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The map label for this gene is mraW

Identifier: 68535817

GI number: 68535817

Start: 885124

End: 886152

Strand: Direct

Name: mraW

Synonym: jk0742

Alternate gene names: 68535817

Gene position: 885124-886152 (Clockwise)

Preceding gene: 68535816

Following gene: 68535818

Centisome position: 35.94

GC content: 61.32

Gene sequence:

>1029_bases
ATGAGCGAAGAACACGGCCACACCCCAGTCATGCGCGACCGCATGGTCGAACTCGTAGGTCTGGGTGTGAACAGCGAGAA
CGCGCCCGCCCGCCCCGTCATCGTCGACGGCACCCTCGGCGCCGGCGGACACAGCGAGGCTTTTCTTGACGCCTTTCCTC
ACGCAATTGTCGTCGGCCTGGACCGCGACCCCAATGCACTGGCAGAAGCTAATGCGCGTCTGGCACGCTTCGGGGACCGC
TTTGTGTCCTATCAAACCCGTTTCGACGGAGTAACCGAGGCGCTGGAGGACCTGATGGAACAGGGCAAGCTTCCAGCCAG
CGTGCGGGAACACGGTATCTCCGGGTTCCTGTTCGACCTCGGCGTGTCCTCCATGCAGCTGGATCAGCCGGAACGTGGCT
TCGCCTATGCGGTAGACGCTCCGTTGGACATGCGCATGGATCCCAGCAGTCCGCTGACCGCCGCCGAGATCCTGAACACC
TACAGTCACGGCGAGATCGCGCGCATTTTGAAGACCTACGGCGATGAACGCTTCGCCGGAAAGATTGCCTCCGCAGTTGT
CCGCGAGCGTGAAAAGCAGCCCTTCGAGAACTCAGCGCGTTTGGTGGAGCTGTTGTACGCAACCATTCCGGCGGCATCGC
GCCGCACCGGCGGACACCCAGCGAAGCGAACCTTCCAGGCGCTGCGCGTCGAGGTAAACGCTGAACTCGAATCGCTGGAG
AACCTCATTCCGGAGATTTCCCGCTGGCTACATGTTGGTGCCGTGGGAGTGTTTATGAGCTACCAGTCGCTGGAAGACAA
GATCGTCAAGAAAGCGTTGGTGCAGCTCAGTACCTCGAAAACTCCGCCGGGGCTACCGATGGAGCTGCCCGGCATGGAGG
CCGAGTTCGAGCTCAGAACCCGTGGGGCAGAAAAGGCCAGCGAGGCGGAGATTGAACAAAACTCCCGGGCCGCCCCTGTG
CGGGTGAGGGCCTACAGCAGGGTTAGTGACAGAGGAAAAACGATATTTCCGCTGGACACGCCCAGTTAG

Upstream 100 bases:

>100_bases
CACATAAGTATTTATGTGGCACATGAATTGTTTGGAGCCCCAGAAAAGGCTTGTGAACAGGGTCGAAGACAAAACGCAGT
GAAGGAAGGAGGGAACATCG

Downstream 100 bases:

>100_bases
CTAAGCAAAACCCCTAAATGCCACTGATAGAAACAGACCCGAACCTACCGAAACTTCGGAAGGAATCACATGACAGCACC
AGGCACCATCCGCACCCGAG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MSEEHGHTPVMRDRMVELVGLGVNSENAPARPVIVDGTLGAGGHSEAFLDAFPHAIVVGLDRDPNALAEANARLARFGDR
FVSYQTRFDGVTEALEDLMEQGKLPASVREHGISGFLFDLGVSSMQLDQPERGFAYAVDAPLDMRMDPSSPLTAAEILNT
YSHGEIARILKTYGDERFAGKIASAVVREREKQPFENSARLVELLYATIPAASRRTGGHPAKRTFQALRVEVNAELESLE
NLIPEISRWLHVGAVGVFMSYQSLEDKIVKKALVQLSTSKTPPGLPMELPGMEAEFELRTRGAEKASEAEIEQNSRAAPV
RVRAYSRVSDRGKTIFPLDTPS

Sequences:

>Translated_342_residues
MSEEHGHTPVMRDRMVELVGLGVNSENAPARPVIVDGTLGAGGHSEAFLDAFPHAIVVGLDRDPNALAEANARLARFGDR
FVSYQTRFDGVTEALEDLMEQGKLPASVREHGISGFLFDLGVSSMQLDQPERGFAYAVDAPLDMRMDPSSPLTAAEILNT
YSHGEIARILKTYGDERFAGKIASAVVREREKQPFENSARLVELLYATIPAASRRTGGHPAKRTFQALRVEVNAELESLE
NLIPEISRWLHVGAVGVFMSYQSLEDKIVKKALVQLSTSKTPPGLPMELPGMEAEFELRTRGAEKASEAEIEQNSRAAPV
RVRAYSRVSDRGKTIFPLDTPS
>Mature_341_residues
SEEHGHTPVMRDRMVELVGLGVNSENAPARPVIVDGTLGAGGHSEAFLDAFPHAIVVGLDRDPNALAEANARLARFGDRF
VSYQTRFDGVTEALEDLMEQGKLPASVREHGISGFLFDLGVSSMQLDQPERGFAYAVDAPLDMRMDPSSPLTAAEILNTY
SHGEIARILKTYGDERFAGKIASAVVREREKQPFENSARLVELLYATIPAASRRTGGHPAKRTFQALRVEVNAELESLEN
LIPEISRWLHVGAVGVFMSYQSLEDKIVKKALVQLSTSKTPPGLPMELPGMEAEFELRTRGAEKASEAEIEQNSRAAPVR
VRAYSRVSDRGKTIFPLDTPS

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=286, Percent_Identity=37.0629370629371, Blast_Score=158, Evalue=5e-39,
Organism=Homo sapiens, GI165377202, Length=204, Percent_Identity=38.2352941176471, Blast_Score=119, Evalue=4e-27,
Organism=Escherichia coli, GI1786270, Length=334, Percent_Identity=37.125748502994, Blast_Score=177, Evalue=7e-46,
Organism=Drosophila melanogaster, GI62472493, Length=300, Percent_Identity=33, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI28571637, Length=300, Percent_Identity=33, Blast_Score=138, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_CORJK (Q4JWA3)

Other databases:

- EMBL:   CR931997
- RefSeq:   YP_250522.1
- ProteinModelPortal:   Q4JWA3
- SMR:   Q4JWA3
- STRING:   Q4JWA3
- GeneID:   3432488
- GenomeReviews:   CR931997_GR
- KEGG:   cjk:jk0742
- NMPDR:   fig|306537.3.peg.1514
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   RPINSTL
- ProtClustDB:   PRK00050
- BioCyc:   CJEI306537:JK0742-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 37455; Mature: 37324

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: BINDING 61-61 BINDING 88-88 BINDING 119-119 BINDING 126-126

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEHGHTPVMRDRMVELVGLGVNSENAPARPVIVDGTLGAGGHSEAFLDAFPHAIVVGL
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHCCCEEEEEC
DRDPNALAEANARLARFGDRFVSYQTRFDGVTEALEDLMEQGKLPASVREHGISGFLFDL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEHHHH
GVSSMQLDQPERGFAYAVDAPLDMRMDPSSPLTAAEILNTYSHGEIARILKTYGDERFAG
CCCHHCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHH
KIASAVVREREKQPFENSARLVELLYATIPAASRRTGGHPAKRTFQALRVEVNAELESLE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHCHHHHHHH
NLIPEISRWLHVGAVGVFMSYQSLEDKIVKKALVQLSTSKTPPGLPMELPGMEAEFELRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHH
RGAEKASEAEIEQNSRAAPVRVRAYSRVSDRGKTIFPLDTPS
CCCCHHHHHHHHHCCCCCCEEEHHHHHHHCCCCEEECCCCCC
>Mature Secondary Structure 
SEEHGHTPVMRDRMVELVGLGVNSENAPARPVIVDGTLGAGGHSEAFLDAFPHAIVVGL
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHCCCEEEEEC
DRDPNALAEANARLARFGDRFVSYQTRFDGVTEALEDLMEQGKLPASVREHGISGFLFDL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEHHHH
GVSSMQLDQPERGFAYAVDAPLDMRMDPSSPLTAAEILNTYSHGEIARILKTYGDERFAG
CCCHHCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHH
KIASAVVREREKQPFENSARLVELLYATIPAASRRTGGHPAKRTFQALRVEVNAELESLE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHCHHHHHHH
NLIPEISRWLHVGAVGVFMSYQSLEDKIVKKALVQLSTSKTPPGLPMELPGMEAEFELRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHH
RGAEKASEAEIEQNSRAAPVRVRAYSRVSDRGKTIFPLDTPS
CCCCHHHHHHHHHCCCCCCEEEHHHHHHHCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA