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The map label for this gene is tyrS

Identifier: 68535935

GI number: 68535935

Start: 1023111

End: 1024397

Strand: Direct

Name: tyrS

Synonym: jk0860

Alternate gene names: 68535935

Gene position: 1023111-1024397 (Clockwise)

Preceding gene: 68535934

Following gene: 68535938

Centisome position: 41.55

GC content: 62.94

Gene sequence:

>1287_bases
ATGAGCGACAACATGAACATCATCGACGAGCTGCAGTGGCGCGGCCTGATTAACCAGTCGACGGACCTGGAGGCGCTGAA
GGAGGCCACGCAGAACCCCATCACGCTGTACTGCGGTTTCGACCCAACGGGCAGCTCCCTGCACGCGGGCCACCTGGTGC
CGCTGATTATGCTGAAGCGCTTCCAGCAGTTCGGCCACCGCCCGATCACCCTGGCTGGTGGCGCGACGGGCATGATTGGC
GACCCGCGCGACGTGGGCGAGCGCTCCATGCTTACTGAGGAGCAGATCGGCGAGAATATGGTGGCCATCAAGAAGCAGCT
GGAGGCATTCGTCGACTTCACCGAGGACAGCGATGTGGATAGCCCGGCCGTCATGGTGAACAACGCGGACTGGATCAGCC
AGATGAACGTCATCGAGTACCTGCGCGATGTCGGCAAGAACTTCTCGCTGAACACCATGCTGGATCGCGATACCGTGAAG
CGCCGCCTGGAATCCGACGGCATTTCCTACACCGAGTTCTCCTACATGCTGCTGCAGGCCAACGACTACGTGCACCTGCA
CAACGAGTTCGATTGCGTGCTGCAGATCGGCGGCGGCGATCAGTGGGGCAACATCGTTTCTGGTGTGGACTTGAACCGCC
GCGTGAACGGCGCCAAGGTGCACGGCCTGACGGTGCCGCTGGTTACGGACGCGGAGGGCAACAAGTTCGGCAAGTCCACC
GGCGGCGGCAAGCTGTGGCTGGATCCGCAGTTGACCAGCCCGTACTCCTGGTACCAGTACTTCATCAACGCGGGCGATTC
CGTGGTGATCGATTACCTGCGCTGGTTCACCTTCCTTTCCCAGGAGGAGATCGCGGAGCTGGAGCGCAAGGTCGCCGAGG
AGCCCTTCAAGCGCGAGGCGCAGCGCGTTCTTGCCCGCGAGATGACCACTCTGGTGCACGGCGCGGCTGCCACCGAGGCT
GTGGAGCTGGCCGCCCAGGCGTTGTTCGGCAAGGCGGAGCTGCAGGATCTGGACGAGCCCACCCTGGCTTCCGCCCTGCA
GGAAACCGAGGTTGCCGAGGTTGGCGCCGATGCCACCATCCTGGACTTGCTCGTCGAGTCCGGCCTGGAAAAGTCCAAGG
GAGCCGCTCGTCGTACTGTCGGGGAAGGCGGCGCTTACGTCAACAACCAGCGCATCGAGGACATCGAATGGTCTCCGTCC
GCCGAGGAACTGCTGCACGGATCGTGGCTCGTGTTGCGCAAGGGCAAGAAGCGTTTCGCCGGTGCGCGCGTAGGTGCTTC
TTCTTAG

Upstream 100 bases:

>100_bases
CTATCCGGTGCAGGACGGCATCCCGGTATTGCTGGTCGAAGAAGCTAAAGACTGGCCCGTTAAATAGCGACGCATTTTAG
AGGAACGAGAACGAGCACGC

Downstream 100 bases:

>100_bases
TGACGCCACCAGCACGGCCTGTATGCGCTAAACCGATCCTCTGGTAGTTGCATTTTCCTGCTTCTACCAGGGGATTTGTG
TCTTTCTTGGCGTTGGGTGT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSDNMNIIDELQWRGLINQSTDLEALKEATQNPITLYCGFDPTGSSLHAGHLVPLIMLKRFQQFGHRPITLAGGATGMIG
DPRDVGERSMLTEEQIGENMVAIKKQLEAFVDFTEDSDVDSPAVMVNNADWISQMNVIEYLRDVGKNFSLNTMLDRDTVK
RRLESDGISYTEFSYMLLQANDYVHLHNEFDCVLQIGGGDQWGNIVSGVDLNRRVNGAKVHGLTVPLVTDAEGNKFGKST
GGGKLWLDPQLTSPYSWYQYFINAGDSVVIDYLRWFTFLSQEEIAELERKVAEEPFKREAQRVLAREMTTLVHGAAATEA
VELAAQALFGKAELQDLDEPTLASALQETEVAEVGADATILDLLVESGLEKSKGAARRTVGEGGAYVNNQRIEDIEWSPS
AEELLHGSWLVLRKGKKRFAGARVGASS

Sequences:

>Translated_428_residues
MSDNMNIIDELQWRGLINQSTDLEALKEATQNPITLYCGFDPTGSSLHAGHLVPLIMLKRFQQFGHRPITLAGGATGMIG
DPRDVGERSMLTEEQIGENMVAIKKQLEAFVDFTEDSDVDSPAVMVNNADWISQMNVIEYLRDVGKNFSLNTMLDRDTVK
RRLESDGISYTEFSYMLLQANDYVHLHNEFDCVLQIGGGDQWGNIVSGVDLNRRVNGAKVHGLTVPLVTDAEGNKFGKST
GGGKLWLDPQLTSPYSWYQYFINAGDSVVIDYLRWFTFLSQEEIAELERKVAEEPFKREAQRVLAREMTTLVHGAAATEA
VELAAQALFGKAELQDLDEPTLASALQETEVAEVGADATILDLLVESGLEKSKGAARRTVGEGGAYVNNQRIEDIEWSPS
AEELLHGSWLVLRKGKKRFAGARVGASS
>Mature_427_residues
SDNMNIIDELQWRGLINQSTDLEALKEATQNPITLYCGFDPTGSSLHAGHLVPLIMLKRFQQFGHRPITLAGGATGMIGD
PRDVGERSMLTEEQIGENMVAIKKQLEAFVDFTEDSDVDSPAVMVNNADWISQMNVIEYLRDVGKNFSLNTMLDRDTVKR
RLESDGISYTEFSYMLLQANDYVHLHNEFDCVLQIGGGDQWGNIVSGVDLNRRVNGAKVHGLTVPLVTDAEGNKFGKSTG
GGKLWLDPQLTSPYSWYQYFINAGDSVVIDYLRWFTFLSQEEIAELERKVAEEPFKREAQRVLAREMTTLVHGAAATEAV
ELAAQALFGKAELQDLDEPTLASALQETEVAEVGADATILDLLVESGLEKSKGAARRTVGEGGAYVNNQRIEDIEWSPSA
EELLHGSWLVLRKGKKRFAGARVGASS

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=399, Percent_Identity=35.8395989974937, Blast_Score=224, Evalue=1e-58,
Organism=Escherichia coli, GI1787925, Length=420, Percent_Identity=47.6190476190476, Blast_Score=377, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI25145802, Length=415, Percent_Identity=34.4578313253012, Blast_Score=206, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI25145805, Length=288, Percent_Identity=34.7222222222222, Blast_Score=150, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6325160, Length=436, Percent_Identity=34.4036697247706, Blast_Score=248, Evalue=1e-66,
Organism=Drosophila melanogaster, GI20130383, Length=417, Percent_Identity=38.8489208633094, Blast_Score=279, Evalue=3e-75,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_CORJK (Q4JVY5)

Other databases:

- EMBL:   CR931997
- RefSeq:   YP_250640.1
- ProteinModelPortal:   Q4JVY5
- SMR:   Q4JVY5
- STRING:   Q4JVY5
- GeneID:   3433066
- GenomeReviews:   CR931997_GR
- KEGG:   cjk:jk0860
- NMPDR:   fig|306537.3.peg.2052
- eggNOG:   COG0162
- HOGENOM:   HBG288125
- OMA:   TFYIGFD
- ProtClustDB:   PRK05912
- BioCyc:   CJEI306537:JK0860-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 47292; Mature: 47161

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 37-37 BINDING 175-175 BINDING 179-179 BINDING 238-238

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNMNIIDELQWRGLINQSTDLEALKEATQNPITLYCGFDPTGSSLHAGHLVPLIMLKR
CCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHH
FQQFGHRPITLAGGATGMIGDPRDVGERSMLTEEQIGENMVAIKKQLEAFVDFTEDSDVD
HHHHCCCCEEEECCCCCCCCCCHHHCHHHHCCHHHHCCHHHHHHHHHHHHHCCCCCCCCC
SPAVMVNNADWISQMNVIEYLRDVGKNFSLNTMLDRDTVKRRLESDGISYTEFSYMLLQA
CCEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEHEEEEEEEC
NDYVHLHNEFDCVLQIGGGDQWGNIVSGVDLNRRVNGAKVHGLTVPLVTDAEGNKFGKST
CCEEEEECCCEEEEEECCCCCCCCHHCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCC
GGGKLWLDPQLTSPYSWYQYFINAGDSVVIDYLRWFTFLSQEEIAELERKVAEEPFKREA
CCCEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHH
QRVLAREMTTLVHGAAATEAVELAAQALFGKAELQDLDEPTLASALQETEVAEVGADATI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHCCCHHH
LDLLVESGLEKSKGAARRTVGEGGAYVNNQRIEDIEWSPSAEELLHGSWLVLRKGKKRFA
HHHHHHHCCCCCCCHHHHHCCCCCCEECCCCCCCCCCCCCHHHHHCCCEEEEECCCHHHC
GARVGASS
CCCCCCCC
>Mature Secondary Structure 
SDNMNIIDELQWRGLINQSTDLEALKEATQNPITLYCGFDPTGSSLHAGHLVPLIMLKR
CCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHH
FQQFGHRPITLAGGATGMIGDPRDVGERSMLTEEQIGENMVAIKKQLEAFVDFTEDSDVD
HHHHCCCCEEEECCCCCCCCCCHHHCHHHHCCHHHHCCHHHHHHHHHHHHHCCCCCCCCC
SPAVMVNNADWISQMNVIEYLRDVGKNFSLNTMLDRDTVKRRLESDGISYTEFSYMLLQA
CCEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEHEEEEEEEC
NDYVHLHNEFDCVLQIGGGDQWGNIVSGVDLNRRVNGAKVHGLTVPLVTDAEGNKFGKST
CCEEEEECCCEEEEEECCCCCCCCHHCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCC
GGGKLWLDPQLTSPYSWYQYFINAGDSVVIDYLRWFTFLSQEEIAELERKVAEEPFKREA
CCCEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHH
QRVLAREMTTLVHGAAATEAVELAAQALFGKAELQDLDEPTLASALQETEVAEVGADATI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHCCCHHH
LDLLVESGLEKSKGAARRTVGEGGAYVNNQRIEDIEWSPSAEELLHGSWLVLRKGKKRFA
HHHHHHHCCCCCCCHHHHHCCCCCCEECCCCCCCCCCCCCHHHHHCCCEEEEECCCHHHC
GARVGASS
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA