Click here to switch to the map view.

The map label for this gene is metF [H]

Identifier: 71892360

GI number: 71892360

Start: 740314

End: 741207

Strand: Reverse

Name: metF [H]

Synonym: BPEN_619

Alternate gene names: 71892360

Gene position: 741207-740314 (Counterclockwise)

Preceding gene: 71892361

Following gene: 71892359

Centisome position: 93.63

GC content: 29.31

Gene sequence:

>894_bases
ATGAGTATGTTCCATGCCACCCAACAAGCAGTATTAAATCAATATTTAGCTGAATTACAAGGAAATATTAATGTTTCATT
TGAATTTTTTCCTCCACGTACCGATGAAATGGAACAAATATTATGGAAAACTATAAACAAATTAAGCAAATTGAATCCTA
TTTTTGTTTCTGTAACATATAGCGCTCATTCTGGAACACGAAATTATACTGATAAAACAATTAAAGACATAAAAAAACGT
ACTGGATTAATAGCAGCTCCTCATTTAACATGTATTAACGAAACTCCTCAAGCATTACAAACTATTGCTCAAGAATATTG
GAATAATGGTATTCATAATATAGTTGCATTAAGAGGCGATCAAATAAAAAAAAACTATCAATCGTCTATGTATGCTTCAG
ATTTAGTATATTTATTAAAAAAAATTGGAAATTTTGATATAGCAGTAGCTGCTTATCCAGAAGTACATCCAGAAGCAAAA
AACGCACAATCAGATTTAATTAATTTAAAGAAAAAAATAGATGCCGGAGCGAATCGTGCAATCACTCAATTTTTTTTTGA
CGTAGAACAATATTTAAGATTTAGAGATCTTTGTGTGTCGGTTGGGATTGATATAGAAATTATTCCTGGAATATTGCCTA
TCTTTAATTTTCGTCAATTACAACATTTTATCACTTTTACTAAAGTGAAGATACCGCATTGGATATATGTAATTTTTCAT
GGATTAGATCATGACTTGGAAACACAGAAAATGCTTGGAACATTTGTAGCAATAGACATGATCAGAGTCCTTCTTAAGGA
AGGAGTAAGAAATTTTCATTTCTACACTCTTAATAGATCAGATTTAACATATGCAATATGTCATACTTTGGGAATAAAAA
ATAACAAAATATAA

Upstream 100 bases:

>100_bases
ATTATTTATGTAATGATGATTTTTATTTAAAACTAGCATACGGTCTATATAATGTATATAGGTCTATAAGCATTTATTTT
ATAGCTTCAAGAAGGAAAAC

Downstream 100 bases:

>100_bases
TGTATGAATAATATTTCAAAATTAATTAAATGATTACGTCCATCACAAATCATAATTTAAATATGATAAGTTTATTTCTT
AGAGTCTTTTAAATATATGT

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSMFHATQQAVLNQYLAELQGNINVSFEFFPPRTDEMEQILWKTINKLSKLNPIFVSVTYSAHSGTRNYTDKTIKDIKKR
TGLIAAPHLTCINETPQALQTIAQEYWNNGIHNIVALRGDQIKKNYQSSMYASDLVYLLKKIGNFDIAVAAYPEVHPEAK
NAQSDLINLKKKIDAGANRAITQFFFDVEQYLRFRDLCVSVGIDIEIIPGILPIFNFRQLQHFITFTKVKIPHWIYVIFH
GLDHDLETQKMLGTFVAIDMIRVLLKEGVRNFHFYTLNRSDLTYAICHTLGIKNNKI

Sequences:

>Translated_297_residues
MSMFHATQQAVLNQYLAELQGNINVSFEFFPPRTDEMEQILWKTINKLSKLNPIFVSVTYSAHSGTRNYTDKTIKDIKKR
TGLIAAPHLTCINETPQALQTIAQEYWNNGIHNIVALRGDQIKKNYQSSMYASDLVYLLKKIGNFDIAVAAYPEVHPEAK
NAQSDLINLKKKIDAGANRAITQFFFDVEQYLRFRDLCVSVGIDIEIIPGILPIFNFRQLQHFITFTKVKIPHWIYVIFH
GLDHDLETQKMLGTFVAIDMIRVLLKEGVRNFHFYTLNRSDLTYAICHTLGIKNNKI
>Mature_296_residues
SMFHATQQAVLNQYLAELQGNINVSFEFFPPRTDEMEQILWKTINKLSKLNPIFVSVTYSAHSGTRNYTDKTIKDIKKRT
GLIAAPHLTCINETPQALQTIAQEYWNNGIHNIVALRGDQIKKNYQSSMYASDLVYLLKKIGNFDIAVAAYPEVHPEAKN
AQSDLINLKKKIDAGANRAITQFFFDVEQYLRFRDLCVSVGIDIEIIPGILPIFNFRQLQHFITFTKVKIPHWIYVIFHG
LDHDLETQKMLGTFVAIDMIRVLLKEGVRNFHFYTLNRSDLTYAICHTLGIKNNKI

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=285, Percent_Identity=32.280701754386, Blast_Score=152, Evalue=5e-37,
Organism=Escherichia coli, GI1790377, Length=293, Percent_Identity=65.8703071672355, Blast_Score=429, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI25147441, Length=280, Percent_Identity=31.0714285714286, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25147436, Length=280, Percent_Identity=31.0714285714286, Blast_Score=133, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6325234, Length=276, Percent_Identity=32.6086956521739, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6321313, Length=288, Percent_Identity=30.2083333333333, Blast_Score=125, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 34170; Mature: 34039

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMFHATQQAVLNQYLAELQGNINVSFEFFPPRTDEMEQILWKTINKLSKLNPIFVSVTY
CCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEEE
SAHSGTRNYTDKTIKDIKKRTGLIAAPHLTCINETPQALQTIAQEYWNNGIHNIVALRGD
ECCCCCCCCHHHHHHHHHHHCCCEECCCEEECCCCHHHHHHHHHHHHCCCCHHEEEECCH
QIKKNYQSSMYASDLVYLLKKIGNFDIAVAAYPEVHPEAKNAQSDLINLKKKIDAGANRA
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHH
ITQFFFDVEQYLRFRDLCVSVGIDIEIIPGILPIFNFRQLQHFITFTKVKIPHWIYVIFH
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHEECCCEEEEEEE
GLDHDLETQKMLGTFVAIDMIRVLLKEGVRNFHFYTLNRSDLTYAICHTLGIKNNKI
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SMFHATQQAVLNQYLAELQGNINVSFEFFPPRTDEMEQILWKTINKLSKLNPIFVSVTY
CCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEEE
SAHSGTRNYTDKTIKDIKKRTGLIAAPHLTCINETPQALQTIAQEYWNNGIHNIVALRGD
ECCCCCCCCHHHHHHHHHHHCCCEECCCEEECCCCHHHHHHHHHHHHCCCCHHEEEECCH
QIKKNYQSSMYASDLVYLLKKIGNFDIAVAAYPEVHPEAKNAQSDLINLKKKIDAGANRA
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHH
ITQFFFDVEQYLRFRDLCVSVGIDIEIIPGILPIFNFRQLQHFITFTKVKIPHWIYVIFH
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHEECCCEEEEEEE
GLDHDLETQKMLGTFVAIDMIRVLLKEGVRNFHFYTLNRSDLTYAICHTLGIKNNKI
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA