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The map label for this gene is lplA

Identifier: 71892136

GI number: 71892136

Start: 435873

End: 436892

Strand: Direct

Name: lplA

Synonym: BPEN_368

Alternate gene names: 71892136

Gene position: 435873-436892 (Clockwise)

Preceding gene: 71892135

Following gene: 71892137

Centisome position: 55.06

GC content: 33.53

Gene sequence:

>1020_bases
ATGTGTTCTTTGCGGTTATTATTATCTGACTCTTACGATCCCTGGTTTAATTTGTCACTTGAAGAATATATTTATAAAAA
TATACCTAAAAAGCAATCTATACTGTTTTTGTGGAGAAATCAAAATACAGTAGTTATAGGACGTGCTCAAAATGCATGGA
AAGAATGTAATACTCGCCGTATGGAACGAGATGGAATTAAATTAGCTAGAAGAAATAGCGGAGGCGGCGCTGTGTTTCAT
GATTTAGGCAACACTTGTTTTACTTTTATTTCTACTCAAGAGCATTATGATAAAAGCATATCCTCTAATATAGTTTTGAA
TGGATTGAGTTATATTGGGATTAAGGCTATCATTTCTGGACGAAATGACATTGTCATACGTACAGAAAATGGAGAACATC
GTAAAATTAGCGGATCTGCATATCGTGAAACATCTGGACGTAAATTTCATCACGGCACATTACTTTTACACGTTGACATT
GATAAACTTGATTATTATCTTAATCCAGATTTTAAAAAACTAGAGACTAAAGGGATTACCTCTATTCGATCAAGAGTTGC
TAATTTAAATGAATTAAAACCTGGTATTAATCACCAAGAAGTATGTCTAGGATTAACAGAAGCATTTTTCCAACATTATG
GAATGAAAGTAAAACCAGAAATATTATCTACAGATAATTTCTGCAAAATTCCAGAATTTTTTAAACAATTCAACAAACAA
AGTGACTGGAACTGGAATTTTGGCAGCGCTCCTGCATTTACGCATCAATTGGATACCCGTTTTGATTGGGGCAGTGTAAC
GTTACATTGCGACATAGAACGTGGAATCATTCATCGTAGCCACATTTTCACTGACAGTTTAGACCCGAGTCCTTTAGAAA
TATTAGCAAAAAAATTAGTAGGTATCCCTTATAACAATAAAAGTATCCTATGTTGCTGTAAAGAATGGATGCAAAGCTGG
CCTCAATACAAAAAAGAATTATCAGAGGTTTCTCATTGGTTAATCAAAACAATATCCTAA

Upstream 100 bases:

>100_bases
ATAAAAAATAATTAAAAAATGATTATTATTAATGATAATATAATTATATTTTACATATAAGATTTACCTTTCGATATTAA
ATATCGAAAAAGGAGGAACT

Downstream 100 bases:

>100_bases
TTCACTGTTAGCTTTTACTGGGCCCTAATTTTTATTAATTTTTGAGATTTTTAACATAATTACATACATGTAAATTATGC
ATATTGATTACAACTAGATT

Product: lipoate-protein ligase A

Products: NA

Alternate protein names: Lipoate--protein ligase

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MCSLRLLLSDSYDPWFNLSLEEYIYKNIPKKQSILFLWRNQNTVVIGRAQNAWKECNTRRMERDGIKLARRNSGGGAVFH
DLGNTCFTFISTQEHYDKSISSNIVLNGLSYIGIKAIISGRNDIVIRTENGEHRKISGSAYRETSGRKFHHGTLLLHVDI
DKLDYYLNPDFKKLETKGITSIRSRVANLNELKPGINHQEVCLGLTEAFFQHYGMKVKPEILSTDNFCKIPEFFKQFNKQ
SDWNWNFGSAPAFTHQLDTRFDWGSVTLHCDIERGIIHRSHIFTDSLDPSPLEILAKKLVGIPYNNKSILCCCKEWMQSW
PQYKKELSEVSHWLIKTIS

Sequences:

>Translated_339_residues
MCSLRLLLSDSYDPWFNLSLEEYIYKNIPKKQSILFLWRNQNTVVIGRAQNAWKECNTRRMERDGIKLARRNSGGGAVFH
DLGNTCFTFISTQEHYDKSISSNIVLNGLSYIGIKAIISGRNDIVIRTENGEHRKISGSAYRETSGRKFHHGTLLLHVDI
DKLDYYLNPDFKKLETKGITSIRSRVANLNELKPGINHQEVCLGLTEAFFQHYGMKVKPEILSTDNFCKIPEFFKQFNKQ
SDWNWNFGSAPAFTHQLDTRFDWGSVTLHCDIERGIIHRSHIFTDSLDPSPLEILAKKLVGIPYNNKSILCCCKEWMQSW
PQYKKELSEVSHWLIKTIS
>Mature_339_residues
MCSLRLLLSDSYDPWFNLSLEEYIYKNIPKKQSILFLWRNQNTVVIGRAQNAWKECNTRRMERDGIKLARRNSGGGAVFH
DLGNTCFTFISTQEHYDKSISSNIVLNGLSYIGIKAIISGRNDIVIRTENGEHRKISGSAYRETSGRKFHHGTLLLHVDI
DKLDYYLNPDFKKLETKGITSIRSRVANLNELKPGINHQEVCLGLTEAFFQHYGMKVKPEILSTDNFCKIPEFFKQFNKQ
SDWNWNFGSAPAFTHQLDTRFDWGSVTLHCDIERGIIHRSHIFTDSLDPSPLEILAKKLVGIPYNNKSILCCCKEWMQSW
PQYKKELSEVSHWLIKTIS

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family

Homologues:

Organism=Homo sapiens, GI7706252, Length=259, Percent_Identity=30.5019305019305, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI21729884, Length=259, Percent_Identity=30.5019305019305, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI21729882, Length=259, Percent_Identity=30.5019305019305, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI21729880, Length=259, Percent_Identity=30.5019305019305, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI21729878, Length=259, Percent_Identity=30.5019305019305, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1790846, Length=338, Percent_Identity=58.5798816568047, Blast_Score=434, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI71981909, Length=271, Percent_Identity=29.520295202952, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6322415, Length=284, Percent_Identity=26.7605633802817, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI28573464, Length=330, Percent_Identity=32.1212121212121, Blast_Score=131, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LPLA_BLOPB (Q492U9)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277868.1
- HSSP:   P32099
- ProteinModelPortal:   Q492U9
- SMR:   Q492U9
- STRING:   Q492U9
- GeneID:   3563042
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_368
- eggNOG:   COG0095
- HOGENOM:   HBG715377
- OMA:   YEQSHLE
- ProtClustDB:   CLSK280831
- BioCyc:   CBLO291272:BPEN_368-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01602
- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562
- Gene3D:   G3DSA:3.30.390.50
- TIGRFAMs:   TIGR00545

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C

EC number: =2.7.7.63

Molecular weight: Translated: 39198; Mature: 39198

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: BINDING 71-71 BINDING 135-135 BINDING 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSLRLLLSDSYDPWFNLSLEEYIYKNIPKKQSILFLWRNQNTVVIGRAQNAWKECNTRR
CCEEEEEECCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCEEEEECCHHHHHHHHHHH
MERDGIKLARRNSGGGAVFHDLGNTCFTFISTQEHYDKSISSNIVLNGLSYIGIKAIISG
HHHCCCEEEEECCCCCEEHHHCCCHHEEEECCHHHHHHHHCCCEEECCHHHHEEEEEECC
RNDIVIRTENGEHRKISGSAYRETSGRKFHHGTLLLHVDIDKLDYYLNPDFKKLETKGIT
CCCEEEEECCCCCEEECCCHHHCCCCCEEECCEEEEEEEHHHEEEEECCCHHHHHHHHHH
SIRSRVANLNELKPGINHQEVCLGLTEAFFQHYGMKVKPEILSTDNFCKIPEFFKQFNKQ
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEECCHHCCCCCCCCCHHHHHHHCCC
SDWNWNFGSAPAFTHQLDTRFDWGSVTLHCDIERGIIHRSHIFTDSLDPSPLEILAKKLV
CCCCCCCCCCCCHHHHCCCCCCCCCEEEEEECCCCEEEHHHEECCCCCCCHHHHHHHHHH
GIPYNNKSILCCCKEWMQSWPQYKKELSEVSHWLIKTIS
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MCSLRLLLSDSYDPWFNLSLEEYIYKNIPKKQSILFLWRNQNTVVIGRAQNAWKECNTRR
CCEEEEEECCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCEEEEECCHHHHHHHHHHH
MERDGIKLARRNSGGGAVFHDLGNTCFTFISTQEHYDKSISSNIVLNGLSYIGIKAIISG
HHHCCCEEEEECCCCCEEHHHCCCHHEEEECCHHHHHHHHCCCEEECCHHHHEEEEEECC
RNDIVIRTENGEHRKISGSAYRETSGRKFHHGTLLLHVDIDKLDYYLNPDFKKLETKGIT
CCCEEEEECCCCCEEECCCHHHCCCCCEEECCEEEEEEEHHHEEEEECCCHHHHHHHHHH
SIRSRVANLNELKPGINHQEVCLGLTEAFFQHYGMKVKPEILSTDNFCKIPEFFKQFNKQ
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEECCHHCCCCCCCCCHHHHHHHCCC
SDWNWNFGSAPAFTHQLDTRFDWGSVTLHCDIERGIIHRSHIFTDSLDPSPLEILAKKLV
CCCCCCCCCCCCHHHHCCCCCCCCCEEEEEECCCCEEEHHHEECCCCCCCHHHHHHHHHH
GIPYNNKSILCCCKEWMQSWPQYKKELSEVSHWLIKTIS
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA