Click here to switch to the map view.

The map label for this gene is nuoB

Identifier: 71892265

GI number: 71892265

Start: 608675

End: 609385

Strand: Reverse

Name: nuoB

Synonym: BPEN_508

Alternate gene names: 71892265

Gene position: 609385-608675 (Counterclockwise)

Preceding gene: 71892266

Following gene: 71892264

Centisome position: 76.98

GC content: 38.26

Gene sequence:

>711_bases
ATGAAATATACTTTAACGACAGCTCGAACAGATAATAGTGAAGATGAGCAATATCCTCTTCAAGAAAAGAAGATTGTGAC
AGATCCGTTAGAAAAGAATATTCAGCGGAATGTTTTTTTTGGAAAATTAAGTGCTTTTTTACATAAAATTGTTAATTGGG
GGCGAGGCAATTCTTTATGGCCTTATAATTTTGGATTATCCTGTTGTTATGTTGAAATGACTACCGCGTTCACTGCAATA
CATGATGTCGCACGGTTTGGTTCAGAAGTATTACGCGCCTCTCCTCGTCAAGCGGATTTTATGGTTATAGCGGGCACTCC
GTTTCTTAAAATGGTGCCAGTAATACAACGATTATATGATCAAATGTTAGAGCCTAAGTGGGTGATCTCTATGGGGGCTT
GCGCGAATTCTGGAGGTATGTATGATATTTATTCTGTGGTGCAAGGAGTAGATAAATTTCTTCCTGTGGACCTCTATATC
CCTGGATGCCCACCTAGGCCTGAAGCGTATATTCAGGGGTTGTTATTATTAAAAAAGTCTATTAATGAAGAACGACGTCC
ACTTTCTTGGGTGTTGGGAGATCAAGGGATATATAGAAAAAATATGGAGTCAGAGCGTCAACGTAAACGTAATACTCGCA
TAGCAGAAACTGTTTTATCTTCTCCAGATGATGAACTTAATATATATTCAAAACAAAACGGTGAGGCATAA

Upstream 100 bases:

>100_bases
TTGATTGGATAGAACAATCATCGCGACATGTCCATTGTGTAGATTTTTTTGATATTGATACTTCGAGATATTTAAAAAAA
AGTAATAAAAGGTAGCAGAT

Downstream 100 bases:

>100_bases
TCAAGATTTTAAGAATTTTTGTGATAATTGTCATTAGTTGGTAGATATTATGTGTAATGATTCTACAGGTGTTTCTTTAG
TTAAAAATATTTACCCGATA

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MKYTLTTARTDNSEDEQYPLQEKKIVTDPLEKNIQRNVFFGKLSAFLHKIVNWGRGNSLWPYNFGLSCCYVEMTTAFTAI
HDVARFGSEVLRASPRQADFMVIAGTPFLKMVPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDLYI
PGCPPRPEAYIQGLLLLKKSINEERRPLSWVLGDQGIYRKNMESERQRKRNTRIAETVLSSPDDELNIYSKQNGEA

Sequences:

>Translated_236_residues
MKYTLTTARTDNSEDEQYPLQEKKIVTDPLEKNIQRNVFFGKLSAFLHKIVNWGRGNSLWPYNFGLSCCYVEMTTAFTAI
HDVARFGSEVLRASPRQADFMVIAGTPFLKMVPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDLYI
PGCPPRPEAYIQGLLLLKKSINEERRPLSWVLGDQGIYRKNMESERQRKRNTRIAETVLSSPDDELNIYSKQNGEA
>Mature_236_residues
MKYTLTTARTDNSEDEQYPLQEKKIVTDPLEKNIQRNVFFGKLSAFLHKIVNWGRGNSLWPYNFGLSCCYVEMTTAFTAI
HDVARFGSEVLRASPRQADFMVIAGTPFLKMVPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDLYI
PGCPPRPEAYIQGLLLLKKSINEERRPLSWVLGDQGIYRKNMESERQRKRNTRIAETVLSSPDDELNIYSKQNGEA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=147, Percent_Identity=53.7414965986395, Blast_Score=166, Evalue=1e-41,
Organism=Escherichia coli, GI1788624, Length=225, Percent_Identity=74.6666666666667, Blast_Score=359, Evalue=1e-101,
Organism=Escherichia coli, GI1789074, Length=123, Percent_Identity=40.650406504065, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788834, Length=123, Percent_Identity=39.0243902439024, Blast_Score=88, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17509685, Length=147, Percent_Identity=50.3401360544218, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI18859983, Length=147, Percent_Identity=51.0204081632653, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24642371, Length=147, Percent_Identity=51.0204081632653, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24651058, Length=148, Percent_Identity=52.027027027027, Blast_Score=162, Evalue=3e-40,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_BLOPB (Q492H7)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277999.1
- ProteinModelPortal:   Q492H7
- SMR:   Q492H7
- STRING:   Q492H7
- GeneID:   3563025
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_508
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   PRPEAYI
- ProtClustDB:   PRK06411
- BioCyc:   CBLO291272:BPEN_508-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 26957; Mature: 26957

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYTLTTARTDNSEDEQYPLQEKKIVTDPLEKNIQRNVFFGKLSAFLHKIVNWGRGNSLW
CCEEEEECCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PYNFGLSCCYVEMTTAFTAIHDVARFGSEVLRASPRQADFMVIAGTPFLKMVPVIQRLYD
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHH
QMLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDLYIPGCPPRPEAYIQGLLLLKKS
HHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHH
INEERRPLSWVLGDQGIYRKNMESERQRKRNTRIAETVLSSPDDELNIYSKQNGEA
HCHHHCCCEEEECCCCHHHHCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
>Mature Secondary Structure
MKYTLTTARTDNSEDEQYPLQEKKIVTDPLEKNIQRNVFFGKLSAFLHKIVNWGRGNSLW
CCEEEEECCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PYNFGLSCCYVEMTTAFTAIHDVARFGSEVLRASPRQADFMVIAGTPFLKMVPVIQRLYD
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHH
QMLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDLYIPGCPPRPEAYIQGLLLLKKS
HHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHH
INEERRPLSWVLGDQGIYRKNMESERQRKRNTRIAETVLSSPDDELNIYSKQNGEA
HCHHHCCCEEEECCCCHHHHCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA