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The map label for this gene is metB [H]

Identifier: 71892361

GI number: 71892361

Start: 741619

End: 742767

Strand: Reverse

Name: metB [H]

Synonym: BPEN_620

Alternate gene names: 71892361

Gene position: 742767-741619 (Counterclockwise)

Preceding gene: 71892362

Following gene: 71892360

Centisome position: 93.82

GC content: 33.07

Gene sequence:

>1149_bases
ATGAATATAAAAAAATCTACAATTAGCGTTAGAAGTGGTTTAAATAGTGATGAGCAACATGGATGTGTCGTACCTCCTAT
TACTCTTTCAACTACTTATAATTTTTTTGGATTAAATCAGCCTCGTTCTTATGATTATTCACGGCGTAAAAATCCTACTC
GTGATGTAACTCAACAAACTTTATCAGATTTAGAATATGGAAAAAATGCGATTATGACAAGTAGCGGAATGTCTGCTATT
TATTTAATATGTTCTGCTTTGCTTGAACCAAACGATTTATTAATAGCACCTTATGATTGCTATGGAGGTACTTATCGATT
ATTGGATGCGTTAAACAAAAAAGGTGCGTGCAAAGTATTATTTATTGATCAAAATGATACACAGATTTTATTAAAATCAT
TAGCATATAAACCTAAATTAATTTTTATAGAAACCCCAAGTAATCCTATGCTCCGAATAGTAGATATTCATACTCTTTGC
AACCTCCAAAAAGGTATCTTATATGTTGTAGATAATACATTTATGACTCCAGTATTTCAAAATCCATTAAATTTAGGTGC
AGATTTAGTAGTACATTCTTGTAGTAAGTATTTGAATGGACATTCAGATTTAATAGCAGGAGTAGTAATATCTAAGGATA
CATATATTTCCGAAAAATTAACTTGGTGGGGCAATACGCTCGGTATTACTGGAAGTGCCTTTGATAGTTATCAGTTGTTG
CGCGGTATGCGTACTTTAATGCCGCGTGTTTACCAGCAACAAAATAATACTAAAAGAATTATTGCTTTTTGTCAAAAACA
ACCCCAAATTAATGCATTATATTATCCAGGATTACCCAATCACCCTGGATATAACATTGTTTGTAAACAACAAAGCGGAT
TCGGATCAATATTTAGCTTCGAACTGAAGGGCGCTAAAAATGTTTTACTACAATTTTTACGATCATTAAAATTATTTACA
TTAGCAGAATCGTTCGGGGGTGTTGAAAGTTTAATTGCTCATCCAGCCACTATGACGCATGCTGCTATGCCAGAAAATGC
TAGAAAACGCGCTGGAATCAGTGATATGTTGCTTCGTGTTTCAGTAGGACTAGAAGATAGCGATGATCTTATTACTGATT
TAGAACAGGCATTTGCATCAATACAATAA

Upstream 100 bases:

>100_bases
TATTAAATCTGGTCAGTAAAAATATTTTCGTACACATAAAAATTTCTATACTTATAACGTACCGGTGTTTGTAGAAATAT
AGGGCAGTTTAGTTATTATT

Downstream 100 bases:

>100_bases
GGTCTCTCCATATTATTATTATACGCATTATTAATATTTAATCAGTCTTATTTTTAACAAATAATAATGGACGATTTATT
ATAAAATTTTGTTAATTAAT

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MNIKKSTISVRSGLNSDEQHGCVVPPITLSTTYNFFGLNQPRSYDYSRRKNPTRDVTQQTLSDLEYGKNAIMTSSGMSAI
YLICSALLEPNDLLIAPYDCYGGTYRLLDALNKKGACKVLFIDQNDTQILLKSLAYKPKLIFIETPSNPMLRIVDIHTLC
NLQKGILYVVDNTFMTPVFQNPLNLGADLVVHSCSKYLNGHSDLIAGVVISKDTYISEKLTWWGNTLGITGSAFDSYQLL
RGMRTLMPRVYQQQNNTKRIIAFCQKQPQINALYYPGLPNHPGYNIVCKQQSGFGSIFSFELKGAKNVLLQFLRSLKLFT
LAESFGGVESLIAHPATMTHAAMPENARKRAGISDMLLRVSVGLEDSDDLITDLEQAFASIQ

Sequences:

>Translated_382_residues
MNIKKSTISVRSGLNSDEQHGCVVPPITLSTTYNFFGLNQPRSYDYSRRKNPTRDVTQQTLSDLEYGKNAIMTSSGMSAI
YLICSALLEPNDLLIAPYDCYGGTYRLLDALNKKGACKVLFIDQNDTQILLKSLAYKPKLIFIETPSNPMLRIVDIHTLC
NLQKGILYVVDNTFMTPVFQNPLNLGADLVVHSCSKYLNGHSDLIAGVVISKDTYISEKLTWWGNTLGITGSAFDSYQLL
RGMRTLMPRVYQQQNNTKRIIAFCQKQPQINALYYPGLPNHPGYNIVCKQQSGFGSIFSFELKGAKNVLLQFLRSLKLFT
LAESFGGVESLIAHPATMTHAAMPENARKRAGISDMLLRVSVGLEDSDDLITDLEQAFASIQ
>Mature_382_residues
MNIKKSTISVRSGLNSDEQHGCVVPPITLSTTYNFFGLNQPRSYDYSRRKNPTRDVTQQTLSDLEYGKNAIMTSSGMSAI
YLICSALLEPNDLLIAPYDCYGGTYRLLDALNKKGACKVLFIDQNDTQILLKSLAYKPKLIFIETPSNPMLRIVDIHTLC
NLQKGILYVVDNTFMTPVFQNPLNLGADLVVHSCSKYLNGHSDLIAGVVISKDTYISEKLTWWGNTLGITGSAFDSYQLL
RGMRTLMPRVYQQQNNTKRIIAFCQKQPQINALYYPGLPNHPGYNIVCKQQSGFGSIFSFELKGAKNVLLQFLRSLKLFT
LAESFGGVESLIAHPATMTHAAMPENARKRAGISDMLLRVSVGLEDSDDLITDLEQAFASIQ

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=344, Percent_Identity=39.2441860465116, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI299473759, Length=344, Percent_Identity=35.7558139534884, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI25453487, Length=341, Percent_Identity=34.0175953079179, Blast_Score=194, Evalue=1e-49,
Organism=Escherichia coli, GI1790375, Length=380, Percent_Identity=59.2105263157895, Blast_Score=500, Evalue=1e-143,
Organism=Escherichia coli, GI1789383, Length=343, Percent_Identity=27.6967930029155, Blast_Score=137, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17560056, Length=348, Percent_Identity=42.2413793103448, Blast_Score=271, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI17538055, Length=349, Percent_Identity=41.5472779369628, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI71980708, Length=371, Percent_Identity=31.5363881401617, Blast_Score=187, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6319307, Length=387, Percent_Identity=38.2428940568475, Blast_Score=271, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6321254, Length=409, Percent_Identity=34.4743276283619, Blast_Score=193, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6323333, Length=433, Percent_Identity=27.0207852193995, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6321904, Length=393, Percent_Identity=30.0254452926209, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6321170, Length=323, Percent_Identity=26.9349845201238, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19922554, Length=378, Percent_Identity=40.2116402116402, Blast_Score=296, Evalue=2e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR011821
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 42535; Mature: 42535

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKKSTISVRSGLNSDEQHGCVVPPITLSTTYNFFGLNQPRSYDYSRRKNPTRDVTQQT
CCCCCHHHHHHCCCCCCCCCCCEECCEEEECEEEEEECCCCCCCCCHHCCCCCHHHHHHH
LSDLEYGKNAIMTSSGMSAIYLICSALLEPNDLLIAPYDCYGGTYRLLDALNKKGACKVL
HHHHHHCCCEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCCCEEEE
FIDQNDTQILLKSLAYKPKLIFIETPSNPMLRIVDIHTLCNLQKGILYVVDNTFMTPVFQ
EECCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEEHHHHHHHCCCEEEEECCCEECHHHC
NPLNLGADLVVHSCSKYLNGHSDLIAGVVISKDTYISEKLTWWGNTLGITGSAFDSYQLL
CCHHCCHHHHHHHHHHHHCCCHHHHEEEEEECCCHHHHHHEECCCCCCCCCCCHHHHHHH
RGMRTLMPRVYQQQNNTKRIIAFCQKQPQINALYYPGLPNHPGYNIVCKQQSGFGSIFSF
HHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEEECCCCCCCEEEE
ELKGAKNVLLQFLRSLKLFTLAESFGGVESLIAHPATMTHAAMPENARKRAGISDMLLRV
EECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHCCCCHHHHHCCHHHHHEEE
SVGLEDSDDLITDLEQAFASIQ
ECCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNIKKSTISVRSGLNSDEQHGCVVPPITLSTTYNFFGLNQPRSYDYSRRKNPTRDVTQQT
CCCCCHHHHHHCCCCCCCCCCCEECCEEEECEEEEEECCCCCCCCCHHCCCCCHHHHHHH
LSDLEYGKNAIMTSSGMSAIYLICSALLEPNDLLIAPYDCYGGTYRLLDALNKKGACKVL
HHHHHHCCCEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCCCEEEE
FIDQNDTQILLKSLAYKPKLIFIETPSNPMLRIVDIHTLCNLQKGILYVVDNTFMTPVFQ
EECCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEEHHHHHHHCCCEEEEECCCEECHHHC
NPLNLGADLVVHSCSKYLNGHSDLIAGVVISKDTYISEKLTWWGNTLGITGSAFDSYQLL
CCHHCCHHHHHHHHHHHHCCCHHHHEEEEEECCCHHHHHHEECCCCCCCCCCCHHHHHHH
RGMRTLMPRVYQQQNNTKRIIAFCQKQPQINALYYPGLPNHPGYNIVCKQQSGFGSIFSF
HHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEEECCCCCCCEEEE
ELKGAKNVLLQFLRSLKLFTLAESFGGVESLIAHPATMTHAAMPENARKRAGISDMLLRV
EECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHCCCCHHHHHCCHHHHHEEE
SVGLEDSDDLITDLEQAFASIQ
ECCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6361020; 8346018; 9278503; 3307782; 9843488 [H]