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The map label for this gene is ilvE [H]

Identifier: 68535785

GI number: 68535785

Start: 848475

End: 849650

Strand: Direct

Name: ilvE [H]

Synonym: jk0710

Alternate gene names: 68535785

Gene position: 848475-849650 (Clockwise)

Preceding gene: 68535783

Following gene: 68535788

Centisome position: 34.46

GC content: 64.03

Gene sequence:

>1176_bases
ATGACTACTCCAGAGGCAAACACCCAGACCCAAAAGGCACAGCAGAACTCCCAGTTGGAGTTCACCGTAACCCGCAGCCA
GAACCCAGTGGCTGATGAAAAGATTGCCGAGATTCTCTCTGCCCCGGGCTTCGGCAACCACTTCACCGATCACATGGTCG
CCATCGACTGGAACGCGGACGAGGGCTGGCACAACGCTCGCGTGGAGCCCTACCAGCCGGTCCAGATGGACCCGGCATCC
AGCGTGTTCCACTACGGCCAGGCCATCTTCGAAGGTCTGAAGGCATACCGGGGTGAAGGCGGCAAGATCGTCACCTTCCG
CCCGGAGCAGAACGCTCGCCGCTTCCAGAAATCCGCCGCTCGTATGGCCATGCCGGAGCTGCCGGAAGAGCTGTTCCTGG
AATCCCTGCGCCAGATCGTGGACATCGACCAGCGCTGGGTTCCGCAGGGCGGTGGCGAAGAATCCCTGTACCTGCGTCCC
TTCATGATCTCCCGTGAGGTTGGCCTGGGTGTGCACCCGGCTAGCAGCTACACCTACTTTGTGATCGCCTCCCCGGCGGG
CGCTTACTTCTCCGGCGGCATCAACCCAGTGAGCGTCTGGCTATCCACCGACTACACCCGTGCCGCACCCGGCGGTACCG
GTGCTGCGAAGTTCGCCGGTAACTACGCGGCCTCTCTGGCGGCACAGGCTTCCGCAGAGAAGGAAGGCTGCGACCAGGTG
GTATGGCTGGACGCGACCGAGCACAAGTACATCGAAGAGATGGGCGGCATGAACCTGGCGTTCGTATTCGGCAGCGAAGA
CGGTGGCGCCGAGAACGTCAAGCTGGTCACCCCGGAGCTTTCCGGTTCGCTACTGCCGGGCGTTACCCGCCTCTCCCTGC
TGGAGCTGGCGCGCGACCTGGGCTACAAGGTGGAGGAGCGCCGCATCTCCACCGACGAGTGGAAGGAAGCCGCAAAGTCC
GGCGCTATGACCGAGACCTTCGCCTGTGGCACCGCAGCCGTGGTCACCCCGGTCGGCACGGTCAAGGACGAGTCCGGCGA
ATTCCAGGTCAACGGTGGCCAGACTGGCCCGGTGACCATGCGCCTGCGCGAGCAGCTGACGGGCATTCAGAATGGCCGCG
TCGCCGACAAGCACGGCTGGCTCTACACGCTGGTGGAGCAGGCAGCCACCGCGTAG

Upstream 100 bases:

>100_bases
TACCCAGATTCGCCTGCCGGCAGATCGCCGGGAGTAACCACTGCCGAGAAATTGTTAGCCCCCTCACACTTTTTTGTAGC
AGCAGAGTATTCTGGGTCAC

Downstream 100 bases:

>100_bases
CGCACCATCGGCAGGGCCAGGACAGCACCTAGCCCCTGGCCGGTGCCGAGCTGTAAATCTAGAAGCGGCTCCAGGCCTAG
CGCATTGAGCGCCGGCAGGG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MTTPEANTQTQKAQQNSQLEFTVTRSQNPVADEKIAEILSAPGFGNHFTDHMVAIDWNADEGWHNARVEPYQPVQMDPAS
SVFHYGQAIFEGLKAYRGEGGKIVTFRPEQNARRFQKSAARMAMPELPEELFLESLRQIVDIDQRWVPQGGGEESLYLRP
FMISREVGLGVHPASSYTYFVIASPAGAYFSGGINPVSVWLSTDYTRAAPGGTGAAKFAGNYAASLAAQASAEKEGCDQV
VWLDATEHKYIEEMGGMNLAFVFGSEDGGAENVKLVTPELSGSLLPGVTRLSLLELARDLGYKVEERRISTDEWKEAAKS
GAMTETFACGTAAVVTPVGTVKDESGEFQVNGGQTGPVTMRLREQLTGIQNGRVADKHGWLYTLVEQAATA

Sequences:

>Translated_391_residues
MTTPEANTQTQKAQQNSQLEFTVTRSQNPVADEKIAEILSAPGFGNHFTDHMVAIDWNADEGWHNARVEPYQPVQMDPAS
SVFHYGQAIFEGLKAYRGEGGKIVTFRPEQNARRFQKSAARMAMPELPEELFLESLRQIVDIDQRWVPQGGGEESLYLRP
FMISREVGLGVHPASSYTYFVIASPAGAYFSGGINPVSVWLSTDYTRAAPGGTGAAKFAGNYAASLAAQASAEKEGCDQV
VWLDATEHKYIEEMGGMNLAFVFGSEDGGAENVKLVTPELSGSLLPGVTRLSLLELARDLGYKVEERRISTDEWKEAAKS
GAMTETFACGTAAVVTPVGTVKDESGEFQVNGGQTGPVTMRLREQLTGIQNGRVADKHGWLYTLVEQAATA
>Mature_390_residues
TTPEANTQTQKAQQNSQLEFTVTRSQNPVADEKIAEILSAPGFGNHFTDHMVAIDWNADEGWHNARVEPYQPVQMDPASS
VFHYGQAIFEGLKAYRGEGGKIVTFRPEQNARRFQKSAARMAMPELPEELFLESLRQIVDIDQRWVPQGGGEESLYLRPF
MISREVGLGVHPASSYTYFVIASPAGAYFSGGINPVSVWLSTDYTRAAPGGTGAAKFAGNYAASLAAQASAEKEGCDQVV
WLDATEHKYIEEMGGMNLAFVFGSEDGGAENVKLVTPELSGSLLPGVTRLSLLELARDLGYKVEERRISTDEWKEAAKSG
AMTETFACGTAAVVTPVGTVKDESGEFQVNGGQTGPVTMRLREQLTGIQNGRVADKHGWLYTLVEQAATA

Specific function: Catalyzes the reversible transfers of an amino group from glutamate to the alpha-ketoacid of the respective amino acid in the final step in the biosynthesis of branchedchain amino acids. The amino acids can be ranked in the following order with respect to

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010904, Length=333, Percent_Identity=42.3423423423423, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI296010906, Length=333, Percent_Identity=42.3423423423423, Blast_Score=263, Evalue=3e-70,
Organism=Homo sapiens, GI38176287, Length=333, Percent_Identity=42.3423423423423, Blast_Score=263, Evalue=3e-70,
Organism=Homo sapiens, GI50658084, Length=370, Percent_Identity=37.8378378378378, Blast_Score=254, Evalue=9e-68,
Organism=Homo sapiens, GI296010902, Length=333, Percent_Identity=37.2372372372372, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI296010900, Length=333, Percent_Identity=37.2372372372372, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI258614015, Length=297, Percent_Identity=38.047138047138, Blast_Score=202, Evalue=4e-52,
Organism=Escherichia coli, GI48994963, Length=334, Percent_Identity=32.9341317365269, Blast_Score=145, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17568601, Length=347, Percent_Identity=40.0576368876081, Blast_Score=250, Evalue=9e-67,
Organism=Caenorhabditis elegans, GI17565728, Length=346, Percent_Identity=34.971098265896, Blast_Score=191, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6322002, Length=363, Percent_Identity=40.2203856749311, Blast_Score=243, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6322608, Length=344, Percent_Identity=37.7906976744186, Blast_Score=228, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24641779, Length=342, Percent_Identity=39.1812865497076, Blast_Score=229, Evalue=2e-60,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 42483; Mature: 42352

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPEANTQTQKAQQNSQLEFTVTRSQNPVADEKIAEILSAPGFGNHFTDHMVAIDWNAD
CCCCCCCCHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCC
EGWHNARVEPYQPVQMDPASSVFHYGQAIFEGLKAYRGEGGKIVTFRPEQNARRFQKSAA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHH
RMAMPELPEELFLESLRQIVDIDQRWVPQGGGEESLYLRPFMISREVGLGVHPASSYTYF
HHHCCCCCHHHHHHHHHHHHCCHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCEEEE
VIASPAGAYFSGGINPVSVWLSTDYTRAAPGGTGAAKFAGNYAASLAAQASAEKEGCDQV
EEECCCCCHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHCHHHHHHHHHHCCCCCCCCEE
VWLDATEHKYIEEMGGMNLAFVFGSEDGGAENVKLVTPELSGSLLPGVTRLSLLELARDL
EEEECHHHHHHHHHCCCEEEEEEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHC
GYKVEERRISTDEWKEAAKSGAMTETFACGTAAVVTPVGTVKDESGEFQVNGGQTGPVTM
CCEEHHHCCCHHHHHHHHHCCCCCEECCCCCCCEECCCCCEECCCCCEEECCCCCCCCHH
RLREQLTGIQNGRVADKHGWLYTLVEQAATA
HHHHHHCCCCCCCEECCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TTPEANTQTQKAQQNSQLEFTVTRSQNPVADEKIAEILSAPGFGNHFTDHMVAIDWNAD
CCCCCCCHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCC
EGWHNARVEPYQPVQMDPASSVFHYGQAIFEGLKAYRGEGGKIVTFRPEQNARRFQKSAA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHH
RMAMPELPEELFLESLRQIVDIDQRWVPQGGGEESLYLRPFMISREVGLGVHPASSYTYF
HHHCCCCCHHHHHHHHHHHHCCHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCEEEE
VIASPAGAYFSGGINPVSVWLSTDYTRAAPGGTGAAKFAGNYAASLAAQASAEKEGCDQV
EEECCCCCHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHCHHHHHHHHHHCCCCCCCCEE
VWLDATEHKYIEEMGGMNLAFVFGSEDGGAENVKLVTPELSGSLLPGVTRLSLLELARDL
EEEECHHHHHHHHHCCCEEEEEEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHC
GYKVEERRISTDEWKEAAKSGAMTETFACGTAAVVTPVGTVKDESGEFQVNGGQTGPVTM
CCEEHHHCCCHHHHHHHHHCCCCCEECCCCCCCEECCCCCEECCCCCEEECCCCCCCCHH
RLREQLTGIQNGRVADKHGWLYTLVEQAATA
HHHHHHCCCCCCCEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA