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The map label for this gene is argG [H]

Identifier: 68535922

GI number: 68535922

Start: 1008297

End: 1009493

Strand: Direct

Name: argG [H]

Synonym: jk0847

Alternate gene names: 68535922

Gene position: 1008297-1009493 (Clockwise)

Preceding gene: 68535921

Following gene: 68535923

Centisome position: 40.95

GC content: 64.66

Gene sequence:

>1197_bases
ATGAAGGATCGCGTAGTACTCGCATACTCCGGCGGGCTGGATACCACCGTGGCCATCAGCTGGATCGCCAAGGAGCGCAA
CGCAGAAGTCGTCTGCGTCTCCATCGACCTGGGTCAGGGCGGCGAGGACATGGAGACCGTCCGCCAGCGCGCCCTCGGCG
CCGGTGCCGTGGAGTCCATCGTTGTGGACGCTCGCGACGAGTTCGCCAACGACTACTGCCTGCCGACCATCAAGGCCAAC
GGCATGTACATGAAGGAGTACCCGCTGGTCTCCGCGATCTCCCGCCCTCTGATTGTCAAGCACATGGCGGATGCAGCCAA
GGAGCACGGCGGTACCGCGGTTGCTCACGGCTGCACCGGCAAGGGCAATGACCAGGTCCGCTTCGAGGTTGGTTTTGCCA
ATACCGCCCCGGACCTGGAGATTATCGCCCCGGTTCGCGACTACGCCTGGACTCGCGAGAAGGCCATCGCTTTCGCCGAG
GAAAACGGCATCCCGATCGAGCAGTCCAAGTCCTCCCCGTTCTCCATCGACCAGAACGTGTGGGGCCGCGCCGTGGAGAC
CGGCTTCCTGGAGGATCTGTGGAACGCCCCGACCAAGGACGTCTATGCATACACCGAGGATCCGGGCTTGGGCCAGGCTC
CGGATGAGGTCATCATCTCCTTCGAATCCGGCGTGCCAGTGGCCATCGACGGTAAGAAGGTCACCGTACTGGAGGCCATC
GAGGAGCTGAACCGTCGCGCAGGTGCGCAGGGCGTCGGCCGCCTGGACATGGTTGAGGACCGCCTGGTGGGCATCAAGTC
CCGCGAGATCTACGAGGCTCCGGGCGCTATCACCCTGATCCGTGCCCACGAGGCCCTTGAGGCCGTCACCGTCGAGCGCG
AGCTGGCCCGCTACAAGCGCGGCATCGACGCAGAGTGGTCTAACCAGGTCTACGACGGCCTGTGGTTCAGCCCGCTGAAG
CGCTCCCTGGATGCGTTCATCGATTCCACCCAGGCGCACGTCACCGGCGACATCCGCCTGGTGCTGCACGCGGGCTCCAT
CACCGTCAACGGCCGCCGCTCCGGCAAGTCCCTGTACGACTTCAACCTGGCTACCTACGACGAGGGCGACTCCTTCGACC
AGTCCATGGCCCGCGGCTTCGTGGAGCTGCACGGCCTGTCCTCCAAGATCGCCGCTAAGCGCGACCTGGCTAACTAG

Upstream 100 bases:

>100_bases
GAGCTGGGGGAGTACTTCTTCCAGCTGGCACATAGCTAAAATCAAATAACGGATACAACAACTAGAACGCAAGAAGAGTT
TCGAAGGGAAGCTTAGAAGA

Downstream 100 bases:

>100_bases
GCACTGGCACCCCGTAATACACCCCTTCCCGGTGGGGAGTCCCGTTCGCTGGGGCTGCCCGCCGTTGGTTTCTTTAAGAC
CGACATTCTTTTACGACGTC

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MKDRVVLAYSGGLDTTVAISWIAKERNAEVVCVSIDLGQGGEDMETVRQRALGAGAVESIVVDARDEFANDYCLPTIKAN
GMYMKEYPLVSAISRPLIVKHMADAAKEHGGTAVAHGCTGKGNDQVRFEVGFANTAPDLEIIAPVRDYAWTREKAIAFAE
ENGIPIEQSKSSPFSIDQNVWGRAVETGFLEDLWNAPTKDVYAYTEDPGLGQAPDEVIISFESGVPVAIDGKKVTVLEAI
EELNRRAGAQGVGRLDMVEDRLVGIKSREIYEAPGAITLIRAHEALEAVTVERELARYKRGIDAEWSNQVYDGLWFSPLK
RSLDAFIDSTQAHVTGDIRLVLHAGSITVNGRRSGKSLYDFNLATYDEGDSFDQSMARGFVELHGLSSKIAAKRDLAN

Sequences:

>Translated_398_residues
MKDRVVLAYSGGLDTTVAISWIAKERNAEVVCVSIDLGQGGEDMETVRQRALGAGAVESIVVDARDEFANDYCLPTIKAN
GMYMKEYPLVSAISRPLIVKHMADAAKEHGGTAVAHGCTGKGNDQVRFEVGFANTAPDLEIIAPVRDYAWTREKAIAFAE
ENGIPIEQSKSSPFSIDQNVWGRAVETGFLEDLWNAPTKDVYAYTEDPGLGQAPDEVIISFESGVPVAIDGKKVTVLEAI
EELNRRAGAQGVGRLDMVEDRLVGIKSREIYEAPGAITLIRAHEALEAVTVERELARYKRGIDAEWSNQVYDGLWFSPLK
RSLDAFIDSTQAHVTGDIRLVLHAGSITVNGRRSGKSLYDFNLATYDEGDSFDQSMARGFVELHGLSSKIAAKRDLAN
>Mature_398_residues
MKDRVVLAYSGGLDTTVAISWIAKERNAEVVCVSIDLGQGGEDMETVRQRALGAGAVESIVVDARDEFANDYCLPTIKAN
GMYMKEYPLVSAISRPLIVKHMADAAKEHGGTAVAHGCTGKGNDQVRFEVGFANTAPDLEIIAPVRDYAWTREKAIAFAE
ENGIPIEQSKSSPFSIDQNVWGRAVETGFLEDLWNAPTKDVYAYTEDPGLGQAPDEVIISFESGVPVAIDGKKVTVLEAI
EELNRRAGAQGVGRLDMVEDRLVGIKSREIYEAPGAITLIRAHEALEAVTVERELARYKRGIDAEWSNQVYDGLWFSPLK
RSLDAFIDSTQAHVTGDIRLVLHAGSITVNGRRSGKSLYDFNLATYDEGDSFDQSMARGFVELHGLSSKIAAKRDLAN

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=411, Percent_Identity=36.0097323600973, Blast_Score=248, Evalue=7e-66,
Organism=Homo sapiens, GI16950633, Length=411, Percent_Identity=36.0097323600973, Blast_Score=248, Evalue=7e-66,
Organism=Escherichia coli, GI1789563, Length=392, Percent_Identity=29.5918367346939, Blast_Score=115, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6324514, Length=405, Percent_Identity=34.320987654321, Blast_Score=243, Evalue=3e-65,
Organism=Drosophila melanogaster, GI21358151, Length=401, Percent_Identity=35.6608478802993, Blast_Score=252, Evalue=3e-67,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 43413; Mature: 43413

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDRVVLAYSGGLDTTVAISWIAKERNAEVVCVSIDLGQGGEDMETVRQRALGAGAVESI
CCCCEEEEECCCCCCEEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCHHHH
VVDARDEFANDYCLPTIKANGMYMKEYPLVSAISRPLIVKHMADAAKEHGGTAVAHGCTG
EEECHHHHCCCCCCEEEECCCEEEECCCHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCC
KGNDQVRFEVGFANTAPDLEIIAPVRDYAWTREKAIAFAEENGIPIEQSKSSPFSIDQNV
CCCCEEEEEEECCCCCCCCEEEECHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCHHH
WGRAVETGFLEDLWNAPTKDVYAYTEDPGLGQAPDEVIISFESGVPVAIDGKKVTVLEAI
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEECCCEEHHHHHH
EELNRRAGAQGVGRLDMVEDRLVGIKSREIYEAPGAITLIRAHEALEAVTVERELARYKR
HHHHHHCCCCCCCCHHHHHHHHHCCCCCCEECCCCCEEEEEHHHHHHHHHHHHHHHHHHC
GIDAEWSNQVYDGLWFSPLKRSLDAFIDSTQAHVTGDIRLVLHAGSITVNGRRSGKSLYD
CCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECEEEECCCCCCCEEEE
FNLATYDEGDSFDQSMARGFVELHGLSSKIAAKRDLAN
EEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCC
>Mature Secondary Structure
MKDRVVLAYSGGLDTTVAISWIAKERNAEVVCVSIDLGQGGEDMETVRQRALGAGAVESI
CCCCEEEEECCCCCCEEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCHHHH
VVDARDEFANDYCLPTIKANGMYMKEYPLVSAISRPLIVKHMADAAKEHGGTAVAHGCTG
EEECHHHHCCCCCCEEEECCCEEEECCCHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCC
KGNDQVRFEVGFANTAPDLEIIAPVRDYAWTREKAIAFAEENGIPIEQSKSSPFSIDQNV
CCCCEEEEEEECCCCCCCCEEEECHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCHHH
WGRAVETGFLEDLWNAPTKDVYAYTEDPGLGQAPDEVIISFESGVPVAIDGKKVTVLEAI
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEECCCEEHHHHHH
EELNRRAGAQGVGRLDMVEDRLVGIKSREIYEAPGAITLIRAHEALEAVTVERELARYKR
HHHHHHCCCCCCCCHHHHHHHHHCCCCCCEECCCCCEEEEEHHHHHHHHHHHHHHHHHHC
GIDAEWSNQVYDGLWFSPLKRSLDAFIDSTQAHVTGDIRLVLHAGSITVNGRRSGKSLYD
CCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECEEEECCCCCCCEEEE
FNLATYDEGDSFDQSMARGFVELHGLSSKIAAKRDLAN
EEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA