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The map label for this gene is tadA [H]

Identifier: 71892310

GI number: 71892310

Start: 667443

End: 667910

Strand: Reverse

Name: tadA [H]

Synonym: BPEN_557

Alternate gene names: 71892310

Gene position: 667910-667443 (Counterclockwise)

Preceding gene: 71892311

Following gene: 71892309

Centisome position: 84.37

GC content: 34.83

Gene sequence:

>468_bases
ATGTACGAAATAGAAAATGTAGATACGATATGGATGCGTCATGCTATTGCATTAGCTGCCCATGCTGAAATTATTGGAGA
AATATCAGTAGGAGCTGTTTTAATTCAAAATGGTAAACTTATAAGTTACGGTTGGAATTCTTCTATTATATGCCATGATC
CAAGTGCTCATGCAGAAATTGTAGCGTTACGTACAGGGGGGAAGGTTTTGGGCAATTATCGATTATTAGGTACTACTCTT
TATGTTACTCTAGAACCATGTATGATGTGCATTGGCGCAATAATTCATGCTCGTGTTTATCGATTAGTTTGTGGCGCTAA
AAACAGTAAAACTGAACAAAGATCATGGCTAAAAAATACACTCCGTCATCCAATGAATAATCATCATGTGTTTTTAACAA
CTGGGGTATTAGAAAAAGAATGTGCTTATCAATTGAATAAATTTTTTAAACGTCAACGTAAAACCTGA

Upstream 100 bases:

>100_bases
ATTATTTTGATGTTTTATTGTAATACAGAATGAGTATCTCAATATGCGTTTTAGAATTATTTTATTAATTGTGCATTGTA
ATATTTTTATTACAAGCTGA

Downstream 100 bases:

>100_bases
TATCATCAGAAAATATCAATAAAATTGATCATCTAAAACAACCTTATATCTATTTAAATTTCAAATAAAAACCTATGTGA
GAATATCGTGACAAAAAGAT

Product: tRNA-specific adenosine deaminase

Products: uracil; NH3

Alternate protein names: NA

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MYEIENVDTIWMRHAIALAAHAEIIGEISVGAVLIQNGKLISYGWNSSIICHDPSAHAEIVALRTGGKVLGNYRLLGTTL
YVTLEPCMMCIGAIIHARVYRLVCGAKNSKTEQRSWLKNTLRHPMNNHHVFLTTGVLEKECAYQLNKFFKRQRKT

Sequences:

>Translated_155_residues
MYEIENVDTIWMRHAIALAAHAEIIGEISVGAVLIQNGKLISYGWNSSIICHDPSAHAEIVALRTGGKVLGNYRLLGTTL
YVTLEPCMMCIGAIIHARVYRLVCGAKNSKTEQRSWLKNTLRHPMNNHHVFLTTGVLEKECAYQLNKFFKRQRKT
>Mature_155_residues
MYEIENVDTIWMRHAIALAAHAEIIGEISVGAVLIQNGKLISYGWNSSIICHDPSAHAEIVALRTGGKVLGNYRLLGTTL
YVTLEPCMMCIGAIIHARVYRLVCGAKNSKTEQRSWLKNTLRHPMNNHHVFLTTGVLEKECAYQLNKFFKRQRKT

Specific function: Deaminates adenosine-34 to inosine in tRNA-Arg2 [H]

COG id: COG0590

COG function: function code FJ; Cytosine/adenosine deaminases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytidine and deoxycytidylate deaminase family [H]

Homologues:

Organism=Escherichia coli, GI145693172, Length=154, Percent_Identity=51.9480519480519, Blast_Score=165, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17554608, Length=97, Percent_Identity=38.1443298969072, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17541138, Length=152, Percent_Identity=30.2631578947368, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17555996, Length=97, Percent_Identity=36.0824742268041, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016192
- InterPro:   IPR002125
- InterPro:   IPR016193 [H]

Pfam domain/function: PF00383 dCMP_cyt_deam_1 [H]

EC number: 3.5.4.1

Molecular weight: Translated: 17494; Mature: 17494

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS00903 CYT_DCMP_DEAMINASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEIENVDTIWMRHAIALAAHAEIIGEISVGAVLIQNGKLISYGWNSSIICHDPSAHAEI
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEECCCCEEEEECCCCCEEE
VALRTGGKVLGNYRLLGTTLYVTLEPCMMCIGAIIHARVYRLVCGAKNSKTEQRSWLKNT
EEEECCCEEECCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LRHPMNNHHVFLTTGVLEKECAYQLNKFFKRQRKT
HHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYEIENVDTIWMRHAIALAAHAEIIGEISVGAVLIQNGKLISYGWNSSIICHDPSAHAEI
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEECCCCEEEEECCCCCEEE
VALRTGGKVLGNYRLLGTTLYVTLEPCMMCIGAIIHARVYRLVCGAKNSKTEQRSWLKNT
EEEECCCEEECCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LRHPMNNHHVFLTTGVLEKECAYQLNKFFKRQRKT
HHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: cytosine; H2O

Specific reaction: cytosine + H2O = uracil + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]