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The map label for this gene is purH [H]

Identifier: 71892324

GI number: 71892324

Start: 686483

End: 688117

Strand: Direct

Name: purH [H]

Synonym: BPEN_575

Alternate gene names: 71892324

Gene position: 686483-688117 (Clockwise)

Preceding gene: 71892323

Following gene: 71892342

Centisome position: 86.72

GC content: 32.6

Gene sequence:

>1635_bases
ATGATACAATCATCATTGTTAATTCGTCGTGTATTAATTAGTGTATTTGATAAATCCAATATTTTAGAATTTGCTCAGTC
ATTAATTCAACGGGGTATTCAATTATTATCTACTGGGGGTACGGCTCGAATATTATCTGATGCTGGATTGCCAGTAATCA
AGATTTCTGAGTATACTAAATTACCTGAAATTATGGACGGGCGCGTTAAAACTTTACACTATAAAGTGTATGCCGGAATT
CTTGGTAGACGAGGATTAGATGACGCTATCATGCGTCAATATAATATTCCGCCAATTGATATGGTAGTAGTTAATTTTTA
TTCATTTAATTCTTTGCTTGCAAAAAATAAAAAATATTCTCAAGAAGAAATGTTAGATCGTATTGATATCAGTGGCCCAA
GTATGGTGAGGGCAGCAGCAAAAAATTATAAAAACGTAGCTATTGTAGTCAATAACGATAGTTATAAAAAAATTTTAGAT
GAAATCAATCTTTATGATGGATTACTCAGTTTAGACACTCGTTTTCATTTGGCGGTAAAAGCATTTAAATATGTTACTGA
ATATGATAATACAATTTCTGATTATTTTAATCATCAAGTACAAAGTAACTATCATACGTCCATGACTAATGATCATCATT
GTGATCAATCCTTTTCTTATTTTCCTAAAAATTTAACTTTTATGAATCTTAAATTTATAAAAAAACAAGATATACGTTAC
GGAGAAAACCCACATCAACGCGCAGCATTTTATATGGATATATCTAAGAAACACACAGGATCTATTTCTGATGCTCAGCA
ATTACAAGGAAAACCATTATCTTACAATAATATAGTAGACATGGATACGGCGTTAGAATGCGCGAAAATGTTCGATGAAC
CAACTTGTGTGATTGTTAAACATACCAATCCATGTGGTGTAGCAACATCTGATACTATTTGCACTGCGTATGCTAAAGCT
TATCAAGCAGATCCGATTTCTGCGTTCGGAGGAATTATTGCTTTTAATAGATCTTTAGATAAAAAAACAGCTCAAGCTAT
TATTAGTCAACAATTTGTCGAAGCAATCGTCGCTCCAAATATTAATCGAGATTGTATTAGTATTGTATCTAATAAAAAAC
ATGTACGAATATTGAGGTGTGGAATGTGGACCCCACGAACATCAGATATAGATTTCAAACGAATTAATGGAGGATTATTA
ATACAAGATTATGATGTAATGACAAATTTAGAAGATTTAGAGATAGTTACTATTCGGAAACCAACAAAAGAAGAAATGAA
AGATGCATTATTCTGTTGGAAAGTGGTTAAATTTGTTAAATCTAATGCCATTGTTTGTGGTAAAAATTGTCAAACTATCG
GAATTGGAACCGGCCAAATGAATCGAGTGTATGCGGCAAAAATAGCTACTTCCTTCCAAGAACGTGAACGTCAGGGCACA
TTGAATACTAAAGGATCAGTGATGGCTTCTGATGCTTTTTTTCCTTTTTCTGATATAGTACATATTGCATCTGAAATAGG
TATTAAATGTATTATCCAACCAGGAGGATCTATTCGAGACCAAGAAATTATTAAAACTGCGGATCGGCACGGTATTGCGA
TGATTTTCACACATATTCGTCATTTTAGACATTAA

Upstream 100 bases:

>100_bases
GGGAATGATATATAATAATAATACTATAGGTATGTTACTATTGTGTAATCTTTATTAAGATATATTGATACCGTAGTTTA
ATTATTGTTGTGAGGATATT

Downstream 100 bases:

>100_bases
TTGTTTAAAAAATTATTGTATTTTTCTTGAGAACATACGAGAGTCTTAGACATTTTTTTTTGATAACAAAAGTGATTGTG
TATCAAAAAATAATAGATTT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MIQSSLLIRRVLISVFDKSNILEFAQSLIQRGIQLLSTGGTARILSDAGLPVIKISEYTKLPEIMDGRVKTLHYKVYAGI
LGRRGLDDAIMRQYNIPPIDMVVVNFYSFNSLLAKNKKYSQEEMLDRIDISGPSMVRAAAKNYKNVAIVVNNDSYKKILD
EINLYDGLLSLDTRFHLAVKAFKYVTEYDNTISDYFNHQVQSNYHTSMTNDHHCDQSFSYFPKNLTFMNLKFIKKQDIRY
GENPHQRAAFYMDISKKHTGSISDAQQLQGKPLSYNNIVDMDTALECAKMFDEPTCVIVKHTNPCGVATSDTICTAYAKA
YQADPISAFGGIIAFNRSLDKKTAQAIISQQFVEAIVAPNINRDCISIVSNKKHVRILRCGMWTPRTSDIDFKRINGGLL
IQDYDVMTNLEDLEIVTIRKPTKEEMKDALFCWKVVKFVKSNAIVCGKNCQTIGIGTGQMNRVYAAKIATSFQERERQGT
LNTKGSVMASDAFFPFSDIVHIASEIGIKCIIQPGGSIRDQEIIKTADRHGIAMIFTHIRHFRH

Sequences:

>Translated_544_residues
MIQSSLLIRRVLISVFDKSNILEFAQSLIQRGIQLLSTGGTARILSDAGLPVIKISEYTKLPEIMDGRVKTLHYKVYAGI
LGRRGLDDAIMRQYNIPPIDMVVVNFYSFNSLLAKNKKYSQEEMLDRIDISGPSMVRAAAKNYKNVAIVVNNDSYKKILD
EINLYDGLLSLDTRFHLAVKAFKYVTEYDNTISDYFNHQVQSNYHTSMTNDHHCDQSFSYFPKNLTFMNLKFIKKQDIRY
GENPHQRAAFYMDISKKHTGSISDAQQLQGKPLSYNNIVDMDTALECAKMFDEPTCVIVKHTNPCGVATSDTICTAYAKA
YQADPISAFGGIIAFNRSLDKKTAQAIISQQFVEAIVAPNINRDCISIVSNKKHVRILRCGMWTPRTSDIDFKRINGGLL
IQDYDVMTNLEDLEIVTIRKPTKEEMKDALFCWKVVKFVKSNAIVCGKNCQTIGIGTGQMNRVYAAKIATSFQERERQGT
LNTKGSVMASDAFFPFSDIVHIASEIGIKCIIQPGGSIRDQEIIKTADRHGIAMIFTHIRHFRH
>Mature_544_residues
MIQSSLLIRRVLISVFDKSNILEFAQSLIQRGIQLLSTGGTARILSDAGLPVIKISEYTKLPEIMDGRVKTLHYKVYAGI
LGRRGLDDAIMRQYNIPPIDMVVVNFYSFNSLLAKNKKYSQEEMLDRIDISGPSMVRAAAKNYKNVAIVVNNDSYKKILD
EINLYDGLLSLDTRFHLAVKAFKYVTEYDNTISDYFNHQVQSNYHTSMTNDHHCDQSFSYFPKNLTFMNLKFIKKQDIRY
GENPHQRAAFYMDISKKHTGSISDAQQLQGKPLSYNNIVDMDTALECAKMFDEPTCVIVKHTNPCGVATSDTICTAYAKA
YQADPISAFGGIIAFNRSLDKKTAQAIISQQFVEAIVAPNINRDCISIVSNKKHVRILRCGMWTPRTSDIDFKRINGGLL
IQDYDVMTNLEDLEIVTIRKPTKEEMKDALFCWKVVKFVKSNAIVCGKNCQTIGIGTGQMNRVYAAKIATSFQERERQGT
LNTKGSVMASDAFFPFSDIVHIASEIGIKCIIQPGGSIRDQEIIKTADRHGIAMIFTHIRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=496, Percent_Identity=31.8548387096774, Blast_Score=211, Evalue=1e-54,
Organism=Escherichia coli, GI1790439, Length=544, Percent_Identity=56.6176470588235, Blast_Score=630, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=492, Percent_Identity=27.4390243902439, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI71985574, Length=368, Percent_Identity=22.0108695652174, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71985556, Length=115, Percent_Identity=34.7826086956522, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323768, Length=491, Percent_Identity=29.938900203666, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6323056, Length=514, Percent_Identity=29.3774319066148, Blast_Score=199, Evalue=7e-52,
Organism=Drosophila melanogaster, GI24649832, Length=498, Percent_Identity=31.1244979919679, Blast_Score=196, Evalue=3e-50,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 61434; Mature: 61434

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQSSLLIRRVLISVFDKSNILEFAQSLIQRGIQLLSTGGTARILSDAGLPVIKISEYTK
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEECCHHC
LPEIMDGRVKTLHYKVYAGILGRRGLDDAIMRQYNIPPIDMVVVNFYSFNSLLAKNKKYS
CHHHHCCCCCEEEHEEHHHHHCCCCCCHHHHHHCCCCCHHEEEEEHHHHHHHHHCCCCCC
QEEMLDRIDISGPSMVRAAAKNYKNVAIVVNNDSYKKILDEINLYDGLLSLDTRFHLAVK
HHHHHHHHCCCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHH
AFKYVTEYDNTISDYFNHQVQSNYHTSMTNDHHCDQSFSYFPKNLTFMNLKFIKKQDIRY
HHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCEEEEEEEHHHHCCCC
GENPHQRAAFYMDISKKHTGSISDAQQLQGKPLSYNNIVDMDTALECAKMFDEPTCVIVK
CCCCHHCEEEEEEECCCCCCCCCHHHHHCCCCCCCCCEECHHHHHHHHHHCCCCCEEEEE
HTNPCGVATSDTICTAYAKAYQADPISAFGGIIAFNRSLDKKTAQAIISQQFVEAIVAPN
CCCCCCCCCCCHHHHHHHHHHCCCCHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHCCC
INRDCISIVSNKKHVRILRCGMWTPRTSDIDFKRINGGLLIQDYDVMTNLEDLEIVTIRK
CCHHHHHHHCCCCEEEEEEECCCCCCCCCCCCEECCCCEEEECCHHHCCCCCEEEEEECC
PTKEEMKDALFCWKVVKFVKSNAIVCGKNCQTIGIGTGQMNRVYAAKIATSFQERERQGT
CCHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCC
LNTKGSVMASDAFFPFSDIVHIASEIGIKCIIQPGGSIRDQEIIKTADRHGIAMIFTHIR
CCCCCCEEECCCCCCHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHH
HFRH
HHCC
>Mature Secondary Structure
MIQSSLLIRRVLISVFDKSNILEFAQSLIQRGIQLLSTGGTARILSDAGLPVIKISEYTK
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEECCHHC
LPEIMDGRVKTLHYKVYAGILGRRGLDDAIMRQYNIPPIDMVVVNFYSFNSLLAKNKKYS
CHHHHCCCCCEEEHEEHHHHHCCCCCCHHHHHHCCCCCHHEEEEEHHHHHHHHHCCCCCC
QEEMLDRIDISGPSMVRAAAKNYKNVAIVVNNDSYKKILDEINLYDGLLSLDTRFHLAVK
HHHHHHHHCCCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHH
AFKYVTEYDNTISDYFNHQVQSNYHTSMTNDHHCDQSFSYFPKNLTFMNLKFIKKQDIRY
HHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCEEEEEEEHHHHCCCC
GENPHQRAAFYMDISKKHTGSISDAQQLQGKPLSYNNIVDMDTALECAKMFDEPTCVIVK
CCCCHHCEEEEEEECCCCCCCCCHHHHHCCCCCCCCCEECHHHHHHHHHHCCCCCEEEEE
HTNPCGVATSDTICTAYAKAYQADPISAFGGIIAFNRSLDKKTAQAIISQQFVEAIVAPN
CCCCCCCCCCCHHHHHHHHHHCCCCHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHCCC
INRDCISIVSNKKHVRILRCGMWTPRTSDIDFKRINGGLLIQDYDVMTNLEDLEIVTIRK
CCHHHHHHHCCCCEEEEEEECCCCCCCCCCCCEECCCCEEEECCHHHCCCCCEEEEEECC
PTKEEMKDALFCWKVVKFVKSNAIVCGKNCQTIGIGTGQMNRVYAAKIATSFQERERQGT
CCHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCC
LNTKGSVMASDAFFPFSDIVHIASEIGIKCIIQPGGSIRDQEIIKTADRHGIAMIFTHIR
CCCCCCEEECCCCCCHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHH
HFRH
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA