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The map label for this gene is metK

Identifier: 71892033

GI number: 71892033

Start: 293740

End: 294909

Strand: Reverse

Name: metK

Synonym: BPEN_259

Alternate gene names: 71892033

Gene position: 294909-293740 (Counterclockwise)

Preceding gene: 71892038

Following gene: 71892032

Centisome position: 37.25

GC content: 36.5

Gene sequence:

>1170_bases
ATGTCGCAATATTCGTTTACATCTGAGTCCGTATCAGAAGGACATCCGGATAAAATTGCAGATCAGATTTCTGATGCTAT
ATTGGATTCTATCTTAGCGCAGGATCCAAGAGCTCGTGTTGCGTGTGAAACTTATGTTAAGACAGGTATAGTAATAATGG
GCGGGGAAATTACTACTACTGCTTGGGTAAATATGGAAGAAATCGCTAGAAACACTATTCGTGATATTGGTTATGTTCAT
TCAGATATGGGGTTTGATGCAAATTCTTGCGTAATATTGAGTGTAATTAATAAACAATCTTCAGATATTCAATATAGTAT
TGATCATGGTAATACAAGTACATTACAACGAGGAGCAGGTGATCAGGGATTAATGTTTGGATATGCTACTAATGAAACTA
GTGTTTTCATGCCTGCTCCGATAACATATGCGCATCGTTTGATTAAAAGAAAATCTCAAGTGAGAAAAAGTGGAGTGTTG
CCTTGGTTGGGATTAGATGCTAAAAGTCAAGTAACTGTTGCTTATGAGCATGGCAAGATCATTGGCATCACTGCTGTTGT
CTTGTCTATTCAACATGCTTGTGATATAAAGTTAACAGATTTGAAAGAAGCTGCTATGGAAGAAATTATTAAACCAGTGT
TACCTAACAAATGGTTAACTAATAACACTATATTTCTTATTAATCCTGGCGGGCGTTTTGTTATCGGAGGTCCTATTAGT
GATTGTGGTTTAACTGGAAGAAAAATCATTGTAGATACCTATGGAGGTATAGGTCGGCATGGGGGCGGATCTTTTTCTGG
AAAAGATCCTTCAAAAGTAGATCGTTCGGCTGCTTACGGAGCAAGATATGTAGCTAAAAATATTGTAGCGGCTGGATTAG
CAGATCGCTGCGAGCTGCAAGTATCCTATGCAATTGGTGTTCCACATCCAATTTCTATGAGTATTGAAACTTTTGGAACA
GAAAAAATATCACATGACATTTTAATGCAATTAATAAAAAATTTTTTTGATTTTCGTCCTTATGGTTTAATTGCCATGTT
AGATTTATTAAGACCAATTTACAAAGAAACAGCTGTTTATGGACATTTTGGTCGTGAATGTTTCCCTTGGGAAAAAATTG
ATAAAGCAGACCTTCTTCGTGATGCGGCTGGTTTGAAATGTACACCTTAA

Upstream 100 bases:

>100_bases
AATAATTTTATGCGTATGATTAATTCAAATAAATTTACTATACCTATGTTGGGTCATACTAATGTAAGTCACGAACTTGA
TACTAAAAAGGATATAAAAA

Downstream 100 bases:

>100_bases
TAGTATTCACATACTTATTTTTAAGTTGATTTGTAATGATTTTATTAATATGGGAATATACAATGTGTTGATCAATGATC
ATACAATAAGAAGATTGATA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MSQYSFTSESVSEGHPDKIADQISDAILDSILAQDPRARVACETYVKTGIVIMGGEITTTAWVNMEEIARNTIRDIGYVH
SDMGFDANSCVILSVINKQSSDIQYSIDHGNTSTLQRGAGDQGLMFGYATNETSVFMPAPITYAHRLIKRKSQVRKSGVL
PWLGLDAKSQVTVAYEHGKIIGITAVVLSIQHACDIKLTDLKEAAMEEIIKPVLPNKWLTNNTIFLINPGGRFVIGGPIS
DCGLTGRKIIVDTYGGIGRHGGGSFSGKDPSKVDRSAAYGARYVAKNIVAAGLADRCELQVSYAIGVPHPISMSIETFGT
EKISHDILMQLIKNFFDFRPYGLIAMLDLLRPIYKETAVYGHFGRECFPWEKIDKADLLRDAAGLKCTP

Sequences:

>Translated_389_residues
MSQYSFTSESVSEGHPDKIADQISDAILDSILAQDPRARVACETYVKTGIVIMGGEITTTAWVNMEEIARNTIRDIGYVH
SDMGFDANSCVILSVINKQSSDIQYSIDHGNTSTLQRGAGDQGLMFGYATNETSVFMPAPITYAHRLIKRKSQVRKSGVL
PWLGLDAKSQVTVAYEHGKIIGITAVVLSIQHACDIKLTDLKEAAMEEIIKPVLPNKWLTNNTIFLINPGGRFVIGGPIS
DCGLTGRKIIVDTYGGIGRHGGGSFSGKDPSKVDRSAAYGARYVAKNIVAAGLADRCELQVSYAIGVPHPISMSIETFGT
EKISHDILMQLIKNFFDFRPYGLIAMLDLLRPIYKETAVYGHFGRECFPWEKIDKADLLRDAAGLKCTP
>Mature_388_residues
SQYSFTSESVSEGHPDKIADQISDAILDSILAQDPRARVACETYVKTGIVIMGGEITTTAWVNMEEIARNTIRDIGYVHS
DMGFDANSCVILSVINKQSSDIQYSIDHGNTSTLQRGAGDQGLMFGYATNETSVFMPAPITYAHRLIKRKSQVRKSGVLP
WLGLDAKSQVTVAYEHGKIIGITAVVLSIQHACDIKLTDLKEAAMEEIIKPVLPNKWLTNNTIFLINPGGRFVIGGPISD
CGLTGRKIIVDTYGGIGRHGGGSFSGKDPSKVDRSAAYGARYVAKNIVAAGLADRCELQVSYAIGVPHPISMSIETFGTE
KISHDILMQLIKNFFDFRPYGLIAMLDLLRPIYKETAVYGHFGRECFPWEKIDKADLLRDAAGLKCTP

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=372, Percent_Identity=54.0322580645161, Blast_Score=384, Evalue=1e-107,
Organism=Homo sapiens, GI5174529, Length=372, Percent_Identity=52.9569892473118, Blast_Score=383, Evalue=1e-106,
Organism=Escherichia coli, GI1789311, Length=386, Percent_Identity=74.3523316062176, Blast_Score=611, Evalue=1e-176,
Organism=Caenorhabditis elegans, GI17538498, Length=390, Percent_Identity=51.025641025641, Blast_Score=378, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI25145633, Length=384, Percent_Identity=50.78125, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17538494, Length=378, Percent_Identity=51.5873015873016, Blast_Score=373, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17551082, Length=375, Percent_Identity=51.2, Blast_Score=373, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI32565905, Length=378, Percent_Identity=46.8253968253968, Blast_Score=315, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI32565911, Length=343, Percent_Identity=47.8134110787172, Blast_Score=305, Evalue=4e-83,
Organism=Caenorhabditis elegans, GI25145635, Length=336, Percent_Identity=47.6190476190476, Blast_Score=300, Evalue=7e-82,
Organism=Caenorhabditis elegans, GI32565903, Length=300, Percent_Identity=48.6666666666667, Blast_Score=271, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI32565909, Length=87, Percent_Identity=59.7701149425287, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI32565907, Length=50, Percent_Identity=68, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320710, Length=379, Percent_Identity=52.2427440633245, Blast_Score=372, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6323209, Length=373, Percent_Identity=52.5469168900804, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI45552159, Length=373, Percent_Identity=50.6702412868633, Blast_Score=350, Evalue=9e-97,
Organism=Drosophila melanogaster, GI24580529, Length=373, Percent_Identity=50.6702412868633, Blast_Score=350, Evalue=9e-97,
Organism=Drosophila melanogaster, GI24580537, Length=373, Percent_Identity=50.6702412868633, Blast_Score=350, Evalue=9e-97,
Organism=Drosophila melanogaster, GI24580533, Length=373, Percent_Identity=50.6702412868633, Blast_Score=350, Evalue=9e-97,
Organism=Drosophila melanogaster, GI24580535, Length=373, Percent_Identity=50.6702412868633, Blast_Score=350, Evalue=9e-97,
Organism=Drosophila melanogaster, GI24580531, Length=373, Percent_Identity=50.6702412868633, Blast_Score=350, Evalue=9e-97,
Organism=Drosophila melanogaster, GI24580527, Length=373, Percent_Identity=50.6702412868633, Blast_Score=344, Evalue=5e-95,
Organism=Drosophila melanogaster, GI24580525, Length=373, Percent_Identity=50.6702412868633, Blast_Score=344, Evalue=5e-95,
Organism=Drosophila melanogaster, GI24580541, Length=360, Percent_Identity=49.7222222222222, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=51.2605042016807, Blast_Score=118, Evalue=6e-27,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_BLOPB (Q493F2)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277763.1
- ProteinModelPortal:   Q493F2
- SMR:   Q493F2
- STRING:   Q493F2
- GeneID:   3562925
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_259
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- BioCyc:   CBLO291272:BPEN_259-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42512; Mature: 42381

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQYSFTSESVSEGHPDKIADQISDAILDSILAQDPRARVACETYVKTGIVIMGGEITTT
CCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCEEEEE
AWVNMEEIARNTIRDIGYVHSDMGFDANSCVILSVINKQSSDIQYSIDHGNTSTLQRGAG
EECCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEECCCCHHHHHCCCC
DQGLMFGYATNETSVFMPAPITYAHRLIKRKSQVRKSGVLPWLGLDAKSQVTVAYEHGKI
CCCEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEEEECCCE
IGITAVVLSIQHACDIKLTDLKEAAMEEIIKPVLPNKWLTNNTIFLINPGGRFVIGGPIS
EEHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHCCCCEECCCEEEEECCCCCEEECCCCC
DCGLTGRKIIVDTYGGIGRHGGGSFSGKDPSKVDRSAAYGARYVAKNIVAAGLADRCELQ
CCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
VSYAIGVPHPISMSIETFGTEKISHDILMQLIKNFFDFRPYGLIAMLDLLRPIYKETAVY
EEEEECCCCCCEEEHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GHFGRECFPWEKIDKADLLRDAAGLKCTP
HCCCCCCCCHHHCCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SQYSFTSESVSEGHPDKIADQISDAILDSILAQDPRARVACETYVKTGIVIMGGEITTT
CCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCEEEEE
AWVNMEEIARNTIRDIGYVHSDMGFDANSCVILSVINKQSSDIQYSIDHGNTSTLQRGAG
EECCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEECCCCHHHHHCCCC
DQGLMFGYATNETSVFMPAPITYAHRLIKRKSQVRKSGVLPWLGLDAKSQVTVAYEHGKI
CCCEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEEEECCCE
IGITAVVLSIQHACDIKLTDLKEAAMEEIIKPVLPNKWLTNNTIFLINPGGRFVIGGPIS
EEHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHCCCCEECCCEEEEECCCCCEEECCCCC
DCGLTGRKIIVDTYGGIGRHGGGSFSGKDPSKVDRSAAYGARYVAKNIVAAGLADRCELQ
CCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
VSYAIGVPHPISMSIETFGTEKISHDILMQLIKNFFDFRPYGLIAMLDLLRPIYKETAVY
EEEEECCCCCCEEEHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GHFGRECFPWEKIDKADLLRDAAGLKCTP
HCCCCCCCCHHHCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA