Click here to switch to the map view.

The map label for this gene is truA

Identifier: 71892269

GI number: 71892269

Start: 613072

End: 613857

Strand: Reverse

Name: truA

Synonym: BPEN_512

Alternate gene names: 71892269

Gene position: 613857-613072 (Counterclockwise)

Preceding gene: 71892270

Following gene: 71892268

Centisome position: 77.54

GC content: 36.13

Gene sequence:

>786_bases
ATGCAGAAATTAGCTTTAGGCATTGAATACGATGGGTCTTGGTACTGCGGTTGGCAAAGACAAAGAGATGCGCCTAGTAT
CCAGAGTTATATAGAAAATGCTATAAAAAAAATAACATCTGAATCAGTAACGGTATTTTGCGCGGGAAGAACCGATTCTG
GAGTACACGCTTTGGGACAAGTTGTTCATTTTGAAACTTATTCTCGGCGCTCAAAATCAGCTTGGACATTGGGTATGAAT
CGTTATTTGCCGTCAAGTATTTGCGTGCGCTGGGCTAGGTTAGTAAATAAAGACTTTCATGCTCGATTTAGCGCCATTTC
TCGTCGTTATTGTTATATTATTAATAATAATTGCATACGATCTGCTTTGTTATTTAGAAAAACTTGGCATTATAAAAAAT
ATTTAGATATCAATAAAATGTGTGATGCTGCTCAGTATTTATTAGGAGAAAATGATTTCTCTTCGTTTCGTGCAGCAGGG
TGTCAGTCATATTCAGCACGAAGAAAATTATATCATATACGTATAATACGTAAAGGTCAATGTATTGTGATAGATATTAA
AGCTAACTCTTTTATGTATCGTATGGTACGAAATATTGTAGGTAGTTTAGTAAAAGTTGGTTGCGGGGAGCAACCAGAAA
AATGGATTGCAGAGTTGTTAGAAAATTGTAATAGATCACTAGCTGGTGTAACAGCTCCAGCTAGTGGTCTATATTTGGTG
GAAGTTAAATATCCTTCAGATTTTTCTATCCCTTCTTCTTTCATCGAAGAACTGTGGTGCGCATAA

Upstream 100 bases:

>100_bases
ATCAGTTATGTATCTTTATATTTAATTGGAACTCATGTAAACAGCATTAATCTCATATAGATTCATCATAATTAATATAT
TTAATAAAAGTTGTAGTGTA

Downstream 100 bases:

>100_bases
ATATTTTGCATTTTGTATTACATTATTATCACATTTGCACATATTTCTTTCTGTGAATCTGTGTTCTAATGAGAAATGAA
TTACTTGTTTATAATCATTT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MQKLALGIEYDGSWYCGWQRQRDAPSIQSYIENAIKKITSESVTVFCAGRTDSGVHALGQVVHFETYSRRSKSAWTLGMN
RYLPSSICVRWARLVNKDFHARFSAISRRYCYIINNNCIRSALLFRKTWHYKKYLDINKMCDAAQYLLGENDFSSFRAAG
CQSYSARRKLYHIRIIRKGQCIVIDIKANSFMYRMVRNIVGSLVKVGCGEQPEKWIAELLENCNRSLAGVTAPASGLYLV
EVKYPSDFSIPSSFIEELWCA

Sequences:

>Translated_261_residues
MQKLALGIEYDGSWYCGWQRQRDAPSIQSYIENAIKKITSESVTVFCAGRTDSGVHALGQVVHFETYSRRSKSAWTLGMN
RYLPSSICVRWARLVNKDFHARFSAISRRYCYIINNNCIRSALLFRKTWHYKKYLDINKMCDAAQYLLGENDFSSFRAAG
CQSYSARRKLYHIRIIRKGQCIVIDIKANSFMYRMVRNIVGSLVKVGCGEQPEKWIAELLENCNRSLAGVTAPASGLYLV
EVKYPSDFSIPSSFIEELWCA
>Mature_261_residues
MQKLALGIEYDGSWYCGWQRQRDAPSIQSYIENAIKKITSESVTVFCAGRTDSGVHALGQVVHFETYSRRSKSAWTLGMN
RYLPSSICVRWARLVNKDFHARFSAISRRYCYIINNNCIRSALLFRKTWHYKKYLDINKMCDAAQYLLGENDFSSFRAAG
CQSYSARRKLYHIRIIRKGQCIVIDIKANSFMYRMVRNIVGSLVKVGCGEQPEKWIAELLENCNRSLAGVTAPASGLYLV
EVKYPSDFSIPSSFIEELWCA

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=268, Percent_Identity=32.089552238806, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1788657, Length=259, Percent_Identity=55.2123552123552, Blast_Score=301, Evalue=3e-83,
Organism=Drosophila melanogaster, GI116007752, Length=302, Percent_Identity=27.1523178807947, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_BLOPB (Q492H3)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_278003.1
- ProteinModelPortal:   Q492H3
- SMR:   Q492H3
- STRING:   Q492H3
- GeneID:   3563029
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_512
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BioCyc:   CBLO291272:BPEN_512-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29944; Mature: 29944

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: ACT_SITE 52-52 BINDING 110-110 BINDING 237-237

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLALGIEYDGSWYCGWQRQRDAPSIQSYIENAIKKITSESVTVFCAGRTDSGVHALGQ
CCCEEEEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
VVHFETYSRRSKSAWTLGMNRYLPSSICVRWARLVNKDFHARFSAISRRYCYIINNNCIR
HHHHHHHHCCCCCCEEECHHHCCCHHHHHHHHHHHCHHHHHHHHHHCCCEEEEECCHHHH
SALLFRKTWHYKKYLDINKMCDAAQYLLGENDFSSFRAAGCQSYSARRKLYHIRIIRKGQ
HHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHEEEEEEEECCC
CIVIDIKANSFMYRMVRNIVGSLVKVGCGEQPEKWIAELLENCNRSLAGVTAPASGLYLV
EEEEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHCCCCCCCCCEEEE
EVKYPSDFSIPSSFIEELWCA
EEECCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MQKLALGIEYDGSWYCGWQRQRDAPSIQSYIENAIKKITSESVTVFCAGRTDSGVHALGQ
CCCEEEEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
VVHFETYSRRSKSAWTLGMNRYLPSSICVRWARLVNKDFHARFSAISRRYCYIINNNCIR
HHHHHHHHCCCCCCEEECHHHCCCHHHHHHHHHHHCHHHHHHHHHHCCCEEEEECCHHHH
SALLFRKTWHYKKYLDINKMCDAAQYLLGENDFSSFRAAGCQSYSARRKLYHIRIIRKGQ
HHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHEEEEEEEECCC
CIVIDIKANSFMYRMVRNIVGSLVKVGCGEQPEKWIAELLENCNRSLAGVTAPASGLYLV
EEEEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHCCCCCCCCCEEEE
EVKYPSDFSIPSSFIEELWCA
EEECCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA