVATELNQKAYQN
IVDFCKKYNIKNYQIFNKSAIFLINDLKNKKFDFIFLDPPHSEEEITKKCF*KIAENNLLAKNGLIIYKTNLGSKLIPDI
FSIHQIKKYGKNSVFFLKYYNEGKNE
>Mature_186_residues
MIRIISGNYRGAVIKNPEFSVVRPMSSRCREAIFSQLQFEIPNSKVLDLFAGTGAIGLEATSRGAKKVVATELNQKAYQN
IVDFCKKYNIKNYQIFNKSAIFLINDLKNKKFDFIFLDPPHSEEEITKKCF*KIAENNLLAKNGLIIYKTNLGSKLIPDI
FSIHQIKKYGKNSVFFLKYYNEGKNE

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=186, Percent_Identity=29.5698924731183, Blast_Score=93, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 21156; Mature: 21156

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRIISGNYRGAVIKNPEFSVVRPMSSRCREAIFSQLQFEIPNSKVLDLFAGTGAIGLEA
CEEEEECCCCEEEEECCCCEEECCHHHHHHHHHHHHHEEECCCCCEEEEEECCCCEEECC
TSRGAKKVVATELNQKAYQNIVDFCKKYNIKNYQIFNKSAIFLINDLKNKKFDFIFLDPP
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCCCCCCEEEECCC
HSEEEITKKCFKIAENNLLAKNGLIIYKTNLGSKLIPDIFSIHQIKKYGKNSVFFLKYYN
CCHHHHHHHHHHHHCCCEEECCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEEC
EGKNE
CCCCC
>Mature Secondary Structure
MIRIISGNYRGAVIKNPEFSVVRPMSSRCREAIFSQLQFEIPNSKVLDLFAGTGAIGLEA
CEEEEECCCCEEEEECCCCEEECCHHHHHHHHHHHHHEEECCCCCEEEEEECCCCEEECC
TSRGAKKVVATELNQKAYQNIVDFCKKYNIKNYQIFNKSAIFLINDLKNKKFDFIFLDPP
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCCCCCCEEEECCC
HSEEEITKKCFKIAENNLLAKNGLIIYKTNLGSKLIPDIFSIHQIKKYGKNSVFFLKYYN
CCHHHHHHHHHHHHCCCEEECCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEEC
EGKNE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]