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The map label for this gene is fabI [H]

Identifier: 71892198

GI number: 71892198

Start: 512610

End: 513398

Strand: Reverse

Name: fabI [H]

Synonym: BPEN_436

Alternate gene names: 71892198

Gene position: 513398-512610 (Counterclockwise)

Preceding gene: 71892207

Following gene: 71892197

Centisome position: 64.85

GC content: 34.47

Gene sequence:

>789_bases
ATGGCATTGCTTAGTAATAAGCGAATTTTAGTTACCGGTGTAGCTAACAATAAATCTATAGCTTACGGTATAGCTCAGGC
CTTACATAGAGAAGGCGCTGAATTAGCTTTTACTTACCATACCGATAAATTAAAATTGAGAGTACAAAATCTGTCTAAAA
ATTTTGATTCTAATATTGTATTACCGTGTGATGTATCCGAAGATTCTTATATAGATAAATTATTTGTTGAGTTGAGAAAA
AAGTGGTCGACTTTCGATGGTTTTATACATGCGATAGCGTTTGCTCCTACTGATCAATTGAAAGGTGATTATGTAGATAC
TGTTACTCGTGAAGGGTTTACATTATCTCATGTTATTAGTTCTTATAGCTTTGTAGGAATAGCTAAAGCATGCGAAAAAA
TGCTTAACAATAGCGCATCCTTAGTTACATTGACTTATTTAGGTGCTATGCGTGCTACTCCTTATTATAATGTAATGGGA
TTAGCTAAGGCTTCATTGGAAGCGAATACTCGTTATATGGCCAATACCATGGGACCAAAAAAAGGTATTCGCGTTAATGC
GATTTCTTGCGGGCCTGTTCGCACTTTGGCGTCCTCTGGTATTAAGAATTTTAAAAAGATGTTGTTGTGTTATCAAAGGC
AATCACCGATGCGTCGTAATATTTCTATTGACGAAATTGGAAATGTTGCTGTATTTTTATGTTCTGATTTATCATCTGGT
ATTACTGGTGAAATAATTTATGTTGATGGAGGATTTAATATCGCATCTAATATAGATGATGTAGAATGA

Upstream 100 bases:

>100_bases
ATGCAATAATATTTGTTACAATTAATTTTAAATGATTGATGTATTGATTATATTTTTTAAATATTGGTTATAAAAAACCT
TATATTTTGGAGATACAAAC

Downstream 100 bases:

>100_bases
TTAATATAAATTAGTAATGCGGGATATAGTTATTATATTTGAATGTTTGAGATCGTTCGTGTTTTTAAAAATTTTTGGTT
ATAGTGAATATGTGATTTCT

Product: enoyl-[acyl-carrier-protein] reductase (NADH)

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MALLSNKRILVTGVANNKSIAYGIAQALHREGAELAFTYHTDKLKLRVQNLSKNFDSNIVLPCDVSEDSYIDKLFVELRK
KWSTFDGFIHAIAFAPTDQLKGDYVDTVTREGFTLSHVISSYSFVGIAKACEKMLNNSASLVTLTYLGAMRATPYYNVMG
LAKASLEANTRYMANTMGPKKGIRVNAISCGPVRTLASSGIKNFKKMLLCYQRQSPMRRNISIDEIGNVAVFLCSDLSSG
ITGEIIYVDGGFNIASNIDDVE

Sequences:

>Translated_262_residues
MALLSNKRILVTGVANNKSIAYGIAQALHREGAELAFTYHTDKLKLRVQNLSKNFDSNIVLPCDVSEDSYIDKLFVELRK
KWSTFDGFIHAIAFAPTDQLKGDYVDTVTREGFTLSHVISSYSFVGIAKACEKMLNNSASLVTLTYLGAMRATPYYNVMG
LAKASLEANTRYMANTMGPKKGIRVNAISCGPVRTLASSGIKNFKKMLLCYQRQSPMRRNISIDEIGNVAVFLCSDLSSG
ITGEIIYVDGGFNIASNIDDVE
>Mature_261_residues
ALLSNKRILVTGVANNKSIAYGIAQALHREGAELAFTYHTDKLKLRVQNLSKNFDSNIVLPCDVSEDSYIDKLFVELRKK
WSTFDGFIHAIAFAPTDQLKGDYVDTVTREGFTLSHVISSYSFVGIAKACEKMLNNSASLVTLTYLGAMRATPYYNVMGL
AKASLEANTRYMANTMGPKKGIRVNAISCGPVRTLASSGIKNFKKMLLCYQRQSPMRRNISIDEIGNVAVFLCSDLSSGI
TGEIIYVDGGFNIASNIDDVE

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=256, Percent_Identity=66.015625, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1789378, Length=256, Percent_Identity=26.953125, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28795; Mature: 28664

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLSNKRILVTGVANNKSIAYGIAQALHREGAELAFTYHTDKLKLRVQNLSKNFDSNIV
CCEECCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCEEEEEEEHHCCCCCCCEE
LPCDVSEDSYIDKLFVELRKKWSTFDGFIHAIAFAPTDQLKGDYVDTVTREGFTLSHVIS
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHCCCCHHHHHHCCCEEHHHHHH
SYSFVGIAKACEKMLNNSASLVTLTYLGAMRATPYYNVMGLAKASLEANTRYMANTMGPK
HHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCHHHHHHHHHHHCCCCCEEHHCCCCC
KGIRVNAISCGPVRTLASSGIKNFKKMLLCYQRQSPMRRNISIDEIGNVAVFLCSDLSSG
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHCCEEEEEECCCCCC
ITGEIIYVDGGFNIASNIDDVE
CCEEEEEEECCCEECCCCCCCC
>Mature Secondary Structure 
ALLSNKRILVTGVANNKSIAYGIAQALHREGAELAFTYHTDKLKLRVQNLSKNFDSNIV
CEECCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCEEEEEEEHHCCCCCCCEE
LPCDVSEDSYIDKLFVELRKKWSTFDGFIHAIAFAPTDQLKGDYVDTVTREGFTLSHVIS
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHCCCCHHHHHHCCCEEHHHHHH
SYSFVGIAKACEKMLNNSASLVTLTYLGAMRATPYYNVMGLAKASLEANTRYMANTMGPK
HHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCHHHHHHHHHHHCCCCCEEHHCCCCC
KGIRVNAISCGPVRTLASSGIKNFKKMLLCYQRQSPMRRNISIDEIGNVAVFLCSDLSSG
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHCCEEEEEECCCCCC
ITGEIIYVDGGFNIASNIDDVE
CCEEEEEEECCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]