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The map label for this gene is yhhF [H]

Identifier: 71892396

GI number: 71892396

Start: 786893

End: 787480

Strand: Direct

Name: yhhF [H]

Synonym: BPEN_655

Alternate gene names: 71892396

Gene position: 786893-787480 (Clockwise)

Preceding gene: 71892385

Following gene: NA

Centisome position: 99.4

GC content: 28.74

Gene sequence:

>588_bases
GTGAAAAATAAATTACATTTAGGCGTATCCAGAAAAATTAAGATTATTGGCGGTAAATGGAAGGGAAGAAACATACCAAT
TACTCGGCATTCTAAGATACGTCCAACTACTAATCGTATGCGTGAAATATTATTTAGTTGGCTTAATCCTATTATTTCTA
GAGCGGTGTGTTTAGATTGTTTTGCTGGAAGCGGTGCATTAGGTTTAGAATCATTGTCTAGAGGAGCTAATAAAGTAACT
TTTTTAGATAATAATCATATTTGTATTACTGCATTGATAAAAGTCATGCAGTCTTTTTCAGCATATAACAGTGAAATAAT
ATATACTGATTGTCGTTCTTGGTTACAACAATCGACTACGACTTATAATGTCATCTTTCTTGATCCTCCTTTTCAAAATA
ATATTATACCTGAAGTTATTTTTTTATTAGAAAGGTCCAATCTATTGGAAAAAGAATCTTGGATTTATATAGAAACTTCA
AAAAAAAGAAACATTTTTAATACTAGTATAATTCCGAATTATTGGATTTTATATCATAAAAAAATTACTCGGAATGTCAT
GTATTATCTTTTTTTAAGAAGATCATAG

Upstream 100 bases:

>100_bases
AATTGATGATTCATGACTCATAATAAAAATCTTTTCATTTCAAAAAATAAAAATATCTTGTGAATTATTATTTTAATATA
TACAGAAATATAGTGGATGT

Downstream 100 bases:

>100_bases
AATGTTTGAATATAATAAAAACACTACTCAAAACATGTTATTTCACACGTCTACCACAATTTGATGTAGCTTTTTTATAC
ATCAAAAAATTGCATTAACC

Product: methyltransferase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MKNKLHLGVSRKIKIIGGKWKGRNIPITRHSKIRPTTNRMREILFSWLNPIISRAVCLDCFAGSGALGLESLSRGANKVT
FLDNNHICITALIKVMQSFSAYNSEIIYTDCRSWLQQSTTTYNVIFLDPPFQNNIIPEVIFLLERSNLLEKESWIYIETS
KKRNIFNTSIIPNYWILYHKKITRNVMYYLFLRRS

Sequences:

>Translated_195_residues
MKNKLHLGVSRKIKIIGGKWKGRNIPITRHSKIRPTTNRMREILFSWLNPIISRAVCLDCFAGSGALGLESLSRGANKVT
FLDNNHICITALIKVMQSFSAYNSEIIYTDCRSWLQQSTTTYNVIFLDPPFQNNIIPEVIFLLERSNLLEKESWIYIETS
KKRNIFNTSIIPNYWILYHKKITRNVMYYLFLRRS
>Mature_195_residues
MKNKLHLGVSRKIKIIGGKWKGRNIPITRHSKIRPTTNRMREILFSWLNPIISRAVCLDCFAGSGALGLESLSRGANKVT
FLDNNHICITALIKVMQSFSAYNSEIIYTDCRSWLQQSTTTYNVIFLDPPFQNNIIPEVIFLLERSNLLEKESWIYIETS
KKRNIFNTSIIPNYWILYHKKITRNVMYYLFLRRS

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=195, Percent_Identity=41.025641025641, Blast_Score=169, Evalue=9e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 22807; Mature: 22807

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKLHLGVSRKIKIIGGKWKGRNIPITRHSKIRPTTNRMREILFSWLNPIISRAVCLDC
CCCCEEECCCEEEEEEECEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FAGSGALGLESLSRGANKVTFLDNNHICITALIKVMQSFSAYNSEIIYTDCRSWLQQSTT
HCCCCCCCHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCC
TYNVIFLDPPFQNNIIPEVIFLLERSNLLEKESWIYIETSKKRNIFNTSIIPNYWILYHK
EEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEECCCCCCEEEEEHH
KITRNVMYYLFLRRS
HHHHHEEEEEEEECC
>Mature Secondary Structure
MKNKLHLGVSRKIKIIGGKWKGRNIPITRHSKIRPTTNRMREILFSWLNPIISRAVCLDC
CCCCEEECCCEEEEEEECEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FAGSGALGLESLSRGANKVTFLDNNHICITALIKVMQSFSAYNSEIIYTDCRSWLQQSTT
HCCCCCCCHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCC
TYNVIFLDPPFQNNIIPEVIFLLERSNLLEKESWIYIETSKKRNIFNTSIIPNYWILYHK
EEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEECCCCCCEEEEEHH
KITRNVMYYLFLRRS
HHHHHEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]