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The map label for this gene is grpE

Identifier: 71892317

GI number: 71892317

Start: 674875

End: 675462

Strand: Reverse

Name: grpE

Synonym: BPEN_564

Alternate gene names: 71892317

Gene position: 675462-674875 (Counterclockwise)

Preceding gene: 71892320

Following gene: 71892315

Centisome position: 85.32

GC content: 27.89

Gene sequence:

>588_bases
ATGATAGATAATAATGTAAAAAACAATATTGATGAAAGTACATTACAGAATGAAAAAATAGAAAAAGAGGAACTATTAGA
ATCTGTTTCGACAGTAGATAATGTTATTGATCCTAAAAATGATCAAATCATTAAATTAAAGATACAATTAGCACAACTTC
AAGAACATGAACGTAATACGGTATTACGTCTTACAGCTGAAATAGAAAACATCCGTCGGCGTAACACTCAAGAAATAGAA
AAAATCCATAAATTTGGATTAGAACGATTTATCTTTGAATTATTACCAGTCATTGATAATTTAGAACGTACCATGAGTAT
TTCAGATAATTCTAATACTTTATTATCTGCTATAATAGAAGGCATTGAATTAACATTAAAATCGTTTTTAGACACTGTGC
ACAAGTTTGGATTAAAATCTATATATGAAATTAACGTGCCATTTAATCCCGAAATACATCAAGCAATATCTATAATAGAA
TCTGAAGACCATAAACCAAATCAAGTATTAACAATGATTCAAAAAGGATACATTTTAAATGGAAGATTAATTCGACCCGC
TATGGTAACAGTATCACAATCTAAATGA

Upstream 100 bases:

>100_bases
TAACTTTTATTCATAATAAATGATGTCTTGAATACCTGTTATTCATCCTTATAATAACTGAATTATAAAACATATAATCA
GGAAACGGAGGAGAAAATTC

Downstream 100 bases:

>100_bases
CGAACTACTATGTTTTTTAAAATGTTATAAATAAAAAACAGTACTCATTATATATTTACAAAGTTTCATGTACTCTTTCA
TGAAATAATAACGTGTTGTG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MIDNNVKNNIDESTLQNEKIEKEELLESVSTVDNVIDPKNDQIIKLKIQLAQLQEHERNTVLRLTAEIENIRRRNTQEIE
KIHKFGLERFIFELLPVIDNLERTMSISDNSNTLLSAIIEGIELTLKSFLDTVHKFGLKSIYEINVPFNPEIHQAISIIE
SEDHKPNQVLTMIQKGYILNGRLIRPAMVTVSQSK

Sequences:

>Translated_195_residues
MIDNNVKNNIDESTLQNEKIEKEELLESVSTVDNVIDPKNDQIIKLKIQLAQLQEHERNTVLRLTAEIENIRRRNTQEIE
KIHKFGLERFIFELLPVIDNLERTMSISDNSNTLLSAIIEGIELTLKSFLDTVHKFGLKSIYEINVPFNPEIHQAISIIE
SEDHKPNQVLTMIQKGYILNGRLIRPAMVTVSQSK
>Mature_195_residues
MIDNNVKNNIDESTLQNEKIEKEELLESVSTVDNVIDPKNDQIIKLKIQLAQLQEHERNTVLRLTAEIENIRRRNTQEIE
KIHKFGLERFIFELLPVIDNLERTMSISDNSNTLLSAIIEGIELTLKSFLDTVHKFGLKSIYEINVPFNPEIHQAISIIE
SEDHKPNQVLTMIQKGYILNGRLIRPAMVTVSQSK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=149, Percent_Identity=30.8724832214765, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1788967, Length=180, Percent_Identity=52.7777777777778, Blast_Score=198, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6324806, Length=157, Percent_Identity=28.6624203821656, Blast_Score=73, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24653432, Length=136, Percent_Identity=33.8235294117647, Blast_Score=74, Evalue=6e-14,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_BLOPB (Q492C7)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_278051.1
- ProteinModelPortal:   Q492C7
- SMR:   Q492C7
- STRING:   Q492C7
- GeneID:   3563129
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_564
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- ProtClustDB:   PRK14150
- BioCyc:   CBLO291272:BPEN_564-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 22483; Mature: 22483

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDNNVKNNIDESTLQNEKIEKEELLESVSTVDNVIDPKNDQIIKLKIQLAQLQEHERNT
CCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHCHH
VLRLTAEIENIRRRNTQEIEKIHKFGLERFIFELLPVIDNLERTMSISDNSNTLLSAIIE
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHH
GIELTLKSFLDTVHKFGLKSIYEINVPFNPEIHQAISIIESEDHKPNQVLTMIQKGYILN
HHHHHHHHHHHHHHHHCCHHEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCEEEC
GRLIRPAMVTVSQSK
CEEECHHEEEEECCC
>Mature Secondary Structure
MIDNNVKNNIDESTLQNEKIEKEELLESVSTVDNVIDPKNDQIIKLKIQLAQLQEHERNT
CCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHCHH
VLRLTAEIENIRRRNTQEIEKIHKFGLERFIFELLPVIDNLERTMSISDNSNTLLSAIIE
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHH
GIELTLKSFLDTVHKFGLKSIYEINVPFNPEIHQAISIIESEDHKPNQVLTMIQKGYILN
HHHHHHHHHHHHHHHHCCHHEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCEEEC
GRLIRPAMVTVSQSK
CEEECHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA