Click here to switch to the map view.

The map label for this gene is purB [H]

Identifier: 71892172

GI number: 71892172

Start: 480986

End: 482368

Strand: Direct

Name: purB [H]

Synonym: BPEN_405

Alternate gene names: 71892172

Gene position: 480986-482368 (Clockwise)

Preceding gene: 71892171

Following gene: 71892189

Centisome position: 60.76

GC content: 30.15

Gene sequence:

>1383_bases
GTGATAACATTATCGCCCTTAACTGTAATATCTCCTATTGATGGCCGTTACTACAAACAAACTGATTCTTTACGTAATAT
TTTTAGTGAATTTAGTTTTCTGAAATTTCGTGTACAAATAGAGATTTATTGGTTGAAAAAACTAGCTAACACAATTGCTA
TTACGGAAATACCACCTTTTACACAAATCGAATCTGATTTTTTAAATAAACTGACAATCAACTTTAATATTAAAGACGCA
GAACGTATTAAAGAAATAGAAAAAATTACCAATCATGATCTCAAAGCAATAGAATATTTTTTAAAAGAAAAAATAAACAT
ATTACCTAATTTAAAAAAAATCAGTGAATTTATTCATTTTGCTTGTACTTCAGACGATATTAACAATCTTGCGTATGGAT
TGATGCTGACTAACACTAAAAAAACTGTTTTTTTGCCCATTTGGAAACAAATTATTAATGAAATAAAAAAAATGAGTATT
AATTATAAAGATATTCCTGTGTTATCTAGAACCCATGGACAACCCGCTACCCCTTCTACTATCGGAAAGGAAATGGCTAA
TATTGCATACCGTTTGATACGTCAATATCATCAATTAAAATCAATTAAGATTTTAGGTAAAATGAATGGTGCCGTAGGAA
ACTATAATGCTCACGTAATAGCATATCCAGAAATAAATTGGCGCAAATTCAGTGAAGAGTTTGTTATATCTTTAGGCATG
AAATGGAACCCTTATACAACACAAATAGAACCACACGATTATATTGCCGAACTATCCGACTGCATTGCACGATTTAATAC
CATATTAATAAATTTTAATAGAGATATATGGGGATATATTGCTCTTAATTATTTTAAACAAAATCATAGCAATACTACAA
TCGGATCTTCTACTATGCCATATAAAGTAAATCCTATAAACTTTGAAAATTCTGAAGGAAATTTAGGGTTAGCAAACGCC
ATGTTTAACCATTTTTCCGCTAAACTTCCAATATCGCGTTGGCAACGTGATTTAAGCGATTCTACTGTTTTAAGAAATCT
AGGTATGGCCATGGGATATGCCTTAATTGCCTACCATAGCATATTAAAAGGTATAAAAACACTGACAGTTAATAAAGAAC
ATGTATTAACCGAGTTAAATAAAAACTGGATAGTGTTAAGCGAAGCTATTCAAACAGTAATGCGACGACACAATATTCAT
GAATCTTATGAATGCACAAAACAATTCATCAATAATACAGAAATTAATCCTGAAAAAATAAAAATTTTTATTGAATCACT
GCCATTACCAGCAACAGAAAAAATACGATTACAAAAAATCACACCACTTGATTACATAGGATTGGCGGCAGTCATGGCAA
ATGATATCACTGATATAATATAA

Upstream 100 bases:

>100_bases
AAAATATTCAATAAGATACACATATTTTATCTTGGATAAACTTGTAATTTTTAAATATCATATAAAATATGAGCATCTAG
TTAATTATGAAGAGGAAAAA

Downstream 100 bases:

>100_bases
TAATAAAACTATAAATAAATTTCTATAAAGCACATGAAATGGACCGAACGATTCACGTTATATTGTATTTATAATACATA
ACAATCACATTCATTTTGAA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MITLSPLTVISPIDGRYYKQTDSLRNIFSEFSFLKFRVQIEIYWLKKLANTIAITEIPPFTQIESDFLNKLTINFNIKDA
ERIKEIEKITNHDLKAIEYFLKEKINILPNLKKISEFIHFACTSDDINNLAYGLMLTNTKKTVFLPIWKQIINEIKKMSI
NYKDIPVLSRTHGQPATPSTIGKEMANIAYRLIRQYHQLKSIKILGKMNGAVGNYNAHVIAYPEINWRKFSEEFVISLGM
KWNPYTTQIEPHDYIAELSDCIARFNTILINFNRDIWGYIALNYFKQNHSNTTIGSSTMPYKVNPINFENSEGNLGLANA
MFNHFSAKLPISRWQRDLSDSTVLRNLGMAMGYALIAYHSILKGIKTLTVNKEHVLTELNKNWIVLSEAIQTVMRRHNIH
ESYECTKQFINNTEINPEKIKIFIESLPLPATEKIRLQKITPLDYIGLAAVMANDITDII

Sequences:

>Translated_460_residues
MITLSPLTVISPIDGRYYKQTDSLRNIFSEFSFLKFRVQIEIYWLKKLANTIAITEIPPFTQIESDFLNKLTINFNIKDA
ERIKEIEKITNHDLKAIEYFLKEKINILPNLKKISEFIHFACTSDDINNLAYGLMLTNTKKTVFLPIWKQIINEIKKMSI
NYKDIPVLSRTHGQPATPSTIGKEMANIAYRLIRQYHQLKSIKILGKMNGAVGNYNAHVIAYPEINWRKFSEEFVISLGM
KWNPYTTQIEPHDYIAELSDCIARFNTILINFNRDIWGYIALNYFKQNHSNTTIGSSTMPYKVNPINFENSEGNLGLANA
MFNHFSAKLPISRWQRDLSDSTVLRNLGMAMGYALIAYHSILKGIKTLTVNKEHVLTELNKNWIVLSEAIQTVMRRHNIH
ESYECTKQFINNTEINPEKIKIFIESLPLPATEKIRLQKITPLDYIGLAAVMANDITDII
>Mature_460_residues
MITLSPLTVISPIDGRYYKQTDSLRNIFSEFSFLKFRVQIEIYWLKKLANTIAITEIPPFTQIESDFLNKLTINFNIKDA
ERIKEIEKITNHDLKAIEYFLKEKINILPNLKKISEFIHFACTSDDINNLAYGLMLTNTKKTVFLPIWKQIINEIKKMSI
NYKDIPVLSRTHGQPATPSTIGKEMANIAYRLIRQYHQLKSIKILGKMNGAVGNYNAHVIAYPEINWRKFSEEFVISLGM
KWNPYTTQIEPHDYIAELSDCIARFNTILINFNRDIWGYIALNYFKQNHSNTTIGSSTMPYKVNPINFENSEGNLGLANA
MFNHFSAKLPISRWQRDLSDSTVLRNLGMAMGYALIAYHSILKGIKTLTVNKEHVLTELNKNWIVLSEAIQTVMRRHNIH
ESYECTKQFINNTEINPEKIKIFIESLPLPATEKIRLQKITPLDYIGLAAVMANDITDII

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=455, Percent_Identity=61.3186813186813, Blast_Score=625, Evalue=1e-180,
Organism=Saccharomyces cerevisiae, GI6323391, Length=349, Percent_Identity=25.214899713467, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 53105; Mature: 53105

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLSPLTVISPIDGRYYKQTDSLRNIFSEFSFLKFRVQIEIYWLKKLANTIAITEIPPF
CEEECCEEEECCCCCCHHHHHHHHHHHHHHHHEEEEEEEEEEEEHHHHHCEEEEECCCCC
TQIESDFLNKLTINFNIKDAERIKEIEKITNHDLKAIEYFLKEKINILPNLKKISEFIHF
HHHHHHHHCEEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ACTSDDINNLAYGLMLTNTKKTVFLPIWKQIINEIKKMSINYKDIPVLSRTHGQPATPST
HCCCCCHHHHEEEEEEECCCCEEEHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCHHH
IGKEMANIAYRLIRQYHQLKSIKILGKMNGAVGNYNAHVIAYPEINWRKFSEEFVISLGM
HHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEEEEECCCCHHHHCCHHHEEECC
KWNPYTTQIEPHDYIAELSDCIARFNTILINFNRDIWGYIALNYFKQNHSNTTIGSSTMP
CCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCEEEHHHHHHHHHCCCCCCCCCCCCC
YKVNPINFENSEGNLGLANAMFNHFSAKLPISRWQRDLSDSTVLRNLGMAMGYALIAYHS
EEECCCCCCCCCCCEEHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ILKGIKTLTVNKEHVLTELNKNWIVLSEAIQTVMRRHNIHESYECTKQFINNTEINPEKI
HHHHHHEEECCHHHHHHHCCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHE
KIFIESLPLPATEKIRLQKITPLDYIGLAAVMANDITDII
EEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MITLSPLTVISPIDGRYYKQTDSLRNIFSEFSFLKFRVQIEIYWLKKLANTIAITEIPPF
CEEECCEEEECCCCCCHHHHHHHHHHHHHHHHEEEEEEEEEEEEHHHHHCEEEEECCCCC
TQIESDFLNKLTINFNIKDAERIKEIEKITNHDLKAIEYFLKEKINILPNLKKISEFIHF
HHHHHHHHCEEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ACTSDDINNLAYGLMLTNTKKTVFLPIWKQIINEIKKMSINYKDIPVLSRTHGQPATPST
HCCCCCHHHHEEEEEEECCCCEEEHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCHHH
IGKEMANIAYRLIRQYHQLKSIKILGKMNGAVGNYNAHVIAYPEINWRKFSEEFVISLGM
HHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEEEEECCCCHHHHCCHHHEEECC
KWNPYTTQIEPHDYIAELSDCIARFNTILINFNRDIWGYIALNYFKQNHSNTTIGSSTMP
CCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCEEEHHHHHHHHHCCCCCCCCCCCCC
YKVNPINFENSEGNLGLANAMFNHFSAKLPISRWQRDLSDSTVLRNLGMAMGYALIAYHS
EEECCCCCCCCCCCEEHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ILKGIKTLTVNKEHVLTELNKNWIVLSEAIQTVMRRHNIHESYECTKQFINNTEINPEKI
HHHHHHEEECCHHHHHHHCCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHE
KIFIESLPLPATEKIRLQKITPLDYIGLAAVMANDITDII
EEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]