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The map label for this gene is pykA [H]

Identifier: 71892227

GI number: 71892227

Start: 553587

End: 555035

Strand: Direct

Name: pykA [H]

Synonym: BPEN_465

Alternate gene names: 71892227

Gene position: 553587-555035 (Clockwise)

Preceding gene: 71892223

Following gene: 71892230

Centisome position: 69.93

GC content: 34.37

Gene sequence:

>1449_bases
ATGTCCCAACAATTGAGAAGAACTAAAATTATCGCTACTTTAGGCCCTGCTACAGATAAAGGTAACAATCTTGAAAAAAT
AATTATCTCAGGAGCAAATATCGTACGACTAAATTTTTCTCATGGAAAAACACAAGAACATTTTGTCCGAGCAGAAAAAG
TACGCGAAATCGCTTCCAAACTAAGTACATATATTGCTATTCTTGGTGATTTACAAGGCCCTAAAATCCGTATCTCAACT
TTTCAGGGAAACGAAATACATTTAAAATCTGGAGATAACTTTTTAATTGATACCATGATGACAAACAATAATAAAAGTAA
TCACAAATGCGTAGGTGTTGATTACGAAGAACTCGCACAAGACGTATACCCAGAAGATTTACTTCTATTAGATGATGGGA
GAATTCAATTAAAAGTTATAACAATCCATAATACTAAAATATATACTAAAGTAATTATTGGAGGTACATTATACAACAAT
AAAGGCTTAAACAAATTAGGAGGAGGGCTTTCAGCGAAAGTCTTAACAAACAAGGACAAAATTGATATTATCACCGCGGC
TCAAATTGGAGTTGACTACCTAGCAATATCATTTCCTCGTAGCAGTATAGATTTAAATTGTGCACGAAAATTAATTGCCG
ATGCTGGTAGTCATGCAAAAATTATTTCTAAAATAGAACGCGCAGAAGTAGTTGCATCCGACGAAACAATAGATGACATC
ATTCATGCATCAGACGCAGTTATGGTAGCCCGAGGAGATTTAGGAGTAGAAATTGGAGAACCAGAACTAGTGGGTGTACA
AAAAAAAATAATACAAAGAGCACGCGCTCTTAATCGCGCAGTCATTACTGCAACTCAAATGATGGAATCTATGGTTCATA
ATTCTATGCCTACTCGCGCAGAAGTAATGGACGTAGCCAATGCAGTATTGGATGGTACTGACGCGGTTATGTTATCCGCT
GAAACTGCTACAGGTCAATATCCATCTGAAACTGTAGCAGCTATGGCTAATGTATGTTTGGGTGCCGAAAAAATTTCAAA
CATCAATTTTTTATCAGACATCAATCAAAAAAAAAAAATTGATGATATCGAAGAAGCTATCGCTATATCGGCAATGTACA
CTGCCAATCATCTAGCAGGAGTAAGTGCAATTATCGTTTTGACTGAATTTGAGAGTACTACGTTATTAATGTCTCGTATC
AATTCAAAATTACCTATTTTTGCGCTATCCCAACACAAACACACCTTAAATATAGTTACTCTTTACAAAGGAGTTATTCC
TATTTATTTTAATAGAACAGGGAATAACGTGATAGATGCCAAATATGCTAGTAATTTATTACGTGACAAAGGACTTCTTC
TATCTGGTGAATTAGTAGTTGTGATACAAAATGATATATACAATAAATGTGATGTAATTAATATTAGTCACATTTTACAA
GTAGAATAA

Upstream 100 bases:

>100_bases
AATATAAAATAAACATAAATTATAATACATTAATAACATAATTAATGATCGCCACACTTTAATATTGGTTATTTAAATTT
AAGTTGCATAAGGAACTATT

Downstream 100 bases:

>100_bases
TTATCATGGATTGAAATATTTTTTCCAATTAAATGAAAATTCATAATATCGTTACACATATAATATGTAATATTACTTAA
AAAACCCCGCATGCATGTGC

Product: pyruvate kinase A

Products: NA

Alternate protein names: PK-2 [H]

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MSQQLRRTKIIATLGPATDKGNNLEKIIISGANIVRLNFSHGKTQEHFVRAEKVREIASKLSTYIAILGDLQGPKIRIST
FQGNEIHLKSGDNFLIDTMMTNNNKSNHKCVGVDYEELAQDVYPEDLLLLDDGRIQLKVITIHNTKIYTKVIIGGTLYNN
KGLNKLGGGLSAKVLTNKDKIDIITAAQIGVDYLAISFPRSSIDLNCARKLIADAGSHAKIISKIERAEVVASDETIDDI
IHASDAVMVARGDLGVEIGEPELVGVQKKIIQRARALNRAVITATQMMESMVHNSMPTRAEVMDVANAVLDGTDAVMLSA
ETATGQYPSETVAAMANVCLGAEKISNINFLSDINQKKKIDDIEEAIAISAMYTANHLAGVSAIIVLTEFESTTLLMSRI
NSKLPIFALSQHKHTLNIVTLYKGVIPIYFNRTGNNVIDAKYASNLLRDKGLLLSGELVVVIQNDIYNKCDVINISHILQ
VE

Sequences:

>Translated_482_residues
MSQQLRRTKIIATLGPATDKGNNLEKIIISGANIVRLNFSHGKTQEHFVRAEKVREIASKLSTYIAILGDLQGPKIRIST
FQGNEIHLKSGDNFLIDTMMTNNNKSNHKCVGVDYEELAQDVYPEDLLLLDDGRIQLKVITIHNTKIYTKVIIGGTLYNN
KGLNKLGGGLSAKVLTNKDKIDIITAAQIGVDYLAISFPRSSIDLNCARKLIADAGSHAKIISKIERAEVVASDETIDDI
IHASDAVMVARGDLGVEIGEPELVGVQKKIIQRARALNRAVITATQMMESMVHNSMPTRAEVMDVANAVLDGTDAVMLSA
ETATGQYPSETVAAMANVCLGAEKISNINFLSDINQKKKIDDIEEAIAISAMYTANHLAGVSAIIVLTEFESTTLLMSRI
NSKLPIFALSQHKHTLNIVTLYKGVIPIYFNRTGNNVIDAKYASNLLRDKGLLLSGELVVVIQNDIYNKCDVINISHILQ
VE
>Mature_481_residues
SQQLRRTKIIATLGPATDKGNNLEKIIISGANIVRLNFSHGKTQEHFVRAEKVREIASKLSTYIAILGDLQGPKIRISTF
QGNEIHLKSGDNFLIDTMMTNNNKSNHKCVGVDYEELAQDVYPEDLLLLDDGRIQLKVITIHNTKIYTKVIIGGTLYNNK
GLNKLGGGLSAKVLTNKDKIDIITAAQIGVDYLAISFPRSSIDLNCARKLIADAGSHAKIISKIERAEVVASDETIDDII
HASDAVMVARGDLGVEIGEPELVGVQKKIIQRARALNRAVITATQMMESMVHNSMPTRAEVMDVANAVLDGTDAVMLSAE
TATGQYPSETVAAMANVCLGAEKISNINFLSDINQKKKIDDIEEAIAISAMYTANHLAGVSAIIVLTEFESTTLLMSRIN
SKLPIFALSQHKHTLNIVTLYKGVIPIYFNRTGNNVIDAKYASNLLRDKGLLLSGELVVVIQNDIYNKCDVINISHILQV
E

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=477, Percent_Identity=33.7526205450734, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI10835121, Length=478, Percent_Identity=34.5188284518828, Blast_Score=235, Evalue=7e-62,
Organism=Homo sapiens, GI33286422, Length=477, Percent_Identity=33.3333333333333, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI33286420, Length=477, Percent_Identity=33.3333333333333, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI32967597, Length=478, Percent_Identity=34.5188284518828, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI310128732, Length=292, Percent_Identity=33.5616438356164, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI310128730, Length=292, Percent_Identity=33.5616438356164, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI310128736, Length=238, Percent_Identity=31.5126050420168, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI310128734, Length=238, Percent_Identity=31.5126050420168, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI310128738, Length=217, Percent_Identity=30.4147465437788, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1788160, Length=482, Percent_Identity=63.6929460580913, Blast_Score=626, Evalue=1e-180,
Organism=Escherichia coli, GI1787965, Length=465, Percent_Identity=38.494623655914, Blast_Score=276, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17544584, Length=471, Percent_Identity=35.6687898089172, Blast_Score=265, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI71984413, Length=471, Percent_Identity=35.244161358811, Blast_Score=218, Evalue=7e-57,
Organism=Caenorhabditis elegans, GI17506829, Length=471, Percent_Identity=35.244161358811, Blast_Score=218, Evalue=7e-57,
Organism=Caenorhabditis elegans, GI71984406, Length=471, Percent_Identity=35.244161358811, Blast_Score=217, Evalue=8e-57,
Organism=Caenorhabditis elegans, GI17506831, Length=471, Percent_Identity=35.244161358811, Blast_Score=217, Evalue=8e-57,
Organism=Saccharomyces cerevisiae, GI6324923, Length=472, Percent_Identity=34.5338983050847, Blast_Score=242, Evalue=9e-65,
Organism=Saccharomyces cerevisiae, GI6319279, Length=478, Percent_Identity=33.6820083682008, Blast_Score=227, Evalue=3e-60,
Organism=Drosophila melanogaster, GI28571814, Length=475, Percent_Identity=35.5789473684211, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24648964, Length=475, Percent_Identity=35.5789473684211, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24648966, Length=410, Percent_Identity=36.5853658536585, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24581235, Length=471, Percent_Identity=30.3609341825902, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24646914, Length=276, Percent_Identity=36.231884057971, Blast_Score=144, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52660; Mature: 52528

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQLRRTKIIATLGPATDKGNNLEKIIISGANIVRLNFSHGKTQEHFVRAEKVREIASK
CCCHHHHEEEEEEECCCCCCCCCEEEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHH
LSTYIAILGDLQGPKIRISTFQGNEIHLKSGDNFLIDTMMTNNNKSNHKCVGVDYEELAQ
HHHHHHHEECCCCCEEEEEEECCCEEEEECCCCEEEEEEEECCCCCCCEEECCCHHHHHH
DVYPEDLLLLDDGRIQLKVITIHNTKIYTKVIIGGTLYNNKGLNKLGGGLSAKVLTNKDK
HCCCCCEEEEECCEEEEEEEEEECCEEEEEEEEEEEEECCCCCHHHCCCEEEEEEECCCC
IDIITAAQIGVDYLAISFPRSSIDLNCARKLIADAGSHAKIISKIERAEVVASDETIDDI
EEEEEEHHHCCEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHEECCCHHHHH
IHASDAVMVARGDLGVEIGEPELVGVQKKIIQRARALNRAVITATQMMESMVHNSMPTRA
HHCCCEEEEEECCCCEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
EVMDVANAVLDGTDAVMLSAETATGQYPSETVAAMANVCLGAEKISNINFLSDINQKKKI
HHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCHHHHCCCHHHHHCCHHHCH
DDIEEAIAISAMYTANHLAGVSAIIVLTEFESTTLLMSRINSKLPIFALSQHKHTLNIVT
HHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCHHHHHHHHCCCCCEEEECCCCCEEEEEE
LYKGVIPIYFNRTGNNVIDAKYASNLLRDKGLLLSGELVVVIQNDIYNKCDVINISHILQ
EECCCEEEEEECCCCCEEEHHHHHHHHHCCCCEEECCEEEEEECCCCCCCCEEEEHEEEE
VE
EC
>Mature Secondary Structure 
SQQLRRTKIIATLGPATDKGNNLEKIIISGANIVRLNFSHGKTQEHFVRAEKVREIASK
CCHHHHEEEEEEECCCCCCCCCEEEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHH
LSTYIAILGDLQGPKIRISTFQGNEIHLKSGDNFLIDTMMTNNNKSNHKCVGVDYEELAQ
HHHHHHHEECCCCCEEEEEEECCCEEEEECCCCEEEEEEEECCCCCCCEEECCCHHHHHH
DVYPEDLLLLDDGRIQLKVITIHNTKIYTKVIIGGTLYNNKGLNKLGGGLSAKVLTNKDK
HCCCCCEEEEECCEEEEEEEEEECCEEEEEEEEEEEEECCCCCHHHCCCEEEEEEECCCC
IDIITAAQIGVDYLAISFPRSSIDLNCARKLIADAGSHAKIISKIERAEVVASDETIDDI
EEEEEEHHHCCEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHEECCCHHHHH
IHASDAVMVARGDLGVEIGEPELVGVQKKIIQRARALNRAVITATQMMESMVHNSMPTRA
HHCCCEEEEEECCCCEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
EVMDVANAVLDGTDAVMLSAETATGQYPSETVAAMANVCLGAEKISNINFLSDINQKKKI
HHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCHHHHCCCHHHHHCCHHHCH
DDIEEAIAISAMYTANHLAGVSAIIVLTEFESTTLLMSRINSKLPIFALSQHKHTLNIVT
HHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCHHHHHHHHCCCCCEEEECCCCCEEEEEE
LYKGVIPIYFNRTGNNVIDAKYASNLLRDKGLLLSGELVVVIQNDIYNKCDVINISHILQ
EECCCEEEEEECCCCCEEEHHHHHHHHHCCCCEEECCEEEEEECCCCCCCCEEEEHEEEE
VE
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398 [H]